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The map label for this gene is dadA2 [H]

Identifier: 152970721

GI number: 152970721

Start: 2381091

End: 2382344

Strand: Direct

Name: dadA2 [H]

Synonym: KPN_02172

Alternate gene names: 152970721

Gene position: 2381091-2382344 (Clockwise)

Preceding gene: 152970720

Following gene: 152970722

Centisome position: 44.8

GC content: 58.77

Gene sequence:

>1254_bases
ATGAACAAACGCATTGTCATTATCGGCGGCGGCGTGGTCGGTCTGGCCACGGCCTGGGAACTGATCAAGCGCGGCCATCA
GGTGCAATTACTGGAGCGCAATGCCGAACCGGGCAGCGCCACCAGTTTTGCCAACGGCGGACAATTAAGTTACCGCTACG
TCGCCCCCCTTGCCGACAGCGGCGTTCCTCTGCAGGGAATGAAATGGATGGGCAAAGCCGATTCGCCGCTGAATATGCGT
CTCAGGATGTCTCTCCAGCAGTGGCGCTGGCTGCTGCAGTTTCTGCGCGCCTGTAATAACCAGACCAATAAAATGAATGG
CGACCATATTTTACGCCTGTCGCTGCTCAGCCGTCAGGTCATGCAGGCGTGGCGGGATGAGGATAATCTTGCCGATTTCC
ACTGGCGGCGCAGCGGAAAATTAATTATCCATCGTCGCGAATATGATTTTAATAAAGCAGCAAAAGGGATTGATCCTCAG
TATCAGCAGGCGCTGAATGCTGAAGCCTGCCTGCAGCTGGAGCCGGCGCTCAAACATATTAGTCCTTCTCTGCAGGGCGG
AATCTATTCGCCAGGCGATGAAACGGCAGATTGTCATCAGTTCTGCCTTGCCCTGCTCGACAAGCTCAATGCCAGCAGCG
ACTTTAGCCTGCTGACCCAGTGTGAGGTGCGGCGACTCCATAAGCGCGGGGGACGCATCAGCAGCCTGGAGACCAGTCAG
GGGACCCTCACCGGCGATGAGTATGTCGTCGCCGCCGGTAACGGCAGTGGTTCTCTGCTTGGGCATCTGGGGGTTCGCGT
GCCGCTGTGCGCCCTGAAGGGGTACAGCCTGACCCTGCCCTATCCTGAAAAAGCAGGTATTGCCCCTGACATCAGCGTCA
CCGACTACGGGCACAAAATCGTTTATGCCCGCCTTGGCCAACAGCTGCGCATCGCGGCGATGGTGGACATCGGCTACGAC
GGCGACGAACTGCGCGAATGCCGCATTCAGGCGCTGAAGAACATCGTCGCCCGCAGCTTTCCTGAACTGGAGGGGCTGGA
CGAGGCCGAAGTCTGGACCGGCATGCGCCCGTCAACGCCAGCCGGCCCGCCGATGCTGGGGCGTGCCGGCTACCCCAACC
TGTGGATGAACCTCGGCCAGGGTAGCCTCGGCTTTACTCTCGCCGCCGGCAGCGCGGTGGTGCTGGGCGCGTTAATCGAC
AATCAGATGCCAGACATTTCTTTAGAAGGTCTCACATGGAAACAAACCGCATGA

Upstream 100 bases:

>100_bases
AACCTGCGGTTATACCCTGACCATAAAAAGTCAATTGCGCCGCTGGGGTAAATGCTTTGACATAACCCGATAAATTTATC
AGCGGTGGATATATCGGGTT

Downstream 100 bases:

>100_bases
CAATTATTCGTAATAATCCACAACCTCGTCTTGCCGCCAGCGTTGCGTATGGCGATCTGCTGTTCCTCTCCGGGCAGACG
CCAAAAAGCAATGAAGACGA

Product: putative glycine/D-amino acid oxidases (deaminating)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MNKRIVIIGGGVVGLATAWELIKRGHQVQLLERNAEPGSATSFANGGQLSYRYVAPLADSGVPLQGMKWMGKADSPLNMR
LRMSLQQWRWLLQFLRACNNQTNKMNGDHILRLSLLSRQVMQAWRDEDNLADFHWRRSGKLIIHRREYDFNKAAKGIDPQ
YQQALNAEACLQLEPALKHISPSLQGGIYSPGDETADCHQFCLALLDKLNASSDFSLLTQCEVRRLHKRGGRISSLETSQ
GTLTGDEYVVAAGNGSGSLLGHLGVRVPLCALKGYSLTLPYPEKAGIAPDISVTDYGHKIVYARLGQQLRIAAMVDIGYD
GDELRECRIQALKNIVARSFPELEGLDEAEVWTGMRPSTPAGPPMLGRAGYPNLWMNLGQGSLGFTLAAGSAVVLGALID
NQMPDISLEGLTWKQTA

Sequences:

>Translated_417_residues
MNKRIVIIGGGVVGLATAWELIKRGHQVQLLERNAEPGSATSFANGGQLSYRYVAPLADSGVPLQGMKWMGKADSPLNMR
LRMSLQQWRWLLQFLRACNNQTNKMNGDHILRLSLLSRQVMQAWRDEDNLADFHWRRSGKLIIHRREYDFNKAAKGIDPQ
YQQALNAEACLQLEPALKHISPSLQGGIYSPGDETADCHQFCLALLDKLNASSDFSLLTQCEVRRLHKRGGRISSLETSQ
GTLTGDEYVVAAGNGSGSLLGHLGVRVPLCALKGYSLTLPYPEKAGIAPDISVTDYGHKIVYARLGQQLRIAAMVDIGYD
GDELRECRIQALKNIVARSFPELEGLDEAEVWTGMRPSTPAGPPMLGRAGYPNLWMNLGQGSLGFTLAAGSAVVLGALID
NQMPDISLEGLTWKQTA
>Mature_417_residues
MNKRIVIIGGGVVGLATAWELIKRGHQVQLLERNAEPGSATSFANGGQLSYRYVAPLADSGVPLQGMKWMGKADSPLNMR
LRMSLQQWRWLLQFLRACNNQTNKMNGDHILRLSLLSRQVMQAWRDEDNLADFHWRRSGKLIIHRREYDFNKAAKGIDPQ
YQQALNAEACLQLEPALKHISPSLQGGIYSPGDETADCHQFCLALLDKLNASSDFSLLTQCEVRRLHKRGGRISSLETSQ
GTLTGDEYVVAAGNGSGSLLGHLGVRVPLCALKGYSLTLPYPEKAGIAPDISVTDYGHKIVYARLGQQLRIAAMVDIGYD
GDELRECRIQALKNIVARSFPELEGLDEAEVWTGMRPSTPAGPPMLGRAGYPNLWMNLGQGSLGFTLAAGSAVVLGALID
NQMPDISLEGLTWKQTA

Specific function: Oxidative deamination of D-amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=416, Percent_Identity=35.5769230769231, Blast_Score=245, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 45751; Mature: 45751

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRIVIIGGGVVGLATAWELIKRGHQVQLLERNAEPGSATSFANGGQLSYRYVAPLADS
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCC
GVPLQGMKWMGKADSPLNMRLRMSLQQWRWLLQFLRACNNQTNKMNGDHILRLSLLSRQV
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHH
MQAWRDEDNLADFHWRRSGKLIIHRREYDFNKAAKGIDPQYQQALNAEACLQLEPALKHI
HHHHCCCCCCCHHEEECCCCEEEEECCCCHHHHHCCCCHHHHHHCCHHHHEEECHHHHHC
SPSLQGGIYSPGDETADCHQFCLALLDKLNASSDFSLLTQCEVRRLHKRGGRISSLETSQ
CHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
GTLTGDEYVVAAGNGSGSLLGHLGVRVPLCALKGYSLTLPYPEKAGIAPDISVTDYGHKI
CCCCCCCEEEEECCCCCCHHHHHCCCCEEEEECCCEEECCCCCCCCCCCCCEEECCCCEE
VYARLGQQLRIAAMVDIGYDGDELRECRIQALKNIVARSFPELEGLDEAEVWTGMRPSTP
EEECCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCC
AGPPMLGRAGYPNLWMNLGQGSLGFTLAAGSAVVLGALIDNQMPDISLEGLTWKQTA
CCCCCCCCCCCCHHHHCCCCCCCCEEEECCCHHHHHHHHCCCCCCCEECCEEECCCC
>Mature Secondary Structure
MNKRIVIIGGGVVGLATAWELIKRGHQVQLLERNAEPGSATSFANGGQLSYRYVAPLADS
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCC
GVPLQGMKWMGKADSPLNMRLRMSLQQWRWLLQFLRACNNQTNKMNGDHILRLSLLSRQV
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHH
MQAWRDEDNLADFHWRRSGKLIIHRREYDFNKAAKGIDPQYQQALNAEACLQLEPALKHI
HHHHCCCCCCCHHEEECCCCEEEEECCCCHHHHHCCCCHHHHHHCCHHHHEEECHHHHHC
SPSLQGGIYSPGDETADCHQFCLALLDKLNASSDFSLLTQCEVRRLHKRGGRISSLETSQ
CHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
GTLTGDEYVVAAGNGSGSLLGHLGVRVPLCALKGYSLTLPYPEKAGIAPDISVTDYGHKI
CCCCCCCEEEEECCCCCCHHHHHCCCCEEEEECCCEEECCCCCCCCCCCCCEEECCCCEE
VYARLGQQLRIAAMVDIGYDGDELRECRIQALKNIVARSFPELEGLDEAEVWTGMRPSTP
EEECCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCC
AGPPMLGRAGYPNLWMNLGQGSLGFTLAAGSAVVLGALIDNQMPDISLEGLTWKQTA
CCCCCCCCCCCCHHHHCCCCCCCCEEEECCCHHHHHHHHCCCCCCCEECCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]