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The map label for this gene is vanB [H]

Identifier: 152970584

GI number: 152970584

Start: 2230013

End: 2230906

Strand: Direct

Name: vanB [H]

Synonym: KPN_02035

Alternate gene names: 152970584

Gene position: 2230013-2230906 (Clockwise)

Preceding gene: 152970579

Following gene: 152970585

Centisome position: 41.96

GC content: 61.07

Gene sequence:

>894_bases
TTGCAGACGGCGGCCGGGGAAATATTACCGGCCTACTCCGCCGGGGCGCATATCGATATTATTATTCCCGGCGTCGGCTC
CCGGCAGTATTCCCTGTGCGGCACGCCTGACCGCAGTAATACTTATGAAATTTGCGTCAGGCTGACTGACACCTCCACCG
GCGGCTCACGCTATTTGCACCAGCAGCTCAAAGCCGGCGATCGGCTCGCCATTTCGCCGCCGCGCAACCATTTTCCCCTG
CCGCAGGCCGGGCGCTACCTGCTGTTTGCCGGCGGTATCGGCATCACGCCTTTGCTGGCGATGGCTGAAGCGATTGCCGC
CAGAAAGGGGGCGCTGGAGCTGCATTATTATGTCGCCAGTTCGCGCCAGACGGCCTTCTCCCCGCGCCTGAACCAGCTGA
CTGCGAACGGCACGGTGGCCATTCACTGCAGCGAGGAAGGCGCGTCGCTTCGCCAGCGGGTCCCCGAATGCTTAACTGTG
CCCCACCCTGATACCGCAGTAATAGCCTGCGGCCCGGAGGGTTTTATTCAGCGACTGCGGTCGGTCATGGAGGAATATCG
CTGGTCGCCGTCGCAGTTCGTATTCGAGCGTTTTACTCCCGCGGCGGAAAATAATACTGCGGCGAAAAATGCGTTTTATA
TTGAGCTGGCCTCCAGCGGGCAGCGCCTGCAGGTGGCCGCGGATCAAACCATTGCTCAGGTATTACAGCACGCCGGGGTG
GAGGTAATGCTCTCCTGCGAACAGGGAATGTGCGGCTCCTGTATTACCGGGGTCCTCGACGGGATCCCCGAGCACCGGGA
CAGCGTGCTGACGGCGGAAGAGAAAGCCGGCAACGACCAGATCACGCTCTGCTGTTCGCGGGCGAAATCGCCGGGTCTGG
TTTTGGATCTGTGA

Upstream 100 bases:

>100_bases
GCGGAATGAGGGAGAGTAAACGGTAATGCACAGCCACGAACTTATTTCGGTCTCGGTCGGGGAAATACGCCCCAACGGAC
AGGGAAACCTATCCATAATT

Downstream 100 bases:

>100_bases
GGAGACTGACGATGACGCTCCCCTCCTCTTGTGCGCCGCTCACCGGCATCAGCCGCGCCCGACTCCCGGCCTGGGCGCTG
CCCGACGGCTGGCCGACGCA

Product: putative vanillate O-demethylase oxidoreductase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MQTAAGEILPAYSAGAHIDIIIPGVGSRQYSLCGTPDRSNTYEICVRLTDTSTGGSRYLHQQLKAGDRLAISPPRNHFPL
PQAGRYLLFAGGIGITPLLAMAEAIAARKGALELHYYVASSRQTAFSPRLNQLTANGTVAIHCSEEGASLRQRVPECLTV
PHPDTAVIACGPEGFIQRLRSVMEEYRWSPSQFVFERFTPAAENNTAAKNAFYIELASSGQRLQVAADQTIAQVLQHAGV
EVMLSCEQGMCGSCITGVLDGIPEHRDSVLTAEEKAGNDQITLCCSRAKSPGLVLDL

Sequences:

>Translated_297_residues
MQTAAGEILPAYSAGAHIDIIIPGVGSRQYSLCGTPDRSNTYEICVRLTDTSTGGSRYLHQQLKAGDRLAISPPRNHFPL
PQAGRYLLFAGGIGITPLLAMAEAIAARKGALELHYYVASSRQTAFSPRLNQLTANGTVAIHCSEEGASLRQRVPECLTV
PHPDTAVIACGPEGFIQRLRSVMEEYRWSPSQFVFERFTPAAENNTAAKNAFYIELASSGQRLQVAADQTIAQVLQHAGV
EVMLSCEQGMCGSCITGVLDGIPEHRDSVLTAEEKAGNDQITLCCSRAKSPGLVLDL
>Mature_297_residues
MQTAAGEILPAYSAGAHIDIIIPGVGSRQYSLCGTPDRSNTYEICVRLTDTSTGGSRYLHQQLKAGDRLAISPPRNHFPL
PQAGRYLLFAGGIGITPLLAMAEAIAARKGALELHYYVASSRQTAFSPRLNQLTANGTVAIHCSEEGASLRQRVPECLTV
PHPDTAVIACGPEGFIQRLRSVMEEYRWSPSQFVFERFTPAAENNTAAKNAFYIELASSGQRLQVAADQTIAQVLQHAGV
EVMLSCEQGMCGSCITGVLDGIPEHRDSVLTAEEKAGNDQITLCCSRAKSPGLVLDL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=300, Percent_Identity=39.3333333333333, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787098, Length=242, Percent_Identity=26.0330578512397, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 31902; Mature: 31902

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTAAGEILPAYSAGAHIDIIIPGVGSRQYSLCGTPDRSNTYEICVRLTDTSTGGSRYLH
CCCCCCCEEECCCCCCEEEEEECCCCCCCEEECCCCCCCCCEEEEEEEECCCCCHHHHHH
QQLKAGDRLAISPPRNHFPLPQAGRYLLFAGGIGITPLLAMAEAIAARKGALELHYYVAS
HHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEC
SRQTAFSPRLNQLTANGTVAIHCSEEGASLRQRVPECLTVPHPDTAVIACGPEGFIQRLR
CCCCCCCCCHHEEECCCEEEEEECCCCHHHHHCCHHHCCCCCCCCEEEEECCHHHHHHHH
SVMEEYRWSPSQFVFERFTPAAENNTAAKNAFYIELASSGQRLQVAADQTIAQVLQHAGV
HHHHHHCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHCCE
EVMLSCEQGMCGSCITGVLDGIPEHRDSVLTAEEKAGNDQITLCCSRAKSPGLVLDL
EEEEECCCCCCHHHHHHHHHCCCCHHCCEEEEHHHCCCCEEEEEEECCCCCCEEEEC
>Mature Secondary Structure
MQTAAGEILPAYSAGAHIDIIIPGVGSRQYSLCGTPDRSNTYEICVRLTDTSTGGSRYLH
CCCCCCCEEECCCCCCEEEEEECCCCCCCEEECCCCCCCCCEEEEEEEECCCCCHHHHHH
QQLKAGDRLAISPPRNHFPLPQAGRYLLFAGGIGITPLLAMAEAIAARKGALELHYYVAS
HHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEC
SRQTAFSPRLNQLTANGTVAIHCSEEGASLRQRVPECLTVPHPDTAVIACGPEGFIQRLR
CCCCCCCCCHHEEECCCEEEEEECCCCHHHHHCCHHHCCCCCCCCEEEEECCHHHHHHHH
SVMEEYRWSPSQFVFERFTPAAENNTAAKNAFYIELASSGQRLQVAADQTIAQVLQHAGV
HHHHHHCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHCCE
EVMLSCEQGMCGSCITGVLDGIPEHRDSVLTAEEKAGNDQITLCCSRAKSPGLVLDL
EEEEECCCCCCHHHHHHHHHCCCCHHCCEEEEHHHCCCCEEEEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3170489 [H]