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The map label for this gene is puuB [H]

Identifier: 150398047

GI number: 150398047

Start: 2975619

End: 2976899

Strand: Reverse

Name: puuB [H]

Synonym: Smed_2849

Alternate gene names: 150398047

Gene position: 2976899-2975619 (Counterclockwise)

Preceding gene: 150398048

Following gene: 150398046

Centisome position: 78.71

GC content: 62.53

Gene sequence:

>1281_bases
ATGTCTTATCCCAACACGTATTACAAACAGACGATTGCCGACACGAAGGTCAGGCCAGCCCTTTCCGGGGCGGTCGAATG
CGATACCGTGATCATCGGTGGCGGCCTTGCCGGCCTGACGACGGCCTTGCAACTGACGCGCGCCGGGCAGACCGTCGTCG
TCCTCGAGGCCGAAAGCGTGGGCTTCGGAGCCTCCGGGCGCAATGGCGGCTTCGTCAGTCCGGGCTTTGCCACCGGCAGC
GACAATATCGCCCGCATGGCCGGGACAGAAGCGGCGCGACAGATACACCGGCTTTCGATCGAGGGCGTCGAATTCGTTCG
CGAGACGATCGAGGCGCTGAAGATCGAGGGCGCAAGGCCGCAGACGGGAATCACCAGCGTATTGCGTTACGACGATGGTG
AGGGGCTGAAAGCCCATGCTGACGAGATGAGACGCGTCTACGGTTACGAACTCGACTATCTCTCCACCGACGAGGTTCGC
TCGGTGCTGAAGTCAAAGCGTTATTTCCACGCCTTGCGCGACCCCAAGGCCTTCCACATGCATCCGCTGAATTATCTTCG
CGGGCTCGCACAGGAGATCGAACGTCTCGGTGGCCGGATCTATGAAGGCTCGGCGGTGACGGGGAGTGCGTTGGACAGCC
CCGAAAAGACCGTGTCGACCTCGGGTGGATGTGCTCGAGCGCGCAATGTCGTCTTCACCACCGGCGGCTATACCGGTTCC
CTTGACGGAAGGCTGAAGCGTTCGTTCCTGCCGATTGCAACCTATGTCATGCTGAGCGAGGAAGCGCCGGAACTTATCCG
CACCGCCATCCTCACGACGGATGCGATCGGCGACAACCGTCGTGCCGGGGATTACTACCGCCTGGTCGAAGGCGGAAAGC
GTCTGCTTTGGGGCGGGCGCATCACCACACGCGCGGCTTCGCCTGCAACCCTGGCCGGAGAGCTGCGTCGCGAAATGGTG
GGGACCTATCCTCAGCTCAAGGACCTGAAAACCGAACTCGCCTGGTCGGGACTGATGTCATACGCGCGCCATCTCATGCC
ACAGATCGGCGAGATGCAGGCGGGCGTGTGGCATTGCACCGCCTTCGGCGGCCACGGGCTGAACACCACTGCGGTCGGCG
GAAAGCTGGTTGCCGAAGGCATTCTTCGCCAGTCCGACCGGTACAAGCTGTTCAAGCCGTTCGGGCTCGTCTGGGCGGGC
GGATATGCCGGACTGGCGGTAGCCCAGCTCACCTATTGGAAACTGCAAGCTCAAGACTGGTGGCGCGAGCGCGCGGCCTG
A

Upstream 100 bases:

>100_bases
CAAGTGGTACGGCAAGGATTACAGCGCCGACTGATCCCGATCAATGCGCGGGCGGCCTGCCGCCCGCCGCCTTACCACCG
CGGTTGACGGGCGATAACGC

Downstream 100 bases:

>100_bases
ATGCCGGGGAGAACCAAATGATCGGTCGTATGATTCTTACATATCCGGATGCAGCGCGGCGTACCGGGACATTTCTCCTG
CTCGCGCTGATAACCCTGAT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSYPNTYYKQTIADTKVRPALSGAVECDTVIIGGGLAGLTTALQLTRAGQTVVVLEAESVGFGASGRNGGFVSPGFATGS
DNIARMAGTEAARQIHRLSIEGVEFVRETIEALKIEGARPQTGITSVLRYDDGEGLKAHADEMRRVYGYELDYLSTDEVR
SVLKSKRYFHALRDPKAFHMHPLNYLRGLAQEIERLGGRIYEGSAVTGSALDSPEKTVSTSGGCARARNVVFTTGGYTGS
LDGRLKRSFLPIATYVMLSEEAPELIRTAILTTDAIGDNRRAGDYYRLVEGGKRLLWGGRITTRAASPATLAGELRREMV
GTYPQLKDLKTELAWSGLMSYARHLMPQIGEMQAGVWHCTAFGGHGLNTTAVGGKLVAEGILRQSDRYKLFKPFGLVWAG
GYAGLAVAQLTYWKLQAQDWWRERAA

Sequences:

>Translated_426_residues
MSYPNTYYKQTIADTKVRPALSGAVECDTVIIGGGLAGLTTALQLTRAGQTVVVLEAESVGFGASGRNGGFVSPGFATGS
DNIARMAGTEAARQIHRLSIEGVEFVRETIEALKIEGARPQTGITSVLRYDDGEGLKAHADEMRRVYGYELDYLSTDEVR
SVLKSKRYFHALRDPKAFHMHPLNYLRGLAQEIERLGGRIYEGSAVTGSALDSPEKTVSTSGGCARARNVVFTTGGYTGS
LDGRLKRSFLPIATYVMLSEEAPELIRTAILTTDAIGDNRRAGDYYRLVEGGKRLLWGGRITTRAASPATLAGELRREMV
GTYPQLKDLKTELAWSGLMSYARHLMPQIGEMQAGVWHCTAFGGHGLNTTAVGGKLVAEGILRQSDRYKLFKPFGLVWAG
GYAGLAVAQLTYWKLQAQDWWRERAA
>Mature_425_residues
SYPNTYYKQTIADTKVRPALSGAVECDTVIIGGGLAGLTTALQLTRAGQTVVVLEAESVGFGASGRNGGFVSPGFATGSD
NIARMAGTEAARQIHRLSIEGVEFVRETIEALKIEGARPQTGITSVLRYDDGEGLKAHADEMRRVYGYELDYLSTDEVRS
VLKSKRYFHALRDPKAFHMHPLNYLRGLAQEIERLGGRIYEGSAVTGSALDSPEKTVSTSGGCARARNVVFTTGGYTGSL
DGRLKRSFLPIATYVMLSEEAPELIRTAILTTDAIGDNRRAGDYYRLVEGGKRLLWGGRITTRAASPATLAGELRREMVG
TYPQLKDLKTELAWSGLMSYARHLMPQIGEMQAGVWHCTAFGGHGLNTTAVGGKLVAEGILRQSDRYKLFKPFGLVWAGG
YAGLAVAQLTYWKLQAQDWWRERAA

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=391, Percent_Identity=31.7135549872123, Blast_Score=194, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46371; Mature: 46240

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYPNTYYKQTIADTKVRPALSGAVECDTVIIGGGLAGLTTALQLTRAGQTVVVLEAESV
CCCCCHHHHHHHHHCCCCHHHCCCCEECEEEECCCHHHHHHHHHHHCCCCEEEEEEECCC
GFGASGRNGGFVSPGFATGSDNIARMAGTEAARQIHRLSIEGVEFVRETIEALKIEGARP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QTGITSVLRYDDGEGLKAHADEMRRVYGYELDYLSTDEVRSVLKSKRYFHALRDPKAFHM
CCCHHHHHEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
HPLNYLRGLAQEIERLGGRIYEGSAVTGSALDSPEKTVSTSGGCARARNVVFTTGGYTGS
CHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHCCCCCHHHHCCEEEEECCCCCC
LDGRLKRSFLPIATYVMLSEEAPELIRTAILTTDAIGDNRRAGDYYRLVEGGKRLLWGGR
CCCHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEECCC
ITTRAASPATLAGELRREMVGTYPQLKDLKTELAWSGLMSYARHLMPQIGEMQAGVWHCT
EEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
AFGGHGLNTTAVGGKLVAEGILRQSDRYKLFKPFGLVWAGGYAGLAVAQLTYWKLQAQDW
EECCCCCCCHHCCHHHHHHHHHHCCCCCEEECCCCEEEECCHHHHHHHHHHHHEEHHHHH
WRERAA
HHHHCC
>Mature Secondary Structure 
SYPNTYYKQTIADTKVRPALSGAVECDTVIIGGGLAGLTTALQLTRAGQTVVVLEAESV
CCCCHHHHHHHHHCCCCHHHCCCCEECEEEECCCHHHHHHHHHHHCCCCEEEEEEECCC
GFGASGRNGGFVSPGFATGSDNIARMAGTEAARQIHRLSIEGVEFVRETIEALKIEGARP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QTGITSVLRYDDGEGLKAHADEMRRVYGYELDYLSTDEVRSVLKSKRYFHALRDPKAFHM
CCCHHHHHEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
HPLNYLRGLAQEIERLGGRIYEGSAVTGSALDSPEKTVSTSGGCARARNVVFTTGGYTGS
CHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHCCCCCHHHHCCEEEEECCCCCC
LDGRLKRSFLPIATYVMLSEEAPELIRTAILTTDAIGDNRRAGDYYRLVEGGKRLLWGGR
CCCHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEECCC
ITTRAASPATLAGELRREMVGTYPQLKDLKTELAWSGLMSYARHLMPQIGEMQAGVWHCT
EEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
AFGGHGLNTTAVGGKLVAEGILRQSDRYKLFKPFGLVWAGGYAGLAVAQLTYWKLQAQDW
EECCCCCCCHHCCHHHHHHHHHHCCCCCEEECCCCEEEECCHHHHHHHHHHHHEEHHHHH
WRERAA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]