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The map label for this gene is ampD [H]

Identifier: 152968693

GI number: 152968693

Start: 130556

End: 131119

Strand: Direct

Name: ampD [H]

Synonym: KPN_00112

Alternate gene names: 152968693

Gene position: 130556-131119 (Clockwise)

Preceding gene: 152968688

Following gene: 152968694

Centisome position: 2.46

GC content: 58.69

Gene sequence:

>564_bases
ATGCAGTTGAACAAGGGGTGGTTGGTCGGCGCGCGTCGGGTTCCCTCGCCGCACCATGACTGCCGTCCGGATGAAGAGAC
CCCTTCCTTACTGGTGGTGCATAACATTAGCCTGCCACCGGGGGAATTTGGTGGTCCGTGGATTGATGCGCTGTTCACTG
GTACCCTGGATCCGCACGCGCATCCTTTCTTTGCCGAGATTGCTCATCTGCGCGTTTCGGCGCATTGTCTGATTCGCCGT
GATGGCGAAATCGTGCAGTATGTGCCTTTCGATAAGCGGGCGTGGCATGCGGGCGTCTCCTGTTATCAGGGACGCGAACG
CTGCAATGACTTTTCCATCGGCATAGAGCTGGAAGGCACCGATACTCTGGCGTATACCGACGCGCAGTACCGGCAGCTGG
CGGCGGTGACGGATCTGCTGATCGCGCTTTATCCGGCTATCGCCGAAAACATTGCGGGCCACAGCGATATCGCGCCCGTG
CGGAAAACCGATCCAGGACCGGCCTTTGACTGGATAAAATACCGTGCGCTGCTCAGCGCCCCGTCAGAAAAGGAGACCTC
ATGA

Upstream 100 bases:

>100_bases
GGAAACATGCTAACCTGAACGACGTTTCAGTACCATACACACTTCATCCTTTGGGCCGGCGTGTGGCCCGGAAGCATGAG
TTCGCGAAAGGAGATCCCGC

Downstream 100 bases:

>100_bases
CGCTGTTTACGACGCTGTTGGTGCTTATCGCCGAACGGCTGTTTAAGCTGGGCGAACACTGGCAGCTTGATCATCGGCTG
GAGGTGGCGTTTCGCCGGAT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MQLNKGWLVGARRVPSPHHDCRPDEETPSLLVVHNISLPPGEFGGPWIDALFTGTLDPHAHPFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSCYQGRERCNDFSIGIELEGTDTLAYTDAQYRQLAAVTDLLIALYPAIAENIAGHSDIAPV
RKTDPGPAFDWIKYRALLSAPSEKETS

Sequences:

>Translated_187_residues
MQLNKGWLVGARRVPSPHHDCRPDEETPSLLVVHNISLPPGEFGGPWIDALFTGTLDPHAHPFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSCYQGRERCNDFSIGIELEGTDTLAYTDAQYRQLAAVTDLLIALYPAIAENIAGHSDIAPV
RKTDPGPAFDWIKYRALLSAPSEKETS
>Mature_187_residues
MQLNKGWLVGARRVPSPHHDCRPDEETPSLLVVHNISLPPGEFGGPWIDALFTGTLDPHAHPFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSCYQGRERCNDFSIGIELEGTDTLAYTDAQYRQLAAVTDLLIALYPAIAENIAGHSDIAPV
RKTDPGPAFDWIKYRALLSAPSEKETS

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=187, Percent_Identity=83.9572192513369, Blast_Score=327, Evalue=4e-91,
Organism=Escherichia coli, GI1787092, Length=124, Percent_Identity=41.1290322580645, Blast_Score=79, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20753; Mature: 20753

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLNKGWLVGARRVPSPHHDCRPDEETPSLLVVHNISLPPGEFGGPWIDALFTGTLDPHA
CCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCCCCCC
HPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSCYQGRERCNDFSIGIELEGT
CHHHHHHHHHHHHEEEEEECCCCEEEECCCCCHHHHHCCHHHHHHHHCCCEEEEEEECCC
DTLAYTDAQYRQLAAVTDLLIALYPAIAENIAGHSDIAPVRKTDPGPAFDWIKYRALLSA
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
PSEKETS
CCCCCCC
>Mature Secondary Structure
MQLNKGWLVGARRVPSPHHDCRPDEETPSLLVVHNISLPPGEFGGPWIDALFTGTLDPHA
CCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCCCCCC
HPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSCYQGRERCNDFSIGIELEGT
CHHHHHHHHHHHHEEEEEECCCCEEEECCCCCHHHHHCCHHHHHHHHCCCEEEEEEECCC
DTLAYTDAQYRQLAAVTDLLIALYPAIAENIAGHSDIAPVRKTDPGPAFDWIKYRALLSA
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
PSEKETS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503; 7959070 [H]