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The map label for this gene is proW [H]

Identifier: 152971536

GI number: 152971536

Start: 3308031

End: 3309098

Strand: Direct

Name: proW [H]

Synonym: KPN_03009

Alternate gene names: 152971536

Gene position: 3308031-3309098 (Clockwise)

Preceding gene: 152971535

Following gene: 152971537

Centisome position: 62.24

GC content: 65.36

Gene sequence:

>1068_bases
ATGGCTGATCAGACCAATCCATGGGGCAGCGCCCCGTCAACCGACAGCGCCTCGCAGGCTGCCGACGCCTGGGGTGGCGC
CTCTACTGCCGCGCCGGCTAATGGCGGCGGCGCCGACTGGCTGCACAGCGCCCCGGCGCCGCAGCCGGAACAGTTCAATA
TCATGGATCCGTTCCATAAGACCCTGATCCCGCTCGACAGCTGGGTGACCCACGCCATCGACTGGATCGTGCTGCACTTC
CGTCCGCTGTTCCAGGGGATCCGCGTGCCGATCGACTACATCCTCAGCGCCTTCCAGCAGCTGCTGTTGGGGATGCCGGC
GCCGGTGGCGATTATCGTCTTCGCGCTAATCGCCTGGCAGATCTCCAGCCTCGGCATGGGGGTCGCCACTCTGGTATCGC
TGATTGCCATCGGCGCCATCGGCGCGTGGTCGCAAGCCATGGTCACTCTGGCGCTGGTCCTCACCGCCCTGCTGTTCTGC
ATGGTTATCGGCTTGCCGCTGGGGATCTGGCTAGCGCGCAGCCCGCGGGCGGCGAAAATCATCCGCCCGCTGTTGGATGC
CATGCAGACCACGCCGGCGTTCGTCTATCTGGTGCCGATCGTGATGCTGTTCGGCATCGGCAACGTACCGGGCGTGGTGG
TGACGATTATCTTCGCCCTGCCGCCGATCGTCCGTCTGACCATCCTCGGGATAAACCAGGTGCCTGCGGATCTGATCGAA
GCGTCGCGCTCATTTGGCGCCAGCCCGCGCCAGCTGCTGTTTAAGGTGCAGCTGCCGCTGGCCATGCCCACCATTATGGC
CGGGGTTAACCAGACCCTGATGCTGGCCCTGTCGATGGTAGTGATCGCCTCGATGATCGCCGTCGGCGGCCTCGGCCAGA
TGGTGCTGCGCGGTATCGGTCGCCTGGATATGGGCCTGGCGACCGTCGGCGGGGTGGGGATCGTGATCCTGGCGATTATT
CTTGACCGCCTCACCCAGGCCGTCGGTCGCGACGCCCGCAGTCGCGGCAACCGCCGCTGGTACACCACCGGCCCGCTGGG
GCTTATCACTCGCCCTTTCTGTCGATAA

Upstream 100 bases:

>100_bases
GCTCCGTGCGCCGTGCCGGTAGTGGATGAAGAACAACAATATATTGGCATCATTTCCAAACGGATGTTGCTCCAGGCTTT
AGATCGCGAGGGGGTGAATC

Downstream 100 bases:

>100_bases
GTCGATAACAGCGCCGCTCCCCGACACCAGCCGATGCCCGGGAGCGGCCCTTCCCGTTAAACAATAAGAGGAACCACCAT
GCGACATAGTACGATGTTGG

Product: glycine betaine transporter membrane protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MADQTNPWGSAPSTDSASQAADAWGGASTAAPANGGGADWLHSAPAPQPEQFNIMDPFHKTLIPLDSWVTHAIDWIVLHF
RPLFQGIRVPIDYILSAFQQLLLGMPAPVAIIVFALIAWQISSLGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFC
MVIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIVRLTILGINQVPADLIE
ASRSFGASPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAII
LDRLTQAVGRDARSRGNRRWYTTGPLGLITRPFCR

Sequences:

>Translated_355_residues
MADQTNPWGSAPSTDSASQAADAWGGASTAAPANGGGADWLHSAPAPQPEQFNIMDPFHKTLIPLDSWVTHAIDWIVLHF
RPLFQGIRVPIDYILSAFQQLLLGMPAPVAIIVFALIAWQISSLGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFC
MVIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIVRLTILGINQVPADLIE
ASRSFGASPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAII
LDRLTQAVGRDARSRGNRRWYTTGPLGLITRPFCR
>Mature_354_residues
ADQTNPWGSAPSTDSASQAADAWGGASTAAPANGGGADWLHSAPAPQPEQFNIMDPFHKTLIPLDSWVTHAIDWIVLHFR
PLFQGIRVPIDYILSAFQQLLLGMPAPVAIIVFALIAWQISSLGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCM
VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIVRLTILGINQVPADLIEA
SRSFGASPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIIL
DRLTQAVGRDARSRGNRRWYTTGPLGLITRPFCR

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=355, Percent_Identity=89.0140845070423, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=32.9842931937173, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788449, Length=184, Percent_Identity=30.4347826086957, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786564, Length=154, Percent_Identity=31.8181818181818, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 37657; Mature: 37526

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQTNPWGSAPSTDSASQAADAWGGASTAAPANGGGADWLHSAPAPQPEQFNIMDPFHK
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHH
TLIPLDSWVTHAIDWIVLHFRPLFQGIRVPIDYILSAFQQLLLGMPAPVAIIVFALIAWQ
HHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ISSLGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCMVIGLPLGIWLARSPRAAKI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIVRLTILGINQVPADLIE
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ASRSFGASPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIG
HHHHCCCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
RLDMGLATVGGVGIVILAIILDRLTQAVGRDARSRGNRRWYTTGPLGLITRPFCR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEECCCHHHCCCCCCC
>Mature Secondary Structure 
ADQTNPWGSAPSTDSASQAADAWGGASTAAPANGGGADWLHSAPAPQPEQFNIMDPFHK
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHH
TLIPLDSWVTHAIDWIVLHFRPLFQGIRVPIDYILSAFQQLLLGMPAPVAIIVFALIAWQ
HHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ISSLGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCMVIGLPLGIWLARSPRAAKI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIVRLTILGINQVPADLIE
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ASRSFGASPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIG
HHHHCCCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
RLDMGLATVGGVGIVILAIILDRLTQAVGRDARSRGNRRWYTTGPLGLITRPFCR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEECCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]