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The map label for this gene is yciL [H]

Identifier: 152969819

GI number: 152969819

Start: 1419724

End: 1420626

Strand: Direct

Name: yciL [H]

Synonym: KPN_01265

Alternate gene names: 152969819

Gene position: 1419724-1420626 (Clockwise)

Preceding gene: 152969814

Following gene: 152969822

Centisome position: 26.71

GC content: 59.47

Gene sequence:

>903_bases
ATGAGCGAGAAACTGCAAAAAGTGCTGGCCCGCGCCGGCCACGGCTCCCGCCGTGAAATTGAAGCGAAAATCGAAGCTGG
CCGCGTCAGCGTCGACGGCAAAATCGCCACCCTCGGCGATCGTGTGGAAATTGTCCCTGGTTTAAAAATTCGTATCGATG
GTCATCTGATCTCGGTGAAAGAGTCTGCCGAGCAGATCTGCCGCGTGCTGGCTTACTATAAGCCGGAAGGCGAGCTGTGC
ACCCGTAACGATCCGGAAGGACGCCCGACGGTATTTGATCGTCTGCCTAAACTGCGCGGCGCGCGCTGGATTGCCGTTGG
TCGTCTGGACGTGAATACCTGCGGCCTGCTGCTGTTCACCACCGATGGCGAACTGGCCAACCGCCTGATGCACCCGAGCC
GTGAAGTCGAGCGTGAGTACGCCGTTCGCGTTTTCGGCCAGGTGGATGATGATAAGCTGCGCCAGCTGTCGCGCGGCGTA
CAGTTAGAAGATGGTCCGGCAGCGTTTAAAACCATCAAATTCACCGGCGGCGAAGGGATTAACCAGTGGTATAACGTTAC
CCTGACCGAAGGGCGTAACCGCGAAGTGCGTCGCCTGTGGGAAGCGGTCGGCGTGCAGGTCAGCCGTCTGATCCGCGTGC
GCTACGGCGATATTCTGCTGCCGAAAGGCCTGCCGCGCGGCGGCTATACCGAGCTGGACCTGGCGCAGACCAACTACCTG
CGCGAGCTGGTGGAGCTCACGCCGGAAACCAGCTCTAAGGTTGCCGTCGAGAAAGACCGTCGGCGGTTGAAAGCCAATCA
GATCCGTCGGGCGGTGAAGCGTCACAGCCAGACCAGCGCTAATCCGCGCCAGGGTAACAACAGCAACCGTCGTTCCAGTA
CTCGCAATAAGACTAACGGTTAA

Upstream 100 bases:

>100_bases
AAATATTTACAGATCAGTGAGTTAAGTTGAGTTTTAGGCTAACTCGTTGTTTTTGAAAGCATACCAGACGCCTGGGAAGG
CGACACCTTAAGGAAGCTCA

Downstream 100 bases:

>100_bases
TGTTATGCGTTCCGGTGACTTGCCGCCGGAACGCTTACTTCCTTCCGCGCTATCAGGCCCTCAGTAGTCTATCCCCATCT
GCGCTTTAATCCCGGCATCA

Product: 23S rRNA pseudouridylate synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSEKLQKVLARAGHGSRREIEAKIEAGRVSVDGKIATLGDRVEIVPGLKIRIDGHLISVKESAEQICRVLAYYKPEGELC
TRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDDKLRQLSRGV
QLEDGPAAFKTIKFTGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDILLPKGLPRGGYTELDLAQTNYL
RELVELTPETSSKVAVEKDRRRLKANQIRRAVKRHSQTSANPRQGNNSNRRSSTRNKTNG

Sequences:

>Translated_300_residues
MSEKLQKVLARAGHGSRREIEAKIEAGRVSVDGKIATLGDRVEIVPGLKIRIDGHLISVKESAEQICRVLAYYKPEGELC
TRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDDKLRQLSRGV
QLEDGPAAFKTIKFTGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDILLPKGLPRGGYTELDLAQTNYL
RELVELTPETSSKVAVEKDRRRLKANQIRRAVKRHSQTSANPRQGNNSNRRSSTRNKTNG
>Mature_299_residues
SEKLQKVLARAGHGSRREIEAKIEAGRVSVDGKIATLGDRVEIVPGLKIRIDGHLISVKESAEQICRVLAYYKPEGELCT
RNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDDKLRQLSRGVQ
LEDGPAAFKTIKFTGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDILLPKGLPRGGYTELDLAQTNYLR
ELVELTPETSSKVAVEKDRRRLKANQIRRAVKRHSQTSANPRQGNNSNRRSSTRNKTNG

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=289, Percent_Identity=92.7335640138408, Blast_Score=542, Evalue=1e-155,
Organism=Escherichia coli, GI1790453, Length=310, Percent_Identity=29.0322580645161, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=32.0512820512821, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=33.7016574585635, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 33848; Mature: 33717

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARAGHGSRREIEAKIEAGRVSVDGKIATLGDRVEIVPGLKIRIDGHLISVK
CCHHHHHHHHHCCCCCCCEEEEEEECCEEEECCEEEECCCEEEECCCEEEEECCEEEEHH
ESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCEEEEEE
TDGELANRLMHPSREVEREYAVRVFGQVDDDKLRQLSRGVQLEDGPAAFKTIKFTGGEGI
CCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCC
NQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDILLPKGLPRGGYTELDLAQTNYL
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEHHHHHHHH
RELVELTPETSSKVAVEKDRRRLKANQIRRAVKRHSQTSANPRQGNNSNRRSSTRNKTNG
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEKLQKVLARAGHGSRREIEAKIEAGRVSVDGKIATLGDRVEIVPGLKIRIDGHLISVK
CHHHHHHHHHCCCCCCCEEEEEEECCEEEECCEEEECCCEEEECCCEEEEECCEEEEHH
ESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCEEEEEE
TDGELANRLMHPSREVEREYAVRVFGQVDDDKLRQLSRGVQLEDGPAAFKTIKFTGGEGI
CCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCC
NQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDILLPKGLPRGGYTELDLAQTNYL
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEHHHHHHHH
RELVELTPETSSKVAVEKDRRRLKANQIRRAVKRHSQTSANPRQGNNSNRRSSTRNKTNG
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]