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The map label for this gene is ampE [H]

Identifier: 152968694

GI number: 152968694

Start: 131116

End: 131970

Strand: Direct

Name: ampE [H]

Synonym: KPN_00113

Alternate gene names: 152968694

Gene position: 131116-131970 (Clockwise)

Preceding gene: 152968693

Following gene: 152968698

Centisome position: 2.47

GC content: 59.42

Gene sequence:

>855_bases
ATGACGCTGTTTACGACGCTGTTGGTGCTTATCGCCGAACGGCTGTTTAAGCTGGGCGAACACTGGCAGCTTGATCATCG
GCTGGAGGTGGCGTTTCGCCGGATAAAACATTTCTCGCTACCGGGCACGCTGCTGATGACGCTGGTGGCCGTCGCGGTCG
TGTATATCATTCAGCGTCTGCTGCAGGGGCAGCTGTTTAACATACCGCTGCTGGTGTTCTGGATTTTACTGGGCCTGCTA
TGCATTGGCGCCGGCAAAGTTCGGCTGCACTACCATGCCTATCTGAAAGCGGCGTCGCGCAATGACAGTCGCGCCTGCGA
TGCCATGGCCAGCGAACTGACGCTGATTCACGGCGTGCCGCCGGGCTGTGATGAGCGTGAGTACCTTAGCGAACTGCAAA
ATGCGCTGCTGTGGATTAACTATCGATACTATCTGGCGCCGCTTTTCTGGTTTGTCGTGGGGGGCGCCTGGGGCCCGCTG
ACGCTTATCGCCTACAGCGTACTGCGCGCCTGGCAGACATGGCTGGCGCGCTACCAGACGCCGCACCACCGGCTGCAGTC
CGGGATCGATGGTATCCTGCATATTGTGGACTGGCTGCCGGTGCGTCTGGTGGGGGTGGTCTATGCCCTGGTGGGCCATG
GTGAAAAAGCGCTCCCGGCGTGGTTTGCTTCGCTGGGCGACCGCCATTCGTCGCAGTATCAGGTGTTGACCCGCCTGGCG
CAGTATTCCCTGGCCCGTGAGCCGCACGTGGATAAAGTAGCGACGCCGAAGGCTGCGGTATCGATGGCTAAGAAGACTTC
GCTGGTGGTAGTGGTGATTATGGCGCTGCTGACGATTTACGGTACCCTGGTTTAA

Upstream 100 bases:

>100_bases
ACAGCGATATCGCGCCCGTGCGGAAAACCGATCCAGGACCGGCCTTTGACTGGATAAAATACCGTGCGCTGCTCAGCGCC
CCGTCAGAAAAGGAGACCTC

Downstream 100 bases:

>100_bases
CGGCCTGAGCCCACGCGCTGCCTGCCACGATGTACGTACCTGCGGGGCTTTTGTTGCCTGATGGCGCTGCGCTTATCCGG
CCTGGCGAGTCACCCGCAGG

Product: regulatory protein AmpE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTLFTTLLVLIAERLFKLGEHWQLDHRLEVAFRRIKHFSLPGTLLMTLVAVAVVYIIQRLLQGQLFNIPLLVFWILLGLL
CIGAGKVRLHYHAYLKAASRNDSRACDAMASELTLIHGVPPGCDEREYLSELQNALLWINYRYYLAPLFWFVVGGAWGPL
TLIAYSVLRAWQTWLARYQTPHHRLQSGIDGILHIVDWLPVRLVGVVYALVGHGEKALPAWFASLGDRHSSQYQVLTRLA
QYSLAREPHVDKVATPKAAVSMAKKTSLVVVVIMALLTIYGTLV

Sequences:

>Translated_284_residues
MTLFTTLLVLIAERLFKLGEHWQLDHRLEVAFRRIKHFSLPGTLLMTLVAVAVVYIIQRLLQGQLFNIPLLVFWILLGLL
CIGAGKVRLHYHAYLKAASRNDSRACDAMASELTLIHGVPPGCDEREYLSELQNALLWINYRYYLAPLFWFVVGGAWGPL
TLIAYSVLRAWQTWLARYQTPHHRLQSGIDGILHIVDWLPVRLVGVVYALVGHGEKALPAWFASLGDRHSSQYQVLTRLA
QYSLAREPHVDKVATPKAAVSMAKKTSLVVVVIMALLTIYGTLV
>Mature_283_residues
TLFTTLLVLIAERLFKLGEHWQLDHRLEVAFRRIKHFSLPGTLLMTLVAVAVVYIIQRLLQGQLFNIPLLVFWILLGLLC
IGAGKVRLHYHAYLKAASRNDSRACDAMASELTLIHGVPPGCDEREYLSELQNALLWINYRYYLAPLFWFVVGGAWGPLT
LIAYSVLRAWQTWLARYQTPHHRLQSGIDGILHIVDWLPVRLVGVVYALVGHGEKALPAWFASLGDRHSSQYQVLTRLAQ
YSLAREPHVDKVATPKAAVSMAKKTSLVVVVIMALLTIYGTLV

Specific function: Putative signaling protein in beta-lactamase regulation. AmpE seems not to act as a direct sensor for beta-lactams [H]

COG id: COG3725

COG function: function code V; Membrane protein required for beta-lactamase induction

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786301, Length=284, Percent_Identity=79.2253521126761, Blast_Score=457, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: NA

Molecular weight: Translated: 32060; Mature: 31929

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLFTTLLVLIAERLFKLGEHWQLDHRLEVAFRRIKHFSLPGTLLMTLVAVAVVYIIQRL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LQGQLFNIPLLVFWILLGLLCIGAGKVRLHYHAYLKAASRNDSRACDAMASELTLIHGVP
HCCCCCCCHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
PGCDEREYLSELQNALLWINYRYYLAPLFWFVVGGAWGPLTLIAYSVLRAWQTWLARYQT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
PHHRLQSGIDGILHIVDWLPVRLVGVVYALVGHGEKALPAWFASLGDRHSSQYQVLTRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHH
QYSLAREPHVDKVATPKAAVSMAKKTSLVVVVIMALLTIYGTLV
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503 [H]