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The map label for this gene is rlmN

Identifier: 150398262

GI number: 150398262

Start: 3216694

End: 3217935

Strand: Reverse

Name: rlmN

Synonym: Smed_3068

Alternate gene names: 150398262

Gene position: 3217935-3216694 (Counterclockwise)

Preceding gene: 150398263

Following gene: 150398261

Centisome position: 85.09

GC content: 61.67

Gene sequence:

>1242_bases
ATGAGCATGGCAGCCACCGAAACCTTGAACAGGACCGACATCGCCAAGGCGCCGCAGGTGCGGCTTGCGGCACAGCCGGA
AAAGCCGTCGCTGATCGGCCTGTTGCGCGAAGATATGGCGAAGCTGCTCGTCGAGAAAGGCGTGCCGGAGCGCCAGGTGA
AGATGCGCGTCAGCCAAGTCTGGCACTGGCTTTATGTCCGCGGCGTCTCGGATTTCAACGAGATGTCGAATGTCTCCAAG
GACATGCGCGAGATGCTCAGTGCGCATTTCACCATCGCGCGGCCGGAGATCGTCGAAGAGCAGGTTTCCGGCGACGGCAC
GCGCAAATGGCTGCTGCGCTTCCCTCCGCGCGGAGCCGGCCGCCCGGTGGAGATCGAGACGGTCTACATTCCCGAGGAAG
GCCGCGGTACGCTCTGCATTTCCAGCCAAGTCGGCTGCACGCTCACCTGCTCCTTCTGTCATACCGGCACGCAGAAGCTG
GTGCGCAATCTGACGGCGGAAGAAATTCTCGCGCAGCTTCTGCTTGCGCGTGACCGGCTCGGCGATTTCCCGGATCGCGA
CACGCCGCAGGGCGCCATCGTACCGGCGGAAGGCCGCAAGATCACCAATGTCGTGATGATGGGCATGGGCGAGCCGCTCT
ACAATTTCGAGAACGTCAAGACGGCGCTTCTGATCGCCTCCGACGGCGACGGGCTGTCGCTTTCGAAGCGACGCATCACC
CTTTCGACCTCGGGCATTGTGCCGGAGATCTACCGCACCGGCGAGGAAATCGGGGTCATGCTCGCGATCTCGCTTCACGC
CGTGCGCGACGATCTGCGCGATATGCTGGTGCCGATCAACAAGAAGTATCCGCTGAAGGAGCTGATGGAGGCCTGCCGCG
CCTATCCGGGCTTGTCGAATGCCCGCCGCATCACCTTCGAATATGTGATGCTGAAGGATGTCAACGACAGCCTGGAAGAC
GCCAAGGAACTGGTGAAGCTCCTGAAGGGCATTCCCGCCAAGATAAACCTGATCCCGTTCAATCCCTGGCCGGGAACCAA
TTACCAGTGCTCGGACTGGGAGCAGATCGAGAAGTTTGCCGATTTCATCAATCAGGCGGGCTACGCTTCGCCGATCCGCA
CCCCGCGCGGGCGCGACATTCTGGCCGCTTGCGGCCAGCTTAAGTCGGACTCGGAGCGCATGCGCAAGGTCGACCGCCTT
GCCTTCGAGGCGATGATGATTGCCAATCACGGCGAGGACTAG

Upstream 100 bases:

>100_bases
CGTCGAGCGCGCGACCGAGGCTCGCCAGATTTCAATTCGAGCATCGGTACAGGGTCCGGAGACGTCTCCGGACGAGCCGC
TGCTTTACGACAGGACAAAT

Downstream 100 bases:

>100_bases
ACGTCCGATCGCCCGCCGAAGGCAACGGCTTCATGATCAAACGTCTTCACCGACCGCAGTATTCGTGCGGTCGGGTATTT
GCCGATTGATTTCGGCCCCC

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSMAATETLNRTDIAKAPQVRLAAQPEKPSLIGLLREDMAKLLVEKGVPERQVKMRVSQVWHWLYVRGVSDFNEMSNVSK
DMREMLSAHFTIARPEIVEEQVSGDGTRKWLLRFPPRGAGRPVEIETVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKL
VRNLTAEEILAQLLLARDRLGDFPDRDTPQGAIVPAEGRKITNVVMMGMGEPLYNFENVKTALLIASDGDGLSLSKRRIT
LSTSGIVPEIYRTGEEIGVMLAISLHAVRDDLRDMLVPINKKYPLKELMEACRAYPGLSNARRITFEYVMLKDVNDSLED
AKELVKLLKGIPAKINLIPFNPWPGTNYQCSDWEQIEKFADFINQAGYASPIRTPRGRDILAACGQLKSDSERMRKVDRL
AFEAMMIANHGED

Sequences:

>Translated_413_residues
MSMAATETLNRTDIAKAPQVRLAAQPEKPSLIGLLREDMAKLLVEKGVPERQVKMRVSQVWHWLYVRGVSDFNEMSNVSK
DMREMLSAHFTIARPEIVEEQVSGDGTRKWLLRFPPRGAGRPVEIETVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKL
VRNLTAEEILAQLLLARDRLGDFPDRDTPQGAIVPAEGRKITNVVMMGMGEPLYNFENVKTALLIASDGDGLSLSKRRIT
LSTSGIVPEIYRTGEEIGVMLAISLHAVRDDLRDMLVPINKKYPLKELMEACRAYPGLSNARRITFEYVMLKDVNDSLED
AKELVKLLKGIPAKINLIPFNPWPGTNYQCSDWEQIEKFADFINQAGYASPIRTPRGRDILAACGQLKSDSERMRKVDRL
AFEAMMIANHGED
>Mature_412_residues
SMAATETLNRTDIAKAPQVRLAAQPEKPSLIGLLREDMAKLLVEKGVPERQVKMRVSQVWHWLYVRGVSDFNEMSNVSKD
MREMLSAHFTIARPEIVEEQVSGDGTRKWLLRFPPRGAGRPVEIETVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLV
RNLTAEEILAQLLLARDRLGDFPDRDTPQGAIVPAEGRKITNVVMMGMGEPLYNFENVKTALLIASDGDGLSLSKRRITL
STSGIVPEIYRTGEEIGVMLAISLHAVRDDLRDMLVPINKKYPLKELMEACRAYPGLSNARRITFEYVMLKDVNDSLEDA
KELVKLLKGIPAKINLIPFNPWPGTNYQCSDWEQIEKFADFINQAGYASPIRTPRGRDILAACGQLKSDSERMRKVDRLA
FEAMMIANHGED

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=365, Percent_Identity=45.7534246575342, Blast_Score=295, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_SINMW (A6UE14)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001328729.1
- STRING:   A6UE14
- GeneID:   5323947
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_3068
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   CLSK864022
- BioCyc:   SMED366394:SMED_3068-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 46252; Mature: 46121

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMAATETLNRTDIAKAPQVRLAAQPEKPSLIGLLREDMAKLLVEKGVPERQVKMRVSQV
CCCCHHCCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
WHWLYVRGVSDFNEMSNVSKDMREMLSAHFTIARPEIVEEQVSGDGTRKWLLRFPPRGAG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHCCCCCEEEEEECCCCCCC
RPVEIETVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTAEEILAQLLLARDRL
CCEEEEEEEECCCCCCEEEEECCCCCEEEEHHHHCCHHHHHHHCCHHHHHHHHHHHHHHC
GDFPDRDTPQGAIVPAEGRKITNVVMMGMGEPLYNFENVKTALLIASDGDGLSLSKRRIT
CCCCCCCCCCCCEEECCCCEEEHHHEECCCCCCCCCCCCEEEEEEEECCCCCEEECEEEE
LSTSGIVPEIYRTGEEIGVMLAISLHAVRDDLRDMLVPINKKYPLKELMEACRAYPGLSN
EECCCCCHHHHHCCHHHCEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCC
ARRITFEYVMLKDVNDSLEDAKELVKLLKGIPAKINLIPFNPWPGTNYQCSDWEQIEKFA
CCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
DFINQAGYASPIRTPRGRDILAACGQLKSDSERMRKVDRLAFEAMMIANHGED
HHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SMAATETLNRTDIAKAPQVRLAAQPEKPSLIGLLREDMAKLLVEKGVPERQVKMRVSQV
CCCHHCCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
WHWLYVRGVSDFNEMSNVSKDMREMLSAHFTIARPEIVEEQVSGDGTRKWLLRFPPRGAG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHCCCCCEEEEEECCCCCCC
RPVEIETVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTAEEILAQLLLARDRL
CCEEEEEEEECCCCCCEEEEECCCCCEEEEHHHHCCHHHHHHHCCHHHHHHHHHHHHHHC
GDFPDRDTPQGAIVPAEGRKITNVVMMGMGEPLYNFENVKTALLIASDGDGLSLSKRRIT
CCCCCCCCCCCCEEECCCCEEEHHHEECCCCCCCCCCCCEEEEEEEECCCCCEEECEEEE
LSTSGIVPEIYRTGEEIGVMLAISLHAVRDDLRDMLVPINKKYPLKELMEACRAYPGLSN
EECCCCCHHHHHCCHHHCEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCC
ARRITFEYVMLKDVNDSLEDAKELVKLLKGIPAKINLIPFNPWPGTNYQCSDWEQIEKFA
CCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
DFINQAGYASPIRTPRGRDILAACGQLKSDSERMRKVDRLAFEAMMIANHGED
HHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA