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The map label for this gene is ubiF [H]

Identifier: 152969258

GI number: 152969258

Start: 777355

End: 778530

Strand: Direct

Name: ubiF [H]

Synonym: KPN_00687

Alternate gene names: 152969258

Gene position: 777355-778530 (Clockwise)

Preceding gene: 152969246

Following gene: 152969264

Centisome position: 14.63

GC content: 65.73

Gene sequence:

>1176_bases
ATGACAATTCACGTAACAGATGTCGCCATCGTGGGCGGCGGCATGGTCGGCGGCGCCCTGGCGTTAGGGCTGGCGCAACA
GGGATTTACCGTGACGGTGCTTGAGAAAGCGGCGCCACCGGCGTTCGATCCGGCATCCGCCCCGGATGTGCGGATCTCGG
CGATCAGCGCGGCTTCGGTCGGCCTGCTGAAGAGTCTCGGCGTCTGGGATGCCATACGGGCGATGCGCGTCCATGCTTAT
CGCCGTCTGGAGACCTGGGAGTGGGAGAGCGCGCACGTCGCCTTCGACGCCGCGGAGCTCAAGCTGCCGGAGCTGGGCTA
TATGGTCGAGAACAAGGTTCTGCAATGGGGCCTGTGGCAAGCGCTGGCGGCGCATGAGGCGGTTACCCTGCGCGTCGGTA
GCGAACTGAAAACGATGCAGCGCGGCGAGACGCAGACGGCGTTGCATTTGCGCGAGGGGGAGACGATCCACGCCCGGCTG
GTTATCGGCGCCGACGGCGCTAACTCCCAGGTGCGGGAGATGGCCGGCATCGGCGTGCACGCCTGGCAATATCAGCAGTC
CTGTATGCTGATCAGCGTCGAATGCGCCGACGATCCCGGCGACAGCACCTGGCAGCAGTTTACTCCCAGCGGCCCGCGCG
CCTTCCTGCCGCTGTTCGACCACTGGGCCTCGCTGGTCTGGTACGACGCCCCGGCGCGGATCCGTCAGCTGCAGAGCATG
ACCATGGCGCAGCTCCAGCAGGAGATCGCCAGTCACTTTCCGGCGCGGCTGGGACGGGTGACGCCGCAGGCGGCCGGCGC
CTTCCCGCTCACCCGGCGTCATGCGCTGCAGTATGTTCAGCCCGGCCTGGCGCTGGTGGGCGATGCGGCGCACACCATTC
ATCCACTGGCGGGGCAGGGGGTTAACCTTGGCTATCGTGATGTCGATGCCCTGCTGGAGATCCTGGCGGAAGTGCGCGGC
CGCGGCGAAGACTGGGCCAGCCTGCCGGTGCTGAAGCGCTACCAGGCGCGGCGGCGAGCGGATAATTTTATCATGCAGTC
GGGGATGGACCTCTTTTATGCCGGTTTCAGCAATGACCTGGCGCCGGTGCGGATGCTGCGTAATATTGGCCTGATGGCGG
CTGAACGCGCCGGGGTGCTGAAGCGTCAGGCGCTGAAGTATGCCTTAGGCTTGTAA

Upstream 100 bases:

>100_bases
TGTAATGCTTTGGTCGCCCCCTGACCAGTGCGAGCGTTATCGCCGCAAGAGGGCGAAAAATCCGGTAAACTTAACGGATT
CTGCTTTAAGGATAATAGCC

Downstream 100 bases:

>100_bases
AACCGGCTTCCCGGGCAGGGGCGTAGCGGAGAGCGCCTGCCCGGCAGTCAGTACGGAGTAAGCATGTCAGGACGCCAATA
GCAAAAAAGCCCACCGAAGT

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTIHVTDVAIVGGGMVGGALALGLAQQGFTVTVLEKAAPPAFDPASAPDVRISAISAASVGLLKSLGVWDAIRAMRVHAY
RRLETWEWESAHVAFDAAELKLPELGYMVENKVLQWGLWQALAAHEAVTLRVGSELKTMQRGETQTALHLREGETIHARL
VIGADGANSQVREMAGIGVHAWQYQQSCMLISVECADDPGDSTWQQFTPSGPRAFLPLFDHWASLVWYDAPARIRQLQSM
TMAQLQQEIASHFPARLGRVTPQAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALLEILAEVRG
RGEDWASLPVLKRYQARRRADNFIMQSGMDLFYAGFSNDLAPVRMLRNIGLMAAERAGVLKRQALKYALGL

Sequences:

>Translated_391_residues
MTIHVTDVAIVGGGMVGGALALGLAQQGFTVTVLEKAAPPAFDPASAPDVRISAISAASVGLLKSLGVWDAIRAMRVHAY
RRLETWEWESAHVAFDAAELKLPELGYMVENKVLQWGLWQALAAHEAVTLRVGSELKTMQRGETQTALHLREGETIHARL
VIGADGANSQVREMAGIGVHAWQYQQSCMLISVECADDPGDSTWQQFTPSGPRAFLPLFDHWASLVWYDAPARIRQLQSM
TMAQLQQEIASHFPARLGRVTPQAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALLEILAEVRG
RGEDWASLPVLKRYQARRRADNFIMQSGMDLFYAGFSNDLAPVRMLRNIGLMAAERAGVLKRQALKYALGL
>Mature_390_residues
TIHVTDVAIVGGGMVGGALALGLAQQGFTVTVLEKAAPPAFDPASAPDVRISAISAASVGLLKSLGVWDAIRAMRVHAYR
RLETWEWESAHVAFDAAELKLPELGYMVENKVLQWGLWQALAAHEAVTLRVGSELKTMQRGETQTALHLREGETIHARLV
IGADGANSQVREMAGIGVHAWQYQQSCMLISVECADDPGDSTWQQFTPSGPRAFLPLFDHWASLVWYDAPARIRQLQSMT
MAQLQQEIASHFPARLGRVTPQAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALLEILAEVRGR
GEDWASLPVLKRYQARRRADNFIMQSGMDLFYAGFSNDLAPVRMLRNIGLMAAERAGVLKRQALKYALGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=386, Percent_Identity=32.9015544041451, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI32967307, Length=345, Percent_Identity=31.5942028985507, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1786883, Length=391, Percent_Identity=78.772378516624, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI1789273, Length=390, Percent_Identity=40, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1789274, Length=383, Percent_Identity=33.1592689295039, Blast_Score=171, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI25146631, Length=430, Percent_Identity=28.8372093023256, Blast_Score=193, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321694, Length=453, Percent_Identity=25.60706401766, Blast_Score=122, Evalue=9e-29,
Organism=Drosophila melanogaster, GI19920760, Length=420, Percent_Identity=28.0952380952381, Blast_Score=164, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 42701; Mature: 42569

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIHVTDVAIVGGGMVGGALALGLAQQGFTVTVLEKAAPPAFDPASAPDVRISAISAASV
CEEEEEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEEEHHHHH
GLLKSLGVWDAIRAMRVHAYRRLETWEWESAHVAFDAAELKLPELGYMVENKVLQWGLWQ
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHH
ALAAHEAVTLRVGSELKTMQRGETQTALHLREGETIHARLVIGADGANSQVREMAGIGVH
HHHHCCEEEEEECHHHHHHHCCCCHHEEEEECCCEEEEEEEEECCCCCHHHHHHHCCCCC
AWQYQQSCMLISVECADDPGDSTWQQFTPSGPRAFLPLFDHWASLVWYDAPARIRQLQSM
CEEECCCEEEEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHEEECCHHHHHHHHHH
TMAQLQQEIASHFPARLGRVTPQAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHEECCCHHHHCCCCCCC
VNLGYRDVDALLEILAEVRGRGEDWASLPVLKRYQARRRADNFIMQSGMDLFYAGFSNDL
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCH
APVRMLRNIGLMAAERAGVLKRQALKYALGL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIHVTDVAIVGGGMVGGALALGLAQQGFTVTVLEKAAPPAFDPASAPDVRISAISAASV
EEEEEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEEEHHHHH
GLLKSLGVWDAIRAMRVHAYRRLETWEWESAHVAFDAAELKLPELGYMVENKVLQWGLWQ
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHH
ALAAHEAVTLRVGSELKTMQRGETQTALHLREGETIHARLVIGADGANSQVREMAGIGVH
HHHHCCEEEEEECHHHHHHHCCCCHHEEEEECCCEEEEEEEEECCCCCHHHHHHHCCCCC
AWQYQQSCMLISVECADDPGDSTWQQFTPSGPRAFLPLFDHWASLVWYDAPARIRQLQSM
CEEECCCEEEEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHEEECCHHHHHHHHHH
TMAQLQQEIASHFPARLGRVTPQAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHEECCCHHHHCCCCCCC
VNLGYRDVDALLEILAEVRGRGEDWASLPVLKRYQARRRADNFIMQSGMDLFYAGFSNDL
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCH
APVRMLRNIGLMAAERAGVLKRQALKYALGL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]