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The map label for this gene is ygeZ [C]

Identifier: 150398180

GI number: 150398180

Start: 3130780

End: 3132213

Strand: Direct

Name: ygeZ [C]

Synonym: Smed_2983

Alternate gene names: 150398180

Gene position: 3130780-3132213 (Clockwise)

Preceding gene: 150398179

Following gene: 150398181

Centisome position: 82.78

GC content: 61.58

Gene sequence:

>1434_bases
ATGCGCTTCGATAAGGTCATCCGTTATGGAACGGTCGTCACCGCCAGCGATACATTCAAGAGCGATGTCGGCATTAAGGA
AGGCCGCATCGCGGCGCTTGCGGGGGCGTTGACGGAGGCGGACGAGATCATCGACGCAACCGGCCTCTACGTCATGCCCG
GCGGCATAGACAGCCATGTTCATCTGGACCAGCCCTCCGGTGAGGGCATCGTCATGGCCGACGACTTCGAAAGCGGTACC
CGTTCGGCGGCGATCGGCGGAAACAGCACGGTTCTGACCTTCTGCATGCAGGAGAAGGGCCAGAGCCTTCGCGAGGCACT
GAGACTTTACCACGCCAAAGCGGAGGGACGCTGCCACATCGACGTATCCTTCCACCTGGTCGTCACCGATCCTACGCCCG
AGGTGCTCGGGCAGGAACTGCCGGCGCTCGTCGCCGACGGCTACACCTCGATCAAGGTCTTCATGACCTATGACGGTCTG
CGGCTGCGCGACGACGAAATCCTCGCCACGCTCGACGCGGCGCGCCGAACCGGCGCATTGGTCATGGTTCACTGCGAGAA
CGAGGACGCGATCCGCTATCTGATCGGGCGCCACGAGGCCGAAGGACAGGTCGAACCCAGATACCATGCAGCCACTCGGC
CGATTGCCGCCGAGCGCGAGGCAACGCACCGGGCTTTGTCGCTCGCCGAGATTGTCGACACGCCTGTGGTCATCGTTCAT
GTTTCCAATCGTCAGGCGATGGAAGAGATCAGGCGGGCGCGGCAACGCGGCCAGAAGATCGCCGGCGAAACATGCCCGCA
ATATCTGATGCTGACTTCCGAGGATCTGGATGCCGACGCCCTTGAAGGAGCGAAATATGTCTGCTCGCCGCCGCCTCGGG
ACAAGGAAAGCCAATCGGCATGCTGGGAGGGCATTGAGCAAGGCGTGTTCGATCTCTTTTCTTCGGATCACTGCCCGTTT
CGCTTCGACGATCCCGCAGGCAAGCTGAACGAGAAAGGCAGACGGCACTTCCGCTGGATACCCAACGGCATCCCGGGTGT
CGCTACGCGGCTGCCTATCCTCTTCTCGGAAGGCGTGATGAAGGGACGGATCGATCTGAATCGCTTCGTCGCGGTAACAT
CGACCAACCACGCAAAGCTCTACGGACTCTATCCGCGCAAGGGCACGATCGCGATCGGAGCCGATGCCGACATCGCACTC
TGGGATCCGGATATGCAAATCACGCTGACGAACGAGATGCTTCGGCATGGGGCCGATTATACGCCCTATGAGGGCCTCGC
CGTTCGCGGCTGGCCGGTTCGGGTCATGGTCCGAGGCACCACGGTCGCCAAAGACGGGCATCTGTGTGATAGCCGACGCG
GCGGATCCTATCTGCACAGGACGCTCTCCTCGTTGACGAGGAATGCAGGCAACGGGAACCGTAACCGCGGTTAG

Upstream 100 bases:

>100_bases
AAGTGGATCGTCTCACGGCCGCCTTCGACCGGGTTCTTTCGACCGGCACCGGAAACACAGCCGATGCAAGGCGCGCCGGA
TGAGCCAGAAAGGGCCGGCC

Downstream 100 bases:

>100_bases
AGCAATTCCAGGAAAAGTGCGAAGCGCTTTTCCGTCCGGAATCGATCGCGGATAAGAGATTTTCGGAGAACAGCGGAAAT
GCTCTGACCTTTCGAAGAAG

Product: dihydropyrimidinase

Products: 3-ureidopropanoate

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MRFDKVIRYGTVVTASDTFKSDVGIKEGRIAALAGALTEADEIIDATGLYVMPGGIDSHVHLDQPSGEGIVMADDFESGT
RSAAIGGNSTVLTFCMQEKGQSLREALRLYHAKAEGRCHIDVSFHLVVTDPTPEVLGQELPALVADGYTSIKVFMTYDGL
RLRDDEILATLDAARRTGALVMVHCENEDAIRYLIGRHEAEGQVEPRYHAATRPIAAEREATHRALSLAEIVDTPVVIVH
VSNRQAMEEIRRARQRGQKIAGETCPQYLMLTSEDLDADALEGAKYVCSPPPRDKESQSACWEGIEQGVFDLFSSDHCPF
RFDDPAGKLNEKGRRHFRWIPNGIPGVATRLPILFSEGVMKGRIDLNRFVAVTSTNHAKLYGLYPRKGTIAIGADADIAL
WDPDMQITLTNEMLRHGADYTPYEGLAVRGWPVRVMVRGTTVAKDGHLCDSRRGGSYLHRTLSSLTRNAGNGNRNRG

Sequences:

>Translated_477_residues
MRFDKVIRYGTVVTASDTFKSDVGIKEGRIAALAGALTEADEIIDATGLYVMPGGIDSHVHLDQPSGEGIVMADDFESGT
RSAAIGGNSTVLTFCMQEKGQSLREALRLYHAKAEGRCHIDVSFHLVVTDPTPEVLGQELPALVADGYTSIKVFMTYDGL
RLRDDEILATLDAARRTGALVMVHCENEDAIRYLIGRHEAEGQVEPRYHAATRPIAAEREATHRALSLAEIVDTPVVIVH
VSNRQAMEEIRRARQRGQKIAGETCPQYLMLTSEDLDADALEGAKYVCSPPPRDKESQSACWEGIEQGVFDLFSSDHCPF
RFDDPAGKLNEKGRRHFRWIPNGIPGVATRLPILFSEGVMKGRIDLNRFVAVTSTNHAKLYGLYPRKGTIAIGADADIAL
WDPDMQITLTNEMLRHGADYTPYEGLAVRGWPVRVMVRGTTVAKDGHLCDSRRGGSYLHRTLSSLTRNAGNGNRNRG
>Mature_477_residues
MRFDKVIRYGTVVTASDTFKSDVGIKEGRIAALAGALTEADEIIDATGLYVMPGGIDSHVHLDQPSGEGIVMADDFESGT
RSAAIGGNSTVLTFCMQEKGQSLREALRLYHAKAEGRCHIDVSFHLVVTDPTPEVLGQELPALVADGYTSIKVFMTYDGL
RLRDDEILATLDAARRTGALVMVHCENEDAIRYLIGRHEAEGQVEPRYHAATRPIAAEREATHRALSLAEIVDTPVVIVH
VSNRQAMEEIRRARQRGQKIAGETCPQYLMLTSEDLDADALEGAKYVCSPPPRDKESQSACWEGIEQGVFDLFSSDHCPF
RFDDPAGKLNEKGRRHFRWIPNGIPGVATRLPILFSEGVMKGRIDLNRFVAVTSTNHAKLYGLYPRKGTIAIGADADIAL
WDPDMQITLTNEMLRHGADYTPYEGLAVRGWPVRVMVRGTTVAKDGHLCDSRRGGSYLHRTLSSLTRNAGNGNRNRG

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin. Has no activity on dihydropyrimidines [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503051, Length=467, Percent_Identity=32.5481798715203, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI62422571, Length=461, Percent_Identity=32.3210412147505, Blast_Score=238, Evalue=9e-63,
Organism=Homo sapiens, GI4503375, Length=465, Percent_Identity=33.7634408602151, Blast_Score=238, Evalue=9e-63,
Organism=Homo sapiens, GI4503377, Length=461, Percent_Identity=32.3210412147505, Blast_Score=229, Evalue=5e-60,
Organism=Homo sapiens, GI4503379, Length=459, Percent_Identity=31.1546840958606, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI19923821, Length=448, Percent_Identity=33.7053571428571, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI190194363, Length=463, Percent_Identity=31.5334773218143, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI18105007, Length=400, Percent_Identity=25.75, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI87082175, Length=459, Percent_Identity=38.9978213507625, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1786722, Length=460, Percent_Identity=32.1739130434783, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17539558, Length=478, Percent_Identity=33.2635983263598, Blast_Score=247, Evalue=8e-66,
Organism=Caenorhabditis elegans, GI71989490, Length=463, Percent_Identity=33.6933045356371, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI86575075, Length=467, Percent_Identity=26.338329764454, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI193204318, Length=412, Percent_Identity=24.5145631067961, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322218, Length=482, Percent_Identity=28.0082987551867, Blast_Score=140, Evalue=4e-34,
Organism=Drosophila melanogaster, GI221377917, Length=462, Percent_Identity=36.1471861471861, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI17137462, Length=451, Percent_Identity=34.8115299334812, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24644287, Length=306, Percent_Identity=35.6209150326797, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24644289, Length=233, Percent_Identity=36.0515021459227, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24642586, Length=406, Percent_Identity=27.5862068965517, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI18859883, Length=454, Percent_Identity=25.1101321585903, Blast_Score=99, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.2

Molecular weight: Translated: 52457; Mature: 52457

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDKVIRYGTVVTASDTFKSDVGIKEGRIAALAGALTEADEIIDATGLYVMPGGIDSHV
CCHHHEEHCCEEEEECCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCE
HLDQPSGEGIVMADDFESGTRSAAIGGNSTVLTFCMQEKGQSLREALRLYHAKAEGRCHI
EECCCCCCEEEEECCCCCCCCEEECCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCEEEE
DVSFHLVVTDPTPEVLGQELPALVADGYTSIKVFMTYDGLRLRDDEILATLDAARRTGAL
EEEEEEEEECCCHHHHHHHCCHHHHCCCCEEEEEEEECCCEECCHHHHHHHHHHHCCCCE
VMVHCENEDAIRYLIGRHEAEGQVEPRYHAATRPIAAEREATHRALSLAEIVDTPVVIVH
EEEEECCCCHHHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
VSNRQAMEEIRRARQRGQKIAGETCPQYLMLTSEDLDADALEGAKYVCSPPPRDKESQSA
ECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCCCCEECCCCCCCCCHHHH
CWEGIEQGVFDLFSSDHCPFRFDDPAGKLNEKGRRHFRWIPNGIPGVATRLPILFSEGVM
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCEEEECCCCCCCHHHHCCHHHHCCCE
KGRIDLNRFVAVTSTNHAKLYGLYPRKGTIAIGADADIALWDPDMQITLTNEMLRHGADY
ECCCCEEEEEEEEECCCEEEEEECCCCCEEEECCCCEEEEECCCCEEEEEHHHHHCCCCC
TPYEGLAVRGWPVRVMVRGTTVAKDGHLCDSRRGGSYLHRTLSSLTRNAGNGNRNRG
CCCCCEEECCCEEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRFDKVIRYGTVVTASDTFKSDVGIKEGRIAALAGALTEADEIIDATGLYVMPGGIDSHV
CCHHHEEHCCEEEEECCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCE
HLDQPSGEGIVMADDFESGTRSAAIGGNSTVLTFCMQEKGQSLREALRLYHAKAEGRCHI
EECCCCCCEEEEECCCCCCCCEEECCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCEEEE
DVSFHLVVTDPTPEVLGQELPALVADGYTSIKVFMTYDGLRLRDDEILATLDAARRTGAL
EEEEEEEEECCCHHHHHHHCCHHHHCCCCEEEEEEEECCCEECCHHHHHHHHHHHCCCCE
VMVHCENEDAIRYLIGRHEAEGQVEPRYHAATRPIAAEREATHRALSLAEIVDTPVVIVH
EEEEECCCCHHHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
VSNRQAMEEIRRARQRGQKIAGETCPQYLMLTSEDLDADALEGAKYVCSPPPRDKESQSA
ECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCCCCEECCCCCCCCCHHHH
CWEGIEQGVFDLFSSDHCPFRFDDPAGKLNEKGRRHFRWIPNGIPGVATRLPILFSEGVM
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCEEEECCCCCCCHHHHCCHHHHCCCE
KGRIDLNRFVAVTSTNHAKLYGLYPRKGTIAIGADADIALWDPDMQITLTNEMLRHGADY
ECCCCEEEEEEEEECCCEEEEEECCCCCEEEECCCCEEEEECCCCEEEEEHHHHHCCCCC
TPYEGLAVRGWPVRVMVRGTTVAKDGHLCDSRRGGSYLHRTLSSLTRNAGNGNRNRG
CCCCCEEECCCEEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,6-dihydrouracil; H2O

Specific reaction: 5,6-dihydrouracil + H2O = 3-ureidopropanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7765480 [H]