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The map label for this gene is pdhB [H]

Identifier: 150398232

GI number: 150398232

Start: 3186500

End: 3187477

Strand: Reverse

Name: pdhB [H]

Synonym: Smed_3038

Alternate gene names: 150398232

Gene position: 3187477-3186500 (Counterclockwise)

Preceding gene: 150398233

Following gene: 150398231

Centisome position: 84.28

GC content: 61.66

Gene sequence:

>978_bases
ATGATGACTTCGATGACGTACAGAGACGCGCTCCGCAAGGCGCTGGACGACGCGATGACGGATGACAGCTCCATCGTCGT
GATCGGCGAGGAAGTGGGCCGCTATGGCGGCGCATACGGGGTCACCAAGGATCTCATCAAGATACATGGGGCCGACCGAC
TGATCGATACGCCGATCTCCGAGCCGGCCATCGTCGGCACCGCTGTCGGTGCGGCGATGACGGGCCTGCGCCCGGTCGCA
GAACTGATGTATATCGACTTTCTGGGCATGACCATGGATCAGCTCGCCAACCAGGCGGCCAAGATACGGTACATGTTCGG
CGGCCAGATCGGCGTACCTATGGTGCTCAGAACCCAGGGCGGTACGGGCCGTTCCGCTGGCGCACAGCATTCGCAGAGCC
TCGAAGCCTGGGTGATGCATACGCCAGGTCTTCGGCTCGCCATGCCGGCAACGGTCGCCGATGCCTATCATCTGCTGCGC
CAGAGCCTGACCAAACCGGATCCGGTGGTCTTCATCGAGCACAAGGCACTTTATACCCGCAAGGAGGAAATCGACCTCGA
CGCAGATCCTTTGCCCTGGGGCAAGGCTGCCGTTCGCCGCCAAGGCGACGATCTCGTCATCGTCACCTATTCCCGACAGG
TGTTTTACGCATTGGAAGCGGCCGACGCGCTTGCCAGGAAGGGGATCGAAGCGACCGTCATCGATCTCCGGACGCTGAAT
CCCCTGGATTTCGATACGGTGCGCGAGCATGTCGAGCGCGTAGGCAAGGCGATGGTGGTGAGCGAAGGGGTGATGACCTC
GGGCGTTGCCGCCGAGCTTGCCGCCCGCATCTCCGAGGAGTGTTTCGATTTTCTGGAACAGCCCGTCCTGCGCGTGGCCG
GTGAAGACATCCCGATCTCGGTTTCGCAGGAACTCGAGTCCGGCAGCGTGCCTTCCGCAAGGATGATTGCCGATGTTGCC
GCGAGAATGATGGCATGA

Upstream 100 bases:

>100_bases
CGCCGCTGGGCTCGATGTTCAAGGATGTCTACGCCGCCGGCGAGCCCGAGCCTGAATCCGTCAGGGCCCGTATCGACCGT
GTTCTTGCCAGGGATGACGC

Downstream 100 bases:

>100_bases
GCGAACGCATCCTCAAAATGCCTCGCCTCGGCGAGACGATGGAAGAAGGCAAGATTGTCGGCTGGCTGATCAAGCCGGGC
GACAGTTTCCGCCGCGGCGA

Product: transketolase central region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MMTSMTYRDALRKALDDAMTDDSSIVVIGEEVGRYGGAYGVTKDLIKIHGADRLIDTPISEPAIVGTAVGAAMTGLRPVA
ELMYIDFLGMTMDQLANQAAKIRYMFGGQIGVPMVLRTQGGTGRSAGAQHSQSLEAWVMHTPGLRLAMPATVADAYHLLR
QSLTKPDPVVFIEHKALYTRKEEIDLDADPLPWGKAAVRRQGDDLVIVTYSRQVFYALEAADALARKGIEATVIDLRTLN
PLDFDTVREHVERVGKAMVVSEGVMTSGVAAELAARISEECFDFLEQPVLRVAGEDIPISVSQELESGSVPSARMIADVA
ARMMA

Sequences:

>Translated_325_residues
MMTSMTYRDALRKALDDAMTDDSSIVVIGEEVGRYGGAYGVTKDLIKIHGADRLIDTPISEPAIVGTAVGAAMTGLRPVA
ELMYIDFLGMTMDQLANQAAKIRYMFGGQIGVPMVLRTQGGTGRSAGAQHSQSLEAWVMHTPGLRLAMPATVADAYHLLR
QSLTKPDPVVFIEHKALYTRKEEIDLDADPLPWGKAAVRRQGDDLVIVTYSRQVFYALEAADALARKGIEATVIDLRTLN
PLDFDTVREHVERVGKAMVVSEGVMTSGVAAELAARISEECFDFLEQPVLRVAGEDIPISVSQELESGSVPSARMIADVA
ARMMA
>Mature_325_residues
MMTSMTYRDALRKALDDAMTDDSSIVVIGEEVGRYGGAYGVTKDLIKIHGADRLIDTPISEPAIVGTAVGAAMTGLRPVA
ELMYIDFLGMTMDQLANQAAKIRYMFGGQIGVPMVLRTQGGTGRSAGAQHSQSLEAWVMHTPGLRLAMPATVADAYHLLR
QSLTKPDPVVFIEHKALYTRKEEIDLDADPLPWGKAAVRRQGDDLVIVTYSRQVFYALEAADALARKGIEATVIDLRTLN
PLDFDTVREHVERVGKAMVVSEGVMTSGVAAELAARISEECFDFLEQPVLRVAGEDIPISVSQELESGSVPSARMIADVA
ARMMA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=317, Percent_Identity=40.0630914826498, Blast_Score=248, Evalue=6e-66,
Organism=Homo sapiens, GI291084858, Length=317, Percent_Identity=38.1703470031546, Blast_Score=228, Evalue=4e-60,
Organism=Homo sapiens, GI4557353, Length=323, Percent_Identity=32.8173374613003, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI34101272, Length=323, Percent_Identity=32.8173374613003, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17538422, Length=319, Percent_Identity=41.3793103448276, Blast_Score=264, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17506935, Length=301, Percent_Identity=38.5382059800665, Blast_Score=178, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6319698, Length=328, Percent_Identity=39.3292682926829, Blast_Score=242, Evalue=6e-65,
Organism=Drosophila melanogaster, GI21358145, Length=312, Percent_Identity=44.2307692307692, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24650940, Length=312, Percent_Identity=44.2307692307692, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI160714832, Length=298, Percent_Identity=34.8993288590604, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI160714828, Length=298, Percent_Identity=34.8993288590604, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=50.5494505494505, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=50.5494505494505, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 35197; Mature: 35197

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTSMTYRDALRKALDDAMTDDSSIVVIGEEVGRYGGAYGVTKDLIKIHGADRLIDTPIS
CCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCCCCHHHHHHHHHCCCCHHCCCCCC
EPAIVGTAVGAAMTGLRPVAELMYIDFLGMTMDQLANQAAKIRYMFGGQIGVPMVLRTQG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECC
GTGRSAGAQHSQSLEAWVMHTPGLRLAMPATVADAYHLLRQSLTKPDPVVFIEHKALYTR
CCCCCCCCCHHHCCCEEEEECCCCEEECCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHH
KEEIDLDADPLPWGKAAVRRQGDDLVIVTYSRQVFYALEAADALARKGIEATVIDLRTLN
HHHCCCCCCCCCCCHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCC
PLDFDTVREHVERVGKAMVVSEGVMTSGVAAELAARISEECFDFLEQPVLRVAGEDIPIS
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
VSQELESGSVPSARMIADVAARMMA
HHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MMTSMTYRDALRKALDDAMTDDSSIVVIGEEVGRYGGAYGVTKDLIKIHGADRLIDTPIS
CCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCCCCHHHHHHHHHCCCCHHCCCCCC
EPAIVGTAVGAAMTGLRPVAELMYIDFLGMTMDQLANQAAKIRYMFGGQIGVPMVLRTQG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECC
GTGRSAGAQHSQSLEAWVMHTPGLRLAMPATVADAYHLLRQSLTKPDPVVFIEHKALYTR
CCCCCCCCCHHHCCCEEEEECCCCEEECCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHH
KEEIDLDADPLPWGKAAVRRQGDDLVIVTYSRQVFYALEAADALARKGIEATVIDLRTLN
HHHCCCCCCCCCCCHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCC
PLDFDTVREHVERVGKAMVVSEGVMTSGVAAELAARISEECFDFLEQPVLRVAGEDIPIS
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
VSQELESGSVPSARMIADVAARMMA
HHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]