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The map label for this gene is hisJ [H]

Identifier: 150398296

GI number: 150398296

Start: 3247507

End: 3248316

Strand: Direct

Name: hisJ [H]

Synonym: Smed_3102

Alternate gene names: 150398296

Gene position: 3247507-3248316 (Clockwise)

Preceding gene: 150398295

Following gene: 150398297

Centisome position: 85.87

GC content: 60.74

Gene sequence:

>810_bases
ATGACAATACGTCGCCACGTGCTTGCGGGCATTGCCGCAGTTCTTGCCGCTCCACTCGCAGTCTCCGCGCCCGCTTTCGC
AAGCGACCTGCCCGACCTCGGCGGCAAGACGGTCGTCGTGGTGACCGAGAACGCCTACCCCCCGCTGCAGTTCGTGGATC
CCAAATCCGGCCAGGCCATCGGCTGGGAATATGACGCGATGAACGAGATCGCCAAGCGGCTGAACTTCAAGGTCGAATAT
CAGAACACCAGCTGGGATGCGATGATCCAGGCCGTTTCCGACGGTCAGTACCAGATCGGAATGACCGGGATCACCATCAA
GGACGACCGCAAGGAAAAGGTCGATTTTTCCGATCCCTACCTGCGATCGGAGCAGTTCATGCTGGTGCGGGGCGACGAGA
CCCGCTTTAGCGATGCGAAGAGTTTCGCGGCGCTTGAAAGCGGGTTGATCGGCGCTCAGCCCGGTACCTCGCCGTTCTAC
ACGGCAGTTTACGAAATTCTCGATGGCAACGAGCAGAACCCGCGGATCAAGCTGTTCGAGACCTTCGGTGCGACGGTGCA
GGCCCTGAAGGCCGGCGACGTCGATCTGGTCCTGACCGACAGCGTTGCGGCCAAGGGCTATGTCGATTCCTCCGAAGGCC
AGCTCAAGGTGGTCGGCGGGCCGCTCGGCACCGAGGATTTCGGCTTCATCTTCCCGAAGGGATCGGAGCTTGTGGCGCCG
GTCAATGCCGCCATCAAGGCGCTCAAGGACGACGGCACCTTGGACGCGCTCAACAAGAAGTGGTTTCTCGATTACAAAAT
GGGCGGCTGA

Upstream 100 bases:

>100_bases
GAAAGGCGGCCTCAGGGCCGCCTTTCTTTTTGCACCTGCCGCTTGTGTTCCCCATAGATTTTATGACATTTTCGGCCGCC
ACAATCTTTGGGAGTCCTCA

Downstream 100 bases:

>100_bases
TCCCTGGAGCGGGAGAGGAATCGTGTGCGGCTTGCTGCCGCACCCCGCTCCCCGTTATTGGAATTGTAATGACGCCGGTC
CAATCATCCGACACTCCGTC

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MTIRRHVLAGIAAVLAAPLAVSAPAFASDLPDLGGKTVVVVTENAYPPLQFVDPKSGQAIGWEYDAMNEIAKRLNFKVEY
QNTSWDAMIQAVSDGQYQIGMTGITIKDDRKEKVDFSDPYLRSEQFMLVRGDETRFSDAKSFAALESGLIGAQPGTSPFY
TAVYEILDGNEQNPRIKLFETFGATVQALKAGDVDLVLTDSVAAKGYVDSSEGQLKVVGGPLGTEDFGFIFPKGSELVAP
VNAAIKALKDDGTLDALNKKWFLDYKMGG

Sequences:

>Translated_269_residues
MTIRRHVLAGIAAVLAAPLAVSAPAFASDLPDLGGKTVVVVTENAYPPLQFVDPKSGQAIGWEYDAMNEIAKRLNFKVEY
QNTSWDAMIQAVSDGQYQIGMTGITIKDDRKEKVDFSDPYLRSEQFMLVRGDETRFSDAKSFAALESGLIGAQPGTSPFY
TAVYEILDGNEQNPRIKLFETFGATVQALKAGDVDLVLTDSVAAKGYVDSSEGQLKVVGGPLGTEDFGFIFPKGSELVAP
VNAAIKALKDDGTLDALNKKWFLDYKMGG
>Mature_268_residues
TIRRHVLAGIAAVLAAPLAVSAPAFASDLPDLGGKTVVVVTENAYPPLQFVDPKSGQAIGWEYDAMNEIAKRLNFKVEYQ
NTSWDAMIQAVSDGQYQIGMTGITIKDDRKEKVDFSDPYLRSEQFMLVRGDETRFSDAKSFAALESGLIGAQPGTSPFYT
AVYEILDGNEQNPRIKLFETFGATVQALKAGDVDLVLTDSVAAKGYVDSSEGQLKVVGGPLGTEDFGFIFPKGSELVAPV
NAAIKALKDDGTLDALNKKWFLDYKMGG

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=254, Percent_Identity=29.5275590551181, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788228, Length=262, Percent_Identity=30.9160305343511, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787088, Length=260, Percent_Identity=23.8461538461538, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787085, Length=225, Percent_Identity=24.8888888888889, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788649, Length=271, Percent_Identity=25.830258302583, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29046; Mature: 28915

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRRHVLAGIAAVLAAPLAVSAPAFASDLPDLGGKTVVVVTENAYPPLQFVDPKSGQAI
CCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEECCCCCCCEEECCCCCCEE
GWEYDAMNEIAKRLNFKVEYQNTSWDAMIQAVSDGQYQIGMTGITIKDDRKEKVDFSDPY
CCCHHHHHHHHHHCCEEEEECCCCHHHHHEECCCCCEEEEEEEEEEECCCCCCCCCCCCC
LRSEQFMLVRGDETRFSDAKSFAALESGLIGAQPGTSPFYTAVYEILDGNEQNPRIKLFE
CCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEE
TFGATVQALKAGDVDLVLTDSVAAKGYVDSSEGQLKVVGGPLGTEDFGFIFPKGSELVAP
HHCCCEEHHHCCCEEEEEECCCCCCCCEECCCCEEEEEECCCCCCCCEEEECCCCCEEEH
VNAAIKALKDDGTLDALNKKWFLDYKMGG
HHHHHHHHCCCCCEECCCCEEEEEEECCC
>Mature Secondary Structure 
TIRRHVLAGIAAVLAAPLAVSAPAFASDLPDLGGKTVVVVTENAYPPLQFVDPKSGQAI
CHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEECCCCCCCEEECCCCCCEE
GWEYDAMNEIAKRLNFKVEYQNTSWDAMIQAVSDGQYQIGMTGITIKDDRKEKVDFSDPY
CCCHHHHHHHHHHCCEEEEECCCCHHHHHEECCCCCEEEEEEEEEEECCCCCCCCCCCCC
LRSEQFMLVRGDETRFSDAKSFAALESGLIGAQPGTSPFYTAVYEILDGNEQNPRIKLFE
CCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEE
TFGATVQALKAGDVDLVLTDSVAAKGYVDSSEGQLKVVGGPLGTEDFGFIFPKGSELVAP
HHCCCEEHHHCCCEEEEEECCCCCCCCEECCCCEEEEEECCCCCCCCEEEECCCCCEEEH
VNAAIKALKDDGTLDALNKKWFLDYKMGG
HHHHHHHHCCCCCEECCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]