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The map label for this gene is yabF

Identifier: 152968624

GI number: 152968624

Start: 47477

End: 47953

Strand: Direct

Name: yabF

Synonym: KPN_00043

Alternate gene names: 152968624

Gene position: 47477-47953 (Clockwise)

Preceding gene: 152968623

Following gene: 152968625

Centisome position: 0.89

GC content: 59.33

Gene sequence:

>477_bases
ATGCTTGAACAGGCAGGGACGCTTGAAGGCGTAGAGATACGCTCCCTCTATCAACTCTATCCCGACTTTAATATCGATGT
CGCCGCCGAGCAGGCGGCGCTGGCCCGTGCCGATCTGGTTATCTGGCAGCACCCTATGCAGTGGTATAGCGTACCGCCGC
TGCTCAAGCTGTGGATGGACAAGGTGCTGTCGCACGGCTGGGCCTATGGTCACAACGGTATTGCCCTGCGCGGTAAATCG
CTGATGTGGGCGGTGACCACCGGCGGCGGTGAAAGCCACTTCGACATCGGCTCATTCCCCGGTTTTCCGGTGCTGGCGCA
GCCGTTGCAGGCAACCGCGCTTTACTGCGGAATGAAATGGCTGCCGCCGTTCGCTATGCACTGCACCTTTATCTGCGATG
ATGAAACGCTGCAGGCGCAGGCCCGCCGCTATCGTCAACGCTTAATCGACTGGCAGGAGGCACACCAGAATGGATAG

Upstream 100 bases:

>100_bases
TACACTCTTGCTGATTACGACAAGAGCAAACGCAGGCCAATCAATGATTCTGATAATTTATGCGCATCCTTATCCGCAGC
ATTCTCATGCGAATAAGCGA

Downstream 100 bases:

>100_bases
CCATACGCTGATACAGGCGTTGATTTACCTCGGCGCCGCGGCGCTGATTGTGCCCATCGCCGTCCGTCTGGGGCTGGGGT
CAGTGCTGGGCTATCTCATC

Product: glutathione-regulated potassium-efflux system ancillary protein KefF

Products: NAD(P); reduced acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MLEQAGTLEGVEIRSLYQLYPDFNIDVAAEQAALARADLVIWQHPMQWYSVPPLLKLWMDKVLSHGWAYGHNGIALRGKS
LMWAVTTGGGESHFDIGSFPGFPVLAQPLQATALYCGMKWLPPFAMHCTFICDDETLQAQARRYRQRLIDWQEAHQNG

Sequences:

>Translated_158_residues
MLEQAGTLEGVEIRSLYQLYPDFNIDVAAEQAALARADLVIWQHPMQWYSVPPLLKLWMDKVLSHGWAYGHNGIALRGKS
LMWAVTTGGGESHFDIGSFPGFPVLAQPLQATALYCGMKWLPPFAMHCTFICDDETLQAQARRYRQRLIDWQEAHQNG
>Mature_158_residues
MLEQAGTLEGVEIRSLYQLYPDFNIDVAAEQAALARADLVIWQHPMQWYSVPPLLKLWMDKVLSHGWAYGHNGIALRGKS
LMWAVTTGGGESHFDIGSFPGFPVLAQPLQATALYCGMKWLPPFAMHCTFICDDETLQAQARRYRQRLIDWQEAHQNG

Specific function: Required for full activity of kefC

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily

Homologues:

Organism=Homo sapiens, GI156564357, Length=151, Percent_Identity=35.7615894039735, Blast_Score=74, Evalue=6e-14,
Organism=Homo sapiens, GI4505415, Length=104, Percent_Identity=36.5384615384615, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1786231, Length=156, Percent_Identity=81.4102564102564, Blast_Score=282, Evalue=8e-78,
Organism=Escherichia coli, GI1789750, Length=146, Percent_Identity=36.986301369863, Blast_Score=97, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KEFF_KLEAE (Q9X755)

Other databases:

- EMBL:   AJ242913
- ProteinModelPortal:   Q9X755
- SMR:   Q9X755
- HAMAP:   MF_01414
- InterPro:   IPR003680

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 1.6.99.- [C]

Molecular weight: Translated: 17890; Mature: 17890

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEQAGTLEGVEIRSLYQLYPDFNIDVAAEQAALARADLVIWQHPMQWYSVPPLLKLWMD
CCCCCCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHEEECCHHHCCCCHHHHHHHH
KVLSHGWAYGHNGIALRGKSLMWAVTTGGGESHFDIGSFPGFPVLAQPLQATALYCGMKW
HHHHCCCCCCCCCEEEECCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
LPPFAMHCTFICDDETLQAQARRYRQRLIDWQEAHQNG
CCCHHHEEEEEECCHHHHHHHHHHHHHHCCHHHHCCCC
>Mature Secondary Structure
MLEQAGTLEGVEIRSLYQLYPDFNIDVAAEQAALARADLVIWQHPMQWYSVPPLLKLWMD
CCCCCCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHEEECCHHHCCCCHHHHHHHH
KVLSHGWAYGHNGIALRGKSLMWAVTTGGGESHFDIGSFPGFPVLAQPLQATALYCGMKW
HHHHCCCCCCCCCEEEECCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
LPPFAMHCTFICDDETLQAQARRYRQRLIDWQEAHQNG
CCCHHHEEEEEECCHHHHHHHHHHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; acceptor [C]

Specific reaction: NAD(P)H2 + acceptor = NAD(P) + reduced acceptor [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11053405