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The map label for this gene is paaJ [H]

Identifier: 152970031

GI number: 152970031

Start: 1640422

End: 1641627

Strand: Direct

Name: paaJ [H]

Synonym: KPN_01478

Alternate gene names: 152970031

Gene position: 1640422-1641627 (Clockwise)

Preceding gene: 152970030

Following gene: 152970032

Centisome position: 30.86

GC content: 64.51

Gene sequence:

>1206_bases
ATGCGCGACGCCTTTATTTGTGACGGGATCCGTACGCCGATTGGCCGCTACGGCGGCGCGCTGGCCAGTGTGCGCGCCGA
CGACCTGGCGGCGATCCCGCTGCGTGAGCTCCTGAGTCGTAATCCCGGGCTCGACCCGGCAGCAATTGATGATGTGATTT
TCGGCTGTGCGAATCAGGCCGGGGAAGATAACCGCAACGTGGCGCACATGGCGACGTTGTTGGCCGGTTACCCGCATACC
GTGCCGGGCACCACGATTAACCGCCTGTGCGGATCGGGTCTGGATGCCATCGGCTTTGCCGCCAGGGCGATCAAAGCCGG
CGATGCCGATCTGCTGATTGCTGGCGGCGTGGAATCGATGTCGCGTGCGCCTTTCGTGATGGGAAAAGCCAGCGCGCCGT
ACCAGCGTCAGGCGGAACTCTTTGATACCACGATTGGCTGGCGTTTTGTGAACCCGCTCATGGCGCAACACTTCGGAACT
GACAGCATGCCGGAAACGGCCGAGAATGTAGCTGAATTGTTAAATATCAGCCGGGCCGATCAGGACGCTTTCGCCTGGCG
CAGCCAGCAGCGCACGGCGCAGGCCCAGCGCGACGGCATTCTGGCGCAGGAGATTGTGCCGGTGCAGATTGTCGGCCGGA
AAGGGGCGGTTAGCGACGTGCGCGAGGACGAGCATCCGCGCCCGGAAACCACGCTTGAGCAGCTGGCGAAACTGAAAGCG
CCGTTCCGCCAGGGCGGCGTCATTACTGCGGGCAATGCTTCCGGCGTCAACGATGGCGCCGCGGCGCTGATCGTCGCCAG
CGAACAGCAGGCCGCTATCCAGGGGCTCACGCCGCGGGCGCGTATCGTGGCGATGGCGACGGCGGGTGTCGAACCGCGCC
TGATGGGACTGGGGCCGGTACCGGCGGTGCGTAAAGTGCTGGAGCGAGCGGGACTCAATATTAACGATATGGATCTGATT
GAGCTGAATGAGGCCTTCGCCGCCCAGGCGCTGGGGGTGCTCAGACAGCTCGGCGTGCCGGACGATGCGGCGCACGTGAA
TCCCAACGGCGGCGCCATCGCGTTAGGCCATCCGCTGGGCATGAGCGGCGCGCGGTTAGCGCTGAGCGCCAGCCTTGAGC
TGCAACGCCGGGGCGGGCGCTATGCGCTGTGTACGATGTGTATTGGCGTGGGTCAGGGCATTGCCATGATCCTCGAGCGA
GTGTGA

Upstream 100 bases:

>100_bases
GCGTCTACGACATCGAAATTGTTAATCAACAGCAAAAGATTGTGGCCCTGTTCCGCGGCAAATCGCACCGCATCGGCGGC
ACAATCACGGGAGACGTGTA

Downstream 100 bases:

>100_bases
GCTAATTTACCTGCGAGTACCCTACAATGATAACTACTACAAAATTAGATCCGATTGAAACCGCATCGCGTGACGAACTG
CAGGCCCTGCAAACCCAGCG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MRDAFICDGIRTPIGRYGGALASVRADDLAAIPLRELLSRNPGLDPAAIDDVIFGCANQAGEDNRNVAHMATLLAGYPHT
VPGTTINRLCGSGLDAIGFAARAIKAGDADLLIAGGVESMSRAPFVMGKASAPYQRQAELFDTTIGWRFVNPLMAQHFGT
DSMPETAENVAELLNISRADQDAFAWRSQQRTAQAQRDGILAQEIVPVQIVGRKGAVSDVREDEHPRPETTLEQLAKLKA
PFRQGGVITAGNASGVNDGAAALIVASEQQAAIQGLTPRARIVAMATAGVEPRLMGLGPVPAVRKVLERAGLNINDMDLI
ELNEAFAAQALGVLRQLGVPDDAAHVNPNGGAIALGHPLGMSGARLALSASLELQRRGGRYALCTMCIGVGQGIAMILER
V

Sequences:

>Translated_401_residues
MRDAFICDGIRTPIGRYGGALASVRADDLAAIPLRELLSRNPGLDPAAIDDVIFGCANQAGEDNRNVAHMATLLAGYPHT
VPGTTINRLCGSGLDAIGFAARAIKAGDADLLIAGGVESMSRAPFVMGKASAPYQRQAELFDTTIGWRFVNPLMAQHFGT
DSMPETAENVAELLNISRADQDAFAWRSQQRTAQAQRDGILAQEIVPVQIVGRKGAVSDVREDEHPRPETTLEQLAKLKA
PFRQGGVITAGNASGVNDGAAALIVASEQQAAIQGLTPRARIVAMATAGVEPRLMGLGPVPAVRKVLERAGLNINDMDLI
ELNEAFAAQALGVLRQLGVPDDAAHVNPNGGAIALGHPLGMSGARLALSASLELQRRGGRYALCTMCIGVGQGIAMILER
V
>Mature_401_residues
MRDAFICDGIRTPIGRYGGALASVRADDLAAIPLRELLSRNPGLDPAAIDDVIFGCANQAGEDNRNVAHMATLLAGYPHT
VPGTTINRLCGSGLDAIGFAARAIKAGDADLLIAGGVESMSRAPFVMGKASAPYQRQAELFDTTIGWRFVNPLMAQHFGT
DSMPETAENVAELLNISRADQDAFAWRSQQRTAQAQRDGILAQEIVPVQIVGRKGAVSDVREDEHPRPETTLEQLAKLKA
PFRQGGVITAGNASGVNDGAAALIVASEQQAAIQGLTPRARIVAMATAGVEPRLMGLGPVPAVRKVLERAGLNINDMDLI
ELNEAFAAQALGVLRQLGVPDDAAHVNPNGGAIALGHPLGMSGARLALSASLELQRRGGRYALCTMCIGVGQGIAMILER
V

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=399, Percent_Identity=42.8571428571429, Blast_Score=298, Evalue=8e-81,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=40.3022670025189, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI4501853, Length=407, Percent_Identity=39.8034398034398, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI4557237, Length=402, Percent_Identity=36.0696517412935, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI4504327, Length=431, Percent_Identity=32.7146171693735, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI194353979, Length=399, Percent_Identity=29.5739348370927, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=84.788029925187, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI48994986, Length=404, Percent_Identity=44.5544554455446, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=44.9131513647643, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=43.8902743142145, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1788683, Length=424, Percent_Identity=33.2547169811321, Blast_Score=171, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI133906874, Length=404, Percent_Identity=43.0693069306931, Blast_Score=300, Evalue=7e-82,
Organism=Caenorhabditis elegans, GI25147385, Length=402, Percent_Identity=36.318407960199, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17535921, Length=408, Percent_Identity=33.8235294117647, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17551802, Length=434, Percent_Identity=30.184331797235, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17535917, Length=407, Percent_Identity=30.2211302211302, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325229, Length=411, Percent_Identity=35.5231143552311, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6322031, Length=414, Percent_Identity=36.7149758454106, Blast_Score=213, Evalue=6e-56,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=45.1371571072319, Blast_Score=324, Evalue=8e-89,
Organism=Drosophila melanogaster, GI17648125, Length=406, Percent_Identity=40.3940886699507, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24640423, Length=404, Percent_Identity=34.1584158415842, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI17137578, Length=431, Percent_Identity=30.8584686774942, Blast_Score=181, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42128; Mature: 42128

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDAFICDGIRTPIGRYGGALASVRADDLAAIPLRELLSRNPGLDPAAIDDVIFGCANQA
CCCCEEECCHHCCHHHHCCHHHHCCCCCHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCCC
GEDNRNVAHMATLLAGYPHTVPGTTINRLCGSGLDAIGFAARAIKAGDADLLIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCHHHH
SRAPFVMGKASAPYQRQAELFDTTIGWRFVNPLMAQHFGTDSMPETAENVAELLNISRAD
HCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
QDAFAWRSQQRTAQAQRDGILAQEIVPVQIVGRKGAVSDVREDEHPRPETTLEQLAKLKA
HHHHHHHHHHHHHHHHHCCCHHHHCCEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHC
PFRQGGVITAGNASGVNDGAAALIVASEQQAAIQGLTPRARIVAMATAGVEPRLMGLGPV
HHHCCCEEEECCCCCCCCCCEEEEEECCHHHHHHCCCCCCEEEEEEECCCCCEEECCCCC
PAVRKVLERAGLNINDMDLIELNEAFAAQALGVLRQLGVPDDAAHVNPNGGAIALGHPLG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCC
MSGARLALSASLELQRRGGRYALCTMCIGVGQGIAMILERV
CCCCEEEEEECHHHHHCCCCEEHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MRDAFICDGIRTPIGRYGGALASVRADDLAAIPLRELLSRNPGLDPAAIDDVIFGCANQA
CCCCEEECCHHCCHHHHCCHHHHCCCCCHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCCC
GEDNRNVAHMATLLAGYPHTVPGTTINRLCGSGLDAIGFAARAIKAGDADLLIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCHHHH
SRAPFVMGKASAPYQRQAELFDTTIGWRFVNPLMAQHFGTDSMPETAENVAELLNISRAD
HCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
QDAFAWRSQQRTAQAQRDGILAQEIVPVQIVGRKGAVSDVREDEHPRPETTLEQLAKLKA
HHHHHHHHHHHHHHHHHCCCHHHHCCEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHC
PFRQGGVITAGNASGVNDGAAALIVASEQQAAIQGLTPRARIVAMATAGVEPRLMGLGPV
HHHCCCEEEECCCCCCCCCCEEEEEECCHHHHHHCCCCCCEEEEEEECCCCCEEECCCCC
PAVRKVLERAGLNINDMDLIELNEAFAAQALGVLRQLGVPDDAAHVNPNGGAIALGHPLG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCC
MSGARLALSASLELQRRGGRYALCTMCIGVGQGIAMILERV
CCCCEEEEEECHHHHHCCCCEEHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]