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The map label for this gene is gcvA [H]

Identifier: 152969814

GI number: 152969814

Start: 1415529

End: 1416425

Strand: Direct

Name: gcvA [H]

Synonym: KPN_01260

Alternate gene names: 152969814

Gene position: 1415529-1416425 (Clockwise)

Preceding gene: 152969813

Following gene: 152969819

Centisome position: 26.63

GC content: 63.32

Gene sequence:

>897_bases
ATGAAACTCCCGCCGCTGAATGCGTTGCGCTGCTTTGAGGCGGCGGCGCGGCTGCTCAGCCTGAAGCTGGCCGCCAGTGA
GCTTTGCGTCACGCCCAGCGCCGTCAGCCAGCAGATTGCCCGCCTTGAGGAGACGCTGAATGTCCCGCTATTTATCCGTA
CTCCGCGACGGTTACAGTTAACGGCGGTGGGAGAGATCTACCTGCGGGCCATTCAGCCGGCGTTTCATCAGATAGCCGCC
GCCACCCAGCGTCTGCAGGTGCCCCCGGGACCAGAAAAGGTAGCTATCAGCTGTACCAGCGGCTTCGCCATTCAGTGGCT
GCTGCCGCGGCTGGCTGATTTTGAACAACGCTATCCGCAGATAGAGATCCAGATCGGCACCACCAACCGGCGAGTGGATT
TGCTGAGTGAAGGGATCGATTTTGCCGTGCGTCATGGTGTCGGCGGCTGGCCGGAACTGCAGGCTTACCGCCTGCTGGAT
GACGACCTGATGCCGGTCTGTAGCCCGCAACTGATTGCTCCGCGCCGGACGCTCACCGATGCGAGCGATCTACTGCAGTA
TCCGCTGCTGCACGATGAACATCGTGAAGACTGGGCGCTGTGGTGTGAGGCCGTGGGGCTGGCGCCGCACTTCGCGCGGC
GTGGGCCGGTGTTCACTGATTCCAACGGCGTGACGGAGGCGGCGTTCGCCGGTATGGGGATGGCATTGCTGCGACGCTCA
TTCATAGCGCCTGCGCTGACGCAGGGGCGGCTGGTTAATCCTCTGGCGCAGCACATCGCCTGTCCGCTGGCCTATCATCT
GGTTTACCACGAGACCGCGCTGCTGGCGCCTGCCAACCGCTGTTTTCGCGACTGGCTGCTTGGCCAGCGCCCGGCCGTTG
CTGAGGAGCACGCGTAG

Upstream 100 bases:

>100_bases
TGTAGTCACGAAGGAAGTCAACTGTAGAACCCACGTGCAGCGTGCGACAAACGCGTTATGCTTACATGATTAAGTTTTTC
TTAACCGAGGTTCCATACCC

Downstream 100 bases:

>100_bases
TGAGGGGGGCGCTGTCCGATAAAGATCCGTATGCGTGAATGGGCACAGGAGAATGAGGAAAATCTGTTCCATACTAAAGA
CGGGGCGCGGCGAACAGAAG

Product: transcriptional regulator LysR

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKLPPLNALRCFEAAARLLSLKLAASELCVTPSAVSQQIARLEETLNVPLFIRTPRRLQLTAVGEIYLRAIQPAFHQIAA
ATQRLQVPPGPEKVAISCTSGFAIQWLLPRLADFEQRYPQIEIQIGTTNRRVDLLSEGIDFAVRHGVGGWPELQAYRLLD
DDLMPVCSPQLIAPRRTLTDASDLLQYPLLHDEHREDWALWCEAVGLAPHFARRGPVFTDSNGVTEAAFAGMGMALLRRS
FIAPALTQGRLVNPLAQHIACPLAYHLVYHETALLAPANRCFRDWLLGQRPAVAEEHA

Sequences:

>Translated_298_residues
MKLPPLNALRCFEAAARLLSLKLAASELCVTPSAVSQQIARLEETLNVPLFIRTPRRLQLTAVGEIYLRAIQPAFHQIAA
ATQRLQVPPGPEKVAISCTSGFAIQWLLPRLADFEQRYPQIEIQIGTTNRRVDLLSEGIDFAVRHGVGGWPELQAYRLLD
DDLMPVCSPQLIAPRRTLTDASDLLQYPLLHDEHREDWALWCEAVGLAPHFARRGPVFTDSNGVTEAAFAGMGMALLRRS
FIAPALTQGRLVNPLAQHIACPLAYHLVYHETALLAPANRCFRDWLLGQRPAVAEEHA
>Mature_298_residues
MKLPPLNALRCFEAAARLLSLKLAASELCVTPSAVSQQIARLEETLNVPLFIRTPRRLQLTAVGEIYLRAIQPAFHQIAA
ATQRLQVPPGPEKVAISCTSGFAIQWLLPRLADFEQRYPQIEIQIGTTNRRVDLLSEGIDFAVRHGVGGWPELQAYRLLD
DDLMPVCSPQLIAPRRTLTDASDLLQYPLLHDEHREDWALWCEAVGLAPHFARRGPVFTDSNGVTEAAFAGMGMALLRRS
FIAPALTQGRLVNPLAQHIACPLAYHLVYHETALLAPANRCFRDWLLGQRPAVAEEHA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=38.9830508474576, Blast_Score=210, Evalue=9e-56,
Organism=Escherichia coli, GI1788706, Length=277, Percent_Identity=31.0469314079422, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=32.7586206896552, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI87081978, Length=264, Percent_Identity=32.1969696969697, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=27.0547945205479, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786401, Length=273, Percent_Identity=25.6410256410256, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI157672245, Length=259, Percent_Identity=28.957528957529, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787589, Length=280, Percent_Identity=27.1428571428571, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787128, Length=308, Percent_Identity=24.3506493506494, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787806, Length=252, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790262, Length=120, Percent_Identity=35.8333333333333, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789440, Length=249, Percent_Identity=24.8995983935743, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789639, Length=145, Percent_Identity=27.5862068965517, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=28.2828282828283, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33099; Mature: 33099

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPPLNALRCFEAAARLLSLKLAASELCVTPSAVSQQIARLEETLNVPLFIRTPRRLQL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCEEEE
TAVGEIYLRAIQPAFHQIAAATQRLQVPPGPEKVAISCTSGFAIQWLLPRLADFEQRYPQ
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCE
IEIQIGTTNRRVDLLSEGIDFAVRHGVGGWPELQAYRLLDDDLMPVCSPQLIAPRRTLTD
EEEEECCCCCHHHHHHHCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCC
ASDLLQYPLLHDEHREDWALWCEAVGLAPHFARRGPVFTDSNGVTEAAFAGMGMALLRRS
HHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHH
FIAPALTQGRLVNPLAQHIACPLAYHLVYHETALLAPANRCFRDWLLGQRPAVAEEHA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKLPPLNALRCFEAAARLLSLKLAASELCVTPSAVSQQIARLEETLNVPLFIRTPRRLQL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCEEEE
TAVGEIYLRAIQPAFHQIAAATQRLQVPPGPEKVAISCTSGFAIQWLLPRLADFEQRYPQ
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCE
IEIQIGTTNRRVDLLSEGIDFAVRHGVGGWPELQAYRLLDDDLMPVCSPQLIAPRRTLTD
EEEEECCCCCHHHHHHHCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCC
ASDLLQYPLLHDEHREDWALWCEAVGLAPHFARRGPVFTDSNGVTEAAFAGMGMALLRRS
HHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHH
FIAPALTQGRLVNPLAQHIACPLAYHLVYHETALLAPANRCFRDWLLGQRPAVAEEHA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]