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The map label for this gene is rho [H]

Identifier: 150398398

GI number: 150398398

Start: 3381576

End: 3382841

Strand: Reverse

Name: rho [H]

Synonym: Smed_3206

Alternate gene names: 150398398

Gene position: 3382841-3381576 (Counterclockwise)

Preceding gene: 150398399

Following gene: 150398397

Centisome position: 89.45

GC content: 58.29

Gene sequence:

>1266_bases
ATGGCTGAAATGAAGCTTCAAGAACTTAAGAACAAGACTCCGACCGACCTTCTGGCCCTTGCCGAAGAGCTCGAGGTGGA
GAATGCCAGCACGATGCGCAAGCAGGAGCTGATGTTCGCGATCCTCAAAATGCTGGCTCAGCAGGAGATCGAGATCATCG
GCGAGGGTGTCGTCGAGGTCCTGCAGGACGGATTTGGCTTCCTGCGCTCGGCGAATGCGAATTATCTTCCAGGCCCGGAC
GATATCTACATTTCGCCTTCCCAGATCCGCCGCTTCTCGCTGAAGACCGGCGACACGGTCGAGGGTCCGATCCGCGGACC
CAAGGAAGGCGAGCGCTATTTCGCACTGCTGAAGGTCAACACGATCAATTTCGACGATCCGGAGAAGATCCGCCACAAGG
TGCACTTCGACAACCTGACGCCGCTCTATCCGAACGAGCGCTTCAAGATGGAACTCGAGGTCCCGACCTCCAAGGACCTG
TCGGCACGTGTCATAGATCTGGTCGCGCCGCTCGGCAAAGGCCAGCGTGGCTTGATCGTTGCGCCGCCTCGTACCGGTAA
AACGGTTCTTCTGCAGAATATCGCGCATTCGATCACGGCGAATCACCCGGAGTGCTATCTGATCGTCCTCCTGATCGACG
AGCGGCCGGAGGAAGTCACGGACATGCAGCGTTCGGTCAAGGGCGAGGTGGTATCCTCGACCTTCGACGAGCCGGCAACA
CGCCACGTACAGGTCGCGGAAATGGTGATCGAGAAGGCAAAGCGCCTCGTGGAACATGGGCGCGATGTGGTGATCCTGCT
CGATTCGATCACTCGTCTCGGCCGCGCATACAACACAGTCGTTCCCTCATCGGGCAAGGTCCTGACCGGTGGTGTCGACG
CCAATGCGCTGCAGCGCCCCAAGCGCTTCTTCGGCGCGGCTCGTAACATCGAGGAAGGCGGGTCGCTGACGATCATTGCG
ACGGCGCTGATCGACACCGGCAGCCGAATGGATGAAGTGATCTTCGAAGAGTTCAAGGGCACCGGCAACTCGGAAATCGT
TCTGGACCGCAAGGTGGCAGACAAGCGCATCTTCCCGTCAATGGATATTCTCAAATCCGGCACGCGTAAGGAAGATCTCC
TGGTCCCGCGTCAGGACCTGCAGAAGATCTTCGTTCTTCGCCGCATTCTCGCGCCGATGGGGACGACCGATGCGATCGAA
TTCCTCATCGACAAGCTCAAGCAGACGAAGACGAACGGCGACTTCTTCGACTCGATGAATACATAG

Upstream 100 bases:

>100_bases
CCCTTGGCCAGTGACGAAGTTTTAGAGCCTGCCTCTTCCCCCTTGCAGCTCCCTTACAAATCGCGCCGGCTCCCAATCCC
AGATCAAGCACGGACCCTTC

Downstream 100 bases:

>100_bases
CCGCGGGAGAGCGGTTTAAGCCCCCACAGCGGCGCATGAACCACAGGCGGCAGCTTTTGCCGCCTGCCGGTACCTCTCCC
CATTACGGCGGGGAGACGAA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MAEMKLQELKNKTPTDLLALAEELEVENASTMRKQELMFAILKMLAQQEIEIIGEGVVEVLQDGFGFLRSANANYLPGPD
DIYISPSQIRRFSLKTGDTVEGPIRGPKEGERYFALLKVNTINFDDPEKIRHKVHFDNLTPLYPNERFKMELEVPTSKDL
SARVIDLVAPLGKGQRGLIVAPPRTGKTVLLQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPAT
RHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIA
TALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPSMDILKSGTRKEDLLVPRQDLQKIFVLRRILAPMGTTDAIE
FLIDKLKQTKTNGDFFDSMNT

Sequences:

>Translated_421_residues
MAEMKLQELKNKTPTDLLALAEELEVENASTMRKQELMFAILKMLAQQEIEIIGEGVVEVLQDGFGFLRSANANYLPGPD
DIYISPSQIRRFSLKTGDTVEGPIRGPKEGERYFALLKVNTINFDDPEKIRHKVHFDNLTPLYPNERFKMELEVPTSKDL
SARVIDLVAPLGKGQRGLIVAPPRTGKTVLLQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPAT
RHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIA
TALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPSMDILKSGTRKEDLLVPRQDLQKIFVLRRILAPMGTTDAIE
FLIDKLKQTKTNGDFFDSMNT
>Mature_420_residues
AEMKLQELKNKTPTDLLALAEELEVENASTMRKQELMFAILKMLAQQEIEIIGEGVVEVLQDGFGFLRSANANYLPGPDD
IYISPSQIRRFSLKTGDTVEGPIRGPKEGERYFALLKVNTINFDDPEKIRHKVHFDNLTPLYPNERFKMELEVPTSKDLS
ARVIDLVAPLGKGQRGLIVAPPRTGKTVLLQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPATR
HVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIAT
ALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPSMDILKSGTRKEDLLVPRQDLQKIFVLRRILAPMGTTDAIEF
LIDKLKQTKTNGDFFDSMNT

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=70.1923076923077, Blast_Score=617, Evalue=1e-178,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47042; Mature: 46911

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEMKLQELKNKTPTDLLALAEELEVENASTMRKQELMFAILKMLAQQEIEIIGEGVVEV
CCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQDGFGFLRSANANYLPGPDDIYISPSQIRRFSLKTGDTVEGPIRGPKEGERYFALLKVN
HHHHHHHHHCCCCCCCCCCCCEEECHHHHHEEECCCCCCCCCCCCCCCCCCEEEEEEEEE
TINFDDPEKIRHKVHFDNLTPLYPNERFKMELEVPTSKDLSARVIDLVAPLGKGQRGLIV
EECCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEE
APPRTGKTVLLQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPAT
ECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHCCCCHH
RHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRP
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHH
KRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPS
HHHHHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHCCCC
MDILKSGTRKEDLLVPRQDLQKIFVLRRILAPMGTTDAIEFLIDKLKQTKTNGDFFDSMN
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
T
C
>Mature Secondary Structure 
AEMKLQELKNKTPTDLLALAEELEVENASTMRKQELMFAILKMLAQQEIEIIGEGVVEV
CCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQDGFGFLRSANANYLPGPDDIYISPSQIRRFSLKTGDTVEGPIRGPKEGERYFALLKVN
HHHHHHHHHCCCCCCCCCCCCEEECHHHHHEEECCCCCCCCCCCCCCCCCCEEEEEEEEE
TINFDDPEKIRHKVHFDNLTPLYPNERFKMELEVPTSKDLSARVIDLVAPLGKGQRGLIV
EECCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEE
APPRTGKTVLLQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPAT
ECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHCCCCHH
RHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRP
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHH
KRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPS
HHHHHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHCCCC
MDILKSGTRKEDLLVPRQDLQKIFVLRRILAPMGTTDAIEFLIDKLKQTKTNGDFFDSMN
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606169 [H]