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The map label for this gene is astE [H]

Identifier: 152970049

GI number: 152970049

Start: 1664260

End: 1665282

Strand: Direct

Name: astE [H]

Synonym: KPN_01497

Alternate gene names: 152970049

Gene position: 1664260-1665282 (Clockwise)

Preceding gene: 152970048

Following gene: 152970050

Centisome position: 31.31

GC content: 65.79

Gene sequence:

>1023_bases
ATGATTTTCAGCGTTAAGGAGCGGCAGATGGAGCACCTGGTTAACGATTTACTGCACTGCCGCCTGCAGGCGTGGACCTT
TCCGGCGGGCATCGAGGCCCGCTGGCTGGGCGAGGGCATTTTACAGCTGCTGCCGACGGCGCCGTGGCGCCAGGCGACGA
TCCTCTCCGCCGGCGTGCATGGCAATGAGACCGCGCCGATTGAACTGCTGCTGCAGCTAACCCACGATCTGAGCCAGGGG
CGGCAGCCGCTCACGCAGGCGCTGCTGATCGTCTTTGGTAATCTGCCGGCGATCCGCGCCGCGCGCCGCTATCTGCATAA
CGACCTGAATCGCCTGTTCGGCGGCCGCCATCTGGCGGTCACCCCGGGAAATGAGTCGCGGCGGGCTTTTGCGCTGGAGC
AGGCGGTGCAGGCTTTTTATCGCGCCGCCGATGCGGCGGGGCCGGTCAACCGTGGTCATCTCGATATGCACACCGCCATT
CGCGGATCGCTGTATCGCCAGTTTGCGCTGCTCCCGGCCCACGCCGGGGATTTTTCGCCCGATTTCTATCAGCTGCTGCA
GGCCAGCGGGATGGACGCCGTCGTGCGCCATACCGAAGCGGGCGGCACCTTCACCCATTTCACCTGCGAAAAGTTCGCCG
CGCAAAGCGCCACCCTGGAGCTGGGGAAAGTGATGCCGTTCGGCGCCAACGATCTCAGCCTGTTCGCCGCCGCCGACGCG
GCGATCCGGGCCTGGATCGCTGACGCGCCGCTGCCGCCGCGCGACAAAGCGCCGGTGGACTACTTCCTGGTCGAGGAGAG
CATCATCAAGCGCGAGGGGGAATTTACGCTTAATCTCGCGGCAGACGTTGAAAACTTCACCGCGCTCCCGGCAGGGTATG
AGATCGCCCGCCAGGCAGAGAAACGTTGGGTGGTGCAGGCCCGGGCCCCCTATATCCTCTTTCCCAACGCCGGGGTCGCC
ACCGGCCAGCGCGCCGGGCTGTTGCTCCGCGCCGCTGCCCTTCGTCTCCCGCAACCAGCCTGA

Upstream 100 bases:

>100_bases
GACCGGCTGCATGCCCGCGATCTGGCGGACCCGCAGCTGCTGCGCGAGGTTTATCAGGCGCTGGATGAGCTGACGCAGAT
CCTGCGCCTGGGCGCGGTCT

Downstream 100 bases:

>100_bases
GGAAACACCATGACTGAACATCAACATCAGGCGTCACCTGCCGCCGGAGGTGAAGGCGCGTCACAGCTTAAGCGCGGGCT
CAGCGCCCGCCATATCCAGA

Product: succinylglutamate desuccinylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MIFSVKERQMEHLVNDLLHCRLQAWTFPAGIEARWLGEGILQLLPTAPWRQATILSAGVHGNETAPIELLLQLTHDLSQG
RQPLTQALLIVFGNLPAIRAARRYLHNDLNRLFGGRHLAVTPGNESRRAFALEQAVQAFYRAADAAGPVNRGHLDMHTAI
RGSLYRQFALLPAHAGDFSPDFYQLLQASGMDAVVRHTEAGGTFTHFTCEKFAAQSATLELGKVMPFGANDLSLFAAADA
AIRAWIADAPLPPRDKAPVDYFLVEESIIKREGEFTLNLAADVENFTALPAGYEIARQAEKRWVVQARAPYILFPNAGVA
TGQRAGLLLRAAALRLPQPA

Sequences:

>Translated_340_residues
MIFSVKERQMEHLVNDLLHCRLQAWTFPAGIEARWLGEGILQLLPTAPWRQATILSAGVHGNETAPIELLLQLTHDLSQG
RQPLTQALLIVFGNLPAIRAARRYLHNDLNRLFGGRHLAVTPGNESRRAFALEQAVQAFYRAADAAGPVNRGHLDMHTAI
RGSLYRQFALLPAHAGDFSPDFYQLLQASGMDAVVRHTEAGGTFTHFTCEKFAAQSATLELGKVMPFGANDLSLFAAADA
AIRAWIADAPLPPRDKAPVDYFLVEESIIKREGEFTLNLAADVENFTALPAGYEIARQAEKRWVVQARAPYILFPNAGVA
TGQRAGLLLRAAALRLPQPA
>Mature_340_residues
MIFSVKERQMEHLVNDLLHCRLQAWTFPAGIEARWLGEGILQLLPTAPWRQATILSAGVHGNETAPIELLLQLTHDLSQG
RQPLTQALLIVFGNLPAIRAARRYLHNDLNRLFGGRHLAVTPGNESRRAFALEQAVQAFYRAADAAGPVNRGHLDMHTAI
RGSLYRQFALLPAHAGDFSPDFYQLLQASGMDAVVRHTEAGGTFTHFTCEKFAAQSATLELGKVMPFGANDLSLFAAADA
AIRAWIADAPLPPRDKAPVDYFLVEESIIKREGEFTLNLAADVENFTALPAGYEIARQAEKRWVVQARAPYILFPNAGVA
TGQRAGLLLRAAALRLPQPA

Specific function: Transforms N(2)-succinylglutamate into succinate and glutamate [H]

COG id: COG2988

COG function: function code E; Succinylglutamate desuccinylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788040, Length=300, Percent_Identity=43.3333333333333, Blast_Score=244, Evalue=8e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007036
- InterPro:   IPR016681 [H]

Pfam domain/function: PF04952 AstE_AspA [H]

EC number: =3.5.1.96 [H]

Molecular weight: Translated: 37297; Mature: 37297

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSVKERQMEHLVNDLLHCRLQAWTFPAGIEARWLGEGILQLLPTAPWRQATILSAGVH
CCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCC
GNETAPIELLLQLTHDLSQGRQPLTQALLIVFGNLPAIRAARRYLHNDLNRLFGGRHLAV
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEE
TPGNESRRAFALEQAVQAFYRAADAAGPVNRGHLDMHTAIRGSLYRQFALLPAHAGDFSP
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCH
DFYQLLQASGMDAVVRHTEAGGTFTHFTCEKFAAQSATLELGKVMPFGANDLSLFAAADA
HHHHHHHHCCCHHHHEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AIRAWIADAPLPPRDKAPVDYFLVEESIIKREGEFTLNLAADVENFTALPAGYEIARQAE
HHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCCCEEEEEEECCHHHCCCCCCHHHHHHHH
KRWVVQARAPYILFPNAGVATGQRAGLLLRAAALRLPQPA
HHEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIFSVKERQMEHLVNDLLHCRLQAWTFPAGIEARWLGEGILQLLPTAPWRQATILSAGVH
CCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCC
GNETAPIELLLQLTHDLSQGRQPLTQALLIVFGNLPAIRAARRYLHNDLNRLFGGRHLAV
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEE
TPGNESRRAFALEQAVQAFYRAADAAGPVNRGHLDMHTAIRGSLYRQFALLPAHAGDFSP
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCH
DFYQLLQASGMDAVVRHTEAGGTFTHFTCEKFAAQSATLELGKVMPFGANDLSLFAAADA
HHHHHHHHCCCHHHHEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AIRAWIADAPLPPRDKAPVDYFLVEESIIKREGEFTLNLAADVENFTALPAGYEIARQAE
HHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCCCEEEEEEECCHHHCCCCCCHHHHHHHH
KRWVVQARAPYILFPNAGVATGQRAGLLLRAAALRLPQPA
HHEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]