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The map label for this gene is hisA

Identifier: 150398452

GI number: 150398452

Start: 3440268

End: 3441014

Strand: Reverse

Name: hisA

Synonym: Smed_3260

Alternate gene names: 150398452

Gene position: 3441014-3440268 (Counterclockwise)

Preceding gene: 150398453

Following gene: 150398451

Centisome position: 90.99

GC content: 63.86

Gene sequence:

>747_bases
ATGATCCTCTTTCCCGCAATCGACCTCAAAGACGGGCAATGCGTGCGCCTGAAGCTCGGCGACATGGAGCAGGCGACGGT
CTATAATTCCGACCCGGCCGCGCAGGCCCGCGCTTTTGAGGAACAGGGCTTCGAATGGCTGCACGTGGTCGACCTGAACG
GGGCATTTGCCGGCGAGACGGTCAACGGTGATGCTGTCGACGCCATCCTCAGGGCAACGAAGAACCCGGTGCAGCTCGGC
GGCGGAATCCGCACGCTCGACCATATAGAGAACTGGCTTGCGCGCGGGCTCAAGCGCGTCATCCTCGGCACCGTCGCAGT
GCGCGACCCGGCGCTGGTGATCGAAGCCTGCCGCAAATTCCCGGGCAGGATCGCCGTGGGCATCGATGCGAAGGGCGGCA
AGGTCGCGGTCGAAGGCTGGGCTGAGGCTTCCGAACTCGGCGTGATCGAGCTCGCGCGGAGATTCGAAGGCGCCGGCGTG
GCGGCGATCATCTATACCGATATCGACCGCGACGGCATCCTGACAGGCATCAACTGGGCCGCGACGCTCGAACTCTCCGA
TGCGGTTTCGATTCCCGTCATCGCCTCGGGCGGACTCGCCTCGCTGGACGATATCCGCCGCATGACCGAGCCGGAAGCGC
AAAAACTCGAAGGGGCAATCTCCGGCCGGGCGCTTTATGACGGTCGAATCGATCCGAAGGAAGCGCTCGATCTGATCCGC
GAAGCCAGGAAGGGGATGATCCGATGA

Upstream 100 bases:

>100_bases
CCATCCGGAAAAGAGCCAGGCGCTCGGCCTCGCCCTTATTTCGAATTTCCTGCGCTGGAAGCCCTGAAGCTGCAGCGTCA
TTTTTCTGATCGGACGAACA

Downstream 100 bases:

>100_bases
CGCTGAAAGCCCGTGTGATCCCCTGCCTCGACGTCAAGGACGGCCGTGTCGTCAAAGGCGTCAATTTCGTCGATCTCATC
GATGCCGGCGATCCGGTGGA

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MILFPAIDLKDGQCVRLKLGDMEQATVYNSDPAAQARAFEEQGFEWLHVVDLNGAFAGETVNGDAVDAILRATKNPVQLG
GGIRTLDHIENWLARGLKRVILGTVAVRDPALVIEACRKFPGRIAVGIDAKGGKVAVEGWAEASELGVIELARRFEGAGV
AAIIYTDIDRDGILTGINWAATLELSDAVSIPVIASGGLASLDDIRRMTEPEAQKLEGAISGRALYDGRIDPKEALDLIR
EARKGMIR

Sequences:

>Translated_248_residues
MILFPAIDLKDGQCVRLKLGDMEQATVYNSDPAAQARAFEEQGFEWLHVVDLNGAFAGETVNGDAVDAILRATKNPVQLG
GGIRTLDHIENWLARGLKRVILGTVAVRDPALVIEACRKFPGRIAVGIDAKGGKVAVEGWAEASELGVIELARRFEGAGV
AAIIYTDIDRDGILTGINWAATLELSDAVSIPVIASGGLASLDDIRRMTEPEAQKLEGAISGRALYDGRIDPKEALDLIR
EARKGMIR
>Mature_248_residues
MILFPAIDLKDGQCVRLKLGDMEQATVYNSDPAAQARAFEEQGFEWLHVVDLNGAFAGETVNGDAVDAILRATKNPVQLG
GGIRTLDHIENWLARGLKRVILGTVAVRDPALVIEACRKFPGRIAVGIDAKGGKVAVEGWAEASELGVIELARRFEGAGV
AAIIYTDIDRDGILTGINWAATLELSDAVSIPVIASGGLASLDDIRRMTEPEAQKLEGAISGRALYDGRIDPKEALDLIR
EARKGMIR

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=33.1967213114754, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788336, Length=212, Percent_Identity=23.1132075471698, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_SINMW (A6UEK4)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001328919.1
- ProteinModelPortal:   A6UEK4
- SMR:   A6UEK4
- STRING:   A6UEK4
- GeneID:   5324139
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_3260
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- BioCyc:   SMED366394:SMED_3260-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26598; Mature: 26598

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILFPAIDLKDGQCVRLKLGDMEQATVYNSDPAAQARAFEEQGFEWLHVVDLNGAFAGET
CEEECEEECCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCCCC
VNGDAVDAILRATKNPVQLGGGIRTLDHIENWLARGLKRVILGTVAVRDPALVIEACRKF
CCCHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
PGRIAVGIDAKGGKVAVEGWAEASELGVIELARRFEGAGVAAIIYTDIDRDGILTGINWA
CCEEEEEEECCCCEEEEECCCCCHHCHHHHHHHHCCCCCEEEEEEEECCCCCEEECCCEE
ATLELSDAVSIPVIASGGLASLDDIRRMTEPEAQKLEGAISGRALYDGRIDPKEALDLIR
EEEEECCCCCCCEEECCCCCCHHHHHHHCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHH
EARKGMIR
HHHHCCCC
>Mature Secondary Structure
MILFPAIDLKDGQCVRLKLGDMEQATVYNSDPAAQARAFEEQGFEWLHVVDLNGAFAGET
CEEECEEECCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCCCC
VNGDAVDAILRATKNPVQLGGGIRTLDHIENWLARGLKRVILGTVAVRDPALVIEACRKF
CCCHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
PGRIAVGIDAKGGKVAVEGWAEASELGVIELARRFEGAGVAAIIYTDIDRDGILTGINWA
CCEEEEEEECCCCEEEEECCCCCHHCHHHHHHHHCCCCCEEEEEEEECCCCCEEECCCEE
ATLELSDAVSIPVIASGGLASLDDIRRMTEPEAQKLEGAISGRALYDGRIDPKEALDLIR
EEEEECCCCCCCEEECCCCCCHHHHHHHCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHH
EARKGMIR
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA