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The map label for this gene is yxeN [H]

Identifier: 150398297

GI number: 150398297

Start: 3248385

End: 3249209

Strand: Direct

Name: yxeN [H]

Synonym: Smed_3103

Alternate gene names: 150398297

Gene position: 3248385-3249209 (Clockwise)

Preceding gene: 150398296

Following gene: 150398301

Centisome position: 85.89

GC content: 61.7

Gene sequence:

>825_bases
ATGACGCCGGTCCAATCATCCGACACTCCGTCTCAGAAGGGCGATTATCCCTGGTGGCTGATCGCCCTGATCCTCATCGC
GGCGGCGCTGGCTCTTGTCATTGCTGCCAATGACATATTCACGCAGGTATTCACAGTCGTCCTGAAGGGACTGGGCGTCA
CCGTATTCGTAACTCTCGTCGGCTTCGTGCTTGCGACGCTGCTCGGCCTCGGTGTCGCCCTGATGGCGCTTTCCGGGCAT
GTTGCGCTGCGCCAGATTGCGCGCTTCTACACGGAAGTCATCCGCGGCGTGCCTATTCTCGTGCTCCTGTTCTACATCGC
CTTCGTCGGCGCGCCAGCGTTGGTAACGGCTGCGAATTTCGTGGCGGCCCCCCTGATCTCGGCGGGATGGATGGAGGCGT
TCGTCGTGCGCGACGTTTCCCTGATGTGGCGCGCGATCATCGCGCTGATGATCGGCTACTCGGCCTTCATCGCCGAGGTA
TTCCGCGCCGGCATCCAGTCGGTCGACAAGGGCCAGGTGGAGGCGGCGAAGGCGCTCGGGCTTTCCCGCTACCAGCGTTT
TCGCCTCGTCGTCTTTCCGCAGGCAATCCGCGTCATCCTGCCGCCGCTGGGCAACGATTTCGTGGCCATGGTCAAGGATT
CCTCGCTCGTCTCGGTGCTCGGCGTCGCCGACGTGACCCAGATGGGCAAGATCTATGCCTCGGGATCCTTCCGCTTTTTC
GAGACCTACTCCATCGTTGCCTATGTCTATCTCGTGCTGACGATAGGGCTTTCACTGGCGCTAAGGGCTGTCGAGCAGCG
TCTGAGGCGGTCGCAAGTCCGCTAA

Upstream 100 bases:

>100_bases
AGTGGTTTCTCGATTACAAAATGGGCGGCTGATCCCTGGAGCGGGAGAGGAATCGTGTGCGGCTTGCTGCCGCACCCCGC
TCCCCGTTATTGGAATTGTA

Downstream 100 bases:

>100_bases
AGCTTCCAAGAAAAGTGCGTAGCGGAGAAAAGCGGAAATTCTCTAGACCACTGCATGTTTCCTTTGATCAGATCCGATTA
GAGGAGAAAAACATCCAGCA

Product: polar amino acid ABC transporter inner membrane subunit

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTPVQSSDTPSQKGDYPWWLIALILIAAALALVIAANDIFTQVFTVVLKGLGVTVFVTLVGFVLATLLGLGVALMALSGH
VALRQIARFYTEVIRGVPILVLLFYIAFVGAPALVTAANFVAAPLISAGWMEAFVVRDVSLMWRAIIALMIGYSAFIAEV
FRAGIQSVDKGQVEAAKALGLSRYQRFRLVVFPQAIRVILPPLGNDFVAMVKDSSLVSVLGVADVTQMGKIYASGSFRFF
ETYSIVAYVYLVLTIGLSLALRAVEQRLRRSQVR

Sequences:

>Translated_274_residues
MTPVQSSDTPSQKGDYPWWLIALILIAAALALVIAANDIFTQVFTVVLKGLGVTVFVTLVGFVLATLLGLGVALMALSGH
VALRQIARFYTEVIRGVPILVLLFYIAFVGAPALVTAANFVAAPLISAGWMEAFVVRDVSLMWRAIIALMIGYSAFIAEV
FRAGIQSVDKGQVEAAKALGLSRYQRFRLVVFPQAIRVILPPLGNDFVAMVKDSSLVSVLGVADVTQMGKIYASGSFRFF
ETYSIVAYVYLVLTIGLSLALRAVEQRLRRSQVR
>Mature_273_residues
TPVQSSDTPSQKGDYPWWLIALILIAAALALVIAANDIFTQVFTVVLKGLGVTVFVTLVGFVLATLLGLGVALMALSGHV
ALRQIARFYTEVIRGVPILVLLFYIAFVGAPALVTAANFVAAPLISAGWMEAFVVRDVSLMWRAIIALMIGYSAFIAEVF
RAGIQSVDKGQVEAAKALGLSRYQRFRLVVFPQAIRVILPPLGNDFVAMVKDSSLVSVLGVADVTQMGKIYASGSFRFFE
TYSIVAYVYLVLTIGLSLALRAVEQRLRRSQVR

Specific function: Probably part of the ABC transporter complex yxeMNO that could be involved in amino-acid import. May transport S- methylcysteine. Probably responsible for the translocation of the substrate across the membrane (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787030, Length=224, Percent_Identity=34.8214285714286, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788226, Length=226, Percent_Identity=32.3008849557522, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788646, Length=222, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786874, Length=224, Percent_Identity=26.7857142857143, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786873, Length=225, Percent_Identity=28, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI48994930, Length=123, Percent_Identity=39.8373983739837, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787087, Length=189, Percent_Identity=31.7460317460317, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082239, Length=223, Percent_Identity=29.1479820627803, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788645, Length=219, Percent_Identity=24.2009132420091, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29706; Mature: 29575

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPVQSSDTPSQKGDYPWWLIALILIAAALALVIAANDIFTQVFTVVLKGLGVTVFVTLV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFVLATLLGLGVALMALSGHVALRQIARFYTEVIRGVPILVLLFYIAFVGAPALVTAANF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHH
VAAPLISAGWMEAFVVRDVSLMWRAIIALMIGYSAFIAEVFRAGIQSVDKGQVEAAKALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LSRYQRFRLVVFPQAIRVILPPLGNDFVAMVKDSSLVSVLGVADVTQMGKIYASGSFRFF
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
ETYSIVAYVYLVLTIGLSLALRAVEQRLRRSQVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]