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The map label for this gene is ppa [H]

Identifier: 150398171

GI number: 150398171

Start: 3120881

End: 3121480

Strand: Reverse

Name: ppa [H]

Synonym: Smed_2974

Alternate gene names: 150398171

Gene position: 3121480-3120881 (Counterclockwise)

Preceding gene: 150398172

Following gene: 150398169

Centisome position: 82.54

GC content: 56.33

Gene sequence:

>600_bases
ATGTTGCATCGCGGCATGTTTCCCTTTATCCGACGGGGCGACTTAGAAACGATCTTGGAGGTGCTCATGCGGATCGACGC
GATTTCCATCGGAAAGAATCCACCGGAAGATGTCAACGTGATCGTGGAAGTCCCGGTCGGCGGGCATCCGATCAAATATG
AGATGGACAAGGAGGCTGGCACCCTTGTGGTCGATCGCTTCCTCTATACGCCGATGACCTATCCCGGCAATTACGGCTTC
GTGCCGCACACCCTTTCCGATGACGGCGATCCGATCGACGTGCTGATCTGCAATACCCGTCCGCTCGTCCCGGGCTGTGT
CATCAATGTCCGTCCTATCGGCGTAATGATGATGGAAGACAATTCCGGCCAGGACGAGAAGATCATCGCGGTGCCCTCCG
CCCACCTCACCAAGCGATACGACAAGGTGCACAGCTATACCGACCTGCCGGAAATCACGCTCAAGCAGATCGAGCACTTT
TTCGAGCACTACAAGGATCTGGAGCCCGGCAAGTGGGTGAAGATCTTCGGCTGGAAGGATGCGGCCATGGCCAAGCAGCT
GATCAGTGAAGCGGTCGAGCGCGCCAAGACGAAGAAATAA

Upstream 100 bases:

>100_bases
TTTCGGGCGCATCTTCTGCTCCCGGAATAAAATATCGGCTCCGCCCCTTTTTGGCCGTGCGGCCTGCCTACAGCAAATCC
GCCCGTCCGGAACCAAAGTC

Downstream 100 bases:

>100_bases
GAACGGTCCTGTTTCGCTGAGACAGGGAAGAACCTTCATAATCCTCCGGATCGGCCGCGATCCGGAGGGTTTTTTTGTTG
GGTTTCACCAGCCGCGACAT

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MLHRGMFPFIRRGDLETILEVLMRIDAISIGKNPPEDVNVIVEVPVGGHPIKYEMDKEAGTLVVDRFLYTPMTYPGNYGF
VPHTLSDDGDPIDVLICNTRPLVPGCVINVRPIGVMMMEDNSGQDEKIIAVPSAHLTKRYDKVHSYTDLPEITLKQIEHF
FEHYKDLEPGKWVKIFGWKDAAMAKQLISEAVERAKTKK

Sequences:

>Translated_199_residues
MLHRGMFPFIRRGDLETILEVLMRIDAISIGKNPPEDVNVIVEVPVGGHPIKYEMDKEAGTLVVDRFLYTPMTYPGNYGF
VPHTLSDDGDPIDVLICNTRPLVPGCVINVRPIGVMMMEDNSGQDEKIIAVPSAHLTKRYDKVHSYTDLPEITLKQIEHF
FEHYKDLEPGKWVKIFGWKDAAMAKQLISEAVERAKTKK
>Mature_199_residues
MLHRGMFPFIRRGDLETILEVLMRIDAISIGKNPPEDVNVIVEVPVGGHPIKYEMDKEAGTLVVDRFLYTPMTYPGNYGF
VPHTLSDDGDPIDVLICNTRPLVPGCVINVRPIGVMMMEDNSGQDEKIIAVPSAHLTKRYDKVHSYTDLPEITLKQIEHF
FEHYKDLEPGKWVKIFGWKDAAMAKQLISEAVERAKTKK

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=176, Percent_Identity=53.9772727272727, Blast_Score=204, Evalue=2e-54,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 22563; Mature: 22563

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHRGMFPFIRRGDLETILEVLMRIDAISIGKNPPEDVNVIVEVPVGGHPIKYEMDKEAG
CCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEECCCCC
TLVVDRFLYTPMTYPGNYGFVPHTLSDDGDPIDVLICNTRPLVPGCVINVRPIGVMMMED
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEEEEEEEEEC
NSGQDEKIIAVPSAHLTKRYDKVHSYTDLPEITLKQIEHFFEHYKDLEPGKWVKIFGWKD
CCCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCH
AAMAKQLISEAVERAKTKK
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLHRGMFPFIRRGDLETILEVLMRIDAISIGKNPPEDVNVIVEVPVGGHPIKYEMDKEAG
CCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEECCCCC
TLVVDRFLYTPMTYPGNYGFVPHTLSDDGDPIDVLICNTRPLVPGCVINVRPIGVMMMED
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEEEEEEEEEC
NSGQDEKIIAVPSAHLTKRYDKVHSYTDLPEITLKQIEHFFEHYKDLEPGKWVKIFGWKD
CCCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCH
AAMAKQLISEAVERAKTKK
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]