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The map label for this gene is purT [H]

Identifier: 152970911

GI number: 152970911

Start: 2587400

End: 2588578

Strand: Direct

Name: purT [H]

Synonym: KPN_02364

Alternate gene names: 152970911

Gene position: 2587400-2588578 (Clockwise)

Preceding gene: 152970907

Following gene: 152970915

Centisome position: 48.68

GC content: 63.7

Gene sequence:

>1179_bases
ATGACTGTATTAGGCACCGCGCTGCGCCCGGCAGCCACCAAAGTTATGCTGTTAGGTTCCGGCGAGTTGGGTAAAGAAGT
CGCTATTGAATGTCAGCGTCTGGGCATTGAAACCATCGCCGTAGACCGCTACCCCGATGCCCCGGCGATGCAGGTCGCCC
ACCGCGCCCACGTCATCAATATGCTGCACGGCGAGAGCCTGCGCGCCCTGATTGAACAGGAAAAACCTGATTATATCGTG
CCGGAGATCGAAGCTATCGCTACCGACACCCTGGTCGAACTGGAACAGGCCGGGCAGAAAGTGGTCCCCACGGCGCGGGC
GGCAAAGCTCACCATGAACCGCGAAGGCATTCGCCGCCTGGCGGCGGAAGAGCTGCAGCTTCCCACCTCCCGCTATCGTT
TTGCCGACAGCGAAGAAGGCTTCCGCGCGGCGGTTACCGAGATCGGTTTGCCATGCATCGTTAAACCGGTGATGAGCTCC
TCCGGCAAAGGCCAGAGCTTTATCCGCTCGGCTGACCAGCTCAGCGAAGCCTGGCGCTATGCGCAGCAGGGCGGCCGCGC
TGGCGCCGGACGGGTGATCGTCGAGGGGGTGGTGAACTTCGATTTCGAAATCACCCTGCTCACCGTCAGCGCTGTCGATG
GCGTGCATTTCTGCGCTCCGGTTGGCCATCGTCAGGAAGACGGCGATTACCGCGAGTCCTGGCAGCCGCAGCAGATGAGC
GACCTGGCCCTCGAGCGTGCCCAGGCCATCGCCCGCGAGGTGGTGCTGGCGCTGGGAGGTTATGGTCTGTTTGGCGTGGA
GCTGTTCGTATGCGGCGACGAGGTGATCTTCAGCGAAGTCTCCCCGCGACCACACGACACCGGGATGGTGACGCTTATCT
CCCAGGATCTCTCGGAATTCGCCCTGCACGTTCGCGCCTTCCTCGGGCTACCCGTCGGCGCTATCCGCCAGTATGGTCCG
GCCGCCTCCGCGGTGATCCTTCCGCAGTTGTCCAGCCAGAACGTCAGCTTTGGCCAGCTGCAGTCGGCCGTCGGGGCTGG
CCTGCAGCTGCGTCTGTTCGGCAAGCCGGAGATTGACGGTACCCGCCGCCTGGGGGTAACCCTCGCCGTCGCCGATTCGG
TTGAGGAGGCCGTGGCGCGCGCTAAAGCCGCCGCCGCGGCGGTCATCGTCGAAGGGTAA

Upstream 100 bases:

>100_bases
TCCCTCCCGGACTCAACTCACGCGCGTTACGCGCGTAAAGACATACGCAAACGTTTTCGTTTATAATCGCTGCCATCTTT
TTTTACTGCGGATGAGAGTT

Downstream 100 bases:

>100_bases
AGACAAAAAACCCGGTCGCGTTGCCGCAGACCGGGTTGTTGACCTGGAGGCGTGGTTTATTTCGCGCCTTCCACCGCTTC
TCGCGCGAGGGTGGTGATGC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MTVLGTALRPAATKVMLLGSGELGKEVAIECQRLGIETIAVDRYPDAPAMQVAHRAHVINMLHGESLRALIEQEKPDYIV
PEIEAIATDTLVELEQAGQKVVPTARAAKLTMNREGIRRLAAEELQLPTSRYRFADSEEGFRAAVTEIGLPCIVKPVMSS
SGKGQSFIRSADQLSEAWRYAQQGGRAGAGRVIVEGVVNFDFEITLLTVSAVDGVHFCAPVGHRQEDGDYRESWQPQQMS
DLALERAQAIAREVVLALGGYGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGAIRQYGP
AASAVILPQLSSQNVSFGQLQSAVGAGLQLRLFGKPEIDGTRRLGVTLAVADSVEEAVARAKAAAAAVIVEG

Sequences:

>Translated_392_residues
MTVLGTALRPAATKVMLLGSGELGKEVAIECQRLGIETIAVDRYPDAPAMQVAHRAHVINMLHGESLRALIEQEKPDYIV
PEIEAIATDTLVELEQAGQKVVPTARAAKLTMNREGIRRLAAEELQLPTSRYRFADSEEGFRAAVTEIGLPCIVKPVMSS
SGKGQSFIRSADQLSEAWRYAQQGGRAGAGRVIVEGVVNFDFEITLLTVSAVDGVHFCAPVGHRQEDGDYRESWQPQQMS
DLALERAQAIAREVVLALGGYGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGAIRQYGP
AASAVILPQLSSQNVSFGQLQSAVGAGLQLRLFGKPEIDGTRRLGVTLAVADSVEEAVARAKAAAAAVIVEG
>Mature_391_residues
TVLGTALRPAATKVMLLGSGELGKEVAIECQRLGIETIAVDRYPDAPAMQVAHRAHVINMLHGESLRALIEQEKPDYIVP
EIEAIATDTLVELEQAGQKVVPTARAAKLTMNREGIRRLAAEELQLPTSRYRFADSEEGFRAAVTEIGLPCIVKPVMSSS
GKGQSFIRSADQLSEAWRYAQQGGRAGAGRVIVEGVVNFDFEITLLTVSAVDGVHFCAPVGHRQEDGDYRESWQPQQMSD
LALERAQAIAREVVLALGGYGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGAIRQYGPA
ASAVILPQLSSQNVSFGQLQSAVGAGLQLRLFGKPEIDGTRRLGVTLAVADSVEEAVARAKAAAAAVIVEG

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=392, Percent_Identity=84.1836734693878, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI1786733, Length=269, Percent_Identity=25.2788104089219, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324702, Length=382, Percent_Identity=23.5602094240838, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 41981; Mature: 41849

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLGTALRPAATKVMLLGSGELGKEVAIECQRLGIETIAVDRYPDAPAMQVAHRAHVIN
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
MLHGESLRALIEQEKPDYIVPEIEAIATDTLVELEQAGQKVVPTARAAKLTMNREGIRRL
HHCCHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHEEEECHHHHHHH
AAEELQLPTSRYRFADSEEGFRAAVTEIGLPCIVKPVMSSSGKGQSFIRSADQLSEAWRY
HHHHHCCCHHHHCCCCCCCCHHHHHHHCCCHHEEHHHHCCCCCCHHHHHHHHHHHHHHHH
AQQGGRAGAGRVIVEGVVNFDFEITLLTVSAVDGVHFCAPVGHRQEDGDYRESWQPQQMS
HHCCCCCCCCCEEEEEECCCCEEEEEEEEEHHCCCCEECCCCCCCCCCCHHHCCCCHHHH
DLALERAQAIAREVVLALGGYGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEF
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHCCCCCCCCCCEEEHHHHHHHHH
ALHVRAFLGLPVGAIRQYGPAASAVILPQLSSQNVSFGQLQSAVGAGLQLRLFGKPEIDG
HHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCCC
TRRLGVTLAVADSVEEAVARAKAAAAAVIVEG
CCEEEEEEEEHHHHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure 
TVLGTALRPAATKVMLLGSGELGKEVAIECQRLGIETIAVDRYPDAPAMQVAHRAHVIN
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
MLHGESLRALIEQEKPDYIVPEIEAIATDTLVELEQAGQKVVPTARAAKLTMNREGIRRL
HHCCHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHEEEECHHHHHHH
AAEELQLPTSRYRFADSEEGFRAAVTEIGLPCIVKPVMSSSGKGQSFIRSADQLSEAWRY
HHHHHCCCHHHHCCCCCCCCHHHHHHHCCCHHEEHHHHCCCCCCHHHHHHHHHHHHHHHH
AQQGGRAGAGRVIVEGVVNFDFEITLLTVSAVDGVHFCAPVGHRQEDGDYRESWQPQQMS
HHCCCCCCCCCEEEEEECCCCEEEEEEEEEHHCCCCEECCCCCCCCCCCHHHCCCCHHHH
DLALERAQAIAREVVLALGGYGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEF
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHCCCCCCCCCCEEEHHHHHHHHH
ALHVRAFLGLPVGAIRQYGPAASAVILPQLSSQNVSFGQLQSAVGAGLQLRLFGKPEIDG
HHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCCC
TRRLGVTLAVADSVEEAVARAKAAAAAVIVEG
CCEEEEEEEEHHHHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA