Click here to switch to the map view.

The map label for this gene is afr [H]

Identifier: 150398149

GI number: 150398149

Start: 3101367

End: 3102467

Strand: Direct

Name: afr [H]

Synonym: Smed_2952

Alternate gene names: 150398149

Gene position: 3101367-3102467 (Clockwise)

Preceding gene: 150398148

Following gene: 150398150

Centisome position: 82.01

GC content: 63.94

Gene sequence:

>1101_bases
ATGGAAAGACTCGGTATCGGCATCATCGGCTGCGGCAACATTTCGTCCGCCTATCTGAAGGCTATGGCAGCCTTTCCCAT
CCTCGACATTCGCGGCATCGCCGACCTGAACCGCAGCCTCGCCGAGGAGCGCGCCAACGAGTTCGGCGTCAGGGCGCGTG
ACCTTGCCGATCTCCTCGCAGACCCTTCGATCGAACTCATCGTCAACCTGACCGTGCCGAAGGCGCACGTCACCGTCGCC
ATGCAGGCGCTGGAGGCGGGGAAGCACACCTATTCCGAAAAGCCGCTCGCCACCGATTTCGCCGACGGACGCAAGCTTGC
GGCACTGGCCCGGGAAAAGGGCCTGCGCATCGGCGCCGCCCCGGACACATTTCTCGGCGGCTCGCATCAGACGGCGCGGG
CGATCGTCGATTCCGGCGCGCTCGGCATTCCAGTCGGCGGCACCGCCACCTTCATGTGTCCGGGCCATGAGCGCTGGCAT
CCCAATCCGGATTTCTACTATGACGTCGGCGGCGGGCCGATGCTCGACATGGGACCTTATTACATCACCGACCTCGTCAA
CCTCCTCGGCCCGGTCGCAAAGGTCGCCGGCTTCGCAACGTCGGCTCGCAAGGAGCGCACCATCACCAGCCAGGCGCGCA
ACGGCGAAAGCATTCCCGTTCGAGTTCCCACCCATGTCGCCGGCGTGATGGCCTTCGAGAATGGTGCCGTCGTGCAAATA
GGCATGAGCTTCGACGTCGCCGGCCATAAGCATGTCCCGCTCGAACTCTACGGTACCGAGGGCACGCTGATCGTGCCGGA
TCCCAACCATTTCGGCGGCCAGGTCGAGCTTCTGAAAAAAGGGGGCGCCTTTGAGCTTAAGACGACGACCGAGCCCTATG
CCGACGGCAATTATCGCTCGATCGGCGTCGCAGACATGGCGCATGCCATTCGCTCGGGCCGGCCGCACCGCGCAAATGGA
GATCTCGCGCTTCACGTGCTGGAGGTGATGGAAGCGTTCCACAAGGCTGCCGAAAGCGGCACCACCGTCAGCATCGCTAC
AACCACCGAGCGTCCGGCACCCCTGTCGGAGTCGCTCGTCGACGGCCGCATCGGCCGATAA

Upstream 100 bases:

>100_bases
GCGCTCTGCGAACTCTACGAGTCCCCCTGGGGGACGACCGGACCGGCGCCGGCGCTTTGACGCTTCCGGCTTCTGCAAAT
TCCACATCGGGAGGAGACTT

Downstream 100 bases:

>100_bases
TTCATCAGGAGGAGTTCGACATGCGAGAGGCACTGATCGTCTGGGGTGGCTGGAGCGGGCATGAGCCGCAGGAATGCGCC
CATATCATCCGCGACATGCT

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MERLGIGIIGCGNISSAYLKAMAAFPILDIRGIADLNRSLAEERANEFGVRARDLADLLADPSIELIVNLTVPKAHVTVA
MQALEAGKHTYSEKPLATDFADGRKLAALAREKGLRIGAAPDTFLGGSHQTARAIVDSGALGIPVGGTATFMCPGHERWH
PNPDFYYDVGGGPMLDMGPYYITDLVNLLGPVAKVAGFATSARKERTITSQARNGESIPVRVPTHVAGVMAFENGAVVQI
GMSFDVAGHKHVPLELYGTEGTLIVPDPNHFGGQVELLKKGGAFELKTTTEPYADGNYRSIGVADMAHAIRSGRPHRANG
DLALHVLEVMEAFHKAAESGTTVSIATTTERPAPLSESLVDGRIGR

Sequences:

>Translated_366_residues
MERLGIGIIGCGNISSAYLKAMAAFPILDIRGIADLNRSLAEERANEFGVRARDLADLLADPSIELIVNLTVPKAHVTVA
MQALEAGKHTYSEKPLATDFADGRKLAALAREKGLRIGAAPDTFLGGSHQTARAIVDSGALGIPVGGTATFMCPGHERWH
PNPDFYYDVGGGPMLDMGPYYITDLVNLLGPVAKVAGFATSARKERTITSQARNGESIPVRVPTHVAGVMAFENGAVVQI
GMSFDVAGHKHVPLELYGTEGTLIVPDPNHFGGQVELLKKGGAFELKTTTEPYADGNYRSIGVADMAHAIRSGRPHRANG
DLALHVLEVMEAFHKAAESGTTVSIATTTERPAPLSESLVDGRIGR
>Mature_366_residues
MERLGIGIIGCGNISSAYLKAMAAFPILDIRGIADLNRSLAEERANEFGVRARDLADLLADPSIELIVNLTVPKAHVTVA
MQALEAGKHTYSEKPLATDFADGRKLAALAREKGLRIGAAPDTFLGGSHQTARAIVDSGALGIPVGGTATFMCPGHERWH
PNPDFYYDVGGGPMLDMGPYYITDLVNLLGPVAKVAGFATSARKERTITSQARNGESIPVRVPTHVAGVMAFENGAVVQI
GMSFDVAGHKHVPLELYGTEGTLIVPDPNHFGGQVELLKKGGAFELKTTTEPYADGNYRSIGVADMAHAIRSGRPHRANG
DLALHVLEVMEAFHKAAESGTTVSIATTTERPAPLSESLVDGRIGR

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI1789848, Length=89, Percent_Identity=37.0786516853933, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 38795; Mature: 38795

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00147 ARGINASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLGIGIIGCGNISSAYLKAMAAFPILDIRGIADLNRSLAEERANEFGVRARDLADLLA
CCCCCEEEEECCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
DPSIELIVNLTVPKAHVTVAMQALEAGKHTYSEKPLATDFADGRKLAALAREKGLRIGAA
CCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEECCC
PDTFLGGSHQTARAIVDSGALGIPVGGTATFMCPGHERWHPNPDFYYDVGGGPMLDMGPY
CCCCCCCCCHHHHHHHCCCCCEECCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCCCH
YITDLVNLLGPVAKVAGFATSARKERTITSQARNGESIPVRVPTHVAGVMAFENGAVVQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHEEEEEECCCCEEEE
GMSFDVAGHKHVPLELYGTEGTLIVPDPNHFGGQVELLKKGGAFELKTTTEPYADGNYRS
ECEEECCCCCCCCEEEECCCCEEEECCCCCCCCEEEEEECCCCEEEEECCCCCCCCCEEE
IGVADMAHAIRSGRPHRANGDLALHVLEVMEAFHKAAESGTTVSIATTTERPAPLSESLV
ECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHH
DGRIGR
CCCCCC
>Mature Secondary Structure
MERLGIGIIGCGNISSAYLKAMAAFPILDIRGIADLNRSLAEERANEFGVRARDLADLLA
CCCCCEEEEECCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
DPSIELIVNLTVPKAHVTVAMQALEAGKHTYSEKPLATDFADGRKLAALAREKGLRIGAA
CCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEECCC
PDTFLGGSHQTARAIVDSGALGIPVGGTATFMCPGHERWHPNPDFYYDVGGGPMLDMGPY
CCCCCCCCCHHHHHHHCCCCCEECCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCCCH
YITDLVNLLGPVAKVAGFATSARKERTITSQARNGESIPVRVPTHVAGVMAFENGAVVQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHEEEEEECCCCEEEE
GMSFDVAGHKHVPLELYGTEGTLIVPDPNHFGGQVELLKKGGAFELKTTTEPYADGNYRS
ECEEECCCCCCCCEEEECCCCEEEECCCCCCCCEEEEEECCCCEEEEECCCCCCCCCEEE
IGVADMAHAIRSGRPHRANGDLALHVLEVMEAFHKAAESGTTVSIATTTERPAPLSESLV
ECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHH
DGRIGR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]