| Definition | Klebsiella pneumoniae subsp. pneumoniae MGH 78578 chromosome, complete genome. |
|---|---|
| Accession | NC_009648 |
| Length | 5,315,120 |
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The map label for this gene is sfmD [H]
Identifier: 152968851
GI number: 152968851
Start: 312969
End: 315452
Strand: Direct
Name: sfmD [H]
Synonym: KPN_00277
Alternate gene names: 152968851
Gene position: 312969-315452 (Clockwise)
Preceding gene: 152968850
Following gene: 152968852
Centisome position: 5.89
GC content: 52.98
Gene sequence:
>2484_bases ATGATGTCTGCGCTATTCAATGCTCCAGCCATATCGTTTGCGGATGAGTTTTTTAATCCTGCTTTGCTTGAGGTTCGCGA AGGAGAAGGTGCGCCGACGACAGACCTCTCCGTATTTGAAGTCGTCAATGGACAGCCAGCAGGAAACTACCGGGTAGATA TCTACGTCAATAACGAAATGGTGACCGCAGATGCCCGCGATGTGATGTTTTCAAAGAACCAGGATGGGAAGCTGGAGCCC TGCGTCACGTTGGCAGAACTCAGCCAATGGGGAGTTGATACGGCCTCATTTCCAGAACTCGCCGTCAGTGATAGCGAATG CGCCAATCTTGCGGGCATCCCTGATGCATTTGCCGACTTCCAGTTTAACCGCCAAAAGCTGAATCTGAGTATCCCTCATG CGGCAATGAATACGCGCAGCCTGGATGAAGTGCCTATTGAACGTTGGGATGAAGGTATTCCAGCCCTTCTCCTCAATTAC AACATGAATAGCTATCAGAGCCGATATAAGGGTGCAGAAACTTACGAAAGCACGTATTTGAACCTGCGTCCCGGTCTCAA TATCGGTCCCTGGCGTCTGCGCAACTACACCACCTTTGAGCATAGTAATCAGAGTGGAAGTAAATGGGACAACGTCTATA GCTATCTTCAGCGGAATATTGTCTCTTTGCGAAGCCAACTGACGTTAGGCGACAGTTCAGCACCAGCTGATGTCTTCGAT AGCGTGCCGTTTCGTGGTTTTCAGATGGCCTCGGATGATGACATGCTGCCTTCCAGCGTACGTAACTATGCGCCTGTGAT TCGAGGGGTTGCCCGCAGCAATGCCCTGGTCACCATCCGTCAGAATGGTTACGTTGTCTATGAGACCAAAGTGGCGCCGG GGGCGTTCGAGATCTCCGATTTGGCCTCCACCGGAGGAGCAGGCGATCTTGACGTGACCATCAAAGAAGCCGACGGCAGC GAGCAGCACTTGCGCATCCCCTACGCATCTTTGCCTGTTCTACAGCGTGAAGGTAAGTTTCGCTATAGCGTTACGGGCGG TCAATTCCGCGCCTACGATTCTCACACGGAAAAAAACCTGTTTGTTCAGGGCACCGGAATTTATGGCCTTCCGTGGGGCT TTACGCTCTACGGCGGCGTGCAAAATGCAGGCAATAAATATCAGTCATATGCGTTGGGGGTGGGTAAGAATATCGGTAGC TGGGGCGCCTTTTCCGCCGACGCCATCCATTCCCGATCCATGTCGACCGATGCCGGCAGGCAACAGGGCCAGTCGCTCCG GGTGCGCTATAGCAAAAATTTCCTGCAGACGGGAACAAATTTTACCATCGCCGGCTATCGCTATTCCACCTCCGGATTTC GCTCGCTGGAAGAAACGCTGGGTACCTACCGTAGCGATAATAACATTCCTTCTTATATCACCGATCGCCGGCGTAACCGG GCTGAGCTGTCGCTGAATCAGGATCTTGGCGATAACTTTGGGGCGTTGAATGCATCCTTGATCAGCGAGGATTACTGGCA GAACGAACGTAACAGCCTGTCGGCAAATCTGGGCTATTACAAAAATTTCAACGCGATTGGTATCAGTCTGAACTACTCCT GGAACCGGAATACCGGGCTCTATGGCCATGCCGAACAGGATAATGTGGTCTCCTTTGAGATCAATGTGCCGCTGGACAAA TTCCTCAACCGGACATACGCCTCATACGGGACAACCCATGCGAGTGGAAACGGAACCAGCCACAACCTCCGACTGAATGG TTCAGCCTTTGATGATGCCAGTCTTGACTGGGGGATAAACGCGACGCTTGCGGGGCGCAGCGAACAACAAGTCGTCGGGG GTAACGTCAGCTGGAAGGCAAGCCACGGAACGTTAAATGGTTCCTACAGCCGCAGCCGCGACAGCCAACAGCTGGGGTAT GGCATAGCCGGTGGGGTAGTGATTCATGAGAATGGTATCACGCTGTCCCAATCCCTGGGTGAAACTATTGGTTTAGTTAA GGCTCCTGGCGCAGAAGGCATTGGCGTCAATAACTACGCCGGTATCTCTACGGATAGTCGGGGTTACGCGGTTGTTCCGT ATCTGACCCCCTATCGCCGCAATGATATTAGCCTGAATAGTAAAACCCTGCGGGACGATACCGATATCACCTATATGACC AATAAAGTCGTCCCCACCCGCGGGGCTATCGTTAGAGCAGAGTATAAAACCGCCATTGGCCACCGTGTCCTGATGACGTT AACGCGGCCTGGGGGAAAACCGGTCCCCTTTGGCGCGATGGCGTCGCTGCTTAAAAGTGAAGAAAACGCGTCAATTGTCG GTGACGGCGGAGAAGTTCTGCTGAGCGGTTTACCGCAAAAAGGGACGCTGCAGGTGAAATGGGGGAATGGCCAGGGGCAG GAAACCACCTGTCGGGCGGATTATATAGCCCATAAATCTGCTGGCGCGTCCGGTCTCTGGAACGCGACGAGCGTCTGTCA ATAA
Upstream 100 bases:
>100_bases GAGTTTCACTCAAAATATTAATTTCTTCTGATTACATCTGATTAATGGGTATGGAATTTTGGGTCAGTTCAATTTCAATA TGAAATATGCATGCCTCTTG
Downstream 100 bases:
>100_bases CTCTGCGCCCTTGAGCAAAGTAAATAGCCGTCCTTACGAGGAAGAATAAAATGAAGTGGTTTGTTTTTATGTTCGCGGCA ATCGCGGCGACGCTGATGGT
Product: putative fimbrial usher
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 827; Mature: 827
Protein sequence:
>827_residues MMSALFNAPAISFADEFFNPALLEVREGEGAPTTDLSVFEVVNGQPAGNYRVDIYVNNEMVTADARDVMFSKNQDGKLEP CVTLAELSQWGVDTASFPELAVSDSECANLAGIPDAFADFQFNRQKLNLSIPHAAMNTRSLDEVPIERWDEGIPALLLNY NMNSYQSRYKGAETYESTYLNLRPGLNIGPWRLRNYTTFEHSNQSGSKWDNVYSYLQRNIVSLRSQLTLGDSSAPADVFD SVPFRGFQMASDDDMLPSSVRNYAPVIRGVARSNALVTIRQNGYVVYETKVAPGAFEISDLASTGGAGDLDVTIKEADGS EQHLRIPYASLPVLQREGKFRYSVTGGQFRAYDSHTEKNLFVQGTGIYGLPWGFTLYGGVQNAGNKYQSYALGVGKNIGS WGAFSADAIHSRSMSTDAGRQQGQSLRVRYSKNFLQTGTNFTIAGYRYSTSGFRSLEETLGTYRSDNNIPSYITDRRRNR AELSLNQDLGDNFGALNASLISEDYWQNERNSLSANLGYYKNFNAIGISLNYSWNRNTGLYGHAEQDNVVSFEINVPLDK FLNRTYASYGTTHASGNGTSHNLRLNGSAFDDASLDWGINATLAGRSEQQVVGGNVSWKASHGTLNGSYSRSRDSQQLGY GIAGGVVIHENGITLSQSLGETIGLVKAPGAEGIGVNNYAGISTDSRGYAVVPYLTPYRRNDISLNSKTLRDDTDITYMT NKVVPTRGAIVRAEYKTAIGHRVLMTLTRPGGKPVPFGAMASLLKSEENASIVGDGGEVLLSGLPQKGTLQVKWGNGQGQ ETTCRADYIAHKSAGASGLWNATSVCQ
Sequences:
>Translated_827_residues MMSALFNAPAISFADEFFNPALLEVREGEGAPTTDLSVFEVVNGQPAGNYRVDIYVNNEMVTADARDVMFSKNQDGKLEP CVTLAELSQWGVDTASFPELAVSDSECANLAGIPDAFADFQFNRQKLNLSIPHAAMNTRSLDEVPIERWDEGIPALLLNY NMNSYQSRYKGAETYESTYLNLRPGLNIGPWRLRNYTTFEHSNQSGSKWDNVYSYLQRNIVSLRSQLTLGDSSAPADVFD SVPFRGFQMASDDDMLPSSVRNYAPVIRGVARSNALVTIRQNGYVVYETKVAPGAFEISDLASTGGAGDLDVTIKEADGS EQHLRIPYASLPVLQREGKFRYSVTGGQFRAYDSHTEKNLFVQGTGIYGLPWGFTLYGGVQNAGNKYQSYALGVGKNIGS WGAFSADAIHSRSMSTDAGRQQGQSLRVRYSKNFLQTGTNFTIAGYRYSTSGFRSLEETLGTYRSDNNIPSYITDRRRNR AELSLNQDLGDNFGALNASLISEDYWQNERNSLSANLGYYKNFNAIGISLNYSWNRNTGLYGHAEQDNVVSFEINVPLDK FLNRTYASYGTTHASGNGTSHNLRLNGSAFDDASLDWGINATLAGRSEQQVVGGNVSWKASHGTLNGSYSRSRDSQQLGY GIAGGVVIHENGITLSQSLGETIGLVKAPGAEGIGVNNYAGISTDSRGYAVVPYLTPYRRNDISLNSKTLRDDTDITYMT NKVVPTRGAIVRAEYKTAIGHRVLMTLTRPGGKPVPFGAMASLLKSEENASIVGDGGEVLLSGLPQKGTLQVKWGNGQGQ ETTCRADYIAHKSAGASGLWNATSVCQ >Mature_827_residues MMSALFNAPAISFADEFFNPALLEVREGEGAPTTDLSVFEVVNGQPAGNYRVDIYVNNEMVTADARDVMFSKNQDGKLEP CVTLAELSQWGVDTASFPELAVSDSECANLAGIPDAFADFQFNRQKLNLSIPHAAMNTRSLDEVPIERWDEGIPALLLNY NMNSYQSRYKGAETYESTYLNLRPGLNIGPWRLRNYTTFEHSNQSGSKWDNVYSYLQRNIVSLRSQLTLGDSSAPADVFD SVPFRGFQMASDDDMLPSSVRNYAPVIRGVARSNALVTIRQNGYVVYETKVAPGAFEISDLASTGGAGDLDVTIKEADGS EQHLRIPYASLPVLQREGKFRYSVTGGQFRAYDSHTEKNLFVQGTGIYGLPWGFTLYGGVQNAGNKYQSYALGVGKNIGS WGAFSADAIHSRSMSTDAGRQQGQSLRVRYSKNFLQTGTNFTIAGYRYSTSGFRSLEETLGTYRSDNNIPSYITDRRRNR AELSLNQDLGDNFGALNASLISEDYWQNERNSLSANLGYYKNFNAIGISLNYSWNRNTGLYGHAEQDNVVSFEINVPLDK FLNRTYASYGTTHASGNGTSHNLRLNGSAFDDASLDWGINATLAGRSEQQVVGGNVSWKASHGTLNGSYSRSRDSQQLGY GIAGGVVIHENGITLSQSLGETIGLVKAPGAEGIGVNNYAGISTDSRGYAVVPYLTPYRRNDISLNSKTLRDDTDITYMT NKVVPTRGAIVRAEYKTAIGHRVLMTLTRPGGKPVPFGAMASLLKSEENASIVGDGGEVLLSGLPQKGTLQVKWGNGQGQ ETTCRADYIAHKSAGASGLWNATSVCQ
Specific function: Involved in the export and assembly of sfmA fimbrial subunits across the outer membrane [H]
COG id: COG3188
COG function: function code NU; P pilus assembly protein, porin PapC
Gene ontology:
Cell location: Cell outer membrane; Multi-pass membrane protein [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the fimbrial export usher family [H]
Homologues:
Organism=Escherichia coli, GI1786744, Length=854, Percent_Identity=39.8126463700234, Blast_Score=595, Evalue=1e-171, Organism=Escherichia coli, GI1790772, Length=848, Percent_Identity=40.9198113207547, Blast_Score=573, Evalue=1e-164, Organism=Escherichia coli, GI1787172, Length=862, Percent_Identity=38.2830626450116, Blast_Score=546, Evalue=1e-156, Organism=Escherichia coli, GI1789533, Length=816, Percent_Identity=38.6029411764706, Blast_Score=542, Evalue=1e-155, Organism=Escherichia coli, GI1786332, Length=839, Percent_Identity=33.969010727056, Blast_Score=397, Evalue=1e-111, Organism=Escherichia coli, GI1788427, Length=764, Percent_Identity=30.7591623036649, Blast_Score=332, Evalue=6e-92, Organism=Escherichia coli, GI87081778, Length=834, Percent_Identity=28.8968824940048, Blast_Score=252, Evalue=7e-68, Organism=Escherichia coli, GI1789610, Length=718, Percent_Identity=24.2339832869081, Blast_Score=126, Evalue=6e-30,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000015 - InterPro: IPR018030 [H]
Pfam domain/function: PF00577 Usher [H]
EC number: NA
Molecular weight: Translated: 90213; Mature: 90213
Theoretical pI: Translated: 5.99; Mature: 5.99
Prosite motif: PS01151 FIMBRIAL_USHER
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 1.9 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 1.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMSALFNAPAISFADEFFNPALLEVREGEGAPTTDLSVFEVVNGQPAGNYRVDIYVNNEM CCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEECCEE VTADARDVMFSKNQDGKLEPCVTLAELSQWGVDTASFPELAVSDSECANLAGIPDAFADF EECCHHHHEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCHHHCCCCCCHHHHCC QFNRQKLNLSIPHAAMNTRSLDEVPIERWDEGIPALLLNYNMNSYQSRYKGAETYESTYL EECCEEEEEECCCHHCCCCCCCCCCHHHHCCCCCEEEEECCCHHHHHHHCCCCCCCCEEE NLRPGLNIGPWRLRNYTTFEHSNQSGSKWDNVYSYLQRNIVSLRSQLTLGDSSAPADVFD EECCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHH SVPFRGFQMASDDDMLPSSVRNYAPVIRGVARSNALVTIRQNGYVVYETKVAPGAFEISD CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCCEEHHH LASTGGAGDLDVTIKEADGSEQHLRIPYASLPVLQREGKFRYSVTGGQFRAYDSHTEKNL HHCCCCCCCEEEEEECCCCCCCEEECCHHHCCCEECCCCEEEEEECCEEEEECCCCCCEE FVQGTGIYGLPWGFTLYGGVQNAGNKYQSYALGVGKNIGSWGAFSADAIHSRSMSTDAGR EEEECCEEECCCCEEEECCCCCCCCCHHEEEEECCCCCCCCCCCCHHHHHCCCCCCHHHH QQGQSLRVRYSKNFLQTGTNFTIAGYRYSTSGFRSLEETLGTYRSDNNIPSYITDRRRNR HCCCEEEEEEECHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCC AELSLNQDLGDNFGALNASLISEDYWQNERNSLSANLGYYKNFNAIGISLNYSWNRNTGL EEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCEEECCEEEEEEEEECCCCCCCE YGHAEQDNVVSFEINVPLDKFLNRTYASYGTTHASGNGTSHNLRLNGSAFDDASLDWGIN EEECCCCCEEEEEECCCHHHHHHHHHHHCCCEECCCCCCCEEEEECCCCCCCCCCCCCCE ATLAGRSEQQVVGGNVSWKASHGTLNGSYSRSRDSQQLGYGIAGGVVIHENGITLSQSLG EEECCCCCCEEECCCEEEEECCCCCCCCCCCCCCHHHHCCEEECCEEEECCCCEEHHHHH ETIGLVKAPGAEGIGVNNYAGISTDSRGYAVVPYLTPYRRNDISLNSKTLRDDTDITYMT HHCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEE NKVVPTRGAIVRAEYKTAIGHRVLMTLTRPGGKPVPFGAMASLLKSEENASIVGDGGEVL CEECCCCCCEEEEHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCHHHH LSGLPQKGTLQVKWGNGQGQETTCRADYIAHKSAGASGLWNATSVCQ EECCCCCCEEEEEECCCCCCCCEEHHHHHEECCCCCCCCCCCHHCCC >Mature Secondary Structure MMSALFNAPAISFADEFFNPALLEVREGEGAPTTDLSVFEVVNGQPAGNYRVDIYVNNEM CCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEECCEE VTADARDVMFSKNQDGKLEPCVTLAELSQWGVDTASFPELAVSDSECANLAGIPDAFADF EECCHHHHEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCHHHCCCCCCHHHHCC QFNRQKLNLSIPHAAMNTRSLDEVPIERWDEGIPALLLNYNMNSYQSRYKGAETYESTYL EECCEEEEEECCCHHCCCCCCCCCCHHHHCCCCCEEEEECCCHHHHHHHCCCCCCCCEEE NLRPGLNIGPWRLRNYTTFEHSNQSGSKWDNVYSYLQRNIVSLRSQLTLGDSSAPADVFD EECCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHH SVPFRGFQMASDDDMLPSSVRNYAPVIRGVARSNALVTIRQNGYVVYETKVAPGAFEISD CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCCEEHHH LASTGGAGDLDVTIKEADGSEQHLRIPYASLPVLQREGKFRYSVTGGQFRAYDSHTEKNL HHCCCCCCCEEEEEECCCCCCCEEECCHHHCCCEECCCCEEEEEECCEEEEECCCCCCEE FVQGTGIYGLPWGFTLYGGVQNAGNKYQSYALGVGKNIGSWGAFSADAIHSRSMSTDAGR EEEECCEEECCCCEEEECCCCCCCCCHHEEEEECCCCCCCCCCCCHHHHHCCCCCCHHHH QQGQSLRVRYSKNFLQTGTNFTIAGYRYSTSGFRSLEETLGTYRSDNNIPSYITDRRRNR HCCCEEEEEEECHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCC AELSLNQDLGDNFGALNASLISEDYWQNERNSLSANLGYYKNFNAIGISLNYSWNRNTGL EEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCEEECCEEEEEEEEECCCCCCCE YGHAEQDNVVSFEINVPLDKFLNRTYASYGTTHASGNGTSHNLRLNGSAFDDASLDWGIN EEECCCCCEEEEEECCCHHHHHHHHHHHCCCEECCCCCCCEEEEECCCCCCCCCCCCCCE ATLAGRSEQQVVGGNVSWKASHGTLNGSYSRSRDSQQLGYGIAGGVVIHENGITLSQSLG EEECCCCCCEEECCCEEEEECCCCCCCCCCCCCCHHHHCCEEECCEEEECCCCEEHHHHH ETIGLVKAPGAEGIGVNNYAGISTDSRGYAVVPYLTPYRRNDISLNSKTLRDDTDITYMT HHCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEE NKVVPTRGAIVRAEYKTAIGHRVLMTLTRPGGKPVPFGAMASLLKSEENASIVGDGGEVL CEECCCCCCEEEEHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCHHHH LSGLPQKGTLQVKWGNGQGQETTCRADYIAHKSAGASGLWNATSVCQ EECCCCCCEEEEEECCCCCCCCEEHHHHHEECCCCCCCCCCCHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 9278503 [H]