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The map label for this gene is yjeA [H]

Identifier: 150398663

GI number: 150398663

Start: 3684236

End: 3685288

Strand: Reverse

Name: yjeA [H]

Synonym: Smed_3477

Alternate gene names: 150398663

Gene position: 3685288-3684236 (Counterclockwise)

Preceding gene: 150398667

Following gene: 150398662

Centisome position: 97.45

GC content: 65.53

Gene sequence:

>1053_bases
ATGCCGCATGCTTCTCCCTGGTGGACCCGCGATGTCCATGCCGACCGGCGCCCTTTCCTGATCGGGCGCAATCGCATCCA
GTCGGCGCTCCGCCGGTTCTTCGACGAACGGGATTTCATCGAGGTCGACACGGCAGCACTGCAGGTGTCCCCGGGTAACG
AGGCCCATCTGCATGCCTTCGCGACGCAGGCGCTTGGACTCGACGGCTCTGTGCAGCCGCTCTACCTCCACACTTCGCCC
GAATTCGCCTGCAAGAAGCTCATTGCAGCCGGCGAACGGCGCATCGCCTGCTTCGCCCATGTCTACCGCAATCGCGAGCG
TGGGGCCCTGCATCATCCGGAGTTCACCATGCTCGAATGGTATCGGGCAGAAGAAAGCTACGAAACGCTGATGCGCGACT
GCGCCGAAATCCTGGCGCTTGCCGCCGAAACCGTTGCGGCGCGGTCTCTCGTTTACGGGGGACGAGAGTGCGATCCCTTC
GCAGAGCCGGAACGGCTCTCGGTCGCCGAAGCATTCTCGCGCTTCGCCGGGATCGACCTCCTCGGCTCCATCGCAAAGGA
CGGTTCCACGGACCGCGAAAACCTCGCGGCGGCCATGCGGAAGGCAGGCTTGCGTGTCGCGCGCGACGATGGCTGGGCCG
ACCTCTTCAGCCGCGTGCTCGTCGAAAAGATCGAGCCGAATCTCGGCTTCGGGCGCGCCACGATCCTCGACGAATATCCG
GTTTCCGAAGCAGCGCTTGCGCGGCGCGCAGCGCGCGACCCGCGGGTCGCGGAGCGTTTCGAGCTCTATGCCTGTGGCGT
CGAGCTCGCAAACGCCTTCGGCGAACTGACCGACGCTGCCGAGCAACGTCGCCGCTTCGCGCTCGAGATGGAGGAAAAGG
CGCGGGTCTATGGCGAGACCTACCCGATCGACGACGACTTCCTTGCGGCGCTCTCCAGCATGCCCGAGGCGAGCGGCATT
GCGCTTGGTTTCGATCGCCTGGTCATGCTCGCGACGGGGGCTTCCCGCATCGACCAGGTCCTCTGGGCTCCGGTCGCGGA
GGTCACGCCATGA

Upstream 100 bases:

>100_bases
GACGCGAGAGTTCAGTTTTCGCGCCGCCACTACCCTAATTTCGCGCAAATAGCCAGCCTGACCGGCTTCCGCGCGGAAGA
AAGCCTGGATCGAACCGATC

Downstream 100 bases:

>100_bases
CCGCGGAGCGCACGATCCGGACGCCGCGTGATCTCGTCGAAGCGGGCCTCATCGGCCGAGAGCGGGAGGAGGCGATTTCA
CGCGTTACGGCAAGCTATGC

Product: lysine--tRNA ligase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MPHASPWWTRDVHADRRPFLIGRNRIQSALRRFFDERDFIEVDTAALQVSPGNEAHLHAFATQALGLDGSVQPLYLHTSP
EFACKKLIAAGERRIACFAHVYRNRERGALHHPEFTMLEWYRAEESYETLMRDCAEILALAAETVAARSLVYGGRECDPF
AEPERLSVAEAFSRFAGIDLLGSIAKDGSTDRENLAAAMRKAGLRVARDDGWADLFSRVLVEKIEPNLGFGRATILDEYP
VSEAALARRAARDPRVAERFELYACGVELANAFGELTDAAEQRRRFALEMEEKARVYGETYPIDDDFLAALSSMPEASGI
ALGFDRLVMLATGASRIDQVLWAPVAEVTP

Sequences:

>Translated_350_residues
MPHASPWWTRDVHADRRPFLIGRNRIQSALRRFFDERDFIEVDTAALQVSPGNEAHLHAFATQALGLDGSVQPLYLHTSP
EFACKKLIAAGERRIACFAHVYRNRERGALHHPEFTMLEWYRAEESYETLMRDCAEILALAAETVAARSLVYGGRECDPF
AEPERLSVAEAFSRFAGIDLLGSIAKDGSTDRENLAAAMRKAGLRVARDDGWADLFSRVLVEKIEPNLGFGRATILDEYP
VSEAALARRAARDPRVAERFELYACGVELANAFGELTDAAEQRRRFALEMEEKARVYGETYPIDDDFLAALSSMPEASGI
ALGFDRLVMLATGASRIDQVLWAPVAEVTP
>Mature_349_residues
PHASPWWTRDVHADRRPFLIGRNRIQSALRRFFDERDFIEVDTAALQVSPGNEAHLHAFATQALGLDGSVQPLYLHTSPE
FACKKLIAAGERRIACFAHVYRNRERGALHHPEFTMLEWYRAEESYETLMRDCAEILALAAETVAARSLVYGGRECDPFA
EPERLSVAEAFSRFAGIDLLGSIAKDGSTDRENLAAAMRKAGLRVARDDGWADLFSRVLVEKIEPNLGFGRATILDEYPV
SEAALARRAARDPRVAERFELYACGVELANAFGELTDAAEQRRRFALEMEEKARVYGETYPIDDDFLAALSSMPEASGIA
LGFDRLVMLATGASRIDQVLWAPVAEVTP

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=338, Percent_Identity=27.2189349112426, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI5031815, Length=338, Percent_Identity=27.2189349112426, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI87082379, Length=326, Percent_Identity=37.1165644171779, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1790571, Length=337, Percent_Identity=32.0474777448071, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789256, Length=330, Percent_Identity=31.5151515151515, Blast_Score=145, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI71994340, Length=336, Percent_Identity=28.8690476190476, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17535927, Length=337, Percent_Identity=29.3768545994065, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17535925, Length=336, Percent_Identity=28.8690476190476, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6320242, Length=349, Percent_Identity=27.5071633237822, Blast_Score=116, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24640851, Length=340, Percent_Identity=26.1764705882353, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24640849, Length=340, Percent_Identity=26.1764705882353, Blast_Score=104, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 39007; Mature: 38876

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHASPWWTRDVHADRRPFLIGRNRIQSALRRFFDERDFIEVDTAALQVSPGNEAHLHAF
CCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHH
ATQALGLDGSVQPLYLHTSPEFACKKLIAAGERRIACFAHVYRNRERGALHHPEFTMLEW
HHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHH
YRAEESYETLMRDCAEILALAAETVAARSLVYGGRECDPFAEPERLSVAEAFSRFAGIDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LGSIAKDGSTDRENLAAAMRKAGLRVARDDGWADLFSRVLVEKIEPNLGFGRATILDEYP
HHHHHCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCC
VSEAALARRAARDPRVAERFELYACGVELANAFGELTDAAEQRRRFALEMEEKARVYGET
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YPIDDDFLAALSSMPEASGIALGFDRLVMLATGASRIDQVLWAPVAEVTP
CCCCHHHHHHHHCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
PHASPWWTRDVHADRRPFLIGRNRIQSALRRFFDERDFIEVDTAALQVSPGNEAHLHAF
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHH
ATQALGLDGSVQPLYLHTSPEFACKKLIAAGERRIACFAHVYRNRERGALHHPEFTMLEW
HHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHH
YRAEESYETLMRDCAEILALAAETVAARSLVYGGRECDPFAEPERLSVAEAFSRFAGIDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LGSIAKDGSTDRENLAAAMRKAGLRVARDDGWADLFSRVLVEKIEPNLGFGRATILDEYP
HHHHHCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCC
VSEAALARRAARDPRVAERFELYACGVELANAFGELTDAAEQRRRFALEMEEKARVYGET
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YPIDDDFLAALSSMPEASGIALGFDRLVMLATGASRIDQVLWAPVAEVTP
CCCCHHHHHHHHCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA