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The map label for this gene is iolD1 [H]

Identifier: 150398744

GI number: 150398744

Start: 3765994

End: 3767862

Strand: Reverse

Name: iolD1 [H]

Synonym: Smed_3561

Alternate gene names: 150398744

Gene position: 3767862-3765994 (Counterclockwise)

Preceding gene: 150398745

Following gene: 150398743

Centisome position: 99.63

GC content: 64.85

Gene sequence:

>1869_bases
ATGACCCAGAAAACCGTGCGCCAGGGTACAGTGCGCTTGACCATGTCGCAGGCCGTGGCGCGGTTCCTGACCCGGCAGAT
GACGATCATCGAGGGCGAGCGCGTGCCGATTTTTGGCGGCGTCTTCGCGATTTTCGGTCACGGTAACGTCGCCGGGGTCG
GGGAAGCGCTCTATGCCGTGCGCGAAACCCTTCCGACCTACCGGGCCCAGAACGAGCAGGGCATGGCGAACGCGGCGATC
GCCTTCGCCAAAGCGAGCTTCCGCCGCCGCTTCATGGCATGCACGACGTCCATCGGCCCGGGCGCCCTGAATATGGTGAC
GTCGGCCGCGCTCGCACACGTCAACCGGCTGCCGGTGCTCCTCCTGCCCGGCGACATCTTCGCCAATCGCCGGCCGGACC
CGGTCTTGCAGCAGGTAGAGAGTTTTGGCGACGGGACGATCTCGGCGAACGACTGCTTCCGCCCGGTCTCGCGTTACTTC
GACCGCATCACGCGACCGGAGCAGATCATTCCGGCGCTTCGCCGCGCCATGCAGGTGCTTACAGACCCGGCCGATTGCGG
CCCGGTGACGCTTTCGCTCTGCCAGGACGTTCAGGCGGAAGCCTATGACTACCCGGAATCGTTCTTCGACGAAAAAGTCT
GGGTACCGCGCCGCGTCGAGCCCGATCTCGACGAACTGGCCGCGGCGATCGAGACGCTGAAGGTTGCCAGGAAGCCGATC
ATCATCGCAGGCGGAGGCGTGCTCTATTCGGAAGCGAGCGCCGACCTTGCCGAGTTCGCCGAAAAACACGGCATTCCGGT
CCTCGAGACGCAGGCCGGCAAGTCCGCCCTGCCGCACGCGCATCCGCTGAACATGGGTTCGGTCGGCGTCACCGGTACCT
CTGCCTCCAACGCCTTGGCGGAAGAGGCGGACGTGGTGCTCGCCGTCGGCTCGCGGTTGCAGGATTTCACTACCGGCTCC
TGGGCGCTTTTCAAGAATGAAGCGGTGAAGATCATCGGACTCAACGTCCAGCCCTTCGACGCCGGCAAGCACGATGGACA
GCCTTTGATCGCCGATGCGCGGGCCGGCCTCAACCGCATCTCGGGCGGGCTCGGCAGCTACAGCGCCGACAGCGCCTGGA
CAGAGAAGGCGAGGGCCGGAAAGGCCGAGTGGCTTGCTGCAGCCGACAGGGCGACGGCCACCACCAATGCGGCGCTTCCC
TCCGATGCGCAGGTCATCGGCGCCGTGCAACGCGCCCGTGGCGGGAGGCAAACGACACTGGTCTGCGCCGCCGGCGGGCT
GCCCGGCGAGCTGCACAAGCTCTGGCAGGCGGAGTCTCCAGGCAGCTACCATATGGAATATGGCTTCTCGACCATGGGCT
ATGAGGTCGCTGGCGGCCTTGGCGTGAAACTTGCCAAGCCCGAAAGTGACGTGATCGTCATGGTCGGAGACGGCAGCTAC
ATGATGCTGAACTCCGAGATCGCCTCTTCGGTCATGCTCGGTGCCAAGCTTACGATCGTGCTGCTCGACAATGCCGGCTA
TGGCTGCATCAACAGGTTGCAGATGGGTACGGGCGGCGCCAACTTCAACAACCTGTTGAAGGACACACATCACGTGGCGC
TGCCGCAGATCGACTTCGCCGCCCACGCCGCCGCCATGGGCGCGGTCACCCGAAAGGTGGGATCGATCCCCGAACTCGAA
GCGGCGCTTGCCGAAACGGCAGACGAGGCTCGCACGACCGTCATCGTCATCGATACCGATCCGCTGATCACGACGGAAGC
CGGTGGGCACTGGTGGGACGTCGCGGTCCCGGAGGTTTCGGACCGCGACCAGGTAAAGGCCGCCCGCGAAGATTACGAAA
ATGCCCTCCGGTCACAGCGGTTTGGTTGA

Upstream 100 bases:

>100_bases
GTTCAAGGCACTCGTGGATCTTTGGCTGCAACTGGGCGAGACCAGGGATCTATAGTGGTCAAATAGGAAGCGGCACGGAT
TCGGCCGCGAGGAGGAGACA

Downstream 100 bases:

>100_bases
CCTACTGCGGCCTCCTTCGCGCACCAAAACAAAACCACGAACCAACGGCCTCCGGCAGACAAGACAACAGAGAGCAAGCA
CATGATCCGCTACGGAACCA

Product: thiamine pyrophosphate protein central region

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase 1 [H]

Number of amino acids: Translated: 622; Mature: 621

Protein sequence:

>622_residues
MTQKTVRQGTVRLTMSQAVARFLTRQMTIIEGERVPIFGGVFAIFGHGNVAGVGEALYAVRETLPTYRAQNEQGMANAAI
AFAKASFRRRFMACTTSIGPGALNMVTSAALAHVNRLPVLLLPGDIFANRRPDPVLQQVESFGDGTISANDCFRPVSRYF
DRITRPEQIIPALRRAMQVLTDPADCGPVTLSLCQDVQAEAYDYPESFFDEKVWVPRRVEPDLDELAAAIETLKVARKPI
IIAGGGVLYSEASADLAEFAEKHGIPVLETQAGKSALPHAHPLNMGSVGVTGTSASNALAEEADVVLAVGSRLQDFTTGS
WALFKNEAVKIIGLNVQPFDAGKHDGQPLIADARAGLNRISGGLGSYSADSAWTEKARAGKAEWLAAADRATATTNAALP
SDAQVIGAVQRARGGRQTTLVCAAGGLPGELHKLWQAESPGSYHMEYGFSTMGYEVAGGLGVKLAKPESDVIVMVGDGSY
MMLNSEIASSVMLGAKLTIVLLDNAGYGCINRLQMGTGGANFNNLLKDTHHVALPQIDFAAHAAAMGAVTRKVGSIPELE
AALAETADEARTTVIVIDTDPLITTEAGGHWWDVAVPEVSDRDQVKAAREDYENALRSQRFG

Sequences:

>Translated_622_residues
MTQKTVRQGTVRLTMSQAVARFLTRQMTIIEGERVPIFGGVFAIFGHGNVAGVGEALYAVRETLPTYRAQNEQGMANAAI
AFAKASFRRRFMACTTSIGPGALNMVTSAALAHVNRLPVLLLPGDIFANRRPDPVLQQVESFGDGTISANDCFRPVSRYF
DRITRPEQIIPALRRAMQVLTDPADCGPVTLSLCQDVQAEAYDYPESFFDEKVWVPRRVEPDLDELAAAIETLKVARKPI
IIAGGGVLYSEASADLAEFAEKHGIPVLETQAGKSALPHAHPLNMGSVGVTGTSASNALAEEADVVLAVGSRLQDFTTGS
WALFKNEAVKIIGLNVQPFDAGKHDGQPLIADARAGLNRISGGLGSYSADSAWTEKARAGKAEWLAAADRATATTNAALP
SDAQVIGAVQRARGGRQTTLVCAAGGLPGELHKLWQAESPGSYHMEYGFSTMGYEVAGGLGVKLAKPESDVIVMVGDGSY
MMLNSEIASSVMLGAKLTIVLLDNAGYGCINRLQMGTGGANFNNLLKDTHHVALPQIDFAAHAAAMGAVTRKVGSIPELE
AALAETADEARTTVIVIDTDPLITTEAGGHWWDVAVPEVSDRDQVKAAREDYENALRSQRFG
>Mature_621_residues
TQKTVRQGTVRLTMSQAVARFLTRQMTIIEGERVPIFGGVFAIFGHGNVAGVGEALYAVRETLPTYRAQNEQGMANAAIA
FAKASFRRRFMACTTSIGPGALNMVTSAALAHVNRLPVLLLPGDIFANRRPDPVLQQVESFGDGTISANDCFRPVSRYFD
RITRPEQIIPALRRAMQVLTDPADCGPVTLSLCQDVQAEAYDYPESFFDEKVWVPRRVEPDLDELAAAIETLKVARKPII
IAGGGVLYSEASADLAEFAEKHGIPVLETQAGKSALPHAHPLNMGSVGVTGTSASNALAEEADVVLAVGSRLQDFTTGSW
ALFKNEAVKIIGLNVQPFDAGKHDGQPLIADARAGLNRISGGLGSYSADSAWTEKARAGKAEWLAAADRATATTNAALPS
DAQVIGAVQRARGGRQTTLVCAAGGLPGELHKLWQAESPGSYHMEYGFSTMGYEVAGGLGVKLAKPESDVIVMVGDGSYM
MLNSEIASSVMLGAKLTIVLLDNAGYGCINRLQMGTGGANFNNLLKDTHHVALPQIDFAAHAAAMGAVTRKVGSIPELEA
ALAETADEARTTVIVIDTDPLITTEAGGHWWDVAVPEVSDRDQVKAAREDYENALRSQRFG

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=559, Percent_Identity=26.1180679785331, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1790104, Length=590, Percent_Identity=24.5762711864407, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1786717, Length=441, Percent_Identity=25.8503401360544, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787096, Length=473, Percent_Identity=23.0443974630021, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1788716, Length=509, Percent_Identity=23.9685658153242, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17531301, Length=527, Percent_Identity=24.6679316888046, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531299, Length=527, Percent_Identity=24.6679316888046, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=586, Percent_Identity=24.7440273037543, Blast_Score=134, Evalue=5e-32,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 66219; Mature: 66088

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKTVRQGTVRLTMSQAVARFLTRQMTIIEGERVPIFGGVFAIFGHGNVAGVGEALYAV
CCCCHHHCCCEEEEHHHHHHHHHHHHHHEECCCCCEEECCEEEEEECCCCCHHHHHHHHH
RETLPTYRAQNEQGMANAAIAFAKASFRRRFMACTTSIGPGALNMVTSAALAHVNRLPVL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEE
LLPGDIFANRRPDPVLQQVESFGDGTISANDCFRPVSRYFDRITRPEQIIPALRRAMQVL
EECCHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TDPADCGPVTLSLCQDVQAEAYDYPESFFDEKVWVPRRVEPDLDELAAAIETLKVARKPI
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCE
IIAGGGVLYSEASADLAEFAEKHGIPVLETQAGKSALPHAHPLNMGSVGVTGTSASNALA
EEECCCEEECCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
EEADVVLAVGSRLQDFTTGSWALFKNEAVKIIGLNVQPFDAGKHDGQPLIADARAGLNRI
HHCCEEEECCCHHHHCCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCEEHHHHHHHHHH
SGGLGSYSADSAWTEKARAGKAEWLAAADRATATTNAALPSDAQVIGAVQRARGGRQTTL
CCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
VCAAGGLPGELHKLWQAESPGSYHMEYGFSTMGYEVAGGLGVKLAKPESDVIVMVGDGSY
EEECCCCCHHHHHHHCCCCCCCEEEECCCCCCCEEECCCCCEEEECCCCCEEEEECCCCE
MMLNSEIASSVMLGAKLTIVLLDNAGYGCINRLQMGTGGANFNNLLKDTHHVALPQIDFA
EEEEHHHHHHHHHCCEEEEEEECCCCCCHHHHEECCCCCCCHHHHHHHHHHHCCCCCHHH
AHAAAMGAVTRKVGSIPELEAALAETADEARTTVIVIDTDPLITTEAGGHWWDVAVPEVS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCEEEEEEECCCEEEECCCCCEEEEECCCCC
DRDQVKAAREDYENALRSQRFG
CHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQKTVRQGTVRLTMSQAVARFLTRQMTIIEGERVPIFGGVFAIFGHGNVAGVGEALYAV
CCCHHHCCCEEEEHHHHHHHHHHHHHHEECCCCCEEECCEEEEEECCCCCHHHHHHHHH
RETLPTYRAQNEQGMANAAIAFAKASFRRRFMACTTSIGPGALNMVTSAALAHVNRLPVL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEE
LLPGDIFANRRPDPVLQQVESFGDGTISANDCFRPVSRYFDRITRPEQIIPALRRAMQVL
EECCHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TDPADCGPVTLSLCQDVQAEAYDYPESFFDEKVWVPRRVEPDLDELAAAIETLKVARKPI
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCE
IIAGGGVLYSEASADLAEFAEKHGIPVLETQAGKSALPHAHPLNMGSVGVTGTSASNALA
EEECCCEEECCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
EEADVVLAVGSRLQDFTTGSWALFKNEAVKIIGLNVQPFDAGKHDGQPLIADARAGLNRI
HHCCEEEECCCHHHHCCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCEEHHHHHHHHHH
SGGLGSYSADSAWTEKARAGKAEWLAAADRATATTNAALPSDAQVIGAVQRARGGRQTTL
CCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
VCAAGGLPGELHKLWQAESPGSYHMEYGFSTMGYEVAGGLGVKLAKPESDVIVMVGDGSY
EEECCCCCHHHHHHHCCCCCCCEEEECCCCCCCEEECCCCCEEEECCCCCEEEEECCCCE
MMLNSEIASSVMLGAKLTIVLLDNAGYGCINRLQMGTGGANFNNLLKDTHHVALPQIDFA
EEEEHHHHHHHHHCCEEEEEEECCCCCCHHHHEECCCCCCCHHHHHHHHHHHCCCCCHHH
AHAAAMGAVTRKVGSIPELEAALAETADEARTTVIVIDTDPLITTEAGGHWWDVAVPEVS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCEEEEEEECCCEEEECCCCCEEEEECCCCC
DRDQVKAAREDYENALRSQRFG
CHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA