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The map label for this gene is yadI [H]

Identifier: 152968718

GI number: 152968718

Start: 163163

End: 163603

Strand: Direct

Name: yadI [H]

Synonym: KPN_00137

Alternate gene names: 152968718

Gene position: 163163-163603 (Clockwise)

Preceding gene: 152968717

Following gene: 152968719

Centisome position: 3.07

GC content: 58.96

Gene sequence:

>441_bases
ATGCTCGGATGGGTCATTACCTGTCACGATGAGCTGGCGCAGGAGATGCTGGATCGTCTTGAACAAAAGTTTGGTCCGCT
GGCCCAGTGCCGGGCGGTCAATTACTGGCGCAATCTCAGCAGCAATATGTTGAGCCGGATGATGTGCGATGCGCTGCATG
CCACCGACTCCGGGGACGGGGTCATTTTTCTCACTGACAAAACCGGTGCGGCGCCTTATCGGGCCGCGGCGCTGATGAGT
CACAAACATACCCACTGCGAAGTGATCTCCGGCGTCAGCTATCCACTCCTTGAGCGAATGTATCTCCTGCGAGGCACGTT
AAGCAGCGTGGCGTTTCGCCAGGCGATCGTCAGCGCGGGCGGGCCGATGGTGAGCAGCCTGTGGCATCAGCAGCAGAAGA
ACCCCCCGTTCCGGTTGCGCCATGATGCCTGGGGAAATTAA

Upstream 100 bases:

>100_bases
CTGAAGTAAATATGCGAAGCGCTAACCGCTTATCTTTGACTCCCATCACCGATACGTCATCTTCACCTTGCTACATTATT
CGCTGGCTAAGGAGGGAAGC

Downstream 100 bases:

>100_bases
GCAGCGGGACAGATACCGCTTTTGTTACAATAACCGCCGTTGATGGTATAACGGTTTGATGCGTATGCTTGCCCGATTTT
TGACCCTGCTTCTTTTCCTG

Product: putative PTS enzyme II B component

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MLGWVITCHDELAQEMLDRLEQKFGPLAQCRAVNYWRNLSSNMLSRMMCDALHATDSGDGVIFLTDKTGAAPYRAAALMS
HKHTHCEVISGVSYPLLERMYLLRGTLSSVAFRQAIVSAGGPMVSSLWHQQQKNPPFRLRHDAWGN

Sequences:

>Translated_146_residues
MLGWVITCHDELAQEMLDRLEQKFGPLAQCRAVNYWRNLSSNMLSRMMCDALHATDSGDGVIFLTDKTGAAPYRAAALMS
HKHTHCEVISGVSYPLLERMYLLRGTLSSVAFRQAIVSAGGPMVSSLWHQQQKNPPFRLRHDAWGN
>Mature_146_residues
MLGWVITCHDELAQEMLDRLEQKFGPLAQCRAVNYWRNLSSNMLSRMMCDALHATDSGDGVIFLTDKTGAAPYRAAALMS
HKHTHCEVISGVSYPLLERMYLLRGTLSSVAFRQAIVSAGGPMVSSLWHQQQKNPPFRLRHDAWGN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1786321, Length=144, Percent_Identity=69.4444444444444, Blast_Score=218, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701 [H]

Pfam domain/function: PF03610 EIIA-man [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16389; Mature: 16389

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGWVITCHDELAQEMLDRLEQKFGPLAQCRAVNYWRNLSSNMLSRMMCDALHATDSGDG
CCCEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VIFLTDKTGAAPYRAAALMSHKHTHCEVISGVSYPLLERMYLLRGTLSSVAFRQAIVSAG
EEEEECCCCCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPMVSSLWHQQQKNPPFRLRHDAWGN
CHHHHHHHHHHHCCCCCEECCCCCCC
>Mature Secondary Structure
MLGWVITCHDELAQEMLDRLEQKFGPLAQCRAVNYWRNLSSNMLSRMMCDALHATDSGDG
CCCEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VIFLTDKTGAAPYRAAALMSHKHTHCEVISGVSYPLLERMYLLRGTLSSVAFRQAIVSAG
EEEEECCCCCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPMVSSLWHQQQKNPPFRLRHDAWGN
CHHHHHHHHHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503 [H]