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The map label for this gene is mnmG

Identifier: 150398396

GI number: 150398396

Start: 3377873

End: 3379744

Strand: Reverse

Name: mnmG

Synonym: Smed_3204

Alternate gene names: 150398396

Gene position: 3379744-3377873 (Counterclockwise)

Preceding gene: 150398397

Following gene: 150398395

Centisome position: 89.37

GC content: 58.6

Gene sequence:

>1872_bases
ATGGCGGATTATGATGTCATCGTAATTGGCGGAGGCCATGCGGGTTGTGAGGCCGCAGCGGCGTCGGCTCGGCTCGGCGC
GCGCACGCTGCTGATCACGCATAAGAAAGATACGATCGGGGTCATGTCCTGCAACCCCGCCATCGGCGGATTAGGCAAGG
GACACCTTGTCCGCGAGATTGATGCTCTCGATGGCCTTATGGGGCGCGTTGCTGACGCAGCCGGCATCCAGTTTAGGCTG
CTCAATCGACGCAAGGGACCTGCGGTCCGGGGGCCACGCACGCAGGCTGACAGAAAACTATATCGTGAGGCGATGCAGCG
TGAGATTGCTTCGATCGAGAATCTCGACGTCGCTGAGGGCGATGCGTTTGACCTGCAGACGGAGGACGGGGTTGTTTGTG
GGGCAGTGATGAAGGACGGACGAAGCTTCCGGGCAGCATCCGTAGTCCTCACAACAGGTACATTCCTCAGAGGGCTTATC
CATATAGGTGATCGGAAAATGCCGGCCGGTCGCGTCGGTGAGCAGCCGTCCGTCGGCCTTTCAGAAACGCTGGCACGTTT
CGGCCTGCAGCTTGGCCGCTTGAAGACCGGCACGCCGGCACGCCTGGATGGACGTACCATCGATTGGGGCCGTGTCGGGC
GTCAGGGTCCCGACGAAAATCCGGTGCCCTTCTCATTCATGACCGACGCGATCGTCAACCGTCAGATTGACTGCGGGGTG
ACCCGGACGACGGATGCGACGCACAAGATTATCGCCGACAACATTCACCGTTCGGCGATGTACTCGGGTCAAATTGAAGG
TGTGGGCCCCCGATACTGTCCTTCGATTGAGGACAAGATTGTTCGTTTCGGTGAAAGGGACGGGCACCAGATTTTCCTGG
AGCCGGAGGGGTTGGACGATGACACCGTCTATCCGAACGGGATTTCGACTTCTCTCCCGGAGGATGTGCAGGATGCCTTC
ATTCGGACCATCCCAGGACTTGAACAGGTCACGATACTTCAGCCGGGCTATGCCATCGAGTATGATCATGTAGACCCTAG
GGAACTTGAACCGTCTCTCGGCGTGCGTAGGATGCCGGGGCTGTTTCTTGCAGGACAGATCAATGGCACGACCGGCTATG
AGGAAGCTGGCGCGCAAGGTCTCGTCGCCGGTCTGAATGCCGCGCTCCGTGCAGCTGAACGCGCTCCGTTCTTCTTCAGC
CGAACCGACTCCTATATCGGGGTGATGATCGATGACCTAACTTCAAGAGGAATAGCTGAGCCGTATCGCATGTTCACGTC
CCGTGCTGAATACCGGCTGTCCCTGCGGGCGGACAACGCCGACATGCGACTTACGCCTGTTGGTATCGAACTCGGATGCG
TTGGCAATGCTCGCCTAACCCGCTTTCAACAGTGGAAGTCTGCCTATGAGGCCGGTCGCACGCTCCTGCAATCGCTTTCA
GTGACACCGAATGAAGGCAAGCGTTTCGGCTTGAAGCTTAATCAGGACGGGCAGCGCCGCACGGCATTCGAGATTCTCTC
CTATCCGGATCAATCGATCGAGGGTCTGAGGCCGCTTTGGCCGGAGCTTCAGGCCATTGCGACTGATGTTGCCGCCGCGT
TGGAGATTGATGCGGCCTATGCTGTCTATATGGAACGGCAGGCCGCCGACATCATCGGAGTACAGAGAGAAGAGAGTACA
GCCATTCCCGAGGCTTTTGATTACGAATCGCTGCCTGGGCTTTCGAATGAGCTGAAGCAGAAGCTCGCGCAGCAGAAGCC
GCGCAACCTTTCCCAGGCGATGAAGGTCGACGGCGTGACACCCGCGGCCATTTCACTGATCCTGTCATGGCTGCGACGAG
AAGACCGCCGTTCGAAGCGTGTAGGGGGTTGA

Upstream 100 bases:

>100_bases
CGAATACAACGCAGGCGAGTCATTGAGCCGTTTCACGTGAAACGTCCAGTCGAAGATATGGAACTTTTGATCAAAATGTG
CGTAAATTCGGAGCGATACT

Downstream 100 bases:

>100_bases
GTTCTGCAATGCAAACGAGTCTCAATCGAGCGCTGAACGGATTGCGTGTTTCACGTGAAACCGTCGAGAAACTGGAACAC
TTCGCTTCGCTCTTCCAGAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 623; Mature: 622

Protein sequence:

>623_residues
MADYDVIVIGGGHAGCEAAAASARLGARTLLITHKKDTIGVMSCNPAIGGLGKGHLVREIDALDGLMGRVADAAGIQFRL
LNRRKGPAVRGPRTQADRKLYREAMQREIASIENLDVAEGDAFDLQTEDGVVCGAVMKDGRSFRAASVVLTTGTFLRGLI
HIGDRKMPAGRVGEQPSVGLSETLARFGLQLGRLKTGTPARLDGRTIDWGRVGRQGPDENPVPFSFMTDAIVNRQIDCGV
TRTTDATHKIIADNIHRSAMYSGQIEGVGPRYCPSIEDKIVRFGERDGHQIFLEPEGLDDDTVYPNGISTSLPEDVQDAF
IRTIPGLEQVTILQPGYAIEYDHVDPRELEPSLGVRRMPGLFLAGQINGTTGYEEAGAQGLVAGLNAALRAAERAPFFFS
RTDSYIGVMIDDLTSRGIAEPYRMFTSRAEYRLSLRADNADMRLTPVGIELGCVGNARLTRFQQWKSAYEAGRTLLQSLS
VTPNEGKRFGLKLNQDGQRRTAFEILSYPDQSIEGLRPLWPELQAIATDVAAALEIDAAYAVYMERQAADIIGVQREEST
AIPEAFDYESLPGLSNELKQKLAQQKPRNLSQAMKVDGVTPAAISLILSWLRREDRRSKRVGG

Sequences:

>Translated_623_residues
MADYDVIVIGGGHAGCEAAAASARLGARTLLITHKKDTIGVMSCNPAIGGLGKGHLVREIDALDGLMGRVADAAGIQFRL
LNRRKGPAVRGPRTQADRKLYREAMQREIASIENLDVAEGDAFDLQTEDGVVCGAVMKDGRSFRAASVVLTTGTFLRGLI
HIGDRKMPAGRVGEQPSVGLSETLARFGLQLGRLKTGTPARLDGRTIDWGRVGRQGPDENPVPFSFMTDAIVNRQIDCGV
TRTTDATHKIIADNIHRSAMYSGQIEGVGPRYCPSIEDKIVRFGERDGHQIFLEPEGLDDDTVYPNGISTSLPEDVQDAF
IRTIPGLEQVTILQPGYAIEYDHVDPRELEPSLGVRRMPGLFLAGQINGTTGYEEAGAQGLVAGLNAALRAAERAPFFFS
RTDSYIGVMIDDLTSRGIAEPYRMFTSRAEYRLSLRADNADMRLTPVGIELGCVGNARLTRFQQWKSAYEAGRTLLQSLS
VTPNEGKRFGLKLNQDGQRRTAFEILSYPDQSIEGLRPLWPELQAIATDVAAALEIDAAYAVYMERQAADIIGVQREEST
AIPEAFDYESLPGLSNELKQKLAQQKPRNLSQAMKVDGVTPAAISLILSWLRREDRRSKRVGG
>Mature_622_residues
ADYDVIVIGGGHAGCEAAAASARLGARTLLITHKKDTIGVMSCNPAIGGLGKGHLVREIDALDGLMGRVADAAGIQFRLL
NRRKGPAVRGPRTQADRKLYREAMQREIASIENLDVAEGDAFDLQTEDGVVCGAVMKDGRSFRAASVVLTTGTFLRGLIH
IGDRKMPAGRVGEQPSVGLSETLARFGLQLGRLKTGTPARLDGRTIDWGRVGRQGPDENPVPFSFMTDAIVNRQIDCGVT
RTTDATHKIIADNIHRSAMYSGQIEGVGPRYCPSIEDKIVRFGERDGHQIFLEPEGLDDDTVYPNGISTSLPEDVQDAFI
RTIPGLEQVTILQPGYAIEYDHVDPRELEPSLGVRRMPGLFLAGQINGTTGYEEAGAQGLVAGLNAALRAAERAPFFFSR
TDSYIGVMIDDLTSRGIAEPYRMFTSRAEYRLSLRADNADMRLTPVGIELGCVGNARLTRFQQWKSAYEAGRTLLQSLSV
TPNEGKRFGLKLNQDGQRRTAFEILSYPDQSIEGLRPLWPELQAIATDVAAALEIDAAYAVYMERQAADIIGVQREESTA
IPEAFDYESLPGLSNELKQKLAQQKPRNLSQAMKVDGVTPAAISLILSWLRREDRRSKRVGG

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=634, Percent_Identity=46.3722397476341, Blast_Score=552, Evalue=1e-157,
Organism=Homo sapiens, GI19882217, Length=659, Percent_Identity=44.6130500758725, Blast_Score=538, Evalue=1e-153,
Organism=Homo sapiens, GI183227703, Length=674, Percent_Identity=43.620178041543, Blast_Score=533, Evalue=1e-151,
Organism=Escherichia coli, GI2367273, Length=617, Percent_Identity=52.1880064829822, Blast_Score=605, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI17534255, Length=618, Percent_Identity=45.1456310679612, Blast_Score=529, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6321202, Length=618, Percent_Identity=48.0582524271845, Blast_Score=574, Evalue=1e-164,
Organism=Drosophila melanogaster, GI24658174, Length=630, Percent_Identity=47.4603174603175, Blast_Score=542, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_SINMW (A6UEE8)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001328863.1
- ProteinModelPortal:   A6UEE8
- SMR:   A6UEE8
- STRING:   A6UEE8
- GeneID:   5324083
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_3204
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   SMED366394:SMED_3204-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 67955; Mature: 67824

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADYDVIVIGGGHAGCEAAAASARLGARTLLITHKKDTIGVMSCNPAIGGLGKGHLVREI
CCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHH
DALDGLMGRVADAAGIQFRLLNRRKGPAVRGPRTQADRKLYREAMQREIASIENLDVAEG
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
DAFDLQTEDGVVCGAVMKDGRSFRAASVVLTTGTFLRGLIHIGDRKMPAGRVGEQPSVGL
CCEEEECCCCEEEEHHHHCCCCEEEEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SETLARFGLQLGRLKTGTPARLDGRTIDWGRVGRQGPDENPVPFSFMTDAIVNRQIDCGV
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
TRTTDATHKIIADNIHRSAMYSGQIEGVGPRYCPSIEDKIVRFGERDGHQIFLEPEGLDD
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCC
DTVYPNGISTSLPEDVQDAFIRTIPGLEQVTILQPGYAIEYDHVDPRELEPSLGVRRMPG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCHHHCCCCCCCCCCCC
LFLAGQINGTTGYEEAGAQGLVAGLNAALRAAERAPFFFSRTDSYIGVMIDDLTSRGIAE
EEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEHHHHCCCCHH
PYRMFTSRAEYRLSLRADNADMRLTPVGIELGCVGNARLTRFQQWKSAYEAGRTLLQSLS
HHHHHHCCCCEEEEEEECCCCEEEEEECEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
VTPNEGKRFGLKLNQDGQRRTAFEILSYPDQSIEGLRPLWPELQAIATDVAAALEIDAAY
CCCCCCCEEEEEECCCCCHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVYMERQAADIIGVQREESTAIPEAFDYESLPGLSNELKQKLAQQKPRNLSQAMKVDGVT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCCC
PAAISLILSWLRREDRRSKRVGG
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADYDVIVIGGGHAGCEAAAASARLGARTLLITHKKDTIGVMSCNPAIGGLGKGHLVREI
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHH
DALDGLMGRVADAAGIQFRLLNRRKGPAVRGPRTQADRKLYREAMQREIASIENLDVAEG
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
DAFDLQTEDGVVCGAVMKDGRSFRAASVVLTTGTFLRGLIHIGDRKMPAGRVGEQPSVGL
CCEEEECCCCEEEEHHHHCCCCEEEEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SETLARFGLQLGRLKTGTPARLDGRTIDWGRVGRQGPDENPVPFSFMTDAIVNRQIDCGV
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
TRTTDATHKIIADNIHRSAMYSGQIEGVGPRYCPSIEDKIVRFGERDGHQIFLEPEGLDD
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCC
DTVYPNGISTSLPEDVQDAFIRTIPGLEQVTILQPGYAIEYDHVDPRELEPSLGVRRMPG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCHHHCCCCCCCCCCCC
LFLAGQINGTTGYEEAGAQGLVAGLNAALRAAERAPFFFSRTDSYIGVMIDDLTSRGIAE
EEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEHHHHCCCCHH
PYRMFTSRAEYRLSLRADNADMRLTPVGIELGCVGNARLTRFQQWKSAYEAGRTLLQSLS
HHHHHHCCCCEEEEEEECCCCEEEEEECEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
VTPNEGKRFGLKLNQDGQRRTAFEILSYPDQSIEGLRPLWPELQAIATDVAAALEIDAAY
CCCCCCCEEEEEECCCCCHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVYMERQAADIIGVQREESTAIPEAFDYESLPGLSNELKQKLAQQKPRNLSQAMKVDGVT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCCC
PAAISLILSWLRREDRRSKRVGG
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA