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The map label for this gene is dnaN [H]

Identifier: 150398727

GI number: 150398727

Start: 3748860

End: 3749978

Strand: Reverse

Name: dnaN [H]

Synonym: Smed_3543

Alternate gene names: 150398727

Gene position: 3749978-3748860 (Counterclockwise)

Preceding gene: 150398733

Following gene: 150398726

Centisome position: 99.16

GC content: 61.57

Gene sequence:

>1119_bases
ATGCGTATTACTCTAGAGCGGTCCAACCTGTTGAAATCGCTGAACCATGTCCACCGTGTGGTGGAACGGCGAAATACGAT
CCCGATCCTTTCGAACGTGCTCTTGCGCTCCGACGGCGCCAGTCTCGAAATGAAGGCGACCGACCTCGACCTGGAGATCA
CCGAGGCGACGCAGGCGCAGGTCGAGCAGGCGGGCGCCACGACCGTTCCGGCGCACCTGCTCTATGACATCGTGCGCAAG
TTGCCCGACGGTTCAGAGGTGCTGCTTGCCACCAATGCGGAGGGCACGGCGATGACGGTCGCCTCCGGCCGCTCCAAATT
TTCGCTGCAGTGCCTGCCCCAATCGGACTTCCCCGACCTGACCGCCGGCACGTTCTCGCATTCCTTCCGCCTGAAGGCGA
CCGATCTGAAGATGCTGATCGACCGCACGCAGTTCGCGATTTCGACGGAAGAGACGCGCTATTATCTGAACGGCATTTTC
GTGCACACGGTCGAGAGCAACGGCGAACTGAAGCTGCGCGCCGTCGCGACCGACGGACACAGGCTCGCCCGTGCTGATGT
CGAAGCCCCGTCCGGCTCCGAGGGCATGCCCGGCATCATCATCCCGCGCAAAACCGTAAGCGAATTGCAAAAATTGCTCG
ACAATCCCGACGTCGTCGTCACAGTCGAGGTCTCGGACGCGAAAATCCGGTTGACGATCGGCTCCATCGTCATGACGTCG
AAGCTGATCGACGGGACCTTCCCCGACTATCAGCGCGTGATCCCCGCCAGCAATGACAAGGAATTGCGCGTCGACTGCCA
GTCCTTCTCGCAGGCCGTCGACCGCGTCTCGACGATCTCGTCCGAACGCGGACGCGCCGTGAAGCTGGCACTCACCGATG
GCCAGATGACGCTGACGGTAAACAACCCGGATTCGGGCAGCGCCACCGAAGAAGTGCCGGTCGGCTACGAAAGTGATCCG
CTCGAGATCGGATTCAACGCAAAGTATCTTCTCGACATCACCGCGCAACTGACCGGCAGCGAGGCGGTCTTCCTGCTCGC
AGATCCCGGTTCGCCGACGCTGGTACGCGACCTGGCCGCGGATGACGCGCTCTACGTCCTAATGCCGATGCGAGTCTAA

Upstream 100 bases:

>100_bases
TGCCCGTCCACATCCTTCCGGCCAAGCGGCCGGGGCACGCAGTTGACGCCGCGGCGGTGACCCTCGCCCGCCCCGGCAAC
GGTTGAAAGCGGTAGCATCC

Downstream 100 bases:

>100_bases
CGGCCTGTCGCAGGTACGGATGAAACCGCCCTTCGGCGCGCTTCATCCTATTTCCCGTCGCGCATTGTTCATGCACTGTC
GCTTTTAGACGCAGCCGGAC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRITLERSNLLKSLNHVHRVVERRNTIPILSNVLLRSDGASLEMKATDLDLEITEATQAQVEQAGATTVPAHLLYDIVRK
LPDGSEVLLATNAEGTAMTVASGRSKFSLQCLPQSDFPDLTAGTFSHSFRLKATDLKMLIDRTQFAISTEETRYYLNGIF
VHTVESNGELKLRAVATDGHRLARADVEAPSGSEGMPGIIIPRKTVSELQKLLDNPDVVVTVEVSDAKIRLTIGSIVMTS
KLIDGTFPDYQRVIPASNDKELRVDCQSFSQAVDRVSTISSERGRAVKLALTDGQMTLTVNNPDSGSATEEVPVGYESDP
LEIGFNAKYLLDITAQLTGSEAVFLLADPGSPTLVRDLAADDALYVLMPMRV

Sequences:

>Translated_372_residues
MRITLERSNLLKSLNHVHRVVERRNTIPILSNVLLRSDGASLEMKATDLDLEITEATQAQVEQAGATTVPAHLLYDIVRK
LPDGSEVLLATNAEGTAMTVASGRSKFSLQCLPQSDFPDLTAGTFSHSFRLKATDLKMLIDRTQFAISTEETRYYLNGIF
VHTVESNGELKLRAVATDGHRLARADVEAPSGSEGMPGIIIPRKTVSELQKLLDNPDVVVTVEVSDAKIRLTIGSIVMTS
KLIDGTFPDYQRVIPASNDKELRVDCQSFSQAVDRVSTISSERGRAVKLALTDGQMTLTVNNPDSGSATEEVPVGYESDP
LEIGFNAKYLLDITAQLTGSEAVFLLADPGSPTLVRDLAADDALYVLMPMRV
>Mature_372_residues
MRITLERSNLLKSLNHVHRVVERRNTIPILSNVLLRSDGASLEMKATDLDLEITEATQAQVEQAGATTVPAHLLYDIVRK
LPDGSEVLLATNAEGTAMTVASGRSKFSLQCLPQSDFPDLTAGTFSHSFRLKATDLKMLIDRTQFAISTEETRYYLNGIF
VHTVESNGELKLRAVATDGHRLARADVEAPSGSEGMPGIIIPRKTVSELQKLLDNPDVVVTVEVSDAKIRLTIGSIVMTS
KLIDGTFPDYQRVIPASNDKELRVDCQSFSQAVDRVSTISSERGRAVKLALTDGQMTLTVNNPDSGSATEEVPVGYESDP
LEIGFNAKYLLDITAQLTGSEAVFLLADPGSPTLVRDLAADDALYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=41.3333333333333, Blast_Score=280, Evalue=1e-76,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40472; Mature: 40472

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITLERSNLLKSLNHVHRVVERRNTIPILSNVLLRSDGASLEMKATDLDLEITEATQAQ
CEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCCEEEEEEECCCEEEEHHHHHH
VEQAGATTVPAHLLYDIVRKLPDGSEVLLATNAEGTAMTVASGRSKFSLQCLPQSDFPDL
HHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCC
TAGTFSHSFRLKATDLKMLIDRTQFAISTEETRYYLNGIFVHTVESNGELKLRAVATDGH
CCCCCCCEEEEEECHHHHHHHHHHEEEECCCCEEEEEEEEEEEEECCCCEEEEEEECCCC
RLARADVEAPSGSEGMPGIIIPRKTVSELQKLLDNPDVVVTVEVSDAKIRLTIGSIVMTS
EEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCEEEEH
KLIDGTFPDYQRVIPASNDKELRVDCQSFSQAVDRVSTISSERGRAVKLALTDGQMTLTV
HHHCCCCCCHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEE
NNPDSGSATEEVPVGYESDPLEIGFNAKYLLDITAQLTGSEAVFLLADPGSPTLVRDLAA
CCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEEEEEECCCCEEEEEECCCCCHHHHHHCC
DDALYVLMPMRV
CCEEEEEEECCC
>Mature Secondary Structure
MRITLERSNLLKSLNHVHRVVERRNTIPILSNVLLRSDGASLEMKATDLDLEITEATQAQ
CEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCCEEEEEEECCCEEEEHHHHHH
VEQAGATTVPAHLLYDIVRKLPDGSEVLLATNAEGTAMTVASGRSKFSLQCLPQSDFPDL
HHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCC
TAGTFSHSFRLKATDLKMLIDRTQFAISTEETRYYLNGIFVHTVESNGELKLRAVATDGH
CCCCCCCEEEEEECHHHHHHHHHHEEEECCCCEEEEEEEEEEEEECCCCEEEEEEECCCC
RLARADVEAPSGSEGMPGIIIPRKTVSELQKLLDNPDVVVTVEVSDAKIRLTIGSIVMTS
EEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCEEEEH
KLIDGTFPDYQRVIPASNDKELRVDCQSFSQAVDRVSTISSERGRAVKLALTDGQMTLTV
HHHCCCCCCHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEE
NNPDSGSATEEVPVGYESDPLEIGFNAKYLLDITAQLTGSEAVFLLADPGSPTLVRDLAA
CCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEEEEEECCCCEEEEEECCCCCHHHHHHCC
DDALYVLMPMRV
CCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]