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The map label for this gene is ycdT [H]

Identifier: 152970532

GI number: 152970532

Start: 2176042

End: 2177217

Strand: Direct

Name: ycdT [H]

Synonym: KPN_01980

Alternate gene names: 152970532

Gene position: 2176042-2177217 (Clockwise)

Preceding gene: 152970531

Following gene: 152970534

Centisome position: 40.94

GC content: 51.19

Gene sequence:

>1176_bases
ATGGGCATGAAATACTGGAGCAGCAAGCGGCGTCTGTACCTTACTCCGGTCTCTTTCGGGTTTGCCTGCTCAGCGCTGCT
GATGCTTGGCACGCTCAGCAGCTATCCGGACTGGCTTACCTGCAATCCGGCCCCGGTCGTTAATCAAAATGACGCCGTTA
TCTATTATTTCTTCCGCAATATAATGATGGCTGTGTTATTCATGTCATCTATTATTTTGTACTATTTTCGCCAGCGCATC
ATGCATTCGTGGAAAGCACACGTTCTTACATTTACAGCATGTATACTTTTTACGTTGACCATTATTGTTTTATCATGGCT
ATACTCCAGCCATTCGCCGTGGCTGAGCGTCAATTTTATCGACGATCTGAGCCATACCTTTACCCCGTTGTGGCAAAGTA
TTATCGGCTGGCTGCTGATGGCAGTTTGGTTCATCACGCTGATTTTATTAATTAGCTTGAGTAAACTGCGTAATATTTTT
TGGTTCAGCGGCGCTTTTTTTTGCAGCGCTTATTTGTTCACGCTTTTCCAGCTGCTCTCCACCGCAGGGGAGCTGGATCA
GACCTGGTACCAGGCGCGTTTTTTCGAAACGCTATGCACCTTGTTCCTGATTCTGGTGCTACTGGTTGACGTCTTTATCC
TCTATCGCGAGTCTAACCACAAATATGTCCATTCGTATCAGAATTCGATCCGCGACCCGTTGACCCGCCTCTACAACCGC
AGCTTTTTCTACGACACTCTTAACCAACAGCTGGCGAAGGTGAATGCCCAGCATCCGCTGTCGGTGCTCATCAGCGACCT
CGACCATTTCAAACGCATCAACGATAGCTACGGCCATGTCGCGGGCGACAAAGTGATCCAGTTCGCCGCCTCGGTGCTGG
AAAGCCATTCGCGGGTAGATGACGCCGCCGCACGCATCGGCGGTGAGGAATTTGCTCTGCTGCTGGTCAACACCGGTGAA
AAAGAAGCGCAAGCGATCGCCGAGCGTATCCGCCTGGCGGTCAGTGCCGGGGAGAGCCATTTACCGGAGCGAATGACCAT
CAGCATGGGGGTGTATACCACCTACGACAATAGCGTGACAGCGGAAGCGTGCGTTCAGCGCGCGGACGAAGCCATGTATG
AAGCAAAGAATAACGGACGTAACCAGGTGATCGTCTGGCATCAGCAGGGAGGGTGA

Upstream 100 bases:

>100_bases
GACTGCTTTTTTTCATACCAGCTTCAGTAGTACTTCCTCAATATTATTTCCGCTGCTGACCAGTTTCCTGATGGTTTTTC
ATATTACCATTGCCTGTTTT

Downstream 100 bases:

>100_bases
AAAAAGGCCCGGATTAGCCCGGGCCGCAAAGAATTAATCTTTAGCCTGGAGATCGAGGATATTTTGTTCAAGTTTACGGA
TTAAGTGGAGCAAGGTTTCC

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MGMKYWSSKRRLYLTPVSFGFACSALLMLGTLSSYPDWLTCNPAPVVNQNDAVIYYFFRNIMMAVLFMSSIILYYFRQRI
MHSWKAHVLTFTACILFTLTIIVLSWLYSSHSPWLSVNFIDDLSHTFTPLWQSIIGWLLMAVWFITLILLISLSKLRNIF
WFSGAFFCSAYLFTLFQLLSTAGELDQTWYQARFFETLCTLFLILVLLVDVFILYRESNHKYVHSYQNSIRDPLTRLYNR
SFFYDTLNQQLAKVNAQHPLSVLISDLDHFKRINDSYGHVAGDKVIQFAASVLESHSRVDDAAARIGGEEFALLLVNTGE
KEAQAIAERIRLAVSAGESHLPERMTISMGVYTTYDNSVTAEACVQRADEAMYEAKNNGRNQVIVWHQQGG

Sequences:

>Translated_391_residues
MGMKYWSSKRRLYLTPVSFGFACSALLMLGTLSSYPDWLTCNPAPVVNQNDAVIYYFFRNIMMAVLFMSSIILYYFRQRI
MHSWKAHVLTFTACILFTLTIIVLSWLYSSHSPWLSVNFIDDLSHTFTPLWQSIIGWLLMAVWFITLILLISLSKLRNIF
WFSGAFFCSAYLFTLFQLLSTAGELDQTWYQARFFETLCTLFLILVLLVDVFILYRESNHKYVHSYQNSIRDPLTRLYNR
SFFYDTLNQQLAKVNAQHPLSVLISDLDHFKRINDSYGHVAGDKVIQFAASVLESHSRVDDAAARIGGEEFALLLVNTGE
KEAQAIAERIRLAVSAGESHLPERMTISMGVYTTYDNSVTAEACVQRADEAMYEAKNNGRNQVIVWHQQGG
>Mature_390_residues
GMKYWSSKRRLYLTPVSFGFACSALLMLGTLSSYPDWLTCNPAPVVNQNDAVIYYFFRNIMMAVLFMSSIILYYFRQRIM
HSWKAHVLTFTACILFTLTIIVLSWLYSSHSPWLSVNFIDDLSHTFTPLWQSIIGWLLMAVWFITLILLISLSKLRNIFW
FSGAFFCSAYLFTLFQLLSTAGELDQTWYQARFFETLCTLFLILVLLVDVFILYRESNHKYVHSYQNSIRDPLTRLYNRS
FFYDTLNQQLAKVNAQHPLSVLISDLDHFKRINDSYGHVAGDKVIQFAASVLESHSRVDDAAARIGGEEFALLLVNTGEK
EAQAIAERIRLAVSAGESHLPERMTISMGVYTTYDNSVTAEACVQRADEAMYEAKNNGRNQVIVWHQQGG

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=349, Percent_Identity=30.6590257879656, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1788085, Length=393, Percent_Identity=27.2264631043257, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87082007, Length=199, Percent_Identity=38.1909547738693, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788381, Length=165, Percent_Identity=40, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87081881, Length=156, Percent_Identity=41.6666666666667, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=36.8098159509202, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787802, Length=158, Percent_Identity=33.5443037974684, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787541, Length=157, Percent_Identity=31.8471337579618, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787816, Length=165, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=32.0987654320988, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081974, Length=160, Percent_Identity=33.125, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788956, Length=163, Percent_Identity=31.9018404907975, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787056, Length=178, Percent_Identity=29.2134831460674, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081977, Length=178, Percent_Identity=28.6516853932584, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 44916; Mature: 44785

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
>Mature Secondary Structure 
GMKYWSSKRRLYLTPVSFGFACSALLMLGTLSSYPDWLTCNPAPVVNQNDAVIYYFFRN
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEHHHHHH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HHHHHHHHHHHHHHHHCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]