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The map label for this gene is gatA2 [H]

Identifier: 150398181

GI number: 150398181

Start: 3132426

End: 3133733

Strand: Direct

Name: gatA2 [H]

Synonym: Smed_2984

Alternate gene names: 150398181

Gene position: 3132426-3133733 (Clockwise)

Preceding gene: 150398180

Following gene: 150398183

Centisome position: 82.83

GC content: 67.97

Gene sequence:

>1308_bases
TTGCCCCGGCCCGACATGCCCTCCCGCGACCGCCTCGAAGCCATTCTTGCCGGCCTCGACCTTCGCCGGCACGAAGAGCG
CGCCTTCGTGAAACTCTATCCGGAGACGGCCCGCGCGGAAGCGGACGCCGCCGACCGGCGGCGGCGCGAGAAACGGAGCC
TCGGACTCCTCGACGGGCGGATCGTCTCCGTCAAGGACCTCTTCGACATAGCCGGCGAACCGACGCTCGCAGGCTCGATC
ATCCGCCGCGCAGCGCCGTCCGCAACGGCGGATGCGGCAATCGTCCGGCGTCTGCGTGCTGCCGGCGCGGTCATCATCGG
CAAGACGCATATGACGGAATTCGCCTTCACCGCGGTGGGCCTCAATCCGCATTATCCGGTGCCCGGCAATGCGACCGATC
GCAGCCTCATTCCCGGGGGGTCCTCGTCGGGCGCTGCAGTCTCAGCGGCAGAGGGCACGAGCGAGATCGCCATCGGCTCG
GACAGCGGCGGCTCGGTGCGCATTCCGGCTGCTCTGCAGGGCCTCGTCGGCTTCAAGCCGACCGCCCGCCGCATACCTCT
GGAAGGCGCCTTCCCGCTTTCCCCCAGCCTCGATTCCATCGGCCCGCTCGCGCGCACGGTCGCAGACTGCGCGGCGGCCG
ATGCGATCATGGCCGGCGAGACGCCGAGACCGCTCGAACCCGTTCCGCTCGCCGGCATGAAGCTCGGCATGCCCAAAGGC
GCCCTTCTGGAAGGGCTTGCAACGGAAATCGCGGCGGCTTTCGAAAGAAGCCTGCAAGCACTCTCCCGCGCGGGTGCTAA
ACTCGCCGAATGCGGGATCGACGATCTCCTCACCCGCTTCGCCGAGGCAACCGCGATCGGCTCGCTTGCCGGCCTCGAGG
CAAGTCGCGTACACGCCGACTGGCTCCGGGACGAGAATGCGCCGGTCGACATCCGCTTGAAATTCCCCCTACGCCGCCGG
CTCGCCATTCCCGACAGCGCGATCCACGACCTGCTGCGGACGCGGCAAGGGCTCATGCGCGCCATGGACGAGCGGCTGAG
ACCCTTCGATTTCATCCTGATGCCGGCCACACCCATCCCCGCCGTCAGCATCGCCTCGGTCGAAGAGGACAGGGCCGAAT
ACCGGCGTGTGGAAGACCTGCTGCTGCGCAACACGCAGGTCGCCAACCAGTTCGACCTGACCGCGATAACCCTGCCGATG
CCAGGCATGAGCCTGCCGGCAGGGCTGATGCTCATGGGCCGCAACGGAACGGACGGAGCGCTTCTACGGCTTGCCGCCTC
GGTGGAGCGATTGTTGCAGAGGGGCTGA

Upstream 100 bases:

>100_bases
GTCATCATCGCGCTGCCTTGGACGAAGACGGCCTTGCACCGCTCGCCGGTTCGGCAATCGCGCTCCGGCGGCAATCGCCA
CAATTCACGAGGTTTTCTGC

Downstream 100 bases:

>100_bases
CGCCTCAATGCACCGATGCGCTCGACCATTCCTCTTTGAGAATACGAAATGCATCTTCGAGCGCGGCAACCAGGACATCG
GTCAGCTCGCCGCCGTCATT

Product: amidase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MPRPDMPSRDRLEAILAGLDLRRHEERAFVKLYPETARAEADAADRRRREKRSLGLLDGRIVSVKDLFDIAGEPTLAGSI
IRRAAPSATADAAIVRRLRAAGAVIIGKTHMTEFAFTAVGLNPHYPVPGNATDRSLIPGGSSSGAAVSAAEGTSEIAIGS
DSGGSVRIPAALQGLVGFKPTARRIPLEGAFPLSPSLDSIGPLARTVADCAAADAIMAGETPRPLEPVPLAGMKLGMPKG
ALLEGLATEIAAAFERSLQALSRAGAKLAECGIDDLLTRFAEATAIGSLAGLEASRVHADWLRDENAPVDIRLKFPLRRR
LAIPDSAIHDLLRTRQGLMRAMDERLRPFDFILMPATPIPAVSIASVEEDRAEYRRVEDLLLRNTQVANQFDLTAITLPM
PGMSLPAGLMLMGRNGTDGALLRLAASVERLLQRG

Sequences:

>Translated_435_residues
MPRPDMPSRDRLEAILAGLDLRRHEERAFVKLYPETARAEADAADRRRREKRSLGLLDGRIVSVKDLFDIAGEPTLAGSI
IRRAAPSATADAAIVRRLRAAGAVIIGKTHMTEFAFTAVGLNPHYPVPGNATDRSLIPGGSSSGAAVSAAEGTSEIAIGS
DSGGSVRIPAALQGLVGFKPTARRIPLEGAFPLSPSLDSIGPLARTVADCAAADAIMAGETPRPLEPVPLAGMKLGMPKG
ALLEGLATEIAAAFERSLQALSRAGAKLAECGIDDLLTRFAEATAIGSLAGLEASRVHADWLRDENAPVDIRLKFPLRRR
LAIPDSAIHDLLRTRQGLMRAMDERLRPFDFILMPATPIPAVSIASVEEDRAEYRRVEDLLLRNTQVANQFDLTAITLPM
PGMSLPAGLMLMGRNGTDGALLRLAASVERLLQRG
>Mature_434_residues
PRPDMPSRDRLEAILAGLDLRRHEERAFVKLYPETARAEADAADRRRREKRSLGLLDGRIVSVKDLFDIAGEPTLAGSII
RRAAPSATADAAIVRRLRAAGAVIIGKTHMTEFAFTAVGLNPHYPVPGNATDRSLIPGGSSSGAAVSAAEGTSEIAIGSD
SGGSVRIPAALQGLVGFKPTARRIPLEGAFPLSPSLDSIGPLARTVADCAAADAIMAGETPRPLEPVPLAGMKLGMPKGA
LLEGLATEIAAAFERSLQALSRAGAKLAECGIDDLLTRFAEATAIGSLAGLEASRVHADWLRDENAPVDIRLKFPLRRRL
AIPDSAIHDLLRTRQGLMRAMDERLRPFDFILMPATPIPAVSIASVEEDRAEYRRVEDLLLRNTQVANQFDLTAITLPMP
GMSLPAGLMLMGRNGTDGALLRLAASVERLLQRG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=476, Percent_Identity=27.7310924369748, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI195972892, Length=204, Percent_Identity=34.3137254901961, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=419, Percent_Identity=28.6396181384248, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=267, Percent_Identity=29.5880149812734, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=154, Percent_Identity=33.7662337662338, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=189, Percent_Identity=29.1005291005291, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71990152, Length=160, Percent_Identity=34.375, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=395, Percent_Identity=29.3670886075949, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323950, Length=159, Percent_Identity=35.2201257861635, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=158, Percent_Identity=33.5443037974684, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24648113, Length=466, Percent_Identity=28.1115879828326, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652985, Length=213, Percent_Identity=32.3943661971831, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19922090, Length=213, Percent_Identity=32.3943661971831, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24652981, Length=213, Percent_Identity=32.3943661971831, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24652983, Length=213, Percent_Identity=32.3943661971831, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI161078093, Length=417, Percent_Identity=25.8992805755396, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24644968, Length=227, Percent_Identity=28.6343612334802, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45550774, Length=208, Percent_Identity=27.4038461538462, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24648435, Length=208, Percent_Identity=27.4038461538462, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648437, Length=208, Percent_Identity=27.4038461538462, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648441, Length=153, Percent_Identity=30.0653594771242, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648439, Length=153, Percent_Identity=30.0653594771242, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21356731, Length=188, Percent_Identity=32.4468085106383, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 46321; Mature: 46190

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRPDMPSRDRLEAILAGLDLRRHEERAFVKLYPETARAEADAADRRRREKRSLGLLDGR
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCE
IVSVKDLFDIAGEPTLAGSIIRRAAPSATADAAIVRRLRAAGAVIIGKTHMTEFAFTAVG
EEEHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECHHHHHHEEEEEC
LNPHYPVPGNATDRSLIPGGSSSGAAVSAAEGTSEIAIGSDSGGSVRIPAALQGLVGFKP
CCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCEECHHHHHHHHCCCC
TARRIPLEGAFPLSPSLDSIGPLARTVADCAAADAIMAGETPRPLEPVPLAGMKLGMPKG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCH
ALLEGLATEIAAAFERSLQALSRAGAKLAECGIDDLLTRFAEATAIGSLAGLEASRVHAD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
WLRDENAPVDIRLKFPLRRRLAIPDSAIHDLLRTRQGLMRAMDERLRPFDFILMPATPIP
HHCCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
AVSIASVEEDRAEYRRVEDLLLRNTQVANQFDLTAITLPMPGMSLPAGLMLMGRNGTDGA
CEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCCCCHH
LLRLAASVERLLQRG
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
PRPDMPSRDRLEAILAGLDLRRHEERAFVKLYPETARAEADAADRRRREKRSLGLLDGR
CCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCE
IVSVKDLFDIAGEPTLAGSIIRRAAPSATADAAIVRRLRAAGAVIIGKTHMTEFAFTAVG
EEEHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECHHHHHHEEEEEC
LNPHYPVPGNATDRSLIPGGSSSGAAVSAAEGTSEIAIGSDSGGSVRIPAALQGLVGFKP
CCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCEECHHHHHHHHCCCC
TARRIPLEGAFPLSPSLDSIGPLARTVADCAAADAIMAGETPRPLEPVPLAGMKLGMPKG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCH
ALLEGLATEIAAAFERSLQALSRAGAKLAECGIDDLLTRFAEATAIGSLAGLEASRVHAD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
WLRDENAPVDIRLKFPLRRRLAIPDSAIHDLLRTRQGLMRAMDERLRPFDFILMPATPIP
HHCCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
AVSIASVEEDRAEYRRVEDLLLRNTQVANQFDLTAITLPMPGMSLPAGLMLMGRNGTDGA
CEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCCCCHH
LLRLAASVERLLQRG
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]