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The map label for this gene is acoA [H]

Identifier: 150398233

GI number: 150398233

Start: 3187474

End: 3188502

Strand: Reverse

Name: acoA [H]

Synonym: Smed_3039

Alternate gene names: 150398233

Gene position: 3188502-3187474 (Counterclockwise)

Preceding gene: 150398234

Following gene: 150398232

Centisome position: 84.31

GC content: 62.29

Gene sequence:

>1029_bases
GTGGACGCTCCTGTGAATACCAACCTGCTCGAGCTCTATCGGACGATGCGGCGCATCCGCACCTTCGAGGAACGGGTCGG
CGAACTCTTCGTGCGCGGACAATCCGCAGGTTCGATGCTGCATCTTTCCATAGGCGAGGAGTCGAGCGCTGCGGGCGTCT
GCGCGGCAATGAAGCCCCAGGACACCTTCACGACCCACCATCGCGGCCATGGCATCTTCCTCGCACGCGGTGCCGATCCT
AAGCGGATGATGGCTGAAATCGGCGGCAAGGAAACCGGTTACTGCCGCGGAAAGGGCGGTTCGATGCACATCGCGGACAT
GGCGCTCGGTCATCTTGGTGCCAATGCCATTGTCGGCGGCGGCATACCCGCCGTGATCGGTGCCGGTCTGTCGAGCAGGC
ATCTCAAGCAGGATTCGGTCTCGATCGCCTTCTTTGGGGACGGGGCCATGCAGCAGGGCATCCTGTACGAGAGCATGAAC
ATGGCTTCGCTCTGGAACCTGCCGGTGCTCTTCGTCTGCATCAACAATCAGTACGGCATGGGCACGCGCATCGACCAGGC
GACCCGCAACACGGCATTCGACCAGCGGGCCAAGGCCTTTGGGCTGAACGGCGCGGTGGTCGACGGCCTCGACGTCGAGG
AAGTTCAAGCCGCGGCGCGCTGGCTCGTTGACGAGGCGCGCGCCGGCAAGCCCGGATTCCTGTCCGTCGAGGTCTATCGT
TTCTTCGGCCATGCACGCATGGACAAAAGTCCCTATCGCGAGGAGGCGGAGGAACTGGAAGGCCGCAAAAAGGATCCGGT
GCTTTTCGCGCGCAACAAGCTGATCGACACCGGTATCGAGGAGGAGCGGATACTCGACGAACTGGACAAAGCGATCGCCG
CGGAAATGGATGCGACCATCGATTTTGCCGTCGAATCCAAGGCACCGCCGCTGGGCTCGATGTTCAAGGATGTCTACGCC
GCCGGCGAGCCCGAGCCTGAATCCGTCAGGGCCCGTATCGACCGTGTTCTTGCCAGGGATGACGCATGA

Upstream 100 bases:

>100_bases
CTGATCATCCTGGTCGCCGTAGCGATCGTCTCCCGGACGACGAAGCTCAAGCTTCTCTGATCGCGGAATCCCGAATCATG
AACCATAAAAGGAGGGACGC

Downstream 100 bases:

>100_bases
TGACTTCGATGACGTACAGAGACGCGCTCCGCAAGGCGCTGGACGACGCGATGACGGATGACAGCTCCATCGTCGTGATC
GGCGAGGAAGTGGGCCGCTA

Product: dehydrogenase E1 component

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MDAPVNTNLLELYRTMRRIRTFEERVGELFVRGQSAGSMLHLSIGEESSAAGVCAAMKPQDTFTTHHRGHGIFLARGADP
KRMMAEIGGKETGYCRGKGGSMHIADMALGHLGANAIVGGGIPAVIGAGLSSRHLKQDSVSIAFFGDGAMQQGILYESMN
MASLWNLPVLFVCINNQYGMGTRIDQATRNTAFDQRAKAFGLNGAVVDGLDVEEVQAAARWLVDEARAGKPGFLSVEVYR
FFGHARMDKSPYREEAEELEGRKKDPVLFARNKLIDTGIEEERILDELDKAIAAEMDATIDFAVESKAPPLGSMFKDVYA
AGEPEPESVRARIDRVLARDDA

Sequences:

>Translated_342_residues
MDAPVNTNLLELYRTMRRIRTFEERVGELFVRGQSAGSMLHLSIGEESSAAGVCAAMKPQDTFTTHHRGHGIFLARGADP
KRMMAEIGGKETGYCRGKGGSMHIADMALGHLGANAIVGGGIPAVIGAGLSSRHLKQDSVSIAFFGDGAMQQGILYESMN
MASLWNLPVLFVCINNQYGMGTRIDQATRNTAFDQRAKAFGLNGAVVDGLDVEEVQAAARWLVDEARAGKPGFLSVEVYR
FFGHARMDKSPYREEAEELEGRKKDPVLFARNKLIDTGIEEERILDELDKAIAAEMDATIDFAVESKAPPLGSMFKDVYA
AGEPEPESVRARIDRVLARDDA
>Mature_342_residues
MDAPVNTNLLELYRTMRRIRTFEERVGELFVRGQSAGSMLHLSIGEESSAAGVCAAMKPQDTFTTHHRGHGIFLARGADP
KRMMAEIGGKETGYCRGKGGSMHIADMALGHLGANAIVGGGIPAVIGAGLSSRHLKQDSVSIAFFGDGAMQQGILYESMN
MASLWNLPVLFVCINNQYGMGTRIDQATRNTAFDQRAKAFGLNGAVVDGLDVEEVQAAARWLVDEARAGKPGFLSVEVYR
FFGHARMDKSPYREEAEELEGRKKDPVLFARNKLIDTGIEEERILDELDKAIAAEMDATIDFAVESKAPPLGSMFKDVYA
AGEPEPESVRARIDRVLARDDA

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=330, Percent_Identity=32.4242424242424, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI4505685, Length=329, Percent_Identity=33.434650455927, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI291084742, Length=329, Percent_Identity=33.434650455927, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI291084744, Length=335, Percent_Identity=32.5373134328358, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI291084757, Length=329, Percent_Identity=30.6990881458967, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI11386135, Length=314, Percent_Identity=24.8407643312102, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI258645172, Length=314, Percent_Identity=24.8407643312102, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17536047, Length=333, Percent_Identity=34.5345345345345, Blast_Score=190, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI32564172, Length=333, Percent_Identity=34.5345345345345, Blast_Score=190, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI86563355, Length=315, Percent_Identity=23.8095238095238, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI86563357, Length=315, Percent_Identity=23.8095238095238, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321026, Length=332, Percent_Identity=36.4457831325301, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24639744, Length=339, Percent_Identity=34.2182890855457, Blast_Score=184, Evalue=8e-47,
Organism=Drosophila melanogaster, GI28571106, Length=339, Percent_Identity=34.2182890855457, Blast_Score=184, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24639740, Length=339, Percent_Identity=34.2182890855457, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24639748, Length=313, Percent_Identity=35.1437699680511, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24639746, Length=334, Percent_Identity=34.1317365269461, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI21355903, Length=318, Percent_Identity=23.5849056603774, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 37242; Mature: 37242

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAPVNTNLLELYRTMRRIRTFEERVGELFVRGQSAGSMLHLSIGEESSAAGVCAAMKPQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCEEEECCCC
DTFTTHHRGHGIFLARGADPKRMMAEIGGKETGYCRGKGGSMHIADMALGHLGANAIVGG
CCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCEEECCCCCEEHHHHHHHHCCCCCEECC
GIPAVIGAGLSSRHLKQDSVSIAFFGDGAMQQGILYESMNMASLWNLPVLFVCINNQYGM
CHHHHHHCCCCCCCCCCCCEEEEEECCCHHHCCCHHHCCCHHHHCCCCEEEEEECCCCCC
GTRIDQATRNTAFDQRAKAFGLNGAVVDGLDVEEVQAAARWLVDEARAGKPGFLSVEVYR
CCCHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEHHHH
FFGHARMDKSPYREEAEELEGRKKDPVLFARNKLIDTGIEEERILDELDKAIAAEMDATI
HHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCEE
DFAVESKAPPLGSMFKDVYAAGEPEPESVRARIDRVLARDDA
EEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDAPVNTNLLELYRTMRRIRTFEERVGELFVRGQSAGSMLHLSIGEESSAAGVCAAMKPQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCEEEECCCC
DTFTTHHRGHGIFLARGADPKRMMAEIGGKETGYCRGKGGSMHIADMALGHLGANAIVGG
CCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCEEECCCCCEEHHHHHHHHCCCCCEECC
GIPAVIGAGLSSRHLKQDSVSIAFFGDGAMQQGILYESMNMASLWNLPVLFVCINNQYGM
CHHHHHHCCCCCCCCCCCCEEEEEECCCHHHCCCHHHCCCHHHHCCCCEEEEEECCCCCC
GTRIDQATRNTAFDQRAKAFGLNGAVVDGLDVEEVQAAARWLVDEARAGKPGFLSVEVYR
CCCHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEHHHH
FFGHARMDKSPYREEAEELEGRKKDPVLFARNKLIDTGIEEERILDELDKAIAAEMDATI
HHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCEE
DFAVESKAPPLGSMFKDVYAAGEPEPESVRARIDRVLARDDA
EEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]