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The map label for this gene is yafE [H]

Identifier: 152968800

GI number: 152968800

Start: 258933

End: 259703

Strand: Direct

Name: yafE [H]

Synonym: KPN_00225

Alternate gene names: 152968800

Gene position: 258933-259703 (Clockwise)

Preceding gene: 152968799

Following gene: 152968803

Centisome position: 4.87

GC content: 58.63

Gene sequence:

>771_bases
ATGACAACACGCTCTCACCATGACAACGTCGAAAAGCAGTTCGGCTCCCAGGCCAGCGCCTATCTGACCAGCGCCGTACA
CGCCTCTGGTCGCGATCTGCAGCGGCTGGCGGAACGGCTGGCTGATTTTCCTCAGGCGAAAGTGCTGGATATGGGCTGCG
GGGCAGGGCACGCCAGTTTTACCGCGGCGGGACAGGTTGCCGAGGTGACCGCCTATGATTTATCGAGCCAGATGCTGGAG
GTCGTTGCCGCCGCGGCAAAGGAGAAAGGTTTTAGCAATATCGTGACCCAGCAAGGGTACGCCGAAACGCTGCCGTTCGC
TGATGTCAGCTTTGATGTGGTGATTAGCCGCTACTCTGCTCACCACTGGCATGACGTTGGCCAGGCGCTGCGTGAGGTGA
AACGTGTTCTCAAGCCGGGCGGGGTGATTATCATGATGGACGTGATGTCGCCGGGTCATCCGGTACGTGATGTCTGGCTG
CAGACGGTGGAAGCCCTGCGCGACACCTCGCATGTGCGTAATTATTCCAGCGGCGAGTGGTTGACGCTGGCGACTGAAGC
GGGGCTGGTGGTTAACCAGTTGTTGACCGATCGCCTGCCGCTGGAGTTCAGTTCATGGGTGGCGCGGATGCGTACTCCTG
AGCCGCTGGTGGAGGCGATTCGCTTATATCAGCAGAGCGCTTCGGCGGAGGTCAAAGCGTACTTCGAGTTACAGGAGGAC
GGATCGTTTACCAGCGATACCATTCTGTTCGAAGCGCATAAAGCGGTTTAA

Upstream 100 bases:

>100_bases
CCTGATTAATTTAAGGTTGGTCAGGCCTGCCATGGGGCAGGCCTGCGCATGCATGGTGCTGCCTTTTATTACTCAAAAAA
AACCGTAAGGACAAGACAAC

Downstream 100 bases:

>100_bases
TTTAGGCAACAAAAAAGGCACCGGGGAAGTCGGTGCCTTTTTGTCGAACCAGAGGTATCAGGACTCTGGTCGATCGTTAT
TTTTCACAAACAACGTTAGC

Product: putative biotin synthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTTRSHHDNVEKQFGSQASAYLTSAVHASGRDLQRLAERLADFPQAKVLDMGCGAGHASFTAAGQVAEVTAYDLSSQMLE
VVAAAAKEKGFSNIVTQQGYAETLPFADVSFDVVISRYSAHHWHDVGQALREVKRVLKPGGVIIMMDVMSPGHPVRDVWL
QTVEALRDTSHVRNYSSGEWLTLATEAGLVVNQLLTDRLPLEFSSWVARMRTPEPLVEAIRLYQQSASAEVKAYFELQED
GSFTSDTILFEAHKAV

Sequences:

>Translated_256_residues
MTTRSHHDNVEKQFGSQASAYLTSAVHASGRDLQRLAERLADFPQAKVLDMGCGAGHASFTAAGQVAEVTAYDLSSQMLE
VVAAAAKEKGFSNIVTQQGYAETLPFADVSFDVVISRYSAHHWHDVGQALREVKRVLKPGGVIIMMDVMSPGHPVRDVWL
QTVEALRDTSHVRNYSSGEWLTLATEAGLVVNQLLTDRLPLEFSSWVARMRTPEPLVEAIRLYQQSASAEVKAYFELQED
GSFTSDTILFEAHKAV
>Mature_255_residues
TTRSHHDNVEKQFGSQASAYLTSAVHASGRDLQRLAERLADFPQAKVLDMGCGAGHASFTAAGQVAEVTAYDLSSQMLEV
VAAAAKEKGFSNIVTQQGYAETLPFADVSFDVVISRYSAHHWHDVGQALREVKRVLKPGGVIIMMDVMSPGHPVRDVWLQ
TVEALRDTSHVRNYSSGEWLTLATEAGLVVNQLLTDRLPLEFSSWVARMRTPEPLVEAIRLYQQSASAEVKAYFELQEDG
SFTSDTILFEAHKAV

Specific function: Unknown

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786404, Length=204, Percent_Identity=72.0588235294118, Blast_Score=318, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: NA

Molecular weight: Translated: 28166; Mature: 28035

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRSHHDNVEKQFGSQASAYLTSAVHASGRDLQRLAERLADFPQAKVLDMGCGAGHASF
CCCCCCCCHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCH
TAAGQVAEVTAYDLSSQMLEVVAAAAKEKGFSNIVTQQGYAETLPFADVSFDVVISRYSA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHCCCCCCHHHHHHHHHHH
HHWHDVGQALREVKRVLKPGGVIIMMDVMSPGHPVRDVWLQTVEALRDTSHVRNYSSGEW
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCE
LTLATEAGLVVNQLLTDRLPLEFSSWVARMRTPEPLVEAIRLYQQSASAEVKAYFELQED
EEEECHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCC
GSFTSDTILFEAHKAV
CCCCCCEEEEEECCCC
>Mature Secondary Structure 
TTRSHHDNVEKQFGSQASAYLTSAVHASGRDLQRLAERLADFPQAKVLDMGCGAGHASF
CCCCCCCHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCH
TAAGQVAEVTAYDLSSQMLEVVAAAAKEKGFSNIVTQQGYAETLPFADVSFDVVISRYSA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHCCCCCCHHHHHHHHHHH
HHWHDVGQALREVKRVLKPGGVIIMMDVMSPGHPVRDVWLQTVEALRDTSHVRNYSSGEW
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCE
LTLATEAGLVVNQLLTDRLPLEFSSWVARMRTPEPLVEAIRLYQQSASAEVKAYFELQED
EEEECHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCC
GSFTSDTILFEAHKAV
CCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1663890; 9278503 [H]