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The map label for this gene is paaI [H]

Identifier: 152970030

GI number: 152970030

Start: 1640000

End: 1640422

Strand: Direct

Name: paaI [H]

Synonym: KPN_01477

Alternate gene names: 152970030

Gene position: 1640000-1640422 (Clockwise)

Preceding gene: 152970029

Following gene: 152970031

Centisome position: 30.86

GC content: 56.97

Gene sequence:

>423_bases
ATGAGCAATGATCCGTGGCGCAACGCGCGCGTGATGTACGAAAAAGATACCTGCGCCAGAAAGATGGGGATCGAACTTAT
CGAAATGGACGACGGTTTTGCGCAGATGAGTATGACCGTCTCGGCGGACATGCTTAACGGCCATCAAACCTGTCACGGTG
GTCAGCTATTTACGCTTGCCGATACCGCTTTCGCCTACGCCTGTAACAGCCAGGGGCTGGCGGCGGTGGCCTCGGCGGCA
AGCATTGATTTTCTTCGTCCGGCGTTTGCCGGCGACCGGCTGGTCGCCACCGCCAGAGTCAAACAGCAGGGCAAGCTGAC
CGGCGTCTACGACATCGAAATTGTTAATCAACAGCAAAAGATTGTGGCCCTGTTCCGCGGCAAATCGCACCGCATCGGCG
GCACAATCACGGGAGACGTGTAA

Upstream 100 bases:

>100_bases
CGGGTGCTGCAACTGCTGGAGAACCTGCAACACCATTATGGCGAGGAGCGCTATCGCCCCAGTTCCCTGCTGCGCCAGAA
GGCGCTGATGGAGAAGCACC

Downstream 100 bases:

>100_bases
TGCGCGACGCCTTTATTTGTGACGGGATCCGTACGCCGATTGGCCGCTACGGCGGCGCGCTGGCCAGTGTGCGCGCCGAC
GACCTGGCGGCGATCCCGCT

Product: phenylacetic acid degradation protein

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MSNDPWRNARVMYEKDTCARKMGIELIEMDDGFAQMSMTVSADMLNGHQTCHGGQLFTLADTAFAYACNSQGLAAVASAA
SIDFLRPAFAGDRLVATARVKQQGKLTGVYDIEIVNQQQKIVALFRGKSHRIGGTITGDV

Sequences:

>Translated_140_residues
MSNDPWRNARVMYEKDTCARKMGIELIEMDDGFAQMSMTVSADMLNGHQTCHGGQLFTLADTAFAYACNSQGLAAVASAA
SIDFLRPAFAGDRLVATARVKQQGKLTGVYDIEIVNQQQKIVALFRGKSHRIGGTITGDV
>Mature_139_residues
SNDPWRNARVMYEKDTCARKMGIELIEMDDGFAQMSMTVSADMLNGHQTCHGGQLFTLADTAFAYACNSQGLAAVASAAS
IDFLRPAFAGDRLVATARVKQQGKLTGVYDIEIVNQQQKIVALFRGKSHRIGGTITGDV

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=139, Percent_Identity=76.2589928057554, Blast_Score=231, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 15177; Mature: 15045

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDPWRNARVMYEKDTCARKMGIELIEMDDGFAQMSMTVSADMLNGHQTCHGGQLFTLA
CCCCCCCCCEEEEECCHHHHHHCCEEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEEEE
DTAFAYACNSQGLAAVASAASIDFLRPAFAGDRLVATARVKQQGKLTGVYDIEIVNQQQK
CCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEHHCCCCEEEEEEEEEECCCCE
IVALFRGKSHRIGGTITGDV
EEEEECCCCCCCCEEEECCC
>Mature Secondary Structure 
SNDPWRNARVMYEKDTCARKMGIELIEMDDGFAQMSMTVSADMLNGHQTCHGGQLFTLA
CCCCCCCCEEEEECCHHHHHHCCEEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEEEE
DTAFAYACNSQGLAAVASAASIDFLRPAFAGDRLVATARVKQQGKLTGVYDIEIVNQQQK
CCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEHHCCCCEEEEEEEEEECCCCE
IVALFRGKSHRIGGTITGDV
EEEEECCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]