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The map label for this gene is ycjG [H]

Identifier: 152969861

GI number: 152969861

Start: 1467966

End: 1468931

Strand: Direct

Name: ycjG [H]

Synonym: KPN_01308

Alternate gene names: 152969861

Gene position: 1467966-1468931 (Clockwise)

Preceding gene: 152969860

Following gene: 152969863

Centisome position: 27.62

GC content: 65.84

Gene sequence:

>966_bases
ATGCGTAACGTGCGAGTGTATGAGGAAGCCTGGCCGCTCCATACCCCTTTTGTGATCGCCCGCGGTAGCCGCAGCGAGGC
AAAAGTGGTGGTAGTGGAACTGGAAGAGGACGGCGTGAAAGGGGTAGGCGAATGCACACCCTATCCCCGCTATGGCGAGA
GCATCGCCTCGGTCATGGCCCAGGTGATGGCGATCGGCGAGCAACTGGAGGCGGGGCTTACCCGCGAACAGCTGCAGCGG
CTGCTGCCGGCAGGCGCCGCCCGCAATGCCATTGACTGCGCGCTATGGGATCTGCAGGCCCGGCGCGAAGGCAAAACCTT
GGCCCAGCTGTTGGGCGTGGCGTTGCCTAACCGGGTGATTACCGCCCAGACGGTGGTCATTGGCACGCCGGATCAGATGG
CGGCCAGCGCCGCGGCGCTGTGGCAGGCAGGGGCCCAGCTGTTGAAGGTGAAGCTCGACGATCGACTGATCAGCGAGCGG
CTAATCGCTATCCGCCAGGCGGCGCCGGAGGCTACGCTGATTGTTGACGCCAATGAGTCCTGGCACAGCGAGGGGCTGGC
GGCGCGCTGTCAGCTGCTGGCGGACCTCGGTGTGGCGATGCTTGAGCAGCCGCTCCCCGCCGACGACGACTCGGCGCTGG
AAAACTTCGTTCATCCGCTGCCGATTTGCGCCGACGAGAGCTGCCACACCCGGGAGAGCCTGCCGCGGCTGCGCGGGCGC
TATGAGATGATCAATATCAAACTGGATAAAACGGGCGGCCTGACCGAAGCGCTGGCTCTGGCCGGGGAGGCGGAACGGCA
GGGATTTGAACGGATGCTGGGCTGTATGCTTTGCACCTCGCGCGGGATCGCCGCCGCGCTCCCTCTGGCGCCGCTGGCGC
GCTTCGCTGACCTCGACGGCCCGACATGGCTGGCGGTGGACGTTGAGCCGGCGCTGCGGTTCAGCACCGGCGTGCTTCAT
CTTTAA

Upstream 100 bases:

>100_bases
TTATCTTCCTGTTTACATATAGTTAACGTCGCGAGTAGTGTATGACATCCGCCAGCTACACGGCAAACCCTATTTAACAT
TTACCAGAGAGGACAAGGGA

Downstream 100 bases:

>100_bases
GCCTGCCAGCGCAGCAGATCGGTCATCGCCGGCAGGTAACGCTCCGTTGCCTCGTCTGCGGAGACCGGCGGAAACTCGGC
GGTGATGCAGGGGAGTCCGA

Product: L-Ala-D/L-Glu epimerase, a muconate lactonizing enzyme

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRNVRVYEEAWPLHTPFVIARGSRSEAKVVVVELEEDGVKGVGECTPYPRYGESIASVMAQVMAIGEQLEAGLTREQLQR
LLPAGAARNAIDCALWDLQARREGKTLAQLLGVALPNRVITAQTVVIGTPDQMAASAAALWQAGAQLLKVKLDDRLISER
LIAIRQAAPEATLIVDANESWHSEGLAARCQLLADLGVAMLEQPLPADDDSALENFVHPLPICADESCHTRESLPRLRGR
YEMINIKLDKTGGLTEALALAGEAERQGFERMLGCMLCTSRGIAAALPLAPLARFADLDGPTWLAVDVEPALRFSTGVLH
L

Sequences:

>Translated_321_residues
MRNVRVYEEAWPLHTPFVIARGSRSEAKVVVVELEEDGVKGVGECTPYPRYGESIASVMAQVMAIGEQLEAGLTREQLQR
LLPAGAARNAIDCALWDLQARREGKTLAQLLGVALPNRVITAQTVVIGTPDQMAASAAALWQAGAQLLKVKLDDRLISER
LIAIRQAAPEATLIVDANESWHSEGLAARCQLLADLGVAMLEQPLPADDDSALENFVHPLPICADESCHTRESLPRLRGR
YEMINIKLDKTGGLTEALALAGEAERQGFERMLGCMLCTSRGIAAALPLAPLARFADLDGPTWLAVDVEPALRFSTGVLH
L
>Mature_321_residues
MRNVRVYEEAWPLHTPFVIARGSRSEAKVVVVELEEDGVKGVGECTPYPRYGESIASVMAQVMAIGEQLEAGLTREQLQR
LLPAGAARNAIDCALWDLQARREGKTLAQLLGVALPNRVITAQTVVIGTPDQMAASAAALWQAGAQLLKVKLDDRLISER
LIAIRQAAPEATLIVDANESWHSEGLAARCQLLADLGVAMLEQPLPADDDSALENFVHPLPICADESCHTRESLPRLRGR
YEMINIKLDKTGGLTEALALAGEAERQGFERMLGCMLCTSRGIAAALPLAPLARFADLDGPTWLAVDVEPALRFSTGVLH
L

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=321, Percent_Identity=79.7507788161994, Blast_Score=519, Evalue=1e-149,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 34713; Mature: 34713

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNVRVYEEAWPLHTPFVIARGSRSEAKVVVVELEEDGVKGVGECTPYPRYGESIASVMA
CCCCEEEHHCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
QVMAIGEQLEAGLTREQLQRLLPAGAARNAIDCALWDLQARREGKTLAQLLGVALPNRVI
HHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEE
TAQTVVIGTPDQMAASAAALWQAGAQLLKVKLDDRLISERLIAIRQAAPEATLIVDANES
EEEEEEEECCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
WHSEGLAARCQLLADLGVAMLEQPLPADDDSALENFVHPLPICADESCHTRESLPRLRGR
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCC
YEMINIKLDKTGGLTEALALAGEAERQGFERMLGCMLCTSRGIAAALPLAPLARFADLDG
EEEEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHCCCC
PTWLAVDVEPALRFSTGVLHL
CEEEEEECCHHHHHCCCCCCC
>Mature Secondary Structure
MRNVRVYEEAWPLHTPFVIARGSRSEAKVVVVELEEDGVKGVGECTPYPRYGESIASVMA
CCCCEEEHHCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
QVMAIGEQLEAGLTREQLQRLLPAGAARNAIDCALWDLQARREGKTLAQLLGVALPNRVI
HHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEE
TAQTVVIGTPDQMAASAAALWQAGAQLLKVKLDDRLISERLIAIRQAAPEATLIVDANES
EEEEEEEECCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
WHSEGLAARCQLLADLGVAMLEQPLPADDDSALENFVHPLPICADESCHTRESLPRLRGR
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCC
YEMINIKLDKTGGLTEALALAGEAERQGFERMLGCMLCTSRGIAAALPLAPLARFADLDG
EEEEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHCCCC
PTWLAVDVEPALRFSTGVLHL
CEEEEEECCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]