Click here to switch to the map view.

The map label for this gene is livM [H]

Identifier: 150398066

GI number: 150398066

Start: 3000402

End: 3001394

Strand: Direct

Name: livM [H]

Synonym: Smed_2868

Alternate gene names: 150398066

Gene position: 3000402-3001394 (Clockwise)

Preceding gene: 150398065

Following gene: 150398067

Centisome position: 79.34

GC content: 60.02

Gene sequence:

>993_bases
ATGACATTGACACTCGATCTCGCCAAGCGGAAGGAGCGTTCGCCGTTCATCGTTCAGACGGTGTTCCTCGGCGTGGGCCT
TGCCTGCCTCCTGCTTGCTCCCTTGTTCTTCTATCCCGTTTTCCTGATGAAGCTTCTGTGCTTCGCGCTGTTCGCCTGCG
CTTTCAATCTGCTGCTCGGCTATACGGGGCTACTCTCGTTCGGCCACGCTGCCTTTTTCGGCGGGGCGGCTTATTTCACC
GCCCATGCCGTGAAGGAATGGGGCGTCCCACCCGAACTCGGAATCCTCGTCGGCGTCTTCGGCGCGGCGCTGCTCGGCGC
CGTCATCGGCTTCTTCGCCATTCGCCGGCAGGGCATCTACTTCGCCATGATCACACTGGCACTGGCGCAGATGTTCTATT
TCTTCTGCCTCCAGTCCGGGTTCACTCGAGGCGAGGACGGAATTCAGTCGGTGCCTCGCGGCCATCTCCTCGGAGTCATC
GATCTCTTGCAGCCGACGAACATGTATTATTTCGTGCTCACCGTCTTCGTCATCGGCATCGCAATCATCTGGCGGATCAT
CAATTCGCCCTTCGGGATGGTCTTGAAGTCGATCCGCGAAAACGAGACGCGGGCGGTGTCGCTCGGCTATTCGGTAAGAA
ACTACAAGCTCGCAGCCTTCGTCATGTCGGCGGCACTTACCGGTCTCGCCGGCGGTCTGAAGGCGCTCGTTTTCCAGTTC
GCGACGCTGACCGACGTCAGCTGGCAGATGTCGGGCGAGGTCATCCTCATGACGCTTCTCGGCGGCATCGGAACGCTGAT
CGGACCGATCTTCGGGGCGGGCCTCGTCGTCACGCTGCAGAACTACCTGGCGACGTCGGAATTCCCGGTCACCATCATCA
CCGGAGTCGTCTTCATGGTTTGCGTGCTTCTGTTCCGCCGCGGCATCGTTGGTGAGTTTTACAACTCCCGGCTGGGCCGC
AAGCTCGGCTTCGAGCACAGGCACAAGCACTGA

Upstream 100 bases:

>100_bases
AAGGTCTTCTACCCGGAAGCCTCCAACATCGTGATCTTCGTGATCATGGCCATCGTTCTCCTGCTGAGGCCCGCGGGTCT
CTTCGGCCGGGATGCCTGAT

Downstream 100 bases:

>100_bases
CCTCGAGCGAGATGAGGAAAAATGTGCGGTTTTCCGCCCGTATTCCGCTCTCACTTACTGGAGTCATGTTTTGGCCGACC
CGGCCTAAAAACATCGTGAT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTLTLDLAKRKERSPFIVQTVFLGVGLACLLLAPLFFYPVFLMKLLCFALFACAFNLLLGYTGLLSFGHAAFFGGAAYFT
AHAVKEWGVPPELGILVGVFGAALLGAVIGFFAIRRQGIYFAMITLALAQMFYFFCLQSGFTRGEDGIQSVPRGHLLGVI
DLLQPTNMYYFVLTVFVIGIAIIWRIINSPFGMVLKSIRENETRAVSLGYSVRNYKLAAFVMSAALTGLAGGLKALVFQF
ATLTDVSWQMSGEVILMTLLGGIGTLIGPIFGAGLVVTLQNYLATSEFPVTIITGVVFMVCVLLFRRGIVGEFYNSRLGR
KLGFEHRHKH

Sequences:

>Translated_330_residues
MTLTLDLAKRKERSPFIVQTVFLGVGLACLLLAPLFFYPVFLMKLLCFALFACAFNLLLGYTGLLSFGHAAFFGGAAYFT
AHAVKEWGVPPELGILVGVFGAALLGAVIGFFAIRRQGIYFAMITLALAQMFYFFCLQSGFTRGEDGIQSVPRGHLLGVI
DLLQPTNMYYFVLTVFVIGIAIIWRIINSPFGMVLKSIRENETRAVSLGYSVRNYKLAAFVMSAALTGLAGGLKALVFQF
ATLTDVSWQMSGEVILMTLLGGIGTLIGPIFGAGLVVTLQNYLATSEFPVTIITGVVFMVCVLLFRRGIVGEFYNSRLGR
KLGFEHRHKH
>Mature_329_residues
TLTLDLAKRKERSPFIVQTVFLGVGLACLLLAPLFFYPVFLMKLLCFALFACAFNLLLGYTGLLSFGHAAFFGGAAYFTA
HAVKEWGVPPELGILVGVFGAALLGAVIGFFAIRRQGIYFAMITLALAQMFYFFCLQSGFTRGEDGIQSVPRGHLLGVID
LLQPTNMYYFVLTVFVIGIAIIWRIINSPFGMVLKSIRENETRAVSLGYSVRNYKLAAFVMSAALTGLAGGLKALVFQFA
TLTDVSWQMSGEVILMTLLGGIGTLIGPIFGAGLVVTLQNYLATSEFPVTIITGVVFMVCVLLFRRGIVGEFYNSRLGRK
LGFEHRHKH

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=290, Percent_Identity=26.551724137931, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36109; Mature: 35978

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTLDLAKRKERSPFIVQTVFLGVGLACLLLAPLFFYPVFLMKLLCFALFACAFNLLLG
CEEEEECHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTGLLSFGHAAFFGGAAYFTAHAVKEWGVPPELGILVGVFGAALLGAVIGFFAIRRQGIY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
FAMITLALAQMFYFFCLQSGFTRGEDGIQSVPRGHLLGVIDLLQPTNMYYFVLTVFVIGI
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
AIIWRIINSPFGMVLKSIRENETRAVSLGYSVRNYKLAAFVMSAALTGLAGGLKALVFQF
HHHHHHHCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLTDVSWQMSGEVILMTLLGGIGTLIGPIFGAGLVVTLQNYLATSEFPVTIITGVVFMV
HHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
CVLLFRRGIVGEFYNSRLGRKLGFEHRHKH
HHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TLTLDLAKRKERSPFIVQTVFLGVGLACLLLAPLFFYPVFLMKLLCFALFACAFNLLLG
EEEEECHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HHHHHHCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]