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The map label for this gene is nfo [H]

Identifier: 152971136

GI number: 152971136

Start: 2850985

End: 2851839

Strand: Direct

Name: nfo [H]

Synonym: KPN_02597

Alternate gene names: 152971136

Gene position: 2850985-2851839 (Clockwise)

Preceding gene: 152971135

Following gene: 152971140

Centisome position: 53.64

GC content: 60.58

Gene sequence:

>855_bases
ATGAAATACGTAGGAGCGCACGTGAGCGCCTCAGGTGGACTGGCCAATGCCGCCATTCGCGCCGCCGAAATCGAAGCAAC
CGCCTTCGCCCTCTTCACCAAGAACCAGCGCCAGTGGCGCGCCGCGCCGCTCAGCGACGAGACCATCGCCGAATTCAAAG
CCGCCTGTGAAAAGTATCACTTTGGCCCGGGGCAGATCCTGCCGCACGACAGCTACCTGATTAACCTTGGCCACCCGGTG
GAAGAAGCGCTGGAAAAATCCCGTGATGCCTTCATTGATGAAATGACCCGCTGCCAGCAGCTGGGACTGACGCTGCTCAA
CTTCCACCCCGGCAGCCATCTGCAGCAGATCCCGGAGGAGGAGTGCCTGGCGCGCATCGCCGAATCGATCAATATCGCGC
TGGCGAAGACCGAAGGCGTTACTGCGGTGATCGAAAACACCGCTGGCCAGGGCAGCAACCTCGGCTTTAAGTTCGAGCAC
CTCGCGGCCATCATCGACGGTGTGGAAGATAAATCCCGCGTCGGCGTCTGCATCGATACCTGCCATGCTTTCGCCGCCGG
CTACGACCTGCGCAGCGCCGAAGCCTGCGAAAAAACCTTCGCCGCCTTCGAACGCATCGTCGGTTTTCAGTATCTGCGCG
GCATGCATCTCAACGATGCCAAGAGCGCCTTCGGCAGCCGGGTCGACCGTCACCACAGCCTGGGGGAAGGCAACATCGGC
CACGATTGCTTCAGCTGGATCATGCAGGACAGCCGCTTCGACGGTATTCCGCTGATTCTGGAAACCATCAACCCGGATAT
CTGGGCGGAAGAGATTGCCTGGCTGCGGGCGCAGCAGATTGCCGAAGTGGCGTAA

Upstream 100 bases:

>100_bases
CGTCGCTATCCACAGCCTGATGGCATAAAGCACTACATCTTTCTCCAGTTAAGCCGCTATCATTACCCACCCCCCAGCGG
CTTTAACTGGAGTTTTTTTT

Downstream 100 bases:

>100_bases
ATAGAGACAAAAAAGGGCCGAACAGTCGGCCCTTTTTTATTGCGGTAGGATTATGCCGCTTTTTCAACTTCCGCTGTTTG
CGCCTCAGGGCGTTTCAGCA

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKYVGAHVSASGGLANAAIRAAEIEATAFALFTKNQRQWRAAPLSDETIAEFKAACEKYHFGPGQILPHDSYLINLGHPV
EEALEKSRDAFIDEMTRCQQLGLTLLNFHPGSHLQQIPEEECLARIAESINIALAKTEGVTAVIENTAGQGSNLGFKFEH
LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRSAEACEKTFAAFERIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HDCFSWIMQDSRFDGIPLILETINPDIWAEEIAWLRAQQIAEVA

Sequences:

>Translated_284_residues
MKYVGAHVSASGGLANAAIRAAEIEATAFALFTKNQRQWRAAPLSDETIAEFKAACEKYHFGPGQILPHDSYLINLGHPV
EEALEKSRDAFIDEMTRCQQLGLTLLNFHPGSHLQQIPEEECLARIAESINIALAKTEGVTAVIENTAGQGSNLGFKFEH
LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRSAEACEKTFAAFERIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HDCFSWIMQDSRFDGIPLILETINPDIWAEEIAWLRAQQIAEVA
>Mature_284_residues
MKYVGAHVSASGGLANAAIRAAEIEATAFALFTKNQRQWRAAPLSDETIAEFKAACEKYHFGPGQILPHDSYLINLGHPV
EEALEKSRDAFIDEMTRCQQLGLTLLNFHPGSHLQQIPEEECLARIAESINIALAKTEGVTAVIENTAGQGSNLGFKFEH
LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRSAEACEKTFAAFERIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HDCFSWIMQDSRFDGIPLILETINPDIWAEEIAWLRAQQIAEVA

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=279, Percent_Identity=88.1720430107527, Blast_Score=520, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17531193, Length=261, Percent_Identity=48.2758620689655, Blast_Score=270, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6322735, Length=285, Percent_Identity=40, Blast_Score=231, Evalue=7e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 31183; Mature: 31183

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYVGAHVSASGGLANAAIRAAEIEATAFALFTKNQRQWRAAPLSDETIAEFKAACEKYH
CCEECCEEECCCCHHHHHHHHHHHHHHEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHCC
FGPGQILPHDSYLINLGHPVEEALEKSRDAFIDEMTRCQQLGLTLLNFHPGSHLQQIPEE
CCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHCCHH
ECLARIAESINIALAKTEGVTAVIENTAGQGSNLGFKFEHLAAIIDGVEDKSRVGVCIDT
HHHHHHHHHHEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHH
CHAFAAGYDLRSAEACEKTFAAFERIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
HDCFSWIMQDSRFDGIPLILETINPDIWAEEIAWLRAQQIAEVA
HHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYVGAHVSASGGLANAAIRAAEIEATAFALFTKNQRQWRAAPLSDETIAEFKAACEKYH
CCEECCEEECCCCHHHHHHHHHHHHHHEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHCC
FGPGQILPHDSYLINLGHPVEEALEKSRDAFIDEMTRCQQLGLTLLNFHPGSHLQQIPEE
CCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHCCHH
ECLARIAESINIALAKTEGVTAVIENTAGQGSNLGFKFEHLAAIIDGVEDKSRVGVCIDT
HHHHHHHHHHEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHH
CHAFAAGYDLRSAEACEKTFAAFERIVGFQYLRGMHLNDAKSAFGSRVDRHHSLGEGNIG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
HDCFSWIMQDSRFDGIPLILETINPDIWAEEIAWLRAQQIAEVA
HHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA