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The map label for this gene is trmD [H]

Identifier: 150398293

GI number: 150398293

Start: 3245086

End: 3245808

Strand: Direct

Name: trmD [H]

Synonym: Smed_3099

Alternate gene names: 150398293

Gene position: 3245086-3245808 (Clockwise)

Preceding gene: 150398292

Following gene: 150398295

Centisome position: 85.81

GC content: 65.56

Gene sequence:

>723_bases
ATGCTCTTTCGTGCAACGGTCCTGACGCTCTATCCCGAAATGTTCCCCGGACATCTCGGCGTTTCGCTCGCCGGAAAGGC
ACTGGAGCGCGGGGATTGGTCGATCGAAGCCGTGCAGATACGCGACTTCGCCGGCGACAGGCACCGCACGGTAGACGACA
CGCCGGCCGGCGGAGGCGCCGGAATGGTGCTGAAGCCCGACGTTCTTGCCCGCGCTATCGACCATGTTTCGGCAGATGAC
GAGCGACCGCGGCTTCTGATGAGCCCGCGCGGGCGGCCGCTGACGCAGGAACGGGTGCGCGCGCTGGCCGCCGGTCCCGG
CGTCGTCGTCGTTTGCGGCCGTTTCGAGGGTGTCGATCAGCGGGTCATCGATACGCGCCAGCTGGAGGAAGTCTCGATCG
GCGACTACATTCTTTCCGGCGGCGAACCGGCGGCACTGGTCCTGCTGGACGCGGTGGTGCGCATTCTGCCGGGCGTCATG
GGAAACGAACTCTCGGGCGTGCACGAGAGCTTCGAAGGAGGGTTGCTGGAGCATCCCCACTACACGCGTCCCCAGGTCTT
CGAGGGTCAAGAAATACCAGCCGTGCTCACGTCAGGCAATCACGCCGCTATAGCCCAATGGCGATTGGTCGCCGCCCGCC
GGCTGACCGCCGAGCGCCGGCCGGACCTCCTGGAGCGTGATCTGAAACCGCAGCCTGATCCGGCTCAACCCGTGAAGAAG
TAA

Upstream 100 bases:

>100_bases
GGCAAACTCGTCATCGACCCGACTGCAGCAGGGCTGGTCGATGATGAGAAGAGTGGCCCAGGCAAGCCGTTTCCGACCAA
GCGCAAGTGAGCCCCCGGCG

Downstream 100 bases:

>100_bases
TAGGCGGCCAGCAGGAGCCCCACTGCCACGACCACGGCGCGTATCACCGACTGCGGAACGCGGCGTGCCGCCCATACGCC
CATATAGCCGCCGAGGGCTG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MLFRATVLTLYPEMFPGHLGVSLAGKALERGDWSIEAVQIRDFAGDRHRTVDDTPAGGGAGMVLKPDVLARAIDHVSADD
ERPRLLMSPRGRPLTQERVRALAAGPGVVVVCGRFEGVDQRVIDTRQLEEVSIGDYILSGGEPAALVLLDAVVRILPGVM
GNELSGVHESFEGGLLEHPHYTRPQVFEGQEIPAVLTSGNHAAIAQWRLVAARRLTAERRPDLLERDLKPQPDPAQPVKK

Sequences:

>Translated_240_residues
MLFRATVLTLYPEMFPGHLGVSLAGKALERGDWSIEAVQIRDFAGDRHRTVDDTPAGGGAGMVLKPDVLARAIDHVSADD
ERPRLLMSPRGRPLTQERVRALAAGPGVVVVCGRFEGVDQRVIDTRQLEEVSIGDYILSGGEPAALVLLDAVVRILPGVM
GNELSGVHESFEGGLLEHPHYTRPQVFEGQEIPAVLTSGNHAAIAQWRLVAARRLTAERRPDLLERDLKPQPDPAQPVKK
>Mature_240_residues
MLFRATVLTLYPEMFPGHLGVSLAGKALERGDWSIEAVQIRDFAGDRHRTVDDTPAGGGAGMVLKPDVLARAIDHVSADD
ERPRLLMSPRGRPLTQERVRALAAGPGVVVVCGRFEGVDQRVIDTRQLEEVSIGDYILSGGEPAALVLLDAVVRILPGVM
GNELSGVHESFEGGLLEHPHYTRPQVFEGQEIPAVLTSGNHAAIAQWRLVAARRLTAERRPDLLERDLKPQPDPAQPVKK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=222, Percent_Identity=48.1981981981982, Blast_Score=199, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 26103; Mature: 26103

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFRATVLTLYPEMFPGHLGVSLAGKALERGDWSIEAVQIRDFAGDRHRTVDDTPAGGGA
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
GMVLKPDVLARAIDHVSADDERPRLLMSPRGRPLTQERVRALAAGPGVVVVCGRFEGVDQ
CEEECHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCH
RVIDTRQLEEVSIGDYILSGGEPAALVLLDAVVRILPGVMGNELSGVHESFEGGLLEHPH
HHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCC
YTRPQVFEGQEIPAVLTSGNHAAIAQWRLVAARRLTAERRPDLLERDLKPQPDPAQPVKK
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLFRATVLTLYPEMFPGHLGVSLAGKALERGDWSIEAVQIRDFAGDRHRTVDDTPAGGGA
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
GMVLKPDVLARAIDHVSADDERPRLLMSPRGRPLTQERVRALAAGPGVVVVCGRFEGVDQ
CEEECHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCH
RVIDTRQLEEVSIGDYILSGGEPAALVLLDAVVRILPGVMGNELSGVHESFEGGLLEHPH
HHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCC
YTRPQVFEGQEIPAVLTSGNHAAIAQWRLVAARRLTAERRPDLLERDLKPQPDPAQPVKK
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]