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The map label for this gene is mak [H]

Identifier: 150398071

GI number: 150398071

Start: 3008857

End: 3009753

Strand: Reverse

Name: mak [H]

Synonym: Smed_2873

Alternate gene names: 150398071

Gene position: 3009753-3008857 (Counterclockwise)

Preceding gene: 150398072

Following gene: 150398070

Centisome position: 79.58

GC content: 63.21

Gene sequence:

>897_bases
ATGTTTATCGGAATTGACTGGGGCGGCACGAAAATGGAGGTCATCGCGCTTGATCGCGGAGGCGAGACGCGTGCGCGGCA
TCGCGTTCCAACGCCTACGAGCGGCTATGACGATTGCATCCGAGCCGTCGTGGAACTCGTCGCGGCGGCCGAAAGCACGG
CCGGCGAGCGCGGCTCGATCGGCATCGGCATACCCGGAAGCCCCAATCCGCGCACCGGCATCGTGCGCAATTCCAACGCA
GTGCTCATTAACGGAAAGCCGCTCGGGCGGGATCTCGAAGCGGCTTTCGGCCGTCCGGTGCGTCTTGCAAACGATGCCAA
TTGCCTCGCCGTGTCCGAAGCCGTGGACGGCGCGGGCAAGGACGCAAAACTCGTCTTCGGCGTCATCGTCGGAACGGGCC
ATGGCGGGGGGCTCGCGATCGATCGGAGGGTTCATGCCGGGTATCAGGGTATCGCCGCCGAGATCGGCCATTATCCTCTG
CCCTGGATGACCAGGGACGAATATCCGGGGCACAGGTGCTGGTGCGGCAAGCTCGGTTGCCTCGACATGTATGCCTGCGG
CACGGGACTTGAACTCGACTACCGCCTGACGACCGGCATGGAACGCCGCGGACGGGACATTATCGAAGCGAGCCGCTCGG
GCGATCCGGCGGCATCCGGTGTCTACGAGCGATTCGTCGACCGGTTGGCCCGCAGCCTCGCGCTCTTGACGAATATCGTC
GACCCCGACGTTTTCGTGCTTGGCGGGGGCATGTCCAATGTCGACGAGATCTACGCTGAACTGCCGGCATTGATTACGAA
ATATCTTTTCGGCGACAGTTTCGAAACGCCGATTCGCAAGGCAGTGCACGGCGACAGCTCCGGGGTGCGCGGCGCCGCAT
GGCTTTGGAAGGAATAG

Upstream 100 bases:

>100_bases
GGGCTAGCGCCATCAATGTCTCAAATGCTAAGCAGATGCCGCCTCCGCGGAGGCTGGCTCACCTTTCTTGACAGCATTTT
CATTCTAAAGATCCTGACAG

Downstream 100 bases:

>100_bases
AGAGTGTCGAGCCTCGGCGGGCGCGAGACATCCCATCGTACGGATCACGACAAGCGGAGGCATCATGAACATAGATAACG
ATCTGCGCCGGATTGCGCTG

Product: ROK family protein

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MFIGIDWGGTKMEVIALDRGGETRARHRVPTPTSGYDDCIRAVVELVAAAESTAGERGSIGIGIPGSPNPRTGIVRNSNA
VLINGKPLGRDLEAAFGRPVRLANDANCLAVSEAVDGAGKDAKLVFGVIVGTGHGGGLAIDRRVHAGYQGIAAEIGHYPL
PWMTRDEYPGHRCWCGKLGCLDMYACGTGLELDYRLTTGMERRGRDIIEASRSGDPAASGVYERFVDRLARSLALLTNIV
DPDVFVLGGGMSNVDEIYAELPALITKYLFGDSFETPIRKAVHGDSSGVRGAAWLWKE

Sequences:

>Translated_298_residues
MFIGIDWGGTKMEVIALDRGGETRARHRVPTPTSGYDDCIRAVVELVAAAESTAGERGSIGIGIPGSPNPRTGIVRNSNA
VLINGKPLGRDLEAAFGRPVRLANDANCLAVSEAVDGAGKDAKLVFGVIVGTGHGGGLAIDRRVHAGYQGIAAEIGHYPL
PWMTRDEYPGHRCWCGKLGCLDMYACGTGLELDYRLTTGMERRGRDIIEASRSGDPAASGVYERFVDRLARSLALLTNIV
DPDVFVLGGGMSNVDEIYAELPALITKYLFGDSFETPIRKAVHGDSSGVRGAAWLWKE
>Mature_298_residues
MFIGIDWGGTKMEVIALDRGGETRARHRVPTPTSGYDDCIRAVVELVAAAESTAGERGSIGIGIPGSPNPRTGIVRNSNA
VLINGKPLGRDLEAAFGRPVRLANDANCLAVSEAVDGAGKDAKLVFGVIVGTGHGGGLAIDRRVHAGYQGIAAEIGHYPL
PWMTRDEYPGHRCWCGKLGCLDMYACGTGLELDYRLTTGMERRGRDIIEASRSGDPAASGVYERFVDRLARSLALLTNIV
DPDVFVLGGGMSNVDEIYAELPALITKYLFGDSFETPIRKAVHGDSSGVRGAAWLWKE

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI4885285, Length=271, Percent_Identity=27.3062730627306, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI298566315, Length=271, Percent_Identity=27.3062730627306, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI190014632, Length=271, Percent_Identity=27.3062730627306, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI298566325, Length=271, Percent_Identity=27.3062730627306, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI87081733, Length=301, Percent_Identity=53.8205980066445, Blast_Score=321, Evalue=4e-89,
Organism=Escherichia coli, GI1787363, Length=299, Percent_Identity=40.8026755852843, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1786891, Length=275, Percent_Identity=25.0909090909091, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787878, Length=210, Percent_Identity=30.4761904761905, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790522, Length=211, Percent_Identity=29.3838862559242, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31785; Mature: 31785

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIGIDWGGTKMEVIALDRGGETRARHRVPTPTSGYDDCIRAVVELVAAAESTAGERGSI
CEEEEECCCCEEEEEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
GIGIPGSPNPRTGIVRNSNAVLINGKPLGRDLEAAFGRPVRLANDANCLAVSEAVDGAGK
EEECCCCCCCCCCEEECCCEEEECCCCCCCCHHHHCCCCEEECCCCCEEEEHHHHCCCCC
DAKLVFGVIVGTGHGGGLAIDRRVHAGYQGIAAEIGHYPLPWMTRDEYPGHRCWCGKLGC
CCEEEEEEEEECCCCCCEEEECHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCC
LDMYACGTGLELDYRLTTGMERRGRDIIEASRSGDPAASGVYERFVDRLARSLALLTNIV
EEHEECCCCCEEEEEECCCHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
DPDVFVLGGGMSNVDEIYAELPALITKYLFGDSFETPIRKAVHGDSSGVRGAAWLWKE
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCEEECCC
>Mature Secondary Structure
MFIGIDWGGTKMEVIALDRGGETRARHRVPTPTSGYDDCIRAVVELVAAAESTAGERGSI
CEEEEECCCCEEEEEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
GIGIPGSPNPRTGIVRNSNAVLINGKPLGRDLEAAFGRPVRLANDANCLAVSEAVDGAGK
EEECCCCCCCCCCEEECCCEEEECCCCCCCCHHHHCCCCEEECCCCCEEEEHHHHCCCCC
DAKLVFGVIVGTGHGGGLAIDRRVHAGYQGIAAEIGHYPLPWMTRDEYPGHRCWCGKLGC
CCEEEEEEEEECCCCCCEEEECHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCC
LDMYACGTGLELDYRLTTGMERRGRDIIEASRSGDPAASGVYERFVDRLARSLALLTNIV
EEHEECCCCCEEEEEECCCHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
DPDVFVLGGGMSNVDEIYAELPALITKYLFGDSFETPIRKAVHGDSSGVRGAAWLWKE
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]