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The map label for this gene is fliY [H]

Identifier: 150398048

GI number: 150398048

Start: 2976966

End: 2977793

Strand: Reverse

Name: fliY [H]

Synonym: Smed_2850

Alternate gene names: 150398048

Gene position: 2977793-2976966 (Counterclockwise)

Preceding gene: 150398049

Following gene: 150398047

Centisome position: 78.74

GC content: 60.75

Gene sequence:

>828_bases
GTGAAGGGATTATTTGCAAAGCTCGCCATCGGCGTTGCCGCACTGGCGTTTGTCACCGCCGCACAGGCAGGTGAGACACT
CGACCGGGTGATGGGCAAAAAGGCCATGGTGGTTGCCACCAACAGCGGCTGGCCGCCCCAGAGTTTTCTCGACGACAGCA
ACGAACTGGTCGGCTTCGACATCGACGTTTCCAAGGAGATCGCCAAGCGCCTCGGCGTCGAAGTGAGCTTTGAGACGCCG
GACTGGGCCACATTGACCGGCGGCCGTTGGCAGGGGCGCTACGATCTGGGCGTCGGTTCCGTCACCCCGACCAAGGCGCG
TGCTCAGGTCATCGATTTCGTAGGCATCTATTACTTCAGCCCCTATGTCTACGTATTGCACAAGGATAGCCAGGCGAAAT
CGGTCGGCGACCTGAACGGCAAGAAGATCGGCGTCGAAACGGCGACCACGTCGGAAGACTTCATTCGCCGCCGTCTGGAG
ATCGACGCACCCGGCCTGCCGCCGATCGAATACAAGATCGAGCCTGGCGAGGTCCGCACCTACGCCGATTCGATGCTACC
CTTCGACGATCTGCGCATTGGCGACGGCACGCGCCTTGACGCGGTTATCGCACCGGAGCAGACAGCGCTCAACGCAATCA
AGAACGGCTATCCGCTGCGTGTCCTCGAGGGCGAGTATGCGTTCAGGGAGCCGTTGGTGGTGATCGCTGAAAAGGTCGAT
CCGGACTGGACGAAGAAAGTCGGTGCCGTCATCGAAGAGATGAAGACCGACGGAACGCTCGCGGCGCTGACCACCAAGTG
GTACGGCAAGGATTACAGCGCCGACTGA

Upstream 100 bases:

>100_bases
ATGTTCCGCGAACGTGCAGCAACGCGAAGTGCTACAGCGATCTTTGCGTGTCTTGATAAGGCGCAACGCTGCAGACTGAC
CGATCGAAGAGGAGGATTGC

Downstream 100 bases:

>100_bases
TCCCGATCAATGCGCGGGCGGCCTGCCGCCCGCCGCCTTACCACCGCGGTTGACGGGCGATAACGCATGTCTTATCCCAA
CACGTATTACAAACAGACGA

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKGLFAKLAIGVAALAFVTAAQAGETLDRVMGKKAMVVATNSGWPPQSFLDDSNELVGFDIDVSKEIAKRLGVEVSFETP
DWATLTGGRWQGRYDLGVGSVTPTKARAQVIDFVGIYYFSPYVYVLHKDSQAKSVGDLNGKKIGVETATTSEDFIRRRLE
IDAPGLPPIEYKIEPGEVRTYADSMLPFDDLRIGDGTRLDAVIAPEQTALNAIKNGYPLRVLEGEYAFREPLVVIAEKVD
PDWTKKVGAVIEEMKTDGTLAALTTKWYGKDYSAD

Sequences:

>Translated_275_residues
MKGLFAKLAIGVAALAFVTAAQAGETLDRVMGKKAMVVATNSGWPPQSFLDDSNELVGFDIDVSKEIAKRLGVEVSFETP
DWATLTGGRWQGRYDLGVGSVTPTKARAQVIDFVGIYYFSPYVYVLHKDSQAKSVGDLNGKKIGVETATTSEDFIRRRLE
IDAPGLPPIEYKIEPGEVRTYADSMLPFDDLRIGDGTRLDAVIAPEQTALNAIKNGYPLRVLEGEYAFREPLVVIAEKVD
PDWTKKVGAVIEEMKTDGTLAALTTKWYGKDYSAD
>Mature_275_residues
MKGLFAKLAIGVAALAFVTAAQAGETLDRVMGKKAMVVATNSGWPPQSFLDDSNELVGFDIDVSKEIAKRLGVEVSFETP
DWATLTGGRWQGRYDLGVGSVTPTKARAQVIDFVGIYYFSPYVYVLHKDSQAKSVGDLNGKKIGVETATTSEDFIRRRLE
IDAPGLPPIEYKIEPGEVRTYADSMLPFDDLRIGDGTRLDAVIAPEQTALNAIKNGYPLRVLEGEYAFREPLVVIAEKVD
PDWTKKVGAVIEEMKTDGTLAALTTKWYGKDYSAD

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=271, Percent_Identity=29.8892988929889, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787085, Length=262, Percent_Identity=23.6641221374046, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787088, Length=264, Percent_Identity=24.2424242424242, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787031, Length=268, Percent_Identity=26.1194029850746, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788648, Length=247, Percent_Identity=26.7206477732794, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30077; Mature: 30077

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLFAKLAIGVAALAFVTAAQAGETLDRVMGKKAMVVATNSGWPPQSFLDDSNELVGFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCCCCEEEEE
IDVSKEIAKRLGVEVSFETPDWATLTGGRWQGRYDLGVGSVTPTKARAQVIDFVGIYYFS
CCHHHHHHHHCCCEEEECCCCCEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHC
PYVYVLHKDSQAKSVGDLNGKKIGVETATTSEDFIRRRLEIDAPGLPPIEYKIEPGEVRT
CEEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHH
YADSMLPFDDLRIGDGTRLDAVIAPEQTALNAIKNGYPLRVLEGEYAFREPLVVIAEKVD
HHHHCCCHHHEECCCCCEEEEEECCCHHHHHHHHCCCCEEEECCCCHHHCCEEEEEECCC
PDWTKKVGAVIEEMKTDGTLAALTTKWYGKDYSAD
CHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCC
>Mature Secondary Structure
MKGLFAKLAIGVAALAFVTAAQAGETLDRVMGKKAMVVATNSGWPPQSFLDDSNELVGFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCCCCEEEEE
IDVSKEIAKRLGVEVSFETPDWATLTGGRWQGRYDLGVGSVTPTKARAQVIDFVGIYYFS
CCHHHHHHHHCCCEEEECCCCCEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHC
PYVYVLHKDSQAKSVGDLNGKKIGVETATTSEDFIRRRLEIDAPGLPPIEYKIEPGEVRT
CEEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHH
YADSMLPFDDLRIGDGTRLDAVIAPEQTALNAIKNGYPLRVLEGEYAFREPLVVIAEKVD
HHHHCCCHHHEECCCCCEEEEEECCCHHHHHHHHCCCCEEEECCCCHHHCCEEEEEECCC
PDWTKKVGAVIEEMKTDGTLAALTTKWYGKDYSAD
CHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]