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The map label for this gene is yaeL [H]

Identifier: 152968770

GI number: 152968770

Start: 222297

End: 223664

Strand: Direct

Name: yaeL [H]

Synonym: KPN_00189

Alternate gene names: 152968770

Gene position: 222297-223664 (Clockwise)

Preceding gene: 152968769

Following gene: 152968771

Centisome position: 4.18

GC content: 54.82

Gene sequence:

>1368_bases
TTGACGGAAGGTTATATGCTGAGCGTGCTCTGGAATCTGGCGGCTTTTATCATTGCGCTTGGCGTACTGATTACGGTGCA
TGAGTTTGGCCATTTTTGGGTGGCGCGCCGCTGCGGTATCCGGGTTGAGCGTTTTTCTATCGGCTTCGGCAAAGCGCTGT
GGCGGCGGATGGATAAGCAGGGCACAGAGTTCGTCATCGCCCTGATCCCACTGGGCGGCTACGTCAAGATGCTGGATGAG
CGTGTTGAAGCGGTGGCGCCGGAGATGCGTCACTACGCCTTCAACAATAAAACTGTCGGTCAGCGTGCGGCGGTTATTGC
CGCCGGCCCCATCGCTAACTTCATTTTCGCTATCTTTGCTTACTGGCTGGTCTTTATCATCGGCGTTCCCGGCGTGCGGC
CGGTGGTGGGTGAAATAACGCCCAATTCGGTTGCCGCGCAAGCACAAATTGCAAAAGGAACGGAACTTAAAGCGATAGAT
GGTATCGAAACGCCTGATTGGGATGCGGTGCGTCTGCAGCTGGTGGCCAAAATCGGCAACCCGCAAACAATAGTTACCGT
CGCCCCTTTCGGGACCAATCAGCGCCAGGATAAAATCGTCGATCTGCGGCACTGGTCGTTTGAGCCAGACAAGCAAGATC
CCGTGACCTCTCTGGGGATCCAGCCGCGCAGCGCGCAGATTGATACGGTGCTGGCTGAGGTTCAGGCGGGATCGGCAGCC
CAGAAAGCGGGTTTGCAAGCGGGCGACCGGATCGTTAAAGTCGATGGTCAACCGTTAACGCAGTGGATGACGTTTGTTAA
TCTGGTGCGCGATAATCCGGGCAAAGCGCTGGCGCTGGAGATTGAACGGCAGGGGAGTGCGCTGCCGTTAACCCTGACGC
CGGATGCGAAAACGGTGAAAGGTAAGGCGGAAGGGTTCGCAGGGGTGGTGCCAAAGGTCATCCCGCTGCCTGAAGAATAC
AAGACAGTACGTCAGTACGGACCGTTTGCCGCCATCGCTGAAGCCACGGATAAAACCTGGCAACTGATGTCGCTGACGGT
CAGGATGCTGGGTAAATTGATAACCGGTGATGTCAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCTCAGGGGGCTG
GAATGTCAGCGGAGTTTGGGTTGATTTATTATCTGATGTTCCTGGCGCTGATTAGCGTGAACCTCGGAATCATCAACCTG
TTCCCTCTACCCGTACTGGATGGGGGACATCTGCTGTTTTTAGCGATTGAAAAGCTGAAGGGCGGGCCGGTATCCGAGCG
AGTTCAAGACTTTAGTTATCGCATTGGCTCAATACTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTC
GGTTATAA

Upstream 100 bases:

>100_bases
ACTTGATTCCCGGACATGGTGGTATTCTCGATCGTATCGACAGCCTGACGGCGGCGGTACCGGTCTTCGCCTGTCTGCTA
CTGTTGGTCTTCAGGACGAT

Downstream 100 bases:

>100_bases
GAGAGTTTGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCGA
CTGTATACGGTGCTGAAGGG

Product: zinc metallopeptidase RseP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTEGYMLSVLWNLAAFIIALGVLITVHEFGHFWVARRCGIRVERFSIGFGKALWRRMDKQGTEFVIALIPLGGYVKMLDE
RVEAVAPEMRHYAFNNKTVGQRAAVIAAGPIANFIFAIFAYWLVFIIGVPGVRPVVGEITPNSVAAQAQIAKGTELKAID
GIETPDWDAVRLQLVAKIGNPQTIVTVAPFGTNQRQDKIVDLRHWSFEPDKQDPVTSLGIQPRSAQIDTVLAEVQAGSAA
QKAGLQAGDRIVKVDGQPLTQWMTFVNLVRDNPGKALALEIERQGSALPLTLTPDAKTVKGKAEGFAGVVPKVIPLPEEY
KTVRQYGPFAAIAEATDKTWQLMSLTVRMLGKLITGDVKLNNLSGPISIAQGAGMSAEFGLIYYLMFLALISVNLGIINL
FPLPVLDGGHLLFLAIEKLKGGPVSERVQDFSYRIGSILLVLLMGLALFNDFSRL

Sequences:

>Translated_455_residues
MTEGYMLSVLWNLAAFIIALGVLITVHEFGHFWVARRCGIRVERFSIGFGKALWRRMDKQGTEFVIALIPLGGYVKMLDE
RVEAVAPEMRHYAFNNKTVGQRAAVIAAGPIANFIFAIFAYWLVFIIGVPGVRPVVGEITPNSVAAQAQIAKGTELKAID
GIETPDWDAVRLQLVAKIGNPQTIVTVAPFGTNQRQDKIVDLRHWSFEPDKQDPVTSLGIQPRSAQIDTVLAEVQAGSAA
QKAGLQAGDRIVKVDGQPLTQWMTFVNLVRDNPGKALALEIERQGSALPLTLTPDAKTVKGKAEGFAGVVPKVIPLPEEY
KTVRQYGPFAAIAEATDKTWQLMSLTVRMLGKLITGDVKLNNLSGPISIAQGAGMSAEFGLIYYLMFLALISVNLGIINL
FPLPVLDGGHLLFLAIEKLKGGPVSERVQDFSYRIGSILLVLLMGLALFNDFSRL
>Mature_454_residues
TEGYMLSVLWNLAAFIIALGVLITVHEFGHFWVARRCGIRVERFSIGFGKALWRRMDKQGTEFVIALIPLGGYVKMLDER
VEAVAPEMRHYAFNNKTVGQRAAVIAAGPIANFIFAIFAYWLVFIIGVPGVRPVVGEITPNSVAAQAQIAKGTELKAIDG
IETPDWDAVRLQLVAKIGNPQTIVTVAPFGTNQRQDKIVDLRHWSFEPDKQDPVTSLGIQPRSAQIDTVLAEVQAGSAAQ
KAGLQAGDRIVKVDGQPLTQWMTFVNLVRDNPGKALALEIERQGSALPLTLTPDAKTVKGKAEGFAGVVPKVIPLPEEYK
TVRQYGPFAAIAEATDKTWQLMSLTVRMLGKLITGDVKLNNLSGPISIAQGAGMSAEFGLIYYLMFLALISVNLGIINLF
PLPVLDGGHLLFLAIEKLKGGPVSERVQDFSYRIGSILLVLLMGLALFNDFSRL

Specific function: Cleaves the peptide bond between 'Ala-108' and 'Cys-109' in the transmembrane region of rseA. Acts on degS-cleaved rseA to release the cytoplasmic domain of rseA. This provides the cell with sigma E (rpoE) activity through the proteolysis of rseA. In vitr

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=450, Percent_Identity=83.1111111111111, Blast_Score=749, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 49605; Mature: 49474

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS50106 PDZ ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGYMLSVLWNLAAFIIALGVLITVHEFGHFWVARRCGIRVERFSIGFGKALWRRMDKQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCC
GTEFVIALIPLGGYVKMLDERVEAVAPEMRHYAFNNKTVGQRAAVIAAGPIANFIFAIFA
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHH
YWLVFIIGVPGVRPVVGEITPNSVAAQAQIAKGTELKAIDGIETPDWDAVRLQLVAKIGN
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCC
PQTIVTVAPFGTNQRQDKIVDLRHWSFEPDKQDPVTSLGIQPRSAQIDTVLAEVQAGSAA
CCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCHH
QKAGLQAGDRIVKVDGQPLTQWMTFVNLVRDNPGKALALEIERQGSALPLTLTPDAKTVK
HHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCC
GKAEGFAGVVPKVIPLPEEYKTVRQYGPFAAIAEATDKTWQLMSLTVRMLGKLITGDVKL
CCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEE
NNLSGPISIAQGAGMSAEFGLIYYLMFLALISVNLGIINLFPLPVLDGGHLLFLAIEKLK
ECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEEEEHHC
GGPVSERVQDFSYRIGSILLVLLMGLALFNDFSRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEGYMLSVLWNLAAFIIALGVLITVHEFGHFWVARRCGIRVERFSIGFGKALWRRMDKQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCC
GTEFVIALIPLGGYVKMLDERVEAVAPEMRHYAFNNKTVGQRAAVIAAGPIANFIFAIFA
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHH
YWLVFIIGVPGVRPVVGEITPNSVAAQAQIAKGTELKAIDGIETPDWDAVRLQLVAKIGN
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCC
PQTIVTVAPFGTNQRQDKIVDLRHWSFEPDKQDPVTSLGIQPRSAQIDTVLAEVQAGSAA
CCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCHH
QKAGLQAGDRIVKVDGQPLTQWMTFVNLVRDNPGKALALEIERQGSALPLTLTPDAKTVK
HHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCC
GKAEGFAGVVPKVIPLPEEYKTVRQYGPFAAIAEATDKTWQLMSLTVRMLGKLITGDVKL
CCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEE
NNLSGPISIAQGAGMSAEFGLIYYLMFLALISVNLGIINLFPLPVLDGGHLLFLAIEKLK
ECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEEEEHHC
GGPVSERVQDFSYRIGSILLVLLMGLALFNDFSRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]