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The map label for this gene is pcaJ [H]

Identifier: 152970091

GI number: 152970091

Start: 1710236

End: 1710892

Strand: Direct

Name: pcaJ [H]

Synonym: KPN_01539

Alternate gene names: 152970091

Gene position: 1710236-1710892 (Clockwise)

Preceding gene: 152970090

Following gene: 152970092

Centisome position: 32.18

GC content: 63.62

Gene sequence:

>657_bases
ATGCAAAAACTGACCCGTGACGAAATGGCCCAGCGCGTGGCCCGCGATATTCCCGAAGGCGCTTACGTCAACCTCGGCAT
CGGCCTGCCGACCCGCATCGCCAATTACCTGCCCGCTGACAAAGAGGTGTTCCTGCACAGCGAAAACGGCCTGCTGGGGA
TGGGGCCGAAACCGCAGCCCGGGGAAGAAGATCCCGAGCTGATCAATGCCGGTAAAGAGTATGTCACCCTCCTGCAGGGG
GGCTGCTATTTCCACCACGGCGACTCCTTCGCCATGATGCGCGGCGGCCATCTCGATATCTGCGTGCTGGGGGCCTATCA
GGTCTCCGCCAGCGGCGACCTCGCCAACTGGAGCACCGGTGCGCCGGATGCGATCCCGGCCGTTGGCGGGGCGATGGATC
TGGCCATCGGCGCCCGCCAGGTGTTTGTGATGATGGACCACCTGACCCGTGACGGCGAGTGCAAGCTGGTGGCGCAGTGC
AGCTACCCGCTGACCGGCGTCGGCTGCGTCAGCCGGATCTATACCGACCTGGCGGTGATCGACATCACCGACCGCGGCCC
GGTGGTGCGGGAAATCTTTAACGGCCTCTCTTTTGAAGAGCTGCAGCGCATCACTCCGGTGGCGCTGACCTTTCAACAAC
TGGCGGAAAGCGCGTAA

Upstream 100 bases:

>100_bases
GACCTCGACCCGGAAAACATCATCACCCCCGGCATTTTCGTCCAGCGCGTCTTCTCGCTGGAAAACCTGACTGCCGCACA
ACGCGCCTGAGGAGCCCGAC

Downstream 100 bases:

>100_bases
GGAACCTATGATGAATCAGGCATTTATCTGTGATGCGGTGCGTACACCCTTTGGCCGTTTCGGCGGTGCCCTGGCGACGA
TGCGCGCTGACGACCTGGCG

Product: acetyl-CoA:acetoacetyl-CoA transferase beta subunit

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MQKLTRDEMAQRVARDIPEGAYVNLGIGLPTRIANYLPADKEVFLHSENGLLGMGPKPQPGEEDPELINAGKEYVTLLQG
GCYFHHGDSFAMMRGGHLDICVLGAYQVSASGDLANWSTGAPDAIPAVGGAMDLAIGARQVFVMMDHLTRDGECKLVAQC
SYPLTGVGCVSRIYTDLAVIDITDRGPVVREIFNGLSFEELQRITPVALTFQQLAESA

Sequences:

>Translated_218_residues
MQKLTRDEMAQRVARDIPEGAYVNLGIGLPTRIANYLPADKEVFLHSENGLLGMGPKPQPGEEDPELINAGKEYVTLLQG
GCYFHHGDSFAMMRGGHLDICVLGAYQVSASGDLANWSTGAPDAIPAVGGAMDLAIGARQVFVMMDHLTRDGECKLVAQC
SYPLTGVGCVSRIYTDLAVIDITDRGPVVREIFNGLSFEELQRITPVALTFQQLAESA
>Mature_218_residues
MQKLTRDEMAQRVARDIPEGAYVNLGIGLPTRIANYLPADKEVFLHSENGLLGMGPKPQPGEEDPELINAGKEYVTLLQG
GCYFHHGDSFAMMRGGHLDICVLGAYQVSASGDLANWSTGAPDAIPAVGGAMDLAIGARQVFVMMDHLTRDGECKLVAQC
SYPLTGVGCVSRIYTDLAVIDITDRGPVVREIFNGLSFEELQRITPVALTFQQLAESA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI11545841, Length=206, Percent_Identity=44.6601941747573, Blast_Score=181, Evalue=5e-46,
Organism=Homo sapiens, GI4557817, Length=219, Percent_Identity=42.0091324200913, Blast_Score=177, Evalue=5e-45,
Organism=Escherichia coli, GI1788552, Length=200, Percent_Identity=41.5, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17531517, Length=204, Percent_Identity=47.5490196078431, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24655794, Length=181, Percent_Identity=48.6187845303867, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI21358325, Length=181, Percent_Identity=48.6187845303867, Blast_Score=176, Evalue=1e-44,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 23498; Mature: 23498

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLTRDEMAQRVARDIPEGAYVNLGIGLPTRIANYLPADKEVFLHSENGLLGMGPKPQP
CCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCEEEEECCCCEECCCCCCCC
GEEDPELINAGKEYVTLLQGGCYFHHGDSFAMMRGGHLDICVLGAYQVSASGDLANWSTG
CCCCHHHHHCCHHHHHHHHCCEEEEECCCEEEEECCCEEEEEEEEEEEECCCCCCCCCCC
APDAIPAVGGAMDLAIGARQVFVMMDHLTRDGECKLVAQCSYPLTGVGCVSRIYTDLAVI
CCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHEEEE
DITDRGPVVREIFNGLSFEELQRITPVALTFQQLAESA
EECCCCHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHCC
>Mature Secondary Structure
MQKLTRDEMAQRVARDIPEGAYVNLGIGLPTRIANYLPADKEVFLHSENGLLGMGPKPQP
CCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCEEEEECCCCEECCCCCCCC
GEEDPELINAGKEYVTLLQGGCYFHHGDSFAMMRGGHLDICVLGAYQVSASGDLANWSTG
CCCCHHHHHCCHHHHHHHHCCEEEEECCCEEEEECCCEEEEEEEEEEEECCCCCCCCCCC
APDAIPAVGGAMDLAIGARQVFVMMDHLTRDGECKLVAQCSYPLTGVGCVSRIYTDLAVI
CCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHEEEE
DITDRGPVVREIFNGLSFEELQRITPVALTFQQLAESA
EECCCCHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8063101; 8125318 [H]