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The map label for this gene is hisH [H]

Identifier: 150398453

GI number: 150398453

Start: 3441048

End: 3441698

Strand: Reverse

Name: hisH [H]

Synonym: Smed_3261

Alternate gene names: 150398453

Gene position: 3441698-3441048 (Counterclockwise)

Preceding gene: 150398454

Following gene: 150398452

Centisome position: 91.0

GC content: 63.44

Gene sequence:

>651_bases
ATGCGGGTCGCCATCATCGATTATGGATCGGGCAATCTGCGCTCGGCCACCAAGGCCTTCGAACGCGCCGCCCGCGAAGC
CGGCATCTCAGCAGAAATCGACCTCACCGACAGACCGGAACGGGTGGTTTCGGCCGACCGCATCGTGCTGCCGGGCGTCG
GCGCCTATGCAGATTGCCGCCGGGGCCTTGCCGCCGTCGACGGTATGCAAGACGCGCTGACGGAAGCCGTTGAAAAGGCC
GGCCGACCCTTCTTCGGCATCTGCGTCGGTATGCAGCTCATGTCGTCGCGAGGGCTTGAAAAGACCGTGACCGAGGGCTT
TGACTGGATCGCCGGGGATGTCGTTGAGATGACGCCCGAAGATCCCTCGCTCAAGATTCCGCAAATCGGCTGGAACACGC
TCGATCTCAGGCGCCCGCACGCGCTTTTCCAGGGCATTGCGACCGGCGAGAGCGGGTTGCACGCCTATTTCGTGCATTCC
TACCATCTTGCGGCAGAGTGCGCCGAGGACGTGGTGGCCGAGGCCGATTATGGCGGCCCGGTGACGGCTTTCGTCGCACG
CGACAACAAGGCAGGCTCGCAGTTCCATCCGGAAAAGAGCCAGGCGCTCGGCCTCGCCCTTATTTCGAATTTCCTGCGCT
GGAAGCCCTGA

Upstream 100 bases:

>100_bases
AAAGCCATGCGAAGCCATTGCCGTCGATCGATCGGTCCGGCACGGGACGCCTGGGCGACACAGCCGGGCTCGGCTTCTTT
CAATCCTATGGAGAACGCTG

Downstream 100 bases:

>100_bases
AGCTGCAGCGTCATTTTTCTGATCGGACGAACAATGATCCTCTTTCCCGCAATCGACCTCAAAGACGGGCAATGCGTGCG
CCTGAAGCTCGGCGACATGG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MRVAIIDYGSGNLRSATKAFERAAREAGISAEIDLTDRPERVVSADRIVLPGVGAYADCRRGLAAVDGMQDALTEAVEKA
GRPFFGICVGMQLMSSRGLEKTVTEGFDWIAGDVVEMTPEDPSLKIPQIGWNTLDLRRPHALFQGIATGESGLHAYFVHS
YHLAAECAEDVVAEADYGGPVTAFVARDNKAGSQFHPEKSQALGLALISNFLRWKP

Sequences:

>Translated_216_residues
MRVAIIDYGSGNLRSATKAFERAAREAGISAEIDLTDRPERVVSADRIVLPGVGAYADCRRGLAAVDGMQDALTEAVEKA
GRPFFGICVGMQLMSSRGLEKTVTEGFDWIAGDVVEMTPEDPSLKIPQIGWNTLDLRRPHALFQGIATGESGLHAYFVHS
YHLAAECAEDVVAEADYGGPVTAFVARDNKAGSQFHPEKSQALGLALISNFLRWKP
>Mature_216_residues
MRVAIIDYGSGNLRSATKAFERAAREAGISAEIDLTDRPERVVSADRIVLPGVGAYADCRRGLAAVDGMQDALTEAVEKA
GRPFFGICVGMQLMSSRGLEKTVTEGFDWIAGDVVEMTPEDPSLKIPQIGWNTLDLRRPHALFQGIATGESGLHAYFVHS
YHLAAECAEDVVAEADYGGPVTAFVARDNKAGSQFHPEKSQALGLALISNFLRWKP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=213, Percent_Identity=38.4976525821596, Blast_Score=126, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=224, Percent_Identity=32.5892857142857, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23322; Mature: 23322

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAIIDYGSGNLRSATKAFERAAREAGISAEIDLTDRPERVVSADRIVLPGVGAYADCR
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCEEEECCCCCHHHHH
RGLAAVDGMQDALTEAVEKAGRPFFGICVGMQLMSSRGLEKTVTEGFDWIAGDVVEMTPE
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHCCHHHCCCEEEECCC
DPSLKIPQIGWNTLDLRRPHALFQGIATGESGLHAYFVHSYHLAAECAEDVVAEADYGGP
CCCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCC
VTAFVARDNKAGSQFHPEKSQALGLALISNFLRWKP
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVAIIDYGSGNLRSATKAFERAAREAGISAEIDLTDRPERVVSADRIVLPGVGAYADCR
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCEEEECCCCCHHHHH
RGLAAVDGMQDALTEAVEKAGRPFFGICVGMQLMSSRGLEKTVTEGFDWIAGDVVEMTPE
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHCCHHHCCCEEEECCC
DPSLKIPQIGWNTLDLRRPHALFQGIATGESGLHAYFVHSYHLAAECAEDVVAEADYGGP
CCCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCC
VTAFVARDNKAGSQFHPEKSQALGLALISNFLRWKP
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]