Click here to switch to the map view.

The map label for this gene is alkJ [H]

Identifier: 150398067

GI number: 150398067

Start: 3001539

End: 3003134

Strand: Direct

Name: alkJ [H]

Synonym: Smed_2869

Alternate gene names: 150398067

Gene position: 3001539-3003134 (Clockwise)

Preceding gene: 150398066

Following gene: 150398074

Centisome position: 79.37

GC content: 63.78

Gene sequence:

>1596_bases
ATGGACACGTTCGACTACATCATCGTCGGAGCGGGCAGTGCCGGCTGCGTGCTCGCCAATCGCCTGTCGGAAAACCCGGA
TCGCCGCGTTCTGTTGCTGGAGGCCGGCGGCAGCGACAATTATCACTGGATCCACATTCCGGTCGGCTATCTCTATTGCA
TCAACAATCCCCGCACCGACTGGTGCTTCACCACGGCCGCGGAGGAAGGGCTCAACGGCCGGTCGCTCGGTTATCCTCGC
GGCAAGGTTCTCGGAGGCTGCTCCTCGATCAATGGAATGATCTACATGCGCGGGCAGGCGCGCGACTACGACCTCTGGCG
CCAGCTCGGCTGCGCCGGCTGGAGCTGGAATGACGTGTTGCCGCTCTTCAGGAAATGCGAGGACCACCATCGCGGCGCCG
ACGAAATGCACGGCGCAGGCGGCGAATGGCGGGTCGAGAAGGCGCGCGTCCGATGGGCCGTGCTCGACGCTTTCCAGAAG
GCCGCCACCGAAGCCGGCATCCCCGAGACAGACGACTTCAACCGCGGAACCAACGAGGGTTCGGGCTATTTCGACGTCAA
CCAGCGGTCCGGCATCCGCTGGAACACGGCCAAGGCATTTCTCAGGCCTGCGATGCGCCGACGCAATCTCACAATCCTCA
CGAAAGCCCATGTCCGCCGACTTGTTCTCAATGATCGACGTGTCTCCGGCGTCGAGTTCCAGCATGACGGGGTCACCAAA
AGCGTGCTGGCCCGTCGAGAGGTGGTGCTTTCCGCCGGCGCAATCGGTTCTCCGCACATCCTGGAACTCTCCGGCATCGG
CCGACCCGACGTGCTGCGCGAAAACGGCATCGAGGTCCGCCATGAGCTGCCCGCCGTCGGCGAGAACCTGCAGGACCATT
TGCAATTGCGCCTCGCCTACAAGGTCACGGGCGTGCCTACGCTCAACGAAAAAGCCACTTCGCTCTTCGGCAAGGCTGCG
ATCGGTCTCGAATATCTCGTCCGCCGCTCCGGCCCCATGGCAATGGCGCCGAGCCAGCTCGGCATCTTCACGCGCTCAGG
TCCGGAAAAGGAAACGCCGGATCTGCAGTATCATGTACAGCCGGTAACGCTCGAAAAGTTCGGTGAACCGGTGCATCCCT
TCCCCGCAATTACGGCAAGCGTCTGCAATCTGAGGCCGGAAAGCCGTGGTTCGGTGCATCTGAAGAGCCCGGATTTCGCC
GCGGCGCCGAATATCCGTCCGCGCTATCTCTCGGCCGAGGCCGACCGGGAGGTGGCCGTGAAGGCGATCCGGCTCACCCG
CCGCATCGTCTCGCAGCCCGCTTTCGCCCGCTACAAACCGGTCGAGTTCAAGCCCGGCCCGAGCTACGAAACGGATGAAG
ACCTGAAGCGCGCCGCGGGCGAGATCGGAACGACGATCTTCCACCCGGTAGGCACCTGCCGCATGGGGGGCGACCAGGCG
AGCGTCGTCGATCCGGAATTGCGGCTGCGCGGCCTCGCCGGCCTGCGGATCGCCGACGCGTCGATCATGCCGACCATCAC
CTCGGGCAACACCAATTCGCCGACGATCATGATCGCGGAAAAGGCAGCCGAGATGATCCTGGCTGCAGATCGCTAG

Upstream 100 bases:

>100_bases
CCGCTCTCACTTACTGGAGTCATGTTTTGGCCGACCCGGCCTAAAAACATCGTGATCGAGCATCGGTCCGAACCATCGGG
ACCTCGACAAAGAGAAAAGC

Downstream 100 bases:

>100_bases
AGCAACTAGGAAAAGTGCGTAGCCGTTTTCCGCTGCCCGTTCAGTCTATTCCGCCGCCTCGGCCTCCGGGGCCGGCACGT
AGTTGAGGACCGGCGACAGC

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MDTFDYIIVGAGSAGCVLANRLSENPDRRVLLLEAGGSDNYHWIHIPVGYLYCINNPRTDWCFTTAAEEGLNGRSLGYPR
GKVLGGCSSINGMIYMRGQARDYDLWRQLGCAGWSWNDVLPLFRKCEDHHRGADEMHGAGGEWRVEKARVRWAVLDAFQK
AATEAGIPETDDFNRGTNEGSGYFDVNQRSGIRWNTAKAFLRPAMRRRNLTILTKAHVRRLVLNDRRVSGVEFQHDGVTK
SVLARREVVLSAGAIGSPHILELSGIGRPDVLRENGIEVRHELPAVGENLQDHLQLRLAYKVTGVPTLNEKATSLFGKAA
IGLEYLVRRSGPMAMAPSQLGIFTRSGPEKETPDLQYHVQPVTLEKFGEPVHPFPAITASVCNLRPESRGSVHLKSPDFA
AAPNIRPRYLSAEADREVAVKAIRLTRRIVSQPAFARYKPVEFKPGPSYETDEDLKRAAGEIGTTIFHPVGTCRMGGDQA
SVVDPELRLRGLAGLRIADASIMPTITSGNTNSPTIMIAEKAAEMILAADR

Sequences:

>Translated_531_residues
MDTFDYIIVGAGSAGCVLANRLSENPDRRVLLLEAGGSDNYHWIHIPVGYLYCINNPRTDWCFTTAAEEGLNGRSLGYPR
GKVLGGCSSINGMIYMRGQARDYDLWRQLGCAGWSWNDVLPLFRKCEDHHRGADEMHGAGGEWRVEKARVRWAVLDAFQK
AATEAGIPETDDFNRGTNEGSGYFDVNQRSGIRWNTAKAFLRPAMRRRNLTILTKAHVRRLVLNDRRVSGVEFQHDGVTK
SVLARREVVLSAGAIGSPHILELSGIGRPDVLRENGIEVRHELPAVGENLQDHLQLRLAYKVTGVPTLNEKATSLFGKAA
IGLEYLVRRSGPMAMAPSQLGIFTRSGPEKETPDLQYHVQPVTLEKFGEPVHPFPAITASVCNLRPESRGSVHLKSPDFA
AAPNIRPRYLSAEADREVAVKAIRLTRRIVSQPAFARYKPVEFKPGPSYETDEDLKRAAGEIGTTIFHPVGTCRMGGDQA
SVVDPELRLRGLAGLRIADASIMPTITSGNTNSPTIMIAEKAAEMILAADR
>Mature_531_residues
MDTFDYIIVGAGSAGCVLANRLSENPDRRVLLLEAGGSDNYHWIHIPVGYLYCINNPRTDWCFTTAAEEGLNGRSLGYPR
GKVLGGCSSINGMIYMRGQARDYDLWRQLGCAGWSWNDVLPLFRKCEDHHRGADEMHGAGGEWRVEKARVRWAVLDAFQK
AATEAGIPETDDFNRGTNEGSGYFDVNQRSGIRWNTAKAFLRPAMRRRNLTILTKAHVRRLVLNDRRVSGVEFQHDGVTK
SVLARREVVLSAGAIGSPHILELSGIGRPDVLRENGIEVRHELPAVGENLQDHLQLRLAYKVTGVPTLNEKATSLFGKAA
IGLEYLVRRSGPMAMAPSQLGIFTRSGPEKETPDLQYHVQPVTLEKFGEPVHPFPAITASVCNLRPESRGSVHLKSPDFA
AAPNIRPRYLSAEADREVAVKAIRLTRRIVSQPAFARYKPVEFKPGPSYETDEDLKRAAGEIGTTIFHPVGTCRMGGDQA
SVVDPELRLRGLAGLRIADASIMPTITSGNTNSPTIMIAEKAAEMILAADR

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=534, Percent_Identity=41.5730337078652, Blast_Score=400, Evalue=1e-111,
Organism=Escherichia coli, GI1786503, Length=531, Percent_Identity=42.1845574387947, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17532301, Length=542, Percent_Identity=35.4243542435424, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24642055, Length=576, Percent_Identity=37.3263888888889, Blast_Score=321, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24642042, Length=567, Percent_Identity=35.626102292769, Blast_Score=308, Evalue=8e-84,
Organism=Drosophila melanogaster, GI24642048, Length=573, Percent_Identity=36.1256544502618, Blast_Score=305, Evalue=6e-83,
Organism=Drosophila melanogaster, GI45549471, Length=565, Percent_Identity=35.0442477876106, Blast_Score=299, Evalue=4e-81,
Organism=Drosophila melanogaster, GI45551458, Length=565, Percent_Identity=35.0442477876106, Blast_Score=298, Evalue=6e-81,
Organism=Drosophila melanogaster, GI17137792, Length=556, Percent_Identity=35.6115107913669, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24642059, Length=569, Percent_Identity=34.7978910369069, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI18859995, Length=564, Percent_Identity=35.6382978723404, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24642035, Length=574, Percent_Identity=32.2299651567944, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24650267, Length=571, Percent_Identity=34.8511383537653, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24642039, Length=567, Percent_Identity=34.2151675485009, Blast_Score=262, Evalue=4e-70,
Organism=Drosophila melanogaster, GI18859993, Length=566, Percent_Identity=33.2155477031802, Blast_Score=261, Evalue=6e-70,
Organism=Drosophila melanogaster, GI24642037, Length=571, Percent_Identity=33.4500875656743, Blast_Score=255, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24642051, Length=564, Percent_Identity=32.8014184397163, Blast_Score=254, Evalue=8e-68,
Organism=Drosophila melanogaster, GI24645930, Length=582, Percent_Identity=30.4123711340206, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24642057, Length=582, Percent_Identity=28.1786941580756, Blast_Score=155, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58484; Mature: 58484

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00624 GMC_OXRED_2 ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTFDYIIVGAGSAGCVLANRLSENPDRRVLLLEAGGSDNYHWIHIPVGYLYCINNPRTD
CCCEEEEEEECCCCCHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECEEEEEECCCCCC
WCFTTAAEEGLNGRSLGYPRGKVLGGCSSINGMIYMRGQARDYDLWRQLGCAGWSWNDVL
EEEEEHHHCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHH
PLFRKCEDHHRGADEMHGAGGEWRVEKARVRWAVLDAFQKAATEAGIPETDDFNRGTNEG
HHHHHHHHHCCCHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SGYFDVNQRSGIRWNTAKAFLRPAMRRRNLTILTKAHVRRLVLNDRRVSGVEFQHDGVTK
CCEEEECCCCCCEEHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCCEEEECCCCHH
SVLARREVVLSAGAIGSPHILELSGIGRPDVLRENGIEVRHELPAVGENLQDHLQLRLAY
HHHHHHHHHEECCCCCCCCEEEECCCCCCCHHHCCCCCEEHHCCHHCCCHHHHEEEEEEE
KVTGVPTLNEKATSLFGKAAIGLEYLVRRSGPMAMAPSQLGIFTRSGPEKETPDLQYHVQ
EEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEECCCCCCCCCCCEEEEE
PVTLEKFGEPVHPFPAITASVCNLRPESRGSVHLKSPDFAAAPNIRPRYLSAEADREVAV
EEEHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCHHHHH
KAIRLTRRIVSQPAFARYKPVEFKPGPSYETDEDLKRAAGEIGTTIFHPVGTCRMGGDQA
HHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCC
SVVDPELRLRGLAGLRIADASIMPTITSGNTNSPTIMIAEKAAEMILAADR
CEECHHHHHHCCCCEEEECCCCCEEEECCCCCCCEEEEECHHHHHHEECCC
>Mature Secondary Structure
MDTFDYIIVGAGSAGCVLANRLSENPDRRVLLLEAGGSDNYHWIHIPVGYLYCINNPRTD
CCCEEEEEEECCCCCHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECEEEEEECCCCCC
WCFTTAAEEGLNGRSLGYPRGKVLGGCSSINGMIYMRGQARDYDLWRQLGCAGWSWNDVL
EEEEEHHHCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHH
PLFRKCEDHHRGADEMHGAGGEWRVEKARVRWAVLDAFQKAATEAGIPETDDFNRGTNEG
HHHHHHHHHCCCHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SGYFDVNQRSGIRWNTAKAFLRPAMRRRNLTILTKAHVRRLVLNDRRVSGVEFQHDGVTK
CCEEEECCCCCCEEHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCCEEEECCCCHH
SVLARREVVLSAGAIGSPHILELSGIGRPDVLRENGIEVRHELPAVGENLQDHLQLRLAY
HHHHHHHHHEECCCCCCCCEEEECCCCCCCHHHCCCCCEEHHCCHHCCCHHHHEEEEEEE
KVTGVPTLNEKATSLFGKAAIGLEYLVRRSGPMAMAPSQLGIFTRSGPEKETPDLQYHVQ
EEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEECCCCCCCCCCCEEEEE
PVTLEKFGEPVHPFPAITASVCNLRPESRGSVHLKSPDFAAAPNIRPRYLSAEADREVAV
EEEHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCHHHHH
KAIRLTRRIVSQPAFARYKPVEFKPGPSYETDEDLKRAAGEIGTTIFHPVGTCRMGGDQA
HHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCC
SVVDPELRLRGLAGLRIADASIMPTITSGNTNSPTIMIAEKAAEMILAADR
CEECHHHHHHCCCCEEEECCCCCEEEECCCCCCCEEEEECHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]