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The map label for this gene is setB [H]

Identifier: 152971140

GI number: 152971140

Start: 2856007

End: 2857188

Strand: Direct

Name: setB [H]

Synonym: KPN_02601

Alternate gene names: 152971140

Gene position: 2856007-2857188 (Clockwise)

Preceding gene: 152971136

Following gene: 152971142

Centisome position: 53.73

GC content: 60.58

Gene sequence:

>1182_bases
ATGGAAAACCGTAGCGCCGCTCTCCCCCGACGCGGGTTCGATGTCACCTCTTCCGCCTTCTTAATCGTCGCCTTTCTGAC
GGGCATTGCCGGGGCGCTGCAAACCCCGACCCTGAGCCTGTTTCTGACCAACGAAGTGCACGTCCGCCCGGCGATGGTCG
GTTTCTTTTTTACCGGCAGCGCGGTTATTGGCATTCTGGTCAGTCAGTTCCTCGCCGGCCGCTCCGACCGTCAGGGCGAT
CGCAAGCAGCTGATTGTCGTCTGCTGTCTGCTGGGGGTGCTGGCCTGCGTACTCTTTGCCTGGAACCGCAACTATTTCAT
TTTGCTGTTCATCGGCGTTTTTCTCAGTAGCTTTGGATCGACCGCCAACCCGCAGATGTTTGCCCTCGCGCGTGAGCATG
CCGACAGGACCGGCCGCGAAGCGGTGATGTTCAGCTCCATTTTACGTGCCCAGGTCTCCCTGGCATGGGTGATTGGTCCC
CCGCTGGCCTACGCCCTGGCGATGGGGTTTGGTTTTACCGCGATGTATCTCAGCGCCGCGGCGGCGTTTATCGTCTGCGG
GATCATGGTCTGGCTATTCTTGCCCAGCATGCGCAAAGCAAAGCCGGTAGCGACCGGCCGGCTGGAAGCTCCGCGCACGC
ACCGCCGCGATGCGCTGCTGCTGTTCAGCATCTGCACCCTGATGTGGGGTACTAACAGCCTGTACATCATTAACATGCCG
CTGTTTATCATTAACGAGCTGCATCTGCCGGAGAAGCTGGCGGGGCTGATGATGGGCACCGCCGCCGGACTGGAAATTCC
GACCATGCTGATTGCCGGTTACTACGCCCGGCGTTTCGGCAAACGCTTTCTGATGCGTCTTTCTGCAGTGGCCGGGGTGC
TGTTTTACGTCGGCATGCTGACGGTGCATACGCCAGCGCTGCTGCTGGCGATGCAGGTGCTGAACGCCATCTATATCGGG
ATCCTCGCCGGGATCGGCATGCTCTATTTCCAGGACCTGATGCCGGGGCAGGCGGGCGCGGCCACCACCCTCTATACCAA
TACTACCCGCGTCGGCTGGATCATCGCCGGTTCACTGGCGGGGGTAGTGGCGGAGATCTGGAGCTATCACGCGGTGTTCT
GGATTGCGCTGGCGATGGGCGTTGTCACTCAGGCCTGCCTGTGGCGGATAAGAGACGTGTAA

Upstream 100 bases:

>100_bases
TTGAAACGCCTTTTTAATTATTCGTGCCATACTACTGTTTATTTTTAGCCAGCAGGCGTAAACTCCGCGCCGTCTCCTGA
AACTACAGAAAACCAAACCC

Downstream 100 bases:

>100_bases
GCACATTGGTTTCCATCCCTCTCATCTGCTGCGTTGAGCTCAGGGCGCGGTGGCCAGCTCAAGCTCCAGCAGCCAGGTGA
TCGCTTCATCGCGGTGGGTG

Product: major facilitator superfamily lactose/glucose:proton efflux pump

Products: lactose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MENRSAALPRRGFDVTSSAFLIVAFLTGIAGALQTPTLSLFLTNEVHVRPAMVGFFFTGSAVIGILVSQFLAGRSDRQGD
RKQLIVVCCLLGVLACVLFAWNRNYFILLFIGVFLSSFGSTANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGP
PLAYALAMGFGFTAMYLSAAAAFIVCGIMVWLFLPSMRKAKPVATGRLEAPRTHRRDALLLFSICTLMWGTNSLYIINMP
LFIINELHLPEKLAGLMMGTAAGLEIPTMLIAGYYARRFGKRFLMRLSAVAGVLFYVGMLTVHTPALLLAMQVLNAIYIG
ILAGIGMLYFQDLMPGQAGAATTLYTNTTRVGWIIAGSLAGVVAEIWSYHAVFWIALAMGVVTQACLWRIRDV

Sequences:

>Translated_393_residues
MENRSAALPRRGFDVTSSAFLIVAFLTGIAGALQTPTLSLFLTNEVHVRPAMVGFFFTGSAVIGILVSQFLAGRSDRQGD
RKQLIVVCCLLGVLACVLFAWNRNYFILLFIGVFLSSFGSTANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGP
PLAYALAMGFGFTAMYLSAAAAFIVCGIMVWLFLPSMRKAKPVATGRLEAPRTHRRDALLLFSICTLMWGTNSLYIINMP
LFIINELHLPEKLAGLMMGTAAGLEIPTMLIAGYYARRFGKRFLMRLSAVAGVLFYVGMLTVHTPALLLAMQVLNAIYIG
ILAGIGMLYFQDLMPGQAGAATTLYTNTTRVGWIIAGSLAGVVAEIWSYHAVFWIALAMGVVTQACLWRIRDV
>Mature_393_residues
MENRSAALPRRGFDVTSSAFLIVAFLTGIAGALQTPTLSLFLTNEVHVRPAMVGFFFTGSAVIGILVSQFLAGRSDRQGD
RKQLIVVCCLLGVLACVLFAWNRNYFILLFIGVFLSSFGSTANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGP
PLAYALAMGFGFTAMYLSAAAAFIVCGIMVWLFLPSMRKAKPVATGRLEAPRTHRRDALLLFSICTLMWGTNSLYIINMP
LFIINELHLPEKLAGLMMGTAAGLEIPTMLIAGYYARRFGKRFLMRLSAVAGVLFYVGMLTVHTPALLLAMQVLNAIYIG
ILAGIGMLYFQDLMPGQAGAATTLYTNTTRVGWIIAGSLAGVVAEIWSYHAVFWIALAMGVVTQACLWRIRDV

Specific function: Involved in the efflux of sugars. The physiological role may be the detoxification of non-metabolizable sugar analogs. Can transport lactose and glucose [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Set transporter family [H]

Homologues:

Organism=Escherichia coli, GI1788495, Length=393, Percent_Identity=81.9338422391858, Blast_Score=650, Evalue=0.0,
Organism=Escherichia coli, GI1790091, Length=393, Percent_Identity=67.4300254452926, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI48994875, Length=384, Percent_Identity=50.2604166666667, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004750 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42862; Mature: 42862

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENRSAALPRRGFDVTSSAFLIVAFLTGIAGALQTPTLSLFLTNEVHVRPAMVGFFFTGS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECHHHHHHHHHHH
AVIGILVSQFLAGRSDRQGDRKQLIVVCCLLGVLACVLFAWNRNYFILLFIGVFLSSFGS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCC
TANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGPPLAYALAMGFGFTAMYLSAA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHH
AAFIVCGIMVWLFLPSMRKAKPVATGRLEAPRTHRRDALLLFSICTLMWGTNSLYIINMP
HHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECH
LFIINELHLPEKLAGLMMGTAAGLEIPTMLIAGYYARRFGKRFLMRLSAVAGVLFYVGML
HHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVHTPALLLAMQVLNAIYIGILAGIGMLYFQDLMPGQAGAATTLYTNTTRVGWIIAGSLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEHHEEHHHHH
GVVAEIWSYHAVFWIALAMGVVTQACLWRIRDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MENRSAALPRRGFDVTSSAFLIVAFLTGIAGALQTPTLSLFLTNEVHVRPAMVGFFFTGS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECHHHHHHHHHHH
AVIGILVSQFLAGRSDRQGDRKQLIVVCCLLGVLACVLFAWNRNYFILLFIGVFLSSFGS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCC
TANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGPPLAYALAMGFGFTAMYLSAA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHH
AAFIVCGIMVWLFLPSMRKAKPVATGRLEAPRTHRRDALLLFSICTLMWGTNSLYIINMP
HHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECH
LFIINELHLPEKLAGLMMGTAAGLEIPTMLIAGYYARRFGKRFLMRLSAVAGVLFYVGML
HHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVHTPALLLAMQVLNAIYIGILAGIGMLYFQDLMPGQAGAATTLYTNTTRVGWIIAGSLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEHHEEHHHHH
GVVAEIWSYHAVFWIALAMGVVTQACLWRIRDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: lactose [Cytoplasm] + Proton [Periplasm] = lactose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 10209755; 10438463 [H]