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The map label for this gene is braD [H]

Identifier: 150398065

GI number: 150398065

Start: 2999513

End: 3000400

Strand: Direct

Name: braD [H]

Synonym: Smed_2867

Alternate gene names: 150398065

Gene position: 2999513-3000400 (Clockwise)

Preceding gene: 150398064

Following gene: 150398066

Centisome position: 79.31

GC content: 61.04

Gene sequence:

>888_bases
ATGACGATGATTTTCGGAATCCCACTTCAGGCTTTCCTAGGGCAGCTCCTGATCGGGCTCATCAACGGCTCCTTCTACGC
ACTCCTCAGTCTCGGCCTCGCCATCATCTTCGGGCTCTTGCGTGTCATCAATTTCGCCCATGGCGCTCAATACATGCTCG
GCGCCTTCATCGCCTGGCTGCTGCTCTCCTATCTCGGAATCGGCTATTGGCCGGCACTGGTTCTTGCGCCGCTCCTCGTC
GGTCTCATCGGCGCCGCGATCGAACGCACGATGCTGAGGCAGCTCTATTCTCTCGACCCACTTTACGGTCTCCTTTTCAC
CTTTGGCCTGGCCCTGACCGTAGAAGGCACCTTCCGCTATATTTACGGCGCATCCGGCCAGCCCTATGCGACACCGGCGC
TGCTGGCCGGCGGAGCCAATCTCGGCTTCATGTTCCTTCCCATCTATCGCGGCTGGGTCATCGTCTTCTCGCTGATCGTC
TGCCTCGGCACCTGGCTCGTCATTGAGAAGACCAAGCTCGGCTCCTATCTGCGCGCCGCAACGGAGAACCCGGTTCTGGT
GCAATCCTTCGGCATCAACGTGCCCTTCCTGCTGACACTGACCTACGGGCTCGGCGCAGCGCTCGCCGCGCTTGCCGGAG
TCCTTGCCGCGCCGATCTATCAGGTCTCGCCGCTGATGGGCTCGAACATCATCATCGTCGTCTTCGCGGTGGTCGTCGTC
GGCGGCATGGGCTCGATCATGGGCGCAATCGTCACAGGCTACCTGCTCGGGGTCGCCGAAGGACTGACCAAGGTCTTCTA
CCCGGAAGCCTCCAACATCGTGATCTTCGTGATCATGGCCATCGTTCTCCTGCTGAGGCCCGCGGGTCTCTTCGGCCGGG
ATGCCTGA

Upstream 100 bases:

>100_bases
CTTCTACCTGATGGATCATGGGCGTGTCGCCGGCGAGTTCCCGGTCTCGGAGCTTTCCGGGCGCATGGAAATGCTGCATG
ATGTGCTCGGGGTGTAAGCC

Downstream 100 bases:

>100_bases
TATGACATTGACACTCGATCTCGCCAAGCGGAAGGAGCGTTCGCCGTTCATCGTTCAGACGGTGTTCCTCGGCGTGGGCC
TTGCCTGCCTCCTGCTTGCT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTMIFGIPLQAFLGQLLIGLINGSFYALLSLGLAIIFGLLRVINFAHGAQYMLGAFIAWLLLSYLGIGYWPALVLAPLLV
GLIGAAIERTMLRQLYSLDPLYGLLFTFGLALTVEGTFRYIYGASGQPYATPALLAGGANLGFMFLPIYRGWVIVFSLIV
CLGTWLVIEKTKLGSYLRAATENPVLVQSFGINVPFLLTLTYGLGAALAALAGVLAAPIYQVSPLMGSNIIIVVFAVVVV
GGMGSIMGAIVTGYLLGVAEGLTKVFYPEASNIVIFVIMAIVLLLRPAGLFGRDA

Sequences:

>Translated_295_residues
MTMIFGIPLQAFLGQLLIGLINGSFYALLSLGLAIIFGLLRVINFAHGAQYMLGAFIAWLLLSYLGIGYWPALVLAPLLV
GLIGAAIERTMLRQLYSLDPLYGLLFTFGLALTVEGTFRYIYGASGQPYATPALLAGGANLGFMFLPIYRGWVIVFSLIV
CLGTWLVIEKTKLGSYLRAATENPVLVQSFGINVPFLLTLTYGLGAALAALAGVLAAPIYQVSPLMGSNIIIVVFAVVVV
GGMGSIMGAIVTGYLLGVAEGLTKVFYPEASNIVIFVIMAIVLLLRPAGLFGRDA
>Mature_294_residues
TMIFGIPLQAFLGQLLIGLINGSFYALLSLGLAIIFGLLRVINFAHGAQYMLGAFIAWLLLSYLGIGYWPALVLAPLLVG
LIGAAIERTMLRQLYSLDPLYGLLFTFGLALTVEGTFRYIYGASGQPYATPALLAGGANLGFMFLPIYRGWVIVFSLIVC
LGTWLVIEKTKLGSYLRAATENPVLVQSFGINVPFLLTLTYGLGAALAALAGVLAAPIYQVSPLMGSNIIIVVFAVVVVG
GMGSIMGAIVTGYLLGVAEGLTKVFYPEASNIVIFVIMAIVLLLRPAGLFGRDA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=304, Percent_Identity=31.5789473684211, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31338; Mature: 31207

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMIFGIPLQAFLGQLLIGLINGSFYALLSLGLAIIFGLLRVINFAHGAQYMLGAFIAWL
CCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSYLGIGYWPALVLAPLLVGLIGAAIERTMLRQLYSLDPLYGLLFTFGLALTVEGTFRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEECCEEE
IYGASGQPYATPALLAGGANLGFMFLPIYRGWVIVFSLIVCLGTWLVIEKTKLGSYLRAA
EECCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TENPVLVQSFGINVPFLLTLTYGLGAALAALAGVLAAPIYQVSPLMGSNIIIVVFAVVVV
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
GGMGSIMGAIVTGYLLGVAEGLTKVFYPEASNIVIFVIMAIVLLLRPAGLFGRDA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TMIFGIPLQAFLGQLLIGLINGSFYALLSLGLAIIFGLLRVINFAHGAQYMLGAFIAWL
CEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSYLGIGYWPALVLAPLLVGLIGAAIERTMLRQLYSLDPLYGLLFTFGLALTVEGTFRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEECCEEE
IYGASGQPYATPALLAGGANLGFMFLPIYRGWVIVFSLIVCLGTWLVIEKTKLGSYLRAA
EECCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TENPVLVQSFGINVPFLLTLTYGLGAALAALAGVLAAPIYQVSPLMGSNIIIVVFAVVVV
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
GGMGSIMGAIVTGYLLGVAEGLTKVFYPEASNIVIFVIMAIVLLLRPAGLFGRDA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]