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The map label for this gene is lpdV [H]

Identifier: 150398027

GI number: 150398027

Start: 2954038

End: 2955432

Strand: Direct

Name: lpdV [H]

Synonym: Smed_2829

Alternate gene names: 150398027

Gene position: 2954038-2955432 (Clockwise)

Preceding gene: 150398026

Following gene: 150398032

Centisome position: 78.11

GC content: 65.09

Gene sequence:

>1395_bases
ATGAAGGAAATCTCCTGCAAACTCCTGGTTCTTGGCGCCGGTCCCGGCGGCTATGTCACGGCGATCCGCGCCGCCCAGCT
CGGCGTCAACACCGTGATCGTCGAGAAGGCGAAGGCCGGCGGCACCTGCCTGAATGTCGGCTGTATTCCATCCAAGGCGC
TGATCCATGCGGCGGAAGAATACGACAAGCTCCGCACGGCCGCATCGGGTAAGAGCCCGCTCGGCCTCTCCTTGAATGCT
CCCGCAATCGACCTCGGCCGAACGATAGCCTGGAAGGACGGTATCGTCGGGCGGCTCAACAGCGGCGTCACAGGGCTCCT
GAAAAAGGCGGGCGTCAAGGCGGTTATCGGCGAGGGGCGTTTCGTCGACGGCAAGACCGTGGATGTGGAAACGGAGACGG
GCCTCCAGCGCATTCGAGCGGAAGCGCTCGTCATCGCGACCGGTTCGGCGCCGATCGAGCTCCCCGACCTGCCCTTCGGC
GGAAATGTGATTTCCTCGACCCAGGCTTTGGCGCTGACGAAAATACCCGAAACGCTCGCGGTCGTCGGTGGCGGCTATAT
CGGCCTCGAACTCGGCACCGCCTTTGCCAAGCTCGGTTCCAAGGTCACCGTGCTCGAAGCGTTGGACAGAATCCTGCCGC
AATACGACGCCGATCTCTCGAAGCCGGTGATGAAGCGCCTCGGCGAACTCGGTGTGGAGGTCTTCACGGGCACCGCGGCA
AAGCGGCTCTCCGCCGATCGGCGCGGGCTCTTTGCCGAGGAAAACGGCCGCGCCTTCGAGGTCCCGGCGGAGAAGGTCCT
CGTCACCGTCGGTCGCGGGCCTGTCACCGAGGGCTGGGGACTTGAAGAGATCGATCTCGACCGGTCGGGCGGATTCATCC
GCATCGACGATCAGTGCCGCACTTCGATGCGCGGTATCTATGCGATCGGCGACGTGACCGGCGAGCCGATGCTTGCGCAC
CGCGCAATGGCACAGGGCGAAATGGTCGCGGAGATCGTCGCCGGTCGAAAGCGGAGCTGGGACAAGCGCTGCATCCCGGC
GGTTTGCTTCACCGATCCGGAAATCGTCGGCGCCGGCCTCTCGCCGGAAGAGGCTCGCACGGCCGGCATCGATACCAGGA
TCGGCCAGTTTCCTTTCCAGGCGAACGGCCGTGCGATGACAACGCTTTCCGAGGACGGCTTCGTCCGCGTGGTCGCCCGG
GCCGACAATCACCTGGTCCTCGGCATCCAGGCGGTCGGCCACGGCGTTTCGGAACTGTCGGCGACCTTTGCGCTGGCGAT
CGAAATGGGCGCGCGGCTGGAGGATATCGCCGGCACCGTCCATGCGCATCCGACGCAGTCCGAGGCGTTTCAGGAGGCCG
CCTTCAAGGCGCTGGGGCACGCGCTGCATATCTGA

Upstream 100 bases:

>100_bases
ACCACCGGGTGATCGACGGCTGGGATGCCGCGGTTTTCGTTCAGCGGCTGAAGACGCTGATCGAAACGCCGGCGCTGATT
TTCGTTGAAGGATAGGCGTT

Downstream 100 bases:

>100_bases
GGCGGAGCCCGAACACCTCTCCCGATCCCTCAGCCCATCCGTTCGGAGGCGTAGCTTCCCGGGCTCGGCGGGAAGACGAC
GACGCGGTTGCCGTTGATGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKEISCKLLVLGAGPGGYVTAIRAAQLGVNTVIVEKAKAGGTCLNVGCIPSKALIHAAEEYDKLRTAASGKSPLGLSLNA
PAIDLGRTIAWKDGIVGRLNSGVTGLLKKAGVKAVIGEGRFVDGKTVDVETETGLQRIRAEALVIATGSAPIELPDLPFG
GNVISSTQALALTKIPETLAVVGGGYIGLELGTAFAKLGSKVTVLEALDRILPQYDADLSKPVMKRLGELGVEVFTGTAA
KRLSADRRGLFAEENGRAFEVPAEKVLVTVGRGPVTEGWGLEEIDLDRSGGFIRIDDQCRTSMRGIYAIGDVTGEPMLAH
RAMAQGEMVAEIVAGRKRSWDKRCIPAVCFTDPEIVGAGLSPEEARTAGIDTRIGQFPFQANGRAMTTLSEDGFVRVVAR
ADNHLVLGIQAVGHGVSELSATFALAIEMGARLEDIAGTVHAHPTQSEAFQEAAFKALGHALHI

Sequences:

>Translated_464_residues
MKEISCKLLVLGAGPGGYVTAIRAAQLGVNTVIVEKAKAGGTCLNVGCIPSKALIHAAEEYDKLRTAASGKSPLGLSLNA
PAIDLGRTIAWKDGIVGRLNSGVTGLLKKAGVKAVIGEGRFVDGKTVDVETETGLQRIRAEALVIATGSAPIELPDLPFG
GNVISSTQALALTKIPETLAVVGGGYIGLELGTAFAKLGSKVTVLEALDRILPQYDADLSKPVMKRLGELGVEVFTGTAA
KRLSADRRGLFAEENGRAFEVPAEKVLVTVGRGPVTEGWGLEEIDLDRSGGFIRIDDQCRTSMRGIYAIGDVTGEPMLAH
RAMAQGEMVAEIVAGRKRSWDKRCIPAVCFTDPEIVGAGLSPEEARTAGIDTRIGQFPFQANGRAMTTLSEDGFVRVVAR
ADNHLVLGIQAVGHGVSELSATFALAIEMGARLEDIAGTVHAHPTQSEAFQEAAFKALGHALHI
>Mature_464_residues
MKEISCKLLVLGAGPGGYVTAIRAAQLGVNTVIVEKAKAGGTCLNVGCIPSKALIHAAEEYDKLRTAASGKSPLGLSLNA
PAIDLGRTIAWKDGIVGRLNSGVTGLLKKAGVKAVIGEGRFVDGKTVDVETETGLQRIRAEALVIATGSAPIELPDLPFG
GNVISSTQALALTKIPETLAVVGGGYIGLELGTAFAKLGSKVTVLEALDRILPQYDADLSKPVMKRLGELGVEVFTGTAA
KRLSADRRGLFAEENGRAFEVPAEKVLVTVGRGPVTEGWGLEEIDLDRSGGFIRIDDQCRTSMRGIYAIGDVTGEPMLAH
RAMAQGEMVAEIVAGRKRSWDKRCIPAVCFTDPEIVGAGLSPEEARTAGIDTRIGQFPFQANGRAMTTLSEDGFVRVVAR
ADNHLVLGIQAVGHGVSELSATFALAIEMGARLEDIAGTVHAHPTQSEAFQEAAFKALGHALHI

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=38.5438972162741, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI50301238, Length=466, Percent_Identity=30.0429184549356, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI148277065, Length=429, Percent_Identity=26.5734265734266, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI33519430, Length=429, Percent_Identity=26.5734265734266, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI33519428, Length=429, Percent_Identity=26.5734265734266, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI33519426, Length=429, Percent_Identity=26.5734265734266, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI148277071, Length=429, Percent_Identity=26.5734265734266, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI22035672, Length=459, Percent_Identity=29.6296296296296, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI291045266, Length=433, Percent_Identity=26.0969976905312, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI291045268, Length=424, Percent_Identity=25.2358490566038, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1786307, Length=465, Percent_Identity=40.2150537634409, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1789915, Length=436, Percent_Identity=31.4220183486239, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI87081717, Length=453, Percent_Identity=26.0485651214128, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI87082354, Length=464, Percent_Identity=25.6465517241379, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=460, Percent_Identity=38.0434782608696, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=26.8348623853211, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=26.8348623853211, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17557007, Length=477, Percent_Identity=26.8343815513627, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=453, Percent_Identity=24.9448123620309, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321091, Length=479, Percent_Identity=37.5782881002088, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=27.5423728813559, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325166, Length=462, Percent_Identity=28.7878787878788, Blast_Score=153, Evalue=7e-38,
Organism=Drosophila melanogaster, GI21358499, Length=463, Percent_Identity=40.8207343412527, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24640549, Length=481, Percent_Identity=26.8191268191268, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24640553, Length=481, Percent_Identity=26.8191268191268, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24640551, Length=481, Percent_Identity=26.4033264033264, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17737741, Length=471, Percent_Identity=25.9023354564756, Blast_Score=99, Evalue=9e-21,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48633; Mature: 48633

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEISCKLLVLGAGPGGYVTAIRAAQLGVNTVIVEKAKAGGTCLNVGCIPSKALIHAAEE
CCCCEEEEEEEECCCCCHHHEEHHHHCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHH
YDKLRTAASGKSPLGLSLNAPAIDLGRTIAWKDGIVGRLNSGVTGLLKKAGVKAVIGEGR
HHHHHHHCCCCCCCCEECCCCCHHCCCEEEECCCCCCCCCCCHHHHHHHCCCEEEECCCE
FVDGKTVDVETETGLQRIRAEALVIATGSAPIELPDLPFGGNVISSTQALALTKIPETLA
EECCCEEEEEHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEHHCCHHHH
VVGGGYIGLELGTAFAKLGSKVTVLEALDRILPQYDADLSKPVMKRLGELGVEVFTGTAA
HHCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECHHH
KRLSADRRGLFAEENGRAFEVPAEKVLVTVGRGPVTEGWGLEEIDLDRSGGFIRIDDQCR
HHHCHHHCCCEECCCCCEEECCCCCEEEEECCCCCCCCCCCCEEEECCCCCEEEECHHHH
TSMRGIYAIGDVTGEPMLAHRAMAQGEMVAEIVAGRKRSWDKRCIPAVCFTDPEIVGAGL
HHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHEECCC
SPEEARTAGIDTRIGQFPFQANGRAMTTLSEDGFVRVVARADNHLVLGIQAVGHGVSELS
CHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCEEEEEEHHHCCCHHHHH
ATFALAIEMGARLEDIAGTVHAHPTQSEAFQEAAFKALGHALHI
HHHHHHHHHCCCHHHHCCCEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEISCKLLVLGAGPGGYVTAIRAAQLGVNTVIVEKAKAGGTCLNVGCIPSKALIHAAEE
CCCCEEEEEEEECCCCCHHHEEHHHHCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHH
YDKLRTAASGKSPLGLSLNAPAIDLGRTIAWKDGIVGRLNSGVTGLLKKAGVKAVIGEGR
HHHHHHHCCCCCCCCEECCCCCHHCCCEEEECCCCCCCCCCCHHHHHHHCCCEEEECCCE
FVDGKTVDVETETGLQRIRAEALVIATGSAPIELPDLPFGGNVISSTQALALTKIPETLA
EECCCEEEEEHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEHHCCHHHH
VVGGGYIGLELGTAFAKLGSKVTVLEALDRILPQYDADLSKPVMKRLGELGVEVFTGTAA
HHCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECHHH
KRLSADRRGLFAEENGRAFEVPAEKVLVTVGRGPVTEGWGLEEIDLDRSGGFIRIDDQCR
HHHCHHHCCCEECCCCCEEECCCCCEEEEECCCCCCCCCCCCEEEECCCCCEEEECHHHH
TSMRGIYAIGDVTGEPMLAHRAMAQGEMVAEIVAGRKRSWDKRCIPAVCFTDPEIVGAGL
HHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHEECCC
SPEEARTAGIDTRIGQFPFQANGRAMTTLSEDGFVRVVARADNHLVLGIQAVGHGVSELS
CHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCEEEEEEHHHCCCHHHHH
ATFALAIEMGARLEDIAGTVHAHPTQSEAFQEAAFKALGHALHI
HHHHHHHHHCCCHHHHCCCEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]