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The map label for this gene is cysK [H]

Identifier: 150398733

GI number: 150398733

Start: 3755212

End: 3756180

Strand: Reverse

Name: cysK [H]

Synonym: Smed_3549

Alternate gene names: 150398733

Gene position: 3756180-3755212 (Counterclockwise)

Preceding gene: 150398735

Following gene: 150398727

Centisome position: 99.32

GC content: 62.54

Gene sequence:

>969_bases
ATGTCTGAAGCGCGCAAGCCCGGCCGTGGCCGCGTCTTTTCTTCGATCACCGAGACGATCGGCGACACTCCCATCGTGCG
GCTGGACAAGCTCGCCAAGGAAAAGGGCGTGAAAGCAAACCTGCTCGCCAAGCTGGAATTCTTCAACCCCATAGGCTCGG
TGAAGGACCGCATCGGCGTTGCGATGATCGAATCACTTGAGGAGCAGGGCAAGATCACCCCCGGCCGCACAACGCTCGTC
GAGCCGACTTCCGGCAATACGGGCATAGCGCTCGCCTTTGTGGCGGCTGCGAAAGGTTACCGGTTGATCCTCACCATGCC
CGAAACCATGTCCGTCGAGCGGCGCAAGATGCTGGCCCTTCTCGGTGCCGAACTGGTGCTGACGGAGGGTGCCAAGGGCA
TGAAGGGCGCGATCGCCAAGGCCCAGGAACTTACCGAGACCCTTCCCGACGCAATCATTCCGCAGCAGTTCGAAAATCCG
GCCAATCCGGAAATCCATCGCCAGACGACGGCCGAGGAGATCTGGAACGACACGGAAGGCGCGGTTGACATCCTCGTATC
GGGCATCGGCACGGGCGGTACCATTACCGGCGCAGGCCAGGTCTTGAAGGCGCGTAAGCCATCGCTCCGGGTGATCGCCG
TCGAGCCCGAGGAATCTCCGATACTCTCCGGCGGCGCCCCGGGTCCGCACAAAATCCAGGGCATCGGCGCGGGATTCGCA
CCGGCGATCCTCGACACGAGCGTCTATGACGAGGTCGTCACCGTAAATGCCGGCGAGGCCGTTGAAACAGCACGGCTCGT
CGCCAGGCTCGAAGGCGTCCCGGTCGGTATTTCCGCCGGTGCGGCGCTCCAGGCGGCGATCGAAATCGGCCAGCGCGAGG
AAAACGCCGGCAAGAATATTGTGGTGATCATTCCCTCTTTTGCGGAGCGCTACCTGTCGACGGTGTTGTTCGAGGGGTTG
GGAGCGTAG

Upstream 100 bases:

>100_bases
TTTTCCTCCCGCTCCGTCGCTAAGGGGCTTTCGCGCTCTGGAAGGAAATGCACATTGCTCCTATTCTCCGGGCTATAAAT
CAAAACCAAGGGAGAAACCT

Downstream 100 bases:

>100_bases
CGCAAGCACTCAGCGAGCGCCCCTCATCCGGCTGCCGCCACCTTCAAGGCGGAGCGAAGGGACAAGGGGCACCACCTGAG
CCCGTCCTCACCGCTTTGCC

Product: cysteine synthase A

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSEARKPGRGRVFSSITETIGDTPIVRLDKLAKEKGVKANLLAKLEFFNPIGSVKDRIGVAMIESLEEQGKITPGRTTLV
EPTSGNTGIALAFVAAAKGYRLILTMPETMSVERRKMLALLGAELVLTEGAKGMKGAIAKAQELTETLPDAIIPQQFENP
ANPEIHRQTTAEEIWNDTEGAVDILVSGIGTGGTITGAGQVLKARKPSLRVIAVEPEESPILSGGAPGPHKIQGIGAGFA
PAILDTSVYDEVVTVNAGEAVETARLVARLEGVPVGISAGAALQAAIEIGQREENAGKNIVVIIPSFAERYLSTVLFEGL
GA

Sequences:

>Translated_322_residues
MSEARKPGRGRVFSSITETIGDTPIVRLDKLAKEKGVKANLLAKLEFFNPIGSVKDRIGVAMIESLEEQGKITPGRTTLV
EPTSGNTGIALAFVAAAKGYRLILTMPETMSVERRKMLALLGAELVLTEGAKGMKGAIAKAQELTETLPDAIIPQQFENP
ANPEIHRQTTAEEIWNDTEGAVDILVSGIGTGGTITGAGQVLKARKPSLRVIAVEPEESPILSGGAPGPHKIQGIGAGFA
PAILDTSVYDEVVTVNAGEAVETARLVARLEGVPVGISAGAALQAAIEIGQREENAGKNIVVIIPSFAERYLSTVLFEGL
GA
>Mature_321_residues
SEARKPGRGRVFSSITETIGDTPIVRLDKLAKEKGVKANLLAKLEFFNPIGSVKDRIGVAMIESLEEQGKITPGRTTLVE
PTSGNTGIALAFVAAAKGYRLILTMPETMSVERRKMLALLGAELVLTEGAKGMKGAIAKAQELTETLPDAIIPQQFENPA
NPEIHRQTTAEEIWNDTEGAVDILVSGIGTGGTITGAGQVLKARKPSLRVIAVEPEESPILSGGAPGPHKIQGIGAGFAP
AILDTSVYDEVVTVNAGEAVETARLVARLEGVPVGISAGAALQAAIEIGQREENAGKNIVVIIPSFAERYLSTVLFEGLG
A

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=314, Percent_Identity=41.7197452229299, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI295821200, Length=314, Percent_Identity=41.7197452229299, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI4557415, Length=314, Percent_Identity=41.7197452229299, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1788754, Length=319, Percent_Identity=53.9184952978056, Blast_Score=297, Evalue=5e-82,
Organism=Escherichia coli, GI2367138, Length=305, Percent_Identity=39.344262295082, Blast_Score=203, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI115535073, Length=298, Percent_Identity=56.3758389261745, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17535051, Length=303, Percent_Identity=49.1749174917492, Blast_Score=307, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=48.6666666666667, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI32566674, Length=295, Percent_Identity=49.1525423728814, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17561720, Length=303, Percent_Identity=48.1848184818482, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=48.5849056603774, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17534315, Length=329, Percent_Identity=40.1215805471125, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI25147552, Length=321, Percent_Identity=38.006230529595, Blast_Score=180, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI17561716, Length=121, Percent_Identity=47.1074380165289, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321594, Length=328, Percent_Identity=36.5853658536585, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321449, Length=332, Percent_Identity=32.8313253012048, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24643623, Length=320, Percent_Identity=37.1875, Blast_Score=195, Evalue=4e-50,
Organism=Drosophila melanogaster, GI20129101, Length=320, Percent_Identity=37.1875, Blast_Score=195, Evalue=4e-50,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33735; Mature: 33604

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEARKPGRGRVFSSITETIGDTPIVRLDKLAKEKGVKANLLAKLEFFNPIGSVKDRIGV
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHH
AMIESLEEQGKITPGRTTLVEPTSGNTGIALAFVAAAKGYRLILTMPETMSVERRKMLAL
HHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHH
LGAELVLTEGAKGMKGAIAKAQELTETLPDAIIPQQFENPANPEIHRQTTAEEIWNDTEG
HCCHHEEECCCCCHHHHHHHHHHHHHHCCHHCCCHHCCCCCCCCHHHHHHHHHHHCCCCC
AVDILVSGIGTGGTITGAGQVLKARKPSLRVIAVEPEESPILSGGAPGPHKIQGIGAGFA
HHHHHEECCCCCCEECCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCH
PAILDTSVYDEVVTVNAGEAVETARLVARLEGVPVGISAGAALQAAIEIGQREENAGKNI
HHHHHHHHHHCEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEE
VVIIPSFAERYLSTVLFEGLGA
EEEECHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEARKPGRGRVFSSITETIGDTPIVRLDKLAKEKGVKANLLAKLEFFNPIGSVKDRIGV
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHH
AMIESLEEQGKITPGRTTLVEPTSGNTGIALAFVAAAKGYRLILTMPETMSVERRKMLAL
HHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHH
LGAELVLTEGAKGMKGAIAKAQELTETLPDAIIPQQFENPANPEIHRQTTAEEIWNDTEG
HCCHHEEECCCCCHHHHHHHHHHHHHHCCHHCCCHHCCCCCCCCHHHHHHHHHHHCCCCC
AVDILVSGIGTGGTITGAGQVLKARKPSLRVIAVEPEESPILSGGAPGPHKIQGIGAGFA
HHHHHEECCCCCCEECCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCH
PAILDTSVYDEVVTVNAGEAVETARLVARLEGVPVGISAGAALQAAIEIGQREENAGKNI
HHHHHHHHHHCEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEE
VVIIPSFAERYLSTVLFEGLGA
EEEECHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]