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The map label for this gene is sucB [H]

Identifier: 150398137

GI number: 150398137

Start: 3085800

End: 3087047

Strand: Reverse

Name: sucB [H]

Synonym: Smed_2940

Alternate gene names: 150398137

Gene position: 3087047-3085800 (Counterclockwise)

Preceding gene: 150398138

Following gene: 150398136

Centisome position: 81.63

GC content: 64.26

Gene sequence:

>1248_bases
ATGGCAACAGAAATCCGCGTTCCCACTCTCGGCGAATCCGTCAGTGAAGCGACCGTCGGCACCTGGTTCAAGAAGGTTGG
CGATGCGATCAAGGCAGACGAGCCGATCGTCGAGCTTGAAACCGACAAGGTGACGATCGAAGTTCCTGCACCGGCAGCCG
GCACGCTCAGCGAAATCGTTGCCCAGGCGGGCGAGACGGTCGGGCTAGGTGCCCTTCTCGGCCAGATTGCCGAAGGCGCG
GGTGCAGCTGCCGCCGCTCCGGCTGCGGCCGAGAAAAAACCCGAACCGGTTGCGGCTGCTCCGGCTGCGGCCGCGCGGCC
GCCCACTGCAGCCGCGCCGCAGACATCAACGTCGATGCCGCCGGCACCGGCATCCGCCAAGCTCATTGCCGAAAACAGCC
TTTCCGCCGATCAGATCGACGGCTCGGGTAAGCGCGGCCAGGTTCTGAAGGGCGACGTGCTCGCGGCCGTCGCCAAGGGC
ATTTCGGCCCCGGCTGCCGCCGAGCCGGCCCAGGTTCAGGCCCGCGCGCCGGCACCGGCTGAAGACGCGGCTCGCGAGGA
GCGCGTCAAGATGACCCGCCTGCGCCAGACGATTGCCCGTCGCCTGAAGGACGCGCAGAACACGGCGGCGATGCTTACCA
CCTATAACGAGGTGGATATGAGCGCCGTCATGAGCCTGCGTTCAAAGTACAAGGACATCTTCGAGAAGAAGCACGGCGTG
AAGCTCGGCTTCATGGGCTTCTTCACCAAGGCAGTGACCCACGCGCTGAAGGAACTGCCGGCTGTCAACGCCGAGATCGA
CGGCACCGAGATCATCTACAAGAATTTCTGCCATGTCGGCGTCGCGGTCGGCACCGACAAGGGTCTCGTCGTGCCGGTCG
TGCGTGACGCCGACCAGATGTCGATCGCCGAAATCGAGAAGGAGATCGGCCGTCTCGGTAAGGCAGCGCGCGACGGCGCT
CTGTCCATGGCCGACATGCAGGGCGGCACCTTCACCATCTCCAACGGCGGTGTGTACGGCTCACTGATGTCCTCGCCGAT
CCTCAATGCTCCGCAATCGGGCATCCTCGGCATGCACAAGATCCAGGACCGCCCGGTCGCGATCGGTGGTCAGGTCGTCA
TCCGGCCGATGATGTATCTGGCGCTGTCCTACGATCACCGTATCGTCGACGGCAAGGAAGCGGTGACCTTCCTCGTGCGC
GTCAAGGAAAGCCTCGAGGATCCGGAACGCCTGGTACTCGATCTCTAA

Upstream 100 bases:

>100_bases
AAGCATCTGGCGCAGCTTGCCGCCTTCCTCGAGGACGCGCTGGGGGGCTGACGAAGCCCCTCACGGTCTGAAACAGAAAT
CTGATCAACGGACAAAAAAC

Downstream 100 bases:

>100_bases
GGAATGGCGGGGGCCACGTGCCCCCGTTTCCATCTCCGCCGGTGCCCGTGCCGAGAACAGGCGTCCTACGGGCATCGACA
GCCACGGAGTCAGAGGTTAG

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MATEIRVPTLGESVSEATVGTWFKKVGDAIKADEPIVELETDKVTIEVPAPAAGTLSEIVAQAGETVGLGALLGQIAEGA
GAAAAAPAAAEKKPEPVAAAPAAAARPPTAAAPQTSTSMPPAPASAKLIAENSLSADQIDGSGKRGQVLKGDVLAAVAKG
ISAPAAAEPAQVQARAPAPAEDAAREERVKMTRLRQTIARRLKDAQNTAAMLTTYNEVDMSAVMSLRSKYKDIFEKKHGV
KLGFMGFFTKAVTHALKELPAVNAEIDGTEIIYKNFCHVGVAVGTDKGLVVPVVRDADQMSIAEIEKEIGRLGKAARDGA
LSMADMQGGTFTISNGGVYGSLMSSPILNAPQSGILGMHKIQDRPVAIGGQVVIRPMMYLALSYDHRIVDGKEAVTFLVR
VKESLEDPERLVLDL

Sequences:

>Translated_415_residues
MATEIRVPTLGESVSEATVGTWFKKVGDAIKADEPIVELETDKVTIEVPAPAAGTLSEIVAQAGETVGLGALLGQIAEGA
GAAAAAPAAAEKKPEPVAAAPAAAARPPTAAAPQTSTSMPPAPASAKLIAENSLSADQIDGSGKRGQVLKGDVLAAVAKG
ISAPAAAEPAQVQARAPAPAEDAAREERVKMTRLRQTIARRLKDAQNTAAMLTTYNEVDMSAVMSLRSKYKDIFEKKHGV
KLGFMGFFTKAVTHALKELPAVNAEIDGTEIIYKNFCHVGVAVGTDKGLVVPVVRDADQMSIAEIEKEIGRLGKAARDGA
LSMADMQGGTFTISNGGVYGSLMSSPILNAPQSGILGMHKIQDRPVAIGGQVVIRPMMYLALSYDHRIVDGKEAVTFLVR
VKESLEDPERLVLDL
>Mature_414_residues
ATEIRVPTLGESVSEATVGTWFKKVGDAIKADEPIVELETDKVTIEVPAPAAGTLSEIVAQAGETVGLGALLGQIAEGAG
AAAAAPAAAEKKPEPVAAAPAAAARPPTAAAPQTSTSMPPAPASAKLIAENSLSADQIDGSGKRGQVLKGDVLAAVAKGI
SAPAAAEPAQVQARAPAPAEDAAREERVKMTRLRQTIARRLKDAQNTAAMLTTYNEVDMSAVMSLRSKYKDIFEKKHGVK
LGFMGFFTKAVTHALKELPAVNAEIDGTEIIYKNFCHVGVAVGTDKGLVVPVVRDADQMSIAEIEKEIGRLGKAARDGAL
SMADMQGGTFTISNGGVYGSLMSSPILNAPQSGILGMHKIQDRPVAIGGQVVIRPMMYLALSYDHRIVDGKEAVTFLVRV
KESLEDPERLVLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=59.051724137931, Blast_Score=298, Evalue=8e-81,
Organism=Homo sapiens, GI203098753, Length=455, Percent_Identity=27.6923076923077, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI203098816, Length=455, Percent_Identity=27.6923076923077, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=27.039627039627, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI31711992, Length=436, Percent_Identity=29.8165137614679, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=36.6459627329193, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1786946, Length=414, Percent_Identity=49.5169082125604, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=31.5294117647059, Blast_Score=187, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI25146366, Length=414, Percent_Identity=44.2028985507246, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17560088, Length=446, Percent_Identity=32.0627802690583, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=29.0993071593533, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=30.1639344262295, Blast_Score=122, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=416, Percent_Identity=45.6730769230769, Blast_Score=356, Evalue=4e-99,
Organism=Saccharomyces cerevisiae, GI6324258, Length=459, Percent_Identity=26.3616557734205, Blast_Score=118, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24645909, Length=246, Percent_Identity=54.8780487804878, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI18859875, Length=418, Percent_Identity=27.9904306220096, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=32.034632034632, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=32.034632034632, Blast_Score=118, Evalue=7e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43290; Mature: 43159

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEIRVPTLGESVSEATVGTWFKKVGDAIKADEPIVELETDKVTIEVPAPAAGTLSEIV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEECCCCCHHHHHHH
AQAGETVGLGALLGQIAEGAGAAAAAPAAAEKKPEPVAAAPAAAARPPTAAAPQTSTSMP
HHCCCCCHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAPASAKLIAENSLSADQIDGSGKRGQVLKGDVLAAVAKGISAPAAAEPAQVQARAPAPA
CCCCCCEEEECCCCCHHHCCCCCCCCCEECHHHHHHHHHCCCCCCCCCCCCEEECCCCCH
EDAAREERVKMTRLRQTIARRLKDAQNTAAMLTTYNEVDMSAVMSLRSKYKDIFEKKHGV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
KLGFMGFFTKAVTHALKELPAVNAEIDGTEIIYKNFCHVGVAVGTDKGLVVPVVRDADQM
EEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEEEECCCCEEEEEECCCCHH
SIAEIEKEIGRLGKAARDGALSMADMQGGTFTISNGGVYGSLMSSPILNAPQSGILGMHK
HHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCEEHHHHCCCCCCCCCCCCCCHHH
IQDRPVAIGGQVVIRPMMYLALSYDHRIVDGKEAVTFLVRVKESLEDPERLVLDL
HCCCCEEECCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure 
ATEIRVPTLGESVSEATVGTWFKKVGDAIKADEPIVELETDKVTIEVPAPAAGTLSEIV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEECCCCCHHHHHHH
AQAGETVGLGALLGQIAEGAGAAAAAPAAAEKKPEPVAAAPAAAARPPTAAAPQTSTSMP
HHCCCCCHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAPASAKLIAENSLSADQIDGSGKRGQVLKGDVLAAVAKGISAPAAAEPAQVQARAPAPA
CCCCCCEEEECCCCCHHHCCCCCCCCCEECHHHHHHHHHCCCCCCCCCCCCEEECCCCCH
EDAAREERVKMTRLRQTIARRLKDAQNTAAMLTTYNEVDMSAVMSLRSKYKDIFEKKHGV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
KLGFMGFFTKAVTHALKELPAVNAEIDGTEIIYKNFCHVGVAVGTDKGLVVPVVRDADQM
EEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEEEECCCCEEEEEECCCCHH
SIAEIEKEIGRLGKAARDGALSMADMQGGTFTISNGGVYGSLMSSPILNAPQSGILGMHK
HHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCEEHHHHCCCCCCCCCCCCCCHHH
IQDRPVAIGGQVVIRPMMYLALSYDHRIVDGKEAVTFLVRVKESLEDPERLVLDL
HCCCCEEECCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]