Click here to switch to the map view.

The map label for this gene is leuD [H]

Identifier: 150398223

GI number: 150398223

Start: 3177289

End: 3177894

Strand: Direct

Name: leuD [H]

Synonym: Smed_3028

Alternate gene names: 150398223

Gene position: 3177289-3177894 (Clockwise)

Preceding gene: 150398222

Following gene: 150398224

Centisome position: 84.01

GC content: 60.07

Gene sequence:

>606_bases
ATGGACAAGTTCGTCAAGCTCACCGGCGTTGCCGCTCCCCTGCCCGTCGTCAACATCGACACGGACATGATCATTCCGAA
GGACTACCTGAAGACGATCAAACGCACCGGTCTTGGCACGGGTCTTTTCGCGGAAGCGCGCTACAACGAGGACGGCAGCC
CCAACCCGGATTTCGTGCTCAACAAGCCCGCCTACCAGAATGCAAAGATTCTCGTCGCCGGCGACAATTTCGGCTGTGGC
TCCTCGCGCGAGCATGCTCCCTGGGCTCTTCTCGATTTCGGCATCCGCTGCGTCATCTCGACCTCCTTCGCCGACATCTT
CTACAACAACTGTTTCAAGAACGGCATCTTGCCCATCGTCGTCAGCCAGGAAGACCTCGACAAGCTGATGGACGACGCGA
GCCGCGGCTCCAACGCCGTCTTGACGATCGATCTGGAAGCGCTGGAAATCACCGGGCCGGACGGCGGCTCGATCAAGTTC
GAGGTCGATGCCTTCAAGCGCCATTGCCTGCTGAACGGCCTCGACGATATCGGCCTCACGCTCGAAAAGGGTCGCTCCAT
CGACAATTTCGAGAAGGCGACCGCCGCCTCGCGCCCTTGGGCCTGA

Upstream 100 bases:

>100_bases
CCGCCCTGCTCCCTCTCGAAGAGAGCAGATTCTCCTTCATTTCGGCGCAAAACTGATCTAAACGCTTACCAACATTTCCG
CTGAAATCGAAGGCCAAGTC

Downstream 100 bases:

>100_bases
ACTGCCGGATGACGCGCAAGCTCATCTCGTCCGGTTCTCCCTTCGAAAGGACGGCAGGCTATTCGCGTGCCGTCGTGAAA
GGCGACTGGTGCTTCGTCTC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MDKFVKLTGVAAPLPVVNIDTDMIIPKDYLKTIKRTGLGTGLFAEARYNEDGSPNPDFVLNKPAYQNAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPIVVSQEDLDKLMDDASRGSNAVLTIDLEALEITGPDGGSIKF
EVDAFKRHCLLNGLDDIGLTLEKGRSIDNFEKATAASRPWA

Sequences:

>Translated_201_residues
MDKFVKLTGVAAPLPVVNIDTDMIIPKDYLKTIKRTGLGTGLFAEARYNEDGSPNPDFVLNKPAYQNAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPIVVSQEDLDKLMDDASRGSNAVLTIDLEALEITGPDGGSIKF
EVDAFKRHCLLNGLDDIGLTLEKGRSIDNFEKATAASRPWA
>Mature_201_residues
MDKFVKLTGVAAPLPVVNIDTDMIIPKDYLKTIKRTGLGTGLFAEARYNEDGSPNPDFVLNKPAYQNAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPIVVSQEDLDKLMDDASRGSNAVLTIDLEALEITGPDGGSIKF
EVDAFKRHCLLNGLDDIGLTLEKGRSIDNFEKATAASRPWA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=197, Percent_Identity=49.746192893401, Blast_Score=201, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6321429, Length=198, Percent_Identity=53.030303030303, Blast_Score=201, Evalue=9e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 21873; Mature: 21873

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFVKLTGVAAPLPVVNIDTDMIIPKDYLKTIKRTGLGTGLFAEARYNEDGSPNPDFVL
CCCEEEEECCCCCCCEEECCCCEECCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEE
NKPAYQNAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPIV
CCCCCCCCEEEEECCCCCCCCCCCCCCEEHHHHCEEEEEEHHHHHHHHHHHHHCCCEEEE
VSQEDLDKLMDDASRGSNAVLTIDLEALEITGPDGGSIKFEVDAFKRHCLLNGLDDIGLT
ECHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCEE
LEKGRSIDNFEKATAASRPWA
EECCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MDKFVKLTGVAAPLPVVNIDTDMIIPKDYLKTIKRTGLGTGLFAEARYNEDGSPNPDFVL
CCCEEEEECCCCCCCEEECCCCEECCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEE
NKPAYQNAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPIV
CCCCCCCCEEEEECCCCCCCCCCCCCCEEHHHHCEEEEEEHHHHHHHHHHHHHCCCEEEE
VSQEDLDKLMDDASRGSNAVLTIDLEALEITGPDGGSIKFEVDAFKRHCLLNGLDDIGLT
ECHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCEE
LEKGRSIDNFEKATAASRPWA
EECCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA