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The map label for this gene is trmE

Identifier: 150398397

GI number: 150398397

Start: 3379862

End: 3381184

Strand: Reverse

Name: trmE

Synonym: Smed_3205

Alternate gene names: 150398397

Gene position: 3381184-3379862 (Counterclockwise)

Preceding gene: 150398398

Following gene: 150398396

Centisome position: 89.4

GC content: 60.24

Gene sequence:

>1323_bases
ATGCAATTCACCGATACGATCTACGCGCTGTCGAGCGGCTCGCCACCGGCTGGGGTCGCATTGATCCGCGTCAGTGGTCC
GGCAACAGCCGACGCCCTGGCGCGATTGTGTGGCCCACTGCCGCCGGCGCGTGTTGCGACCTTGAGAACGATTCGGACTC
GAAACAGTGACATCCTGGACAGCGGCCTCGTCCTCTACTTCCCCGGTCCGGCCTCTTTCACAGGTGAGGATTGCTGCGAG
TTGCAGGTTCACGGCGGAAGAGCCGTGGTGAGTGCCATTCTGGACGAGCTGGCCGCGATGGATGGGCTGAGGCATGCGGA
AGCGGGAGAATTTGCGCGGCGAGCTTTTCAGAACGGCAAGCTGGATCTGGTCGAAGTGGAGGGACTGGCGGATCTGATTG
CGGCCGAGACGGAGATGCAGCGGCGACTTGCGGTTGAGCAGTCCGGAGGAGGCCAGTCGGCTCTTTACGCGGGCTGGGCC
CGACGCCTGACCCATGCACGGGCCATGATCGAGGCGGAGCTCGATTTCGCCGACGAGGACGATATTCCGGGTTCCGTCAG
TGCGGCGATCTGGACCGATATCGGCAGGCTGCGAGAAGAGATAGATGAGCACATCGCCCGCGCTGGTGTCGCCGAGATCA
TTCGTGACGGGCTCAAAATCGTTATTGCCGGCGAACCCAATGCCGGCAAGTCCAGCCTGCTCAACGCTTTGGCGCAAAGG
GATATAGCTATCGTTACGGAGATTGCCGGCACGACACGGGATGTTCTGTCTGTCGACCTTTCGTTAGCCGGATTCTCAGT
CAAGCTCTTCGACACGGCAGGCCTGCGCGAAACGGATGAAGTGGTTGAGCGGGAAGGGATTCGGCGTGCCCGCCAGGTTA
TCGCCGATGCCGATCTTGTGCTCCTGCTATCGGAAAAGCCGAGTGATTTCCGCTTGGACGAATGGCAGCCGGGAAAGAGT
GTCCCGGTTATACGCGTCGCTACCAAAGTTGATCGGCCGATGCCGCGTTGGAAGACTTCCGAGGCGGACGTGTTTCTCTC
GACCAGAACCGGCGAGGGTATGGATAAGTTGCTGGCTATGCTTCAGGCACATCTCCCTGATCTGGCGGGCAATACGGCCC
TGTCCATGCCATCGCGGAGGCGTCATGTCGATTGCTTGCGGCAGGCCGGCGCAGCGCTGGTGCGTAGCATGGAGGCCAGA
GAGCTCGAACTTCAGGCTGAACAGCTACGGCAAGCCGGTGACGCGCTGGGCAGAATAACAGGACGCGTCGATGTAGAGAA
ACTTTTAGACGTGATATTCTCGGAGTTCTGCATCGGAAAATAG

Upstream 100 bases:

>100_bases
CGCATCCTGCCGGCTACGCCCGATTCCGATATTGACGAAGCCGAGACGGCAGGCCCGGCCTGCGGCTTAGCGCCGTTCGC
CATTGATGAACCGGTAAGCG

Downstream 100 bases:

>100_bases
ACTATTACCTGTGAGAGCGAATACAACGCAGGCGAGTCATTGAGCCGTTTCACGTGAAACGTCCAGTCGAAGATATGGAA
CTTTTGATCAAAATGTGCGT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MQFTDTIYALSSGSPPAGVALIRVSGPATADALARLCGPLPPARVATLRTIRTRNSDILDSGLVLYFPGPASFTGEDCCE
LQVHGGRAVVSAILDELAAMDGLRHAEAGEFARRAFQNGKLDLVEVEGLADLIAAETEMQRRLAVEQSGGGQSALYAGWA
RRLTHARAMIEAELDFADEDDIPGSVSAAIWTDIGRLREEIDEHIARAGVAEIIRDGLKIVIAGEPNAGKSSLLNALAQR
DIAIVTEIAGTTRDVLSVDLSLAGFSVKLFDTAGLRETDEVVEREGIRRARQVIADADLVLLLSEKPSDFRLDEWQPGKS
VPVIRVATKVDRPMPRWKTSEADVFLSTRTGEGMDKLLAMLQAHLPDLAGNTALSMPSRRRHVDCLRQAGAALVRSMEAR
ELELQAEQLRQAGDALGRITGRVDVEKLLDVIFSEFCIGK

Sequences:

>Translated_440_residues
MQFTDTIYALSSGSPPAGVALIRVSGPATADALARLCGPLPPARVATLRTIRTRNSDILDSGLVLYFPGPASFTGEDCCE
LQVHGGRAVVSAILDELAAMDGLRHAEAGEFARRAFQNGKLDLVEVEGLADLIAAETEMQRRLAVEQSGGGQSALYAGWA
RRLTHARAMIEAELDFADEDDIPGSVSAAIWTDIGRLREEIDEHIARAGVAEIIRDGLKIVIAGEPNAGKSSLLNALAQR
DIAIVTEIAGTTRDVLSVDLSLAGFSVKLFDTAGLRETDEVVEREGIRRARQVIADADLVLLLSEKPSDFRLDEWQPGKS
VPVIRVATKVDRPMPRWKTSEADVFLSTRTGEGMDKLLAMLQAHLPDLAGNTALSMPSRRRHVDCLRQAGAALVRSMEAR
ELELQAEQLRQAGDALGRITGRVDVEKLLDVIFSEFCIGK
>Mature_440_residues
MQFTDTIYALSSGSPPAGVALIRVSGPATADALARLCGPLPPARVATLRTIRTRNSDILDSGLVLYFPGPASFTGEDCCE
LQVHGGRAVVSAILDELAAMDGLRHAEAGEFARRAFQNGKLDLVEVEGLADLIAAETEMQRRLAVEQSGGGQSALYAGWA
RRLTHARAMIEAELDFADEDDIPGSVSAAIWTDIGRLREEIDEHIARAGVAEIIRDGLKIVIAGEPNAGKSSLLNALAQR
DIAIVTEIAGTTRDVLSVDLSLAGFSVKLFDTAGLRETDEVVEREGIRRARQVIADADLVLLLSEKPSDFRLDEWQPGKS
VPVIRVATKVDRPMPRWKTSEADVFLSTRTGEGMDKLLAMLQAHLPDLAGNTALSMPSRRRHVDCLRQAGAALVRSMEAR
ELELQAEQLRQAGDALGRITGRVDVEKLLDVIFSEFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=458, Percent_Identity=44.3231441048035, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI193082993, Length=446, Percent_Identity=44.6188340807175, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI193082988, Length=490, Percent_Identity=41.6326530612245, Blast_Score=305, Evalue=8e-83,
Organism=Escherichia coli, GI2367268, Length=460, Percent_Identity=40.4347826086956, Blast_Score=269, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17507259, Length=447, Percent_Identity=32.8859060402685, Blast_Score=209, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=33.6734693877551, Blast_Score=253, Evalue=5e-68,
Organism=Drosophila melanogaster, GI45550813, Length=471, Percent_Identity=40.1273885350318, Blast_Score=312, Evalue=3e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_SINMW (A6UEE9)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001328864.1
- ProteinModelPortal:   A6UEE9
- SMR:   A6UEE9
- STRING:   A6UEE9
- GeneID:   5324084
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_3205
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   SMED366394:SMED_3205-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 47507; Mature: 47507

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 80-80 BINDING 120-120 BINDING 440-440

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFTDTIYALSSGSPPAGVALIRVSGPATADALARLCGPLPPARVATLRTIRTRNSDILD
CCCCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHC
SGLVLYFPGPASFTGEDCCELQVHGGRAVVSAILDELAAMDGLRHAEAGEFARRAFQNGK
CCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
LDLVEVEGLADLIAAETEMQRRLAVEQSGGGQSALYAGWARRLTHARAMIEAELDFADED
EEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DIPGSVSAAIWTDIGRLREEIDEHIARAGVAEIIRDGLKIVIAGEPNAGKSSLLNALAQR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHC
DIAIVTEIAGTTRDVLSVDLSLAGFSVKLFDTAGLRETDEVVEREGIRRARQVIADADLV
CHHEEHHHCCCHHHHHHEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEE
LLLSEKPSDFRLDEWQPGKSVPVIRVATKVDRPMPRWKTSEADVFLSTRTGEGMDKLLAM
EEEECCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHH
LQAHLPDLAGNTALSMPSRRRHVDCLRQAGAALVRSMEARELELQAEQLRQAGDALGRIT
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRVDVEKLLDVIFSEFCIGK
CCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQFTDTIYALSSGSPPAGVALIRVSGPATADALARLCGPLPPARVATLRTIRTRNSDILD
CCCCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHC
SGLVLYFPGPASFTGEDCCELQVHGGRAVVSAILDELAAMDGLRHAEAGEFARRAFQNGK
CCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
LDLVEVEGLADLIAAETEMQRRLAVEQSGGGQSALYAGWARRLTHARAMIEAELDFADED
EEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DIPGSVSAAIWTDIGRLREEIDEHIARAGVAEIIRDGLKIVIAGEPNAGKSSLLNALAQR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHC
DIAIVTEIAGTTRDVLSVDLSLAGFSVKLFDTAGLRETDEVVEREGIRRARQVIADADLV
CHHEEHHHCCCHHHHHHEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEE
LLLSEKPSDFRLDEWQPGKSVPVIRVATKVDRPMPRWKTSEADVFLSTRTGEGMDKLLAM
EEEECCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHH
LQAHLPDLAGNTALSMPSRRRHVDCLRQAGAALVRSMEARELELQAEQLRQAGDALGRIT
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRVDVEKLLDVIFSEFCIGK
CCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA